Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001349A_C01 KMC001349A_c01
(856 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T09153 glucose-6-phosphate isomerase (EC 5.3.1.9) precursor... 166 2e-88
gb|AAN41353.1| putative glucose-6-phosphate isomerase [Arabidops... 174 5e-80
ref|NP_194193.1| glucose-6-phosphate isomerase; protein id: At4g... 170 7e-79
gb|AAF24124.1|AF120494_1 phosphoglucose isomerase precursor [Ara... 158 6e-75
gb|AAO72628.1| glucose-6-phosphate isomerase-like protein [Oryza... 172 2e-73
>pir||T09153 glucose-6-phosphate isomerase (EC 5.3.1.9) precursor, chloroplast -
spinach gi|3413511|emb|CAA03982.1| glucose-6-phosphate
isomerase [Spinacia oleracea]
Length = 618
Score = 166 bits (421), Expect(3) = 2e-88
Identities = 86/101 (85%), Positives = 91/101 (89%), Gaps = 1/101 (0%)
Frame = -1
Query: 442 APNEFLKTQIESTLDAVCNFADDVVSGKIKSPKG-RFTQILSVGIGGSALGPQFVAEALA 266
AP FLK QI+ TLD V FA+DV+SGKIK+P G RFT ILSVGIGGSALGPQFVAEALA
Sbjct: 153 APTTFLKNQIDVTLDRVWQFANDVISGKIKAPTGERFTHILSVGIGGSALGPQFVAEALA 212
Query: 265 PDNPPLKIRFIDNTDPAGIDHQIAQLGPELATTLVIVISKS 143
PDNPPLKIRFIDNTDPAGIDHQIAQLGPELATTLV+VISKS
Sbjct: 213 PDNPPLKIRFIDNTDPAGIDHQIAQLGPELATTLVMVISKS 253
Score = 144 bits (362), Expect(3) = 2e-88
Identities = 64/78 (82%), Positives = 72/78 (92%)
Frame = -3
Query: 683 ALEKDPRALWRRYVEWMYQHRELGLYLDVSRVGFSDEFVAEMEPRFEAAFRAMEELENGA 504
+LEKDPRALW RYVEW+YQH++LGLYLDVSR+GFSDEFV EMEPRFE AF+ ME +E GA
Sbjct: 73 SLEKDPRALWARYVEWLYQHKDLGLYLDVSRIGFSDEFVKEMEPRFEKAFKHMEGIEKGA 132
Query: 503 IANPDEGRMVGHYWLRDS 450
IANPDEGRMVGHYWLR+S
Sbjct: 133 IANPDEGRMVGHYWLRNS 150
Score = 59.7 bits (143), Expect(3) = 2e-88
Identities = 29/44 (65%), Positives = 33/44 (74%)
Frame = -2
Query: 147 RAGGTPETRNGLLEVQKAFREAGLDFPKQGCCYNQEKFFVGYTA 16
++GGTPETRNGLLEVQKAFR+AGL F KQG QE + TA
Sbjct: 252 KSGGTPETRNGLLEVQKAFRDAGLVFAKQGVAITQENSLLDNTA 295
>gb|AAN41353.1| putative glucose-6-phosphate isomerase [Arabidopsis thaliana]
Length = 613
Score = 174 bits (440), Expect(2) = 5e-80
Identities = 91/136 (66%), Positives = 106/136 (77%), Gaps = 3/136 (2%)
Frame = -1
Query: 448 KRAPNEFLKTQIESTLDAVCNFADDVVSGKIK---SPKGRFTQILSVGIGGSALGPQFVA 278
K AP LKT IE+TLD++C F+DD++SGKIK SP+GRFTQILSVGIGGSALGPQFVA
Sbjct: 144 KLAPKPTLKTLIENTLDSICAFSDDIISGKIKPPSSPEGRFTQILSVGIGGSALGPQFVA 203
Query: 277 EALAPDNPPLKIRFIDNTDPAGIDHQIAQLGPELATTLVIVISKSXRYTRD*KWFIRSTE 98
EALAPDNPPLKIRFIDNTDPAGIDHQIAQLGPELA+TLV+VISKS + +
Sbjct: 204 EALAPDNPPLKIRFIDNTDPAGIDHQIAQLGPELASTLVVVISKSGGTPETRNGLLEVQK 263
Query: 97 GLS*SRLGFSETGVLL 50
+ L F++ GV +
Sbjct: 264 AFREAGLNFAKQGVAI 279
Score = 147 bits (370), Expect(2) = 5e-80
Identities = 70/110 (63%), Positives = 86/110 (77%)
Frame = -3
Query: 776 HVSFPARCADRTIRTYAVAREVSDAEKGEIVALEKDPRALWRRYVEWMYQHRELGLYLDV 597
H S P +VAR++S A+ + L KDP ALW+RY++W YQ +ELGLYLD+
Sbjct: 37 HTSKPTNLPLTLSSARSVARDISHADSKK--ELLKDPDALWKRYLDWFYQQKELGLYLDI 94
Query: 596 SRVGFSDEFVAEMEPRFEAAFRAMEELENGAIANPDEGRMVGHYWLRDSK 447
SRVGF+DEFVAEMEPRF+AAF+AME+LE G+IANPDEGRMVGHYWLR+SK
Sbjct: 95 SRVGFTDEFVAEMEPRFQAAFKAMEDLEKGSIANPDEGRMVGHYWLRNSK 144
Score = 62.4 bits (150), Expect = 8e-09
Identities = 30/44 (68%), Positives = 34/44 (77%)
Frame = -2
Query: 147 RAGGTPETRNGLLEVQKAFREAGLDFPKQGCCYNQEKFFVGYTA 16
++GGTPETRNGLLEVQKAFREAGL+F KQG QE + TA
Sbjct: 247 KSGGTPETRNGLLEVQKAFREAGLNFAKQGVAITQENSLLDNTA 290
>ref|NP_194193.1| glucose-6-phosphate isomerase; protein id: At4g24620.1 [Arabidopsis
thaliana] gi|7484979|pir||T05572 glucose-6-phosphate
isomerase (EC 5.3.1.9) - Arabidopsis thaliana
gi|4220528|emb|CAA23001.1| glucose-6-phosphate isomerase
[Arabidopsis thaliana] gi|7269312|emb|CAB79372.1|
glucose-6-phosphate isomerase [Arabidopsis thaliana]
Length = 611
Score = 170 bits (430), Expect(2) = 7e-79
Identities = 89/134 (66%), Positives = 104/134 (77%), Gaps = 1/134 (0%)
Frame = -1
Query: 448 KRAPNEFLKTQIESTLDAVCNFADDVVSGKI-KSPKGRFTQILSVGIGGSALGPQFVAEA 272
K AP LKT IE+TLD++C F+DD++SGK SP+GRFTQILSVGIGGSALGPQFVAEA
Sbjct: 144 KLAPKPTLKTLIENTLDSICAFSDDIISGKPPSSPEGRFTQILSVGIGGSALGPQFVAEA 203
Query: 271 LAPDNPPLKIRFIDNTDPAGIDHQIAQLGPELATTLVIVISKSXRYTRD*KWFIRSTEGL 92
LAPDNPPLKIRFIDNTDPAGIDHQIAQLGPELA+TLV+VISKS + +
Sbjct: 204 LAPDNPPLKIRFIDNTDPAGIDHQIAQLGPELASTLVVVISKSGGTPETRNGLLEVQKAF 263
Query: 91 S*SRLGFSETGVLL 50
+ L F++ GV +
Sbjct: 264 REAGLNFAKQGVAI 277
Score = 147 bits (370), Expect(2) = 7e-79
Identities = 70/110 (63%), Positives = 86/110 (77%)
Frame = -3
Query: 776 HVSFPARCADRTIRTYAVAREVSDAEKGEIVALEKDPRALWRRYVEWMYQHRELGLYLDV 597
H S P +VAR++S A+ + L KDP ALW+RY++W YQ +ELGLYLD+
Sbjct: 37 HTSKPTNLPLTLSSARSVARDISHADSKK--ELLKDPDALWKRYLDWFYQQKELGLYLDI 94
Query: 596 SRVGFSDEFVAEMEPRFEAAFRAMEELENGAIANPDEGRMVGHYWLRDSK 447
SRVGF+DEFVAEMEPRF+AAF+AME+LE G+IANPDEGRMVGHYWLR+SK
Sbjct: 95 SRVGFTDEFVAEMEPRFQAAFKAMEDLEKGSIANPDEGRMVGHYWLRNSK 144
Score = 62.4 bits (150), Expect = 8e-09
Identities = 30/44 (68%), Positives = 34/44 (77%)
Frame = -2
Query: 147 RAGGTPETRNGLLEVQKAFREAGLDFPKQGCCYNQEKFFVGYTA 16
++GGTPETRNGLLEVQKAFREAGL+F KQG QE + TA
Sbjct: 245 KSGGTPETRNGLLEVQKAFREAGLNFAKQGVAITQENSLLDNTA 288
>gb|AAF24124.1|AF120494_1 phosphoglucose isomerase precursor [Arabidopsis thaliana]
Length = 612
Score = 158 bits (399), Expect(2) = 6e-75
Identities = 85/136 (62%), Positives = 102/136 (74%), Gaps = 3/136 (2%)
Frame = -1
Query: 448 KRAPNEFLKTQIESTLDAVCNFADDVVSGKIK---SPKGRFTQILSVGIGGSALGPQFVA 278
K AP LKT IE+TLD++C F+DD++SGKIK SP+GRFTQILSVGIGGSALGPQ ++
Sbjct: 144 KLAPKPTLKTLIENTLDSICAFSDDIISGKIKPPSSPEGRFTQILSVGIGGSALGPQ-LS 202
Query: 277 EALAPDNPPLKIRFIDNTDPAGIDHQIAQLGPELATTLVIVISKSXRYTRD*KWFIRSTE 98
L PDNPPLKIRFIDNTDPAGIDHQIAQLGPELA+TLV+VISKS + +
Sbjct: 203 LRLGPDNPPLKIRFIDNTDPAGIDHQIAQLGPELASTLVVVISKSGGTPETRNGLLEVQK 262
Query: 97 GLS*SRLGFSETGVLL 50
+ L F++ GV +
Sbjct: 263 AFREAGLNFAKQGVAI 278
Score = 145 bits (367), Expect(2) = 6e-75
Identities = 67/94 (71%), Positives = 83/94 (88%)
Frame = -3
Query: 728 AVAREVSDAEKGEIVALEKDPRALWRRYVEWMYQHRELGLYLDVSRVGFSDEFVAEMEPR 549
+VAR++S A+ + L KDP ALW+RY++W YQ +ELGLYLD+SRVGF+DEFVAEMEPR
Sbjct: 53 SVARDISHADSKK--ELLKDPDALWKRYLDWFYQQKELGLYLDISRVGFTDEFVAEMEPR 110
Query: 548 FEAAFRAMEELENGAIANPDEGRMVGHYWLRDSK 447
F+AAF+AME+LE G+IANPDEGRMVGHYWLR+SK
Sbjct: 111 FQAAFKAMEDLEKGSIANPDEGRMVGHYWLRNSK 144
Score = 62.4 bits (150), Expect = 8e-09
Identities = 30/44 (68%), Positives = 34/44 (77%)
Frame = -2
Query: 147 RAGGTPETRNGLLEVQKAFREAGLDFPKQGCCYNQEKFFVGYTA 16
++GGTPETRNGLLEVQKAFREAGL+F KQG QE + TA
Sbjct: 246 KSGGTPETRNGLLEVQKAFREAGLNFAKQGVAITQENSLLDNTA 289
>gb|AAO72628.1| glucose-6-phosphate isomerase-like protein [Oryza sativa (japonica
cultivar-group)]
Length = 346
Score = 172 bits (435), Expect(2) = 2e-73
Identities = 92/135 (68%), Positives = 104/135 (76%), Gaps = 3/135 (2%)
Frame = -1
Query: 451 PKRAPNEFLKTQIESTLDAVCNFADDVVSGKIK---SPKGRFTQILSVGIGGSALGPQFV 281
P APN FL+T+IE+TLD + F+ DVVSGKIK SP GRFTQILS+GIGGSALGPQFV
Sbjct: 75 PGLAPNSFLRTKIETTLDRILAFSQDVVSGKIKPPSSPAGRFTQILSIGIGGSALGPQFV 134
Query: 280 AEALAPDNPPLKIRFIDNTDPAGIDHQIAQLGPELATTLVIVISKSXRYTRD*KWFIRST 101
+EALAPDNPPLKIRFIDNTDPAGIDHQIAQLGPELA+TLVIVISKS +
Sbjct: 135 SEALAPDNPPLKIRFIDNTDPAGIDHQIAQLGPELASTLVIVISKSGGTPETRNGLLEVQ 194
Query: 100 EGLS*SRLGFSETGV 56
+ + L FS+ GV
Sbjct: 195 KAFRDAGLDFSKQGV 209
Score = 127 bits (318), Expect(2) = 2e-73
Identities = 55/74 (74%), Positives = 64/74 (86%)
Frame = -3
Query: 674 KDPRALWRRYVEWMYQHRELGLYLDVSRVGFSDEFVAEMEPRFEAAFRAMEELENGAIAN 495
KDP LW RYVEW+YQH+ELGL++DVSR+GF++EF+ MEPR + AF AM ELE GAIAN
Sbjct: 1 KDPIKLWERYVEWLYQHKELGLFVDVSRMGFTEEFLRRMEPRMQRAFAAMRELEKGAIAN 60
Query: 494 PDEGRMVGHYWLRD 453
PDEGRMVGHYWLRD
Sbjct: 61 PDEGRMVGHYWLRD 74
Score = 63.2 bits (152), Expect = 5e-09
Identities = 30/44 (68%), Positives = 34/44 (77%)
Frame = -2
Query: 147 RAGGTPETRNGLLEVQKAFREAGLDFPKQGCCYNQEKFFVGYTA 16
++GGTPETRNGLLEVQKAFR+AGLDF KQG QE + TA
Sbjct: 179 KSGGTPETRNGLLEVQKAFRDAGLDFSKQGVAVTQENSLLDNTA 222
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 834,583,490
Number of Sequences: 1393205
Number of extensions: 21514483
Number of successful extensions: 136253
Number of sequences better than 10.0: 2297
Number of HSP's better than 10.0 without gapping: 83934
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 116880
length of database: 448,689,247
effective HSP length: 122
effective length of database: 278,718,237
effective search space used: 45152354394
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
