>KMC001478A_C01 KMC001478A_c01
aGAATTAACTACTACGGTTTCAATCAGTAACAAAAATATTAAACCACCGATAATATGCCA
CCAATAGAAAAAGCAAAAACCAATTATTCAAGTGAACACTCAAAACACAGGTAACGGCAC
CCGCTCTAACGTTATCACTGGGCCGGGTGGAAAACACCACAAGCACAATGGGGCATGTCG
ATGATCGATAGAACAGAGACAAATACAAAGAGCTTATCAGTACATGCTAAATCAAACTTA
TGAGAATACAAAGTAATAATATCTCAAAGAAAATAGCAAATGGTACAGAAGCTAAAAGAC
AGCATTATATCTTTTAGCAGCACTTATTCCTCCTCTTCAGCCTCCTCCAGCCAATTAACA
AACGGTTCCAGTGCCTTCAAAAAGTTCTGCCTGCCCTTGGAGTTTGTTCCTTTTCGGAAC
CAATGCAGAATAGTATCTTCAGCCAGCACATCCTGGTCATAAAGAGATCTAACAATCTCA
GGGAACAGCTTCATCAGTTTGGCATCCTCATAGCATTGAGTCTGTACTTTGTACAACAGT
TCCAGCTCAAGCTTCCCAGTGGTGCAGAATGTATTCAACAGTTCTGCCCAAGTTTTTACC
TGGCGCAAGGCTGAATTTGCATTCTGTTGCTGATTTTTTCCTGACCACTGAACAGCATCC
ATTAAAACATCCCACAAGACCCGCA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001478A_C01 KMC001478A_c01
         (685 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_568534.1| putative protein; protein id: At5g36230.1, supp...   224  1e-57
dbj|BAB09363.1| gb|AAD26879.1~gene_id:T30G6.9~strong similarity ...   224  1e-57
ref|NP_564845.1| expressed protein; protein id: At1g65220.1, sup...   208  6e-53
gb|AAM63296.1| unknown [Arabidopsis thaliana]                         208  6e-53
pir||C96676 hypothetical protein T23K8.13 [imported] - Arabidops...   208  6e-53

>ref|NP_568534.1| putative protein; protein id: At5g36230.1, supported by cDNA:
           gi_16323076 [Arabidopsis thaliana]
           gi|16323077|gb|AAL15273.1| AT5g36230/T30G6_9
           [Arabidopsis thaliana] gi|22531269|gb|AAM97138.1|
           putative protein [Arabidopsis thaliana]
          Length = 411

 Score =  224 bits (570), Expect = 1e-57
 Identities = 105/119 (88%), Positives = 113/119 (94%)
 Frame = -3

Query: 683 RVLWDVLMDAVQWSGKNQQQNANSALRQVKTWAELLNTFCTTGKLELELLYKVQTQCYED 504
           RV+WD LMDAVQWSGKNQQQNANS LRQVKTWA LLNTFCT+GKLELEL+YKVQ QCYED
Sbjct: 293 RVVWDGLMDAVQWSGKNQQQNANSVLRQVKTWAPLLNTFCTSGKLELELMYKVQMQCYED 352

Query: 503 AKLMKLFPEIVRSLYDQDVLAEDTILHWFRKGTNSKGRQNFLKALEPFVNWLEEAEEEE 327
           AKLMK+FPE+VRSLY+ DVLAEDTILHWFRKGTNSKGRQ F+K+LEPFVNWLEEAEEEE
Sbjct: 353 AKLMKVFPEVVRSLYELDVLAEDTILHWFRKGTNSKGRQTFVKSLEPFVNWLEEAEEEE 411

>dbj|BAB09363.1| gb|AAD26879.1~gene_id:T30G6.9~strong similarity to unknown protein
           [Arabidopsis thaliana]
          Length = 429

 Score =  224 bits (570), Expect = 1e-57
 Identities = 105/119 (88%), Positives = 113/119 (94%)
 Frame = -3

Query: 683 RVLWDVLMDAVQWSGKNQQQNANSALRQVKTWAELLNTFCTTGKLELELLYKVQTQCYED 504
           RV+WD LMDAVQWSGKNQQQNANS LRQVKTWA LLNTFCT+GKLELEL+YKVQ QCYED
Sbjct: 311 RVVWDGLMDAVQWSGKNQQQNANSVLRQVKTWAPLLNTFCTSGKLELELMYKVQMQCYED 370

Query: 503 AKLMKLFPEIVRSLYDQDVLAEDTILHWFRKGTNSKGRQNFLKALEPFVNWLEEAEEEE 327
           AKLMK+FPE+VRSLY+ DVLAEDTILHWFRKGTNSKGRQ F+K+LEPFVNWLEEAEEEE
Sbjct: 371 AKLMKVFPEVVRSLYELDVLAEDTILHWFRKGTNSKGRQTFVKSLEPFVNWLEEAEEEE 429

>ref|NP_564845.1| expressed protein; protein id: At1g65220.1, supported by cDNA:
           21223., supported by cDNA: gi_15810284 [Arabidopsis
           thaliana] gi|15810285|gb|AAL07030.1| unknown protein
           [Arabidopsis thaliana]
          Length = 411

 Score =  208 bits (529), Expect = 6e-53
 Identities = 94/118 (79%), Positives = 108/118 (90%)
 Frame = -3

Query: 680 VLWDVLMDAVQWSGKNQQQNANSALRQVKTWAELLNTFCTTGKLELELLYKVQTQCYEDA 501
           V+WD +M+AVQWSGKNQQQN+N+ LRQVKTWA LLNT C+TG +E+EL+YKVQ QCYEDA
Sbjct: 294 VIWDGIMNAVQWSGKNQQQNSNAVLRQVKTWAPLLNTLCSTGNMEMELMYKVQMQCYEDA 353

Query: 500 KLMKLFPEIVRSLYDQDVLAEDTILHWFRKGTNSKGRQNFLKALEPFVNWLEEAEEEE 327
           KLMK+FPE+VRSLY+ DVLAEDTILHW+RKGTN KGRQ F+K LEPFVNWLEEAEEEE
Sbjct: 354 KLMKVFPEVVRSLYELDVLAEDTILHWYRKGTNPKGRQTFVKGLEPFVNWLEEAEEEE 411

>gb|AAM63296.1| unknown [Arabidopsis thaliana]
          Length = 411

 Score =  208 bits (529), Expect = 6e-53
 Identities = 94/118 (79%), Positives = 108/118 (90%)
 Frame = -3

Query: 680 VLWDVLMDAVQWSGKNQQQNANSALRQVKTWAELLNTFCTTGKLELELLYKVQTQCYEDA 501
           V+WD +M+AVQWSGKNQQQN+N+ LRQVKTWA LLNT C+TG +E+EL+YKVQ QCYEDA
Sbjct: 294 VIWDRIMNAVQWSGKNQQQNSNAVLRQVKTWAPLLNTLCSTGNMEMELMYKVQMQCYEDA 353

Query: 500 KLMKLFPEIVRSLYDQDVLAEDTILHWFRKGTNSKGRQNFLKALEPFVNWLEEAEEEE 327
           KLMK+FPE+VRSLY+ DVLAEDTILHW+RKGTN KGRQ F+K LEPFVNWLEEAEEEE
Sbjct: 354 KLMKVFPEVVRSLYELDVLAEDTILHWYRKGTNPKGRQTFVKGLEPFVNWLEEAEEEE 411

>pir||C96676 hypothetical protein T23K8.13 [imported] - Arabidopsis thaliana
           gi|4646206|gb|AAD26879.1|AC007230_13 Contains similarity
           to gb|D13630 KIAA0005 gene from Homo sapiens.  ESTs
           gb|T45345, gb|T21086, gb|R90360, gb|T20468, gb|T45191
           and gb|AI100459 come from this gene. [Arabidopsis
           thaliana]
          Length = 426

 Score =  208 bits (529), Expect = 6e-53
 Identities = 94/118 (79%), Positives = 108/118 (90%)
 Frame = -3

Query: 680 VLWDVLMDAVQWSGKNQQQNANSALRQVKTWAELLNTFCTTGKLELELLYKVQTQCYEDA 501
           V+WD +M+AVQWSGKNQQQN+N+ LRQVKTWA LLNT C+TG +E+EL+YKVQ QCYEDA
Sbjct: 309 VIWDGIMNAVQWSGKNQQQNSNAVLRQVKTWAPLLNTLCSTGNMEMELMYKVQMQCYEDA 368

Query: 500 KLMKLFPEIVRSLYDQDVLAEDTILHWFRKGTNSKGRQNFLKALEPFVNWLEEAEEEE 327
           KLMK+FPE+VRSLY+ DVLAEDTILHW+RKGTN KGRQ F+K LEPFVNWLEEAEEEE
Sbjct: 369 KLMKVFPEVVRSLYELDVLAEDTILHWYRKGTNPKGRQTFVKGLEPFVNWLEEAEEEE 426

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 586,471,274
Number of Sequences: 1393205
Number of extensions: 12752749
Number of successful extensions: 50419
Number of sequences better than 10.0: 127
Number of HSP's better than 10.0 without gapping: 41773
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 46799
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 30552968016
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPDL023e03_f BP053440 1 576 2 MFBL023d01_f BP042405 19 326 3 MWM082e03_f AV766070 19 377 4 GENf001c04 BP058344 25 424 5 MR066h10_f BP081115 39 447 6 MF063a09_f BP031617 52 329 7 GENf039e04 BP060028 56 490 8 GENf035d03 BP059840 56 475 9 GENf039a09 BP060010 57 489 10 MPD048b05_f AV773232 58 193 11 GENf098d01 BP062341 59 304 12 SPD049d02_f BP047900 59 603 13 MR014a09_f BP076999 59 363 14 GNf060b07 BP071807 60 638 15 SPDL040c07_f BP054508 74 340 16 MF012f01_f BP028878 75 642 17 GNf041e06 BP070394 76 527 18 GNLf003a12 BP074943 78 148 19 MR092h11_f BP083119 78 447 20 GNf015a08 BP068426 80 628 21 GENf055h08 BP060713 84 267 22 SPD038b02_f BP046993 84 511 23 GNf061g06 BP071930 84 634 24 GNf010g06 BP068113 84 194 25 MFBL021a01_f BP042286 84 687 26 GNf018b02 BP068649 84 573 27 SPD041a08_f BP047229 84 681 28 MR072e01_f BP081541 84 695 29 GENf021g01 BP059270 84 475 30 MR011c01_f BP076768 84 532 31 GNf051h06 BP071189 84 585 32 MR076a07_f BP081816 85 536 33 GNf037b06 BP070039 85 570 34 MWL068g11_f AV769801 85 705 35 SPD042e08_f BP047355 86 568 36 MR078h12_f BP082044 87 534 37 MRL035d03_f BP085441 87 514 38 SPDL049h11_f BP055112 87 680 39 SPD094e09_f BP051517 88 329 40 MFB098h09_f BP041165 88 636 41 SPDL070e09_f BP056348 102 690 42 SPD054e12_f BP048319 103 722 43 MR049c06_f BP079778 104 595 44 MFB038f11_f BP036806 136 765 45 GENf061c05 BP060943 250 758 46 GENf019d03 BP059160 253 638

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