>KMC001444A_C01 KMC001444A_c01
GAAGAACCCAAACCTTAACTGAATCAATCCAAGACTTGACCATTCAACAACCCAATCGTT
TTCTTCCATTCCTTCCTTCCACCGCACACTCGCTTCCCCTTCCGATTCTCTCATCAACAA
CCTGTGGATTGAGAATATGGATCTAGGAGGGAGTGAGAAGGAATCAGTGTTTACACAAGT
GAGCATTGGAGGTTTTGATAGTGAAGTGAAAGCACATGATCTTGCTGAGTTTCTGGAAAA
TAGAATTGGACTTGTTTACAGATGCAGGCTGAAAACATCTTGGACACCGCCGGAGTCCTA
TCCGGAATTTAATGTCGCGGATACTGCATTGGTTAAGAGGACAGATGACTACAAGAAGGT
GGAGCCTCATGCCTTTGTGCATTTTGCGTTGCCGGAATCTGCGACTGAGGCGCTTGATGC
TTCGGGTAGTTGTGATCTAATGTGGGAGGGCAAACCGTTGAAGGTTATCAGCGGGCCTCA
GAATCCGTACTTTTTGAACCAGAGGAGGAGAAAGGAGACCCCTTTTAAGCTGTCTGATGT
GCTTGTTGAAATTGGGAC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001444A_C01 KMC001444A_c01
         (558 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAF74208.1|AF268093_1 RNA-dependent RNA polymerase [Arabidops...   176  1e-43
ref|NP_190519.1| RNA-dependent RNA polymerase SDE1; protein id: ...   176  1e-43
dbj|BAC00725.1| putative RNA-directed RNA polymerase [Oryza sati...   130  8e-30
gb|AAO11660.1| hypothetical protein [Arabidopsis thaliana]            113  2e-24
ref|NP_178605.1| hypothetical protein; protein id: At2g05370.1 [...   110  1e-23

>gb|AAF74208.1|AF268093_1 RNA-dependent RNA polymerase [Arabidopsis thaliana]
          Length = 1196

 Score =  176 bits (447), Expect = 1e-43
 Identities = 81/136 (59%), Positives = 105/136 (76%)
 Frame = +2

Query: 149 GSEKESVFTQVSIGGFDSEVKAHDLAEFLENRIGLVYRCRLKTSWTPPESYPEFNVADTA 328
           G+ K+SV TQVSIGGF     A  L ++LE+ +G+V+RCRLKTSWTPP SYP F +ADT+
Sbjct: 5   GNMKKSVVTQVSIGGFGESTTAKQLTDYLEDEVGIVWRCRLKTSWTPPGSYPNFEIADTS 64

Query: 329 LVKRTDDYKKVEPHAFVHFALPESATEALDASGSCDLMWEGKPLKVISGPQNPYFLNQRR 508
            +   D+YKKVEPHAFVHFA+ ESA  A+DA+G C+L+ +G+PLKV  GP+NPY LNQRR
Sbjct: 65  NIPSIDEYKKVEPHAFVHFAVFESAGRAMDAAGQCNLILDGQPLKVSLGPKNPYSLNQRR 124

Query: 509 RKETPFKLSDVLVEIG 556
           R   P+KL+ + +EIG
Sbjct: 125 RTTVPYKLAGITLEIG 140

>ref|NP_190519.1| RNA-dependent RNA polymerase SDE1; protein id: At3g49500.1
           [Arabidopsis thaliana] gi|6899955|emb|CAB71285.1|
           RNA-directed RNA polymerase-like protein [Arabidopsis
           thaliana] gi|8164028|gb|AAF73959.1| SGS2 [Arabidopsis
           thaliana] gi|12324444|gb|AAG52184.1|AC012329_11 putative
           RNA-directed RNA polymerase; 73997-69985 [Arabidopsis
           thaliana]
          Length = 1196

 Score =  176 bits (447), Expect = 1e-43
 Identities = 81/136 (59%), Positives = 105/136 (76%)
 Frame = +2

Query: 149 GSEKESVFTQVSIGGFDSEVKAHDLAEFLENRIGLVYRCRLKTSWTPPESYPEFNVADTA 328
           G+ K+SV TQVSIGGF     A  L ++LE+ +G+V+RCRLKTSWTPP SYP F +ADT+
Sbjct: 5   GNMKKSVVTQVSIGGFGESTTAKQLTDYLEDEVGIVWRCRLKTSWTPPGSYPNFEIADTS 64

Query: 329 LVKRTDDYKKVEPHAFVHFALPESATEALDASGSCDLMWEGKPLKVISGPQNPYFLNQRR 508
            +   D+YKKVEPHAFVHFA+ ESA  A+DA+G C+L+ +G+PLKV  GP+NPY LNQRR
Sbjct: 65  NIPSIDEYKKVEPHAFVHFAVFESAGRAMDAAGQCNLILDGQPLKVSLGPKNPYSLNQRR 124

Query: 509 RKETPFKLSDVLVEIG 556
           R   P+KL+ + +EIG
Sbjct: 125 RTTVPYKLAGITLEIG 140

>dbj|BAC00725.1| putative RNA-directed RNA polymerase [Oryza sativa (japonica
           cultivar-group)]
          Length = 1218

 Score =  130 bits (328), Expect = 8e-30
 Identities = 67/134 (50%), Positives = 88/134 (65%), Gaps = 4/134 (2%)
 Frame = +2

Query: 167 VFTQVSIGGFDSEVKAHDLAEFLENRIGLVYRCRLKTSWTPPESYPEFNV----ADTALV 334
           V TQVS+GGFD+ V A DLA+FLE+ +GLV+RCR+KTSWTPP+SYP+F +    A  +  
Sbjct: 28  VTTQVSLGGFDAGVAAGDLADFLEHEVGLVWRCRVKTSWTPPDSYPDFALPTAPASASAA 87

Query: 335 KRTDDYKKVEPHAFVHFALPESATEALDASGSCDLMWEGKPLKVISGPQNPYFLNQRRRK 514
                Y +V PHAFVHFA PE A  A D +G   L+  GKPL+V S P +   ++ RR  
Sbjct: 88  AAPPRYDRVPPHAFVHFARPEGARRAADLAGETRLILRGKPLRVASAPDSSLRVS-RRSS 146

Query: 515 ETPFKLSDVLVEIG 556
             PF+  DV +E+G
Sbjct: 147 IAPFRFPDVRLEVG 160

>gb|AAO11660.1| hypothetical protein [Arabidopsis thaliana]
          Length = 143

 Score =  113 bits (282), Expect = 2e-24
 Identities = 61/141 (43%), Positives = 87/141 (61%), Gaps = 1/141 (0%)
 Frame = +2

Query: 137 MDLGGSEKESVFTQVSIGGFDSEVKAHDLAEFLENRIGL-VYRCRLKTSWTPPESYPEFN 313
           M+  G+ K  V +QV+I GF    K  +L ++LEN++GL V+RCRLKTSWTP  SYP F 
Sbjct: 1   MESEGNMKNLVVSQVTISGFGESTKTKELTDYLENKVGLLVWRCRLKTSWTPHGSYPNFE 60

Query: 314 VADTALVKRTDDYKKVEPHAFVHFALPESATEALDASGSCDLMWEGKPLKVISGPQNPYF 493
           + DT+ + + D Y++V PHAFVH    ++    +DA+    L+  G+PLK     +NPY 
Sbjct: 61  IIDTSNIPKFDCYERVVPHAFVHC---DAHKSLMDAAEQXKLILGGQPLKFSLSSENPYT 117

Query: 494 LNQRRRKETPFKLSDVLVEIG 556
           LN       P+KL+ V +EIG
Sbjct: 118 LN-------PYKLTGVSLEIG 131

>ref|NP_178605.1| hypothetical protein; protein id: At2g05370.1 [Arabidopsis
           thaliana] gi|25371231|pir||F84467 hypothetical protein
           At2g05370 [imported] - Arabidopsis thaliana
           gi|4914363|gb|AAD32900.1| hypothetical protein
           [Arabidopsis thaliana]
          Length = 211

 Score =  110 bits (275), Expect = 1e-23
 Identities = 59/134 (44%), Positives = 83/134 (61%), Gaps = 1/134 (0%)
 Frame = +2

Query: 158 KESVFTQVSIGGFDSEVKAHDLAEFLENRIGL-VYRCRLKTSWTPPESYPEFNVADTALV 334
           K  V +QV+I GF    K  +L ++LEN++GL V+RCRLKTSWTP  SYP F + DT+ +
Sbjct: 2   KNLVVSQVTISGFGESTKTKELTDYLENKVGLLVWRCRLKTSWTPHGSYPNFEIIDTSNI 61

Query: 335 KRTDDYKKVEPHAFVHFALPESATEALDASGSCDLMWEGKPLKVISGPQNPYFLNQRRRK 514
            + D Y++V PHAFVH    ++    +DA+    L+  G+PLK     +NPY LN     
Sbjct: 62  PKFDCYERVVPHAFVHC---DAHKSLMDAAEQSKLILGGQPLKFSLSSENPYTLN----- 113

Query: 515 ETPFKLSDVLVEIG 556
             P+KL+ V +EIG
Sbjct: 114 --PYKLTGVSLEIG 125

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 499,243,477
Number of Sequences: 1393205
Number of extensions: 10741725
Number of successful extensions: 30493
Number of sequences better than 10.0: 61
Number of HSP's better than 10.0 without gapping: 29163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30445
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19808345223
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENLf090b01 BP067243 1 534 2 MPDL004h01_f AV776742 61 618

---