>KMC001304A_C01 KMC001304A_c01
agggggtcttattttGTGACTTAATTCACCTTTTTTGTATTCGTACTGTTACAAAGGATT
TAAGACACTCATTAGGTCCAAATGAAAATTTCGCTCTAATGGTTTGGATCTTACTTTACT
TTTAGTTGGACTCACATTACTAAATATATTGTTTATTTTACATTATCCCAAAGCATTAAT
GCTTCGTCCAGTTGATAAACTTCTTGAGATTGTTTGATGACTGACCACCTTCTGCTAAGT
TATTTATGGTTATTTCCTTCAGTTTCAGAGAGCCTTGCTTTCAAGTCTTCATTAACAATC
ACTTGCTCCACCTTCGTCCTTATCTCTCCCTTTGGTATTATTCCATTTTCATCTTTGTCT
AATCCAAGTCCAATCTTCCACACATCACAAATAAATGACTTGTTAAGAAATTGATCAGTG
GAAAATGGCCAACACAGGAAAGGCACACTAGCATGTACACCTTCTATGGTAGAGTTCCAA
CCACAGTGACTAATGAAGCAAGCTATAGCTGGGTGATTCAATATCTTCTTCTGAGGTCGC
CATTTGACAACTTTACCTTTACTTCCATGGAA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001304A_C01 KMC001304A_c01
         (572 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAG25643.1|AF303396_1 UDP-glucosyltransferase HRA25 [Phaseolu...   149  5e-37
gb|AAK16175.1|AC079887_7 putative glucosyltransferase [Oryza sat...   135  2e-33
ref|NP_186859.1| putative UDP-glucosyl transferase; protein id: ...   126  5e-33
gb|AAM47593.1| putative glucosyl transferase [Sorghum bicolor]        138  6e-32
gb|AAM47590.1| putative glucosyl transferase [Sorghum bicolor]        128  5e-31

>gb|AAG25643.1|AF303396_1 UDP-glucosyltransferase HRA25 [Phaseolus vulgaris]
          Length = 462

 Score =  149 bits (375), Expect(2) = 5e-37
 Identities = 59/100 (59%), Positives = 82/100 (82%)
 Frame = -1

Query: 572 FHGSKGKVVKWRPQKKILNHPAIACFISHCGWNSTIEGVHASVPFLCWPFSTDQFLNKSF 393
           F G KGK+V W PQ+K+L+HPAIACF++HCGWNST+EG+ + VP LCWP+  DQ  NK+ 
Sbjct: 332 FQGHKGKIVNWAPQQKVLSHPAIACFVTHCGWNSTMEGLSSGVPLLCWPYFGDQLYNKAH 391

Query: 392 ICDVWKIGLGLDKDENGIIPKGEIRTKVEQVIVNEDLKAR 273
           ICD  K+GLG+DKD+NG++ +GE++TKVEQ+  +E++K R
Sbjct: 392 ICDELKVGLGIDKDQNGVVSRGELKTKVEQIFNDENIKFR 431

 Score = 27.3 bits (59), Expect(2) = 5e-37
 Identities = 12/27 (44%), Positives = 16/27 (58%)
 Frame = -2

Query: 262 LKEITINNLAEGGQSSNNLKKFINWTK 182
           LKE  + N+A+GG S  N K F+   K
Sbjct: 435 LKEKVMKNIAKGGTSYENFKNFVKEIK 461

>gb|AAK16175.1|AC079887_7 putative glucosyltransferase [Oryza sativa]
          Length = 457

 Score =  135 bits (339), Expect(2) = 2e-33
 Identities = 53/99 (53%), Positives = 78/99 (78%)
 Frame = -1

Query: 560 KGKVVKWRPQKKILNHPAIACFISHCGWNSTIEGVHASVPFLCWPFSTDQFLNKSFICDV 381
           KG +V W PQ+ +L+HP+IACF+SHCGWNST+EG+   VPFLCWP+  DQ+ N+S+IC+V
Sbjct: 330 KGMIVGWAPQQSVLSHPSIACFVSHCGWNSTMEGLRHGVPFLCWPYFADQYCNQSYICNV 389

Query: 380 WKIGLGLDKDENGIIPKGEIRTKVEQVIVNEDLKARLSE 264
           W  G+ L  DE G++ K EI+ KVEQ++ ++++KAR ++
Sbjct: 390 WGTGVKLQADERGVVTKEEIKNKVEQLVDDKEIKARAAK 428

 Score = 28.9 bits (63), Expect(2) = 2e-33
 Identities = 13/30 (43%), Positives = 17/30 (56%)
 Frame = -2

Query: 280 KQGSLKLKEITINNLAEGGQSSNNLKKFIN 191
           K  + K K     ++AEGG S  NL KF+N
Sbjct: 423 KARAAKWKHAACTSIAEGGSSHENLLKFVN 452

>ref|NP_186859.1| putative UDP-glucosyl transferase; protein id: At3g02100.1
           [Arabidopsis thaliana]
           gi|6513946|gb|AAF14850.1|AC011664_32 putative
           UDP-glucosyl transferase [Arabidopsis thaliana]
          Length = 464

 Score =  126 bits (317), Expect(2) = 5e-33
 Identities = 47/86 (54%), Positives = 71/86 (81%)
 Frame = -1

Query: 554 KVVKWRPQKKILNHPAIACFISHCGWNSTIEGVHASVPFLCWPFSTDQFLNKSFICDVWK 375
           KVV+W PQ+++L+  AI CF+SHCGWNST+EG    +PFLC P+  DQF+NK++ICDVWK
Sbjct: 336 KVVRWAPQREVLSSGAIGCFVSHCGWNSTLEGAQNGIPFLCIPYFADQFINKAYICDVWK 395

Query: 374 IGLGLDKDENGIIPKGEIRTKVEQVI 297
           IGLGL++D  G++P+ E++ K+++++
Sbjct: 396 IGLGLERDARGVVPRLEVKKKIDEIM 421

 Score = 36.2 bits (82), Expect(2) = 5e-33
 Identities = 14/33 (42%), Positives = 24/33 (72%)
 Frame = -2

Query: 280 KQGSLKLKEITINNLAEGGQSSNNLKKFINWTK 182
           ++ ++K+KEI + ++A+ G S  NL KF+NW K
Sbjct: 428 EERAMKVKEIVMKSVAKDGISCENLNKFVNWIK 460

>gb|AAM47593.1| putative glucosyl transferase [Sorghum bicolor]
          Length = 457

 Score =  138 bits (347), Expect = 6e-32
 Identities = 56/98 (57%), Positives = 76/98 (77%)
 Frame = -1

Query: 566 GSKGKVVKWRPQKKILNHPAIACFISHCGWNSTIEGVHASVPFLCWPFSTDQFLNKSFIC 387
           G KG VV W PQ+++L+HP++ACFISHCGWNST+EGV   VPFLCWP+  DQF+N+++IC
Sbjct: 328 GDKGLVVGWAPQQRVLSHPSVACFISHCGWNSTMEGVRHGVPFLCWPYFADQFMNQNYIC 387

Query: 386 DVWKIGLGLDKDENGIIPKGEIRTKVEQVIVNEDLKAR 273
           D W  GL +D DE GI  K EIR KV+Q++ ++ ++ R
Sbjct: 388 DAWGTGLRIDADERGIFTKEEIRDKVDQLLGDDGIRTR 425

>gb|AAM47590.1| putative glucosyl transferase [Sorghum bicolor]
          Length = 459

 Score =  128 bits (321), Expect(2) = 5e-31
 Identities = 51/100 (51%), Positives = 77/100 (77%)
 Frame = -1

Query: 566 GSKGKVVKWRPQKKILNHPAIACFISHCGWNSTIEGVHASVPFLCWPFSTDQFLNKSFIC 387
           G +G +V W PQ+++L H A+ACF+SHCGWNST+EGV  +VPFLCWP+ TDQF N+S+IC
Sbjct: 329 GGRGMIVSWCPQQQVLAHRAVACFVSHCGWNSTMEGVRNAVPFLCWPYFTDQFQNESYIC 388

Query: 386 DVWKIGLGLDKDENGIIPKGEIRTKVEQVIVNEDLKARLS 267
           +VW+ GL +    +G++ K E+  KVE+V+ ++ ++ R+S
Sbjct: 389 NVWRTGLAVAPGPDGVVTKEELSGKVERVLGDDGIRERVS 428

 Score = 28.1 bits (61), Expect(2) = 5e-31
 Identities = 11/27 (40%), Positives = 17/27 (62%)
 Frame = -2

Query: 262 LKEITINNLAEGGQSSNNLKKFINWTK 182
           L++    ++AEGG S +N KKF+   K
Sbjct: 430 LRDAACRSIAEGGSSRDNFKKFVELLK 456

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 466,163,021
Number of Sequences: 1393205
Number of extensions: 9583307
Number of successful extensions: 28853
Number of sequences better than 10.0: 700
Number of HSP's better than 10.0 without gapping: 28391
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28847
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 21243732558
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf068a08 BP072383 1 388 2 GENf059h02 BP060875 16 467 3 GENLf073f09 BP066309 40 573 4 GNLf007b09 BP075197 74 562

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