>KMC001315A_C01 KMC001315A_c01
GAAAATGGCAAGATAATCCATATATTAGTGTTTCATAATGTGCTCAAATCTACAAAATTC
ATCCTTCGAGACAAGTGACACTGTACAATTCTATACAATGAAAAAGAACAACCACTGCGT
GGATATGAAATCAAAGAATAAAAGAAGCCATCACAAACTCAACACACTGAAAAAGCAAAC
TAATTAAAATAAAATATGAAAAACCAAAATCAAATGAATGAAATGAAATGAAATACCAAC
AAACACAGCAAAAAATAATTACGATATACATCTTACTACCAAATGAGACACCGCTTTTTC
TGCATGGATTGCCTTCAACAATGACACGAACTTTAAAAGGTCATCTCTGGAGAACCACTG
AAGCTTCAACTTAAGAGAGCCAGGAGCTTTGTTTCCATGATTGGCAGCTACAAGGTTCAT
TCGGATAGAGGGATGGACTTCTGTCAATGGACATGTTAGACCTAAAGTCACACAGCAGCT
GTCACCAACCTCAATCACCATCTCAGTAATCTCAGCAATGGTGCAACATGAATCAATCGT
GAAGTA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001315A_C01 KMC001315A_c01
         (546 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAN05327.1| Unknown protein [Oryza sativa (japonica cultivar-...    52  5e-06
ref|NP_188419.1| unknown protein; protein id: At3g17920.1 [Arabi...    42  0.005
ref|NP_704188.1| hypothetical protein [Plasmodium falciparum 3D7...    34  1.3
gb|AAF05305.1|AF169858_1 spike glycoprotein S-1 subunit [Avian i...    33  1.7
ref|XP_236893.1| similar to Reticulocalbin 1 precursor [Rattus n...    33  1.7

>gb|AAN05327.1| Unknown protein [Oryza sativa (japonica cultivar-group)]
          Length = 1130

 Score = 52.0 bits (123), Expect = 5e-06
 Identities = 37/113 (32%), Positives = 58/113 (50%), Gaps = 18/113 (15%)
 Frame = -1

Query: 546  YFTIDSCCTIAEITEMVIE-----------VGDSC---CVTLGLTCPLTEVHPSIRMNLV 409
            YF++D+CC I +I E++++           V D C   C+TL L     E       N  
Sbjct: 1020 YFSLDACCFINDIQEVLVKLCPTKISCDIIVVDHCDKRCLTLVLDNHAHEGR--FCSNGS 1077

Query: 408  AANHGNKAPGSLKL----KLQWFSRDDLLKFVSLLKAIHAEKAVSHLVVRCIS 262
              N  +K P  +      KL+WFS + ++KF+SLLKA+++  + S L V+C S
Sbjct: 1078 ITNSQSKQPDEIYTVHTWKLKWFSEETVVKFISLLKALYSVSSSSSLPVKCTS 1130

>ref|NP_188419.1| unknown protein; protein id: At3g17920.1 [Arabidopsis thaliana]
          Length = 962

 Score = 42.0 bits (97), Expect = 0.005
 Identities = 26/92 (28%), Positives = 47/92 (50%)
 Frame = -1

Query: 546  YFTIDSCCTIAEITEMVIEVGDSCCVTLGLTCPLTEVHPSIRMNLVAANHGNKAPGSLKL 367
            YF++DS C+I++I EMV+E   S C++L +           + +    +   +   S+  
Sbjct: 876  YFSLDSSCSISDIFEMVVESRGSSCLSLKVK----------QKDSTFQSSQKRTVNSVTW 925

Query: 366  KLQWFSRDDLLKFVSLLKAIHAEKAVSHLVVR 271
            KL+  S + + KFV+L+  +H +     L VR
Sbjct: 926  KLKLLSPECVPKFVALVNGLHPDSTEFPLFVR 957

>ref|NP_704188.1| hypothetical protein [Plasmodium falciparum 3D7]
           gi|23498926|emb|CAD51004.1| hypothetical protein
           [Plasmodium falciparum 3D7]
          Length = 418

 Score = 33.9 bits (76), Expect = 1.3
 Identities = 22/66 (33%), Positives = 32/66 (48%), Gaps = 2/66 (3%)
 Frame = -3

Query: 289 VSFGSKMYIVIIFCCVCWYFISFHSFDFGFS--YFILISLLFQCVEFVMASFIL*FHIHA 116
           V F S  Y ++ F    ++ + F SF +     YFIL+ L F    F  +SFIL     +
Sbjct: 48  VHFISFFYFLVHFISFFYFLVHFISFFYVHHSYYFILLRLSFILFHFFTSSFILFHSFTS 107

Query: 115 VVVLFH 98
             +LFH
Sbjct: 108 SFILFH 113

 Score = 33.5 bits (75), Expect = 1.7
 Identities = 27/81 (33%), Positives = 34/81 (41%), Gaps = 11/81 (13%)
 Frame = -3

Query: 295 SGVSFGSKMYIVIIFCCVCWYFISFHSFDFGFS-----------YFILISLLFQCVEFVM 149
           S + F S     I+F      FI FHSF F              YFIL+ L F    F  
Sbjct: 168 SFILFHSFTSSFILFHSFTSSFILFHSFTFIIHFISFFYVHHSYYFILLRLSFILFHFFT 227

Query: 148 ASFIL*FHIHAVVVLFHCIEL 86
           +SFIL   +    +LFH + L
Sbjct: 228 SSFILFHSLTLSFILFHSLTL 248

>gb|AAF05305.1|AF169858_1 spike glycoprotein S-1 subunit [Avian infectious bronchitis virus]
          Length = 558

 Score = 33.5 bits (75), Expect = 1.7
 Identities = 18/58 (31%), Positives = 28/58 (48%)
 Frame = -1

Query: 546 YFTIDSCCTIAEITEMVIEVGDSCCVTLGLTCPLTEVHPSIRMNLVAANHGNKAPGSL 373
           +FT+  C      T  ++ V   C  +   +CPLT + PS  + + A  HG+  PG L
Sbjct: 90  WFTVSFCTAHCNFTSYIVFV-THCYKSGPNSCPLTGLIPSGYIRIAAMKHGSAMPGHL 146

>ref|XP_236893.1| similar to Reticulocalbin 1 precursor [Rattus norvegicus]
          Length = 393

 Score = 33.5 bits (75), Expect = 1.7
 Identities = 24/100 (24%), Positives = 50/100 (50%), Gaps = 1/100 (1%)
 Frame = +1

Query: 250 KK*LRYTSYYQMRHRFFCMDCLQQ*HEL*KVISGEPLKLQLKRARSFVSMIGSYKVHSDR 429
           K+ ++  S Y++RH     +  Q  + + +++  E  KL +++     S + + ++  + 
Sbjct: 283 KELIQVNSSYRVRHSQLLREQAQLKNNI-QILLNEKRKLLVEQTELPASSVEAKRLCEEA 341

Query: 430 GMDFCQWTC*T*SHTAAVTNLNHHLS-NLSNGAT*INREV 546
           GM+ C  +     H+A+V N +H LS N S+     N E+
Sbjct: 342 GMNICDPSAKQQQHSASVVNQHHFLSVNFSHNGVRRNDEI 381

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 438,195,771
Number of Sequences: 1393205
Number of extensions: 8920650
Number of successful extensions: 23127
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 21813
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22950
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 18660035355
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFBL047f02_f BP043666 1 546 2 MRL001a02_f BP083698 1 536 3 MRL004f06_f BP083912 5 378 4 GENLf074b10 BP066337 7 444 5 GENLf089e03 BP067212 31 547

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