Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001438A_C01 KMC001438A_c01
(720 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAC44451.1| proton-translocating inorganic pyrophosphatase [... 81 2e-29
dbj|BAC41250.1| vacuolar proton-inorganic pyrophosphatase [Pyrus... 94 1e-25
emb|CAC39165.1| vacuolar-type H+-pyrophosphatase [Lycopersicon e... 94 1e-25
pir||S61425 inorganic diphosphatase (EC 3.6.1.1), H+-translocati... 93 2e-25
pir||T14564 inorganic diphosphatase (EC 3.6.1.1), vacuolar - bee... 94 2e-25
>emb|CAC44451.1| proton-translocating inorganic pyrophosphatase [Chlamydomonas
reinhardtii]
Length = 762
Score = 81.3 bits (199), Expect(2) = 2e-29
Identities = 39/47 (82%), Positives = 43/47 (90%)
Frame = -2
Query: 674 LSGVLAGSLVSGVQIAISASNPGGAWDNAKKYIEAGASEHARTLGPK 534
L+GVLAGSLVSGVQ+A+S SN GGAWDNAKKYIEAGA+EHAR LG K
Sbjct: 655 LAGVLAGSLVSGVQLAVSMSNTGGAWDNAKKYIEAGATEHARELGGK 701
Score = 70.5 bits (171), Expect(2) = 2e-29
Identities = 35/46 (76%), Positives = 37/46 (80%)
Frame = -3
Query: 532 GSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAG*VTCFCPIFRN 395
GSD HKAAVIGDT+GDPLKDT+GPSLNILIKLMA F P F N
Sbjct: 702 GSDCHKAAVIGDTVGDPLKDTNGPSLNILIKLMAVESLVFAPFFYN 747
>dbj|BAC41250.1| vacuolar proton-inorganic pyrophosphatase [Pyrus communis]
Length = 767
Score = 94.4 bits (233), Expect(2) = 1e-25
Identities = 47/48 (97%), Positives = 47/48 (97%)
Frame = -2
Query: 677 TLSGVLAGSLVSGVQIAISASNPGGAWDNAKKYIEAGASEHARTLGPK 534
TLSGVLAGSLVSGVQIAISASN GGAWDNAKKYIEAGASEHARTLGPK
Sbjct: 665 TLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPK 712
Score = 75.1 bits (183), Expect = 9e-13
Identities = 40/55 (72%), Positives = 40/55 (72%)
Frame = -3
Query: 565 HLSMQGPWAQSGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAG*VTCFCPIF 401
H GP GSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMA F P F
Sbjct: 705 HARTLGP---KGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFF 756
Score = 44.7 bits (104), Expect(2) = 1e-25
Identities = 21/26 (80%), Positives = 22/26 (83%)
Frame = -1
Query: 447 LLSSWPVESLVFAPFFATHGGLLFKI 370
L+ VESLVFAPFFATHGGLLFKI
Sbjct: 741 LIKLMAVESLVFAPFFATHGGLLFKI 766
>emb|CAC39165.1| vacuolar-type H+-pyrophosphatase [Lycopersicon esculentum]
Length = 356
Score = 94.4 bits (233), Expect(2) = 1e-25
Identities = 47/48 (97%), Positives = 47/48 (97%)
Frame = -2
Query: 677 TLSGVLAGSLVSGVQIAISASNPGGAWDNAKKYIEAGASEHARTLGPK 534
TLSGVLAGSLVSGVQIAISASN GGAWDNAKKYIEAGASEHARTLGPK
Sbjct: 254 TLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPK 301
Score = 71.6 bits (174), Expect = 1e-11
Identities = 38/55 (69%), Positives = 39/55 (70%)
Frame = -3
Query: 565 HLSMQGPWAQSGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAG*VTCFCPIF 401
H GP GSD HKAAVIGDT+GDPLKDTSGPSLNILIKLMA F P F
Sbjct: 294 HARTLGP---KGSDAHKAAVIGDTVGDPLKDTSGPSLNILIKLMAVESLVFAPFF 345
Score = 44.3 bits (103), Expect(2) = 1e-25
Identities = 30/68 (44%), Positives = 37/68 (54%), Gaps = 7/68 (10%)
Frame = -1
Query: 552 KDLGPKAGLTHTRQLLLVTPLETL-------LRILLALHSTSLLSSWPVESLVFAPFFAT 394
+ LGPK H ++ T + L L IL+ L + VESLVFAPFFAT
Sbjct: 296 RTLGPKGSDAHKAAVIGDTVGDPLKDTSGPSLNILIKLMA--------VESLVFAPFFAT 347
Query: 393 HGGLLFKI 370
HGGLLFK+
Sbjct: 348 HGGLLFKL 355
>pir||S61425 inorganic diphosphatase (EC 3.6.1.1), H+-translocating (clone
TVP17), vacuolar membrane - common tobacco (fragment)
gi|790475|emb|CAA58699.1| inorganic pyrophosphatase
[Nicotiana tabacum]
Length = 541
Score = 92.8 bits (229), Expect(2) = 2e-25
Identities = 46/48 (95%), Positives = 46/48 (95%)
Frame = -2
Query: 677 TLSGVLAGSLVSGVQIAISASNPGGAWDNAKKYIEAGASEHARTLGPK 534
TLSGVLAGSLVSGVQIAISASN GGAWDNAKKYIEAG SEHARTLGPK
Sbjct: 439 TLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHARTLGPK 486
Score = 68.9 bits (167), Expect = 7e-11
Identities = 37/55 (67%), Positives = 38/55 (68%)
Frame = -3
Query: 565 HLSMQGPWAQSGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAG*VTCFCPIF 401
H GP GS HKAAVIGDT+GDPLKDTSGPSLNILIKLMA F P F
Sbjct: 479 HARTLGP---KGSTAHKAAVIGDTVGDPLKDTSGPSLNILIKLMAVESLVFAPFF 530
Score = 45.4 bits (106), Expect(2) = 2e-25
Identities = 30/68 (44%), Positives = 37/68 (54%), Gaps = 7/68 (10%)
Frame = -1
Query: 552 KDLGPKAGLTHTRQLLLVTPLETL-------LRILLALHSTSLLSSWPVESLVFAPFFAT 394
+ LGPK H ++ T + L L IL+ L + VESLVFAPFFAT
Sbjct: 481 RTLGPKGSTAHKAAVIGDTVGDPLKDTSGPSLNILIKLMA--------VESLVFAPFFAT 532
Query: 393 HGGLLFKI 370
HGGLLFK+
Sbjct: 533 HGGLLFKL 540
>pir||T14564 inorganic diphosphatase (EC 3.6.1.1), vacuolar - beet
gi|485744|gb|AAA61610.1| pyrophosphatase [Beta vulgaris]
Length = 765
Score = 94.4 bits (233), Expect(2) = 2e-25
Identities = 47/48 (97%), Positives = 47/48 (97%)
Frame = -2
Query: 677 TLSGVLAGSLVSGVQIAISASNPGGAWDNAKKYIEAGASEHARTLGPK 534
TLSGVLAGSLVSGVQIAISASN GGAWDNAKKYIEAGASEHARTLGPK
Sbjct: 663 TLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPK 710
Score = 72.0 bits (175), Expect = 8e-12
Identities = 39/55 (70%), Positives = 39/55 (70%)
Frame = -3
Query: 565 HLSMQGPWAQSGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAG*VTCFCPIF 401
H GP GSD HKAAVIGDTIGDPLKDTSGPSLNILIKLMA F P F
Sbjct: 703 HARTLGP---KGSDAHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFF 754
Score = 43.5 bits (101), Expect(2) = 2e-25
Identities = 27/60 (45%), Positives = 33/60 (55%)
Frame = -1
Query: 552 KDLGPKAGLTHTRQLLLVTPLETLLRILLALHSTSLLSSWPVESLVFAPFFATHGGLLFK 373
+ LGPK H ++ T + L+ L+ VESLVFAPFFATHGGLLFK
Sbjct: 705 RTLGPKGSDAHKAAVIGDT-IGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFK 763
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 636,624,192
Number of Sequences: 1393205
Number of extensions: 14230376
Number of successful extensions: 41656
Number of sequences better than 10.0: 128
Number of HSP's better than 10.0 without gapping: 39624
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 41495
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 33499052993
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
