Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001373A_C01 KMC001373A_c01
(491 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_192770.1| isoleucine-tRNA ligase - like protein; protein ... 255 2e-67
sp|P36422|SYI_TETTH ISOLEUCYL-TRNA SYNTHETASE (ISOLEUCINE--TRNA ... 194 4e-49
gb|AAA34712.1| isoleucyl-tRNA synthetase (EC 6.1.1.5) 191 3e-48
ref|NP_009477.1| cytoplasmic isoleucyl-tRNA synthetase; Ils1p [S... 191 3e-48
ref|XP_225196.1| similar to Isoleucyl-tRNA synthetase, cytoplasm... 186 9e-47
>ref|NP_192770.1| isoleucine-tRNA ligase - like protein; protein id: At4g10320.1
[Arabidopsis thaliana] gi|7437548|pir||T04047
isoleucine-tRNA ligase homolog F24G24.120 - Arabidopsis
thaliana gi|4538961|emb|CAB39785.1| isoleucine-tRNA
ligase-like protein [Arabidopsis thaliana]
gi|7267729|emb|CAB78155.1| isoleucine-tRNA ligase-like
protein [Arabidopsis thaliana]
Length = 1254
Score = 255 bits (652), Expect = 2e-67
Identities = 113/138 (81%), Positives = 129/138 (92%)
Frame = +2
Query: 74 MEEVCEGKDFAFPKQEDTILEFWTRIDAFKTQLSLTKDMPEYIFYDGPPFATGLPHYGHI 253
MEEVCEGK+F+FP+QE+ +L FWT IDAFKTQL T+++PEYIFYDGPPFATGLPHYGHI
Sbjct: 1 MEEVCEGKEFSFPRQEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHI 60
Query: 254 LAGTIKDIVTRYQSMTGHHVTRRFGWDCHGLPVENEIDKKLGIKKREDVLKLGIGNYNEE 433
LAGTIKDIVTRYQ+MTGHHVTRRFGWDCHGLPVENEID+KL IK+R++V+K+GI YNEE
Sbjct: 61 LAGTIKDIVTRYQTMTGHHVTRRFGWDCHGLPVENEIDRKLNIKRRDEVIKMGIDKYNEE 120
Query: 434 CRSIVTRYVSEWEAVITR 487
CRSIVTRYV+EWE VITR
Sbjct: 121 CRSIVTRYVAEWEKVITR 138
>sp|P36422|SYI_TETTH ISOLEUCYL-TRNA SYNTHETASE (ISOLEUCINE--TRNA LIGASE) (ILERS)
gi|283498|pir||A42399 isoleucine-tRNA ligase (EC
6.1.1.5) - Tetrahymena thermophila
gi|161801|gb|AAA30122.1| transfer RNA-Ile synthetase
Length = 1081
Score = 194 bits (493), Expect = 4e-49
Identities = 85/127 (66%), Positives = 101/127 (78%)
Frame = +2
Query: 107 FPKQEDTILEFWTRIDAFKTQLSLTKDMPEYIFYDGPPFATGLPHYGHILAGTIKDIVTR 286
FPK+E+ IL+FW I+AFK QL TKD P + FYDGPPFATGLPHYG++LAGTIKD+V R
Sbjct: 16 FPKEEENILKFWDEINAFKQQLEKTKDCPPFTFYDGPPFATGLPHYGNLLAGTIKDVVCR 75
Query: 287 YQSMTGHHVTRRFGWDCHGLPVENEIDKKLGIKKREDVLKLGIGNYNEECRSIVTRYVSE 466
Y S G +V RRFGWDCHGLPVE EIDKKLGI R++VLK+G+ YN ECRSIV RY E
Sbjct: 76 YASQNGKYVERRFGWDCHGLPVEYEIDKKLGITNRQEVLKMGVDKYNAECRSIVMRYAQE 135
Query: 467 WEAVITR 487
W +++ R
Sbjct: 136 WRSIVNR 142
>gb|AAA34712.1| isoleucyl-tRNA synthetase (EC 6.1.1.5)
Length = 1073
Score = 191 bits (486), Expect = 3e-48
Identities = 85/129 (65%), Positives = 99/129 (75%)
Frame = +2
Query: 101 FAFPKQEDTILEFWTRIDAFKTQLSLTKDMPEYIFYDGPPFATGLPHYGHILAGTIKDIV 280
F+FPK+E+ +L W IDAF T L LTKD PE+ F+DGPPFATG PHYGHILA TIKDIV
Sbjct: 8 FSFPKEEEKVLSLWDEIDAFHTSLELTKDKPEFSFFDGPPFATGTPHYGHILASTIKDIV 67
Query: 281 TRYQSMTGHHVTRRFGWDCHGLPVENEIDKKLGIKKREDVLKLGIGNYNEECRSIVTRYV 460
RY +MTGHHV RRFGWD HG+P+E+ IDKKLGI ++DV K G+ NYN ECRSIV Y
Sbjct: 68 PRYATMTGHHVERRFGWDTHGVPIEHIIDKKLGITGKDDVFKYGLENYNNECRSIVMTYA 127
Query: 461 SEWEAVITR 487
S+W I R
Sbjct: 128 SDWRKTIGR 136
>ref|NP_009477.1| cytoplasmic isoleucyl-tRNA synthetase; Ils1p [Saccharomyces
cerevisiae] gi|135134|sp|P09436|SYIC_YEAST
ISOLEUCYL-TRNA SYNTHETASE, CYTOPLASMIC (ISOLEUCINE--TRNA
LIGASE) (ILERS) gi|68524|pir||SYBYI4 isoleucine-tRNA
ligase (EC 6.1.1.5) - yeast (Saccharomyces cerevisiae)
gi|3820|emb|CAA30733.1| ILSI (AA 1-1072) [Saccharomyces
cerevisiae] gi|498991|emb|CAA56034.1| E-1072 protein;
ILS1 protein [Saccharomyces cerevisiae]
gi|536123|emb|CAA84898.1| ORF YBL076c [Saccharomyces
cerevisiae]
Length = 1072
Score = 191 bits (486), Expect = 3e-48
Identities = 85/129 (65%), Positives = 99/129 (75%)
Frame = +2
Query: 101 FAFPKQEDTILEFWTRIDAFKTQLSLTKDMPEYIFYDGPPFATGLPHYGHILAGTIKDIV 280
F+FPK+E+ +L W IDAF T L LTKD PE+ F+DGPPFATG PHYGHILA TIKDIV
Sbjct: 8 FSFPKEEEKVLSLWDEIDAFHTSLELTKDKPEFSFFDGPPFATGTPHYGHILASTIKDIV 67
Query: 281 TRYQSMTGHHVTRRFGWDCHGLPVENEIDKKLGIKKREDVLKLGIGNYNEECRSIVTRYV 460
RY +MTGHHV RRFGWD HG+P+E+ IDKKLGI ++DV K G+ NYN ECRSIV Y
Sbjct: 68 PRYATMTGHHVERRFGWDTHGVPIEHIIDKKLGITGKDDVFKYGLENYNNECRSIVMTYA 127
Query: 461 SEWEAVITR 487
S+W I R
Sbjct: 128 SDWRKTIGR 136
>ref|XP_225196.1| similar to Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA
ligase) (IleRS) (IRS) [Rattus norvegicus]
Length = 2267
Score = 186 bits (473), Expect = 9e-47
Identities = 85/143 (59%), Positives = 110/143 (76%)
Frame = +2
Query: 59 PAASTMEEVCEGKDFAFPKQEDTILEFWTRIDAFKTQLSLTKDMPEYIFYDGPPFATGLP 238
P+ +++V E + +FP +E+ IL+FW++ + F+ L +K P++ FYDGPPFATGLP
Sbjct: 943 PSNRMVQQVPE--NISFPAEEEKILQFWSKYNCFQECLKQSKLRPKFTFYDGPPFATGLP 1000
Query: 239 HYGHILAGTIKDIVTRYQSMTGHHVTRRFGWDCHGLPVENEIDKKLGIKKREDVLKLGIG 418
HYGHILAGTIKDIVTRY +G HV RRFGWDCHGLPVE EIDK LGIK EDV K+GI
Sbjct: 1001 HYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIA 1060
Query: 419 NYNEECRSIVTRYVSEWEAVITR 487
YN++CR+IV RY +EW++ +TR
Sbjct: 1061 EYNKQCRAIVMRYSAEWKSTVTR 1083
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 459,969,802
Number of Sequences: 1393205
Number of extensions: 11799191
Number of successful extensions: 117987
Number of sequences better than 10.0: 6034
Number of HSP's better than 10.0 without gapping: 70661
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 103414
length of database: 448,689,247
effective HSP length: 114
effective length of database: 289,863,877
effective search space used: 14203329973
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
