>KMC001281A_C01 KMC001281A_c01
tcaaaataattttattttcagaatatgttagaagtactattctcagtcattctGGGGAGA
TTCCAAACTAATTATATATAGCACCAAAACTTGGGACTTACATAGAATTCAAGGAGAGAT
GCATCTCCCTAATACTCTTAAATTTCCAAGAGAAACCCACAAGAAAGCACATAAAGCAAC
AATAATTGGGAAGAAAAGGAATGAAATTCCCCTCATGCCCGCTCTCTTCTTAGTAGCAGT
GTCTTTACCTGCAGCAATGCTGGTGTTCTTGATCCTCACCCTGCTGAAGCTAGACATTCG
ATTCAAAGCGCGGTTTACCAGAGCGTTTTCAGCAGCTGATGCAGGTGTAGTAGGTATTGA
CTCCACAAAAGCCCAAAGAGCAGAGGAGAATCTACTGGGAGTCATAGGAATATCCTCCAC
AGGGCTGCTGCTTTGATTTTCATTGCCTTCAGGAGGATAGTTGACAGATTCACATACGAA
ACCTATACATAACCAAAAACTAGCATATATTAGGGATAACCTATG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001281A_C01 KMC001281A_c01
         (525 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAM34770.1|AF509870_1 nam-like protein 7 [Petunia x hybrida]       101  5e-21
gb|AAM67294.1| NAM-like protein [Arabidopsis thaliana]                 92  5e-18
pir||A86466 BTF3b factor protein F23M19.14 - Arabidopsis thalian...    87  9e-17
ref|NP_564439.1| expressed protein; protein id: At1g34180.1, sup...    87  9e-17
gb|AAM34769.1|AF509869_1 nam-like protein 6 [Petunia x hybrida]        87  2e-16

>gb|AAM34770.1|AF509870_1 nam-like protein 7 [Petunia x hybrida]
          Length = 603

 Score =  101 bits (252), Expect = 5e-21
 Identities = 58/119 (48%), Positives = 76/119 (63%), Gaps = 3/119 (2%)
 Frame = -1

Query: 462 NYPPEGNENQSSSPVEDIPMTPSRFSSALWAFVESIPTTPASAAENALVNRALNRMSSFS 283
           N+P   N+N  +   +    T S FSS LWAFV+SIPTTPASAAE+ALVNRA  RMSSFS
Sbjct: 487 NHPMGANQNPIAKKDDG---TQSWFSSNLWAFVDSIPTTPASAAESALVNRAFERMSSFS 543

Query: 282 RVRI--KNTSIAAGKDTATKKRAGMRGISFLFFPIIVALCAFLWVSLGN-LRVLGRCIS 115
           RV++  +N ++A G  +AT   +G     F  F ++  LCA LWV +G    ++GR IS
Sbjct: 544 RVKLNARNMNVATGNTSATSSSSGKPRNGFFCFSLLGVLCAILWVLIGTYANIMGRYIS 602

>gb|AAM67294.1| NAM-like protein [Arabidopsis thaliana]
          Length = 564

 Score = 91.7 bits (226), Expect = 5e-18
 Identities = 56/120 (46%), Positives = 72/120 (59%)
 Frame = -1

Query: 483 GFVCESVNYPPEGNENQSSSPVEDIPMTPSRFSSALWAFVESIPTTPASAAENALVNRAL 304
           G + ES+N  P  + N     V++   T S+FSSALW  +ESIP+TPASA E  L N+  
Sbjct: 446 GVMPESMN--PTMSVNPQYKEVQNGGGTRSQFSSALWELLESIPSTPASACEGPL-NQTF 502

Query: 303 NRMSSFSRVRIKNTSIAAGKDTATKKRAGMRGISFLFFPIIVALCAFLWVSLGNLRVLGR 124
            RMSSFSR+R   TS+ + K T  KKR   RG  FL   I+ ALCA  WV +  + V+GR
Sbjct: 503 VRMSSFSRIRFNGTSVTSRKVTVAKKRISNRG--FLLLSIMGALCAIFWVFIATVGVMGR 560

>pir||A86466 BTF3b factor protein F23M19.14 - Arabidopsis thaliana
           gi|5091626|gb|AAD39614.1|AC007454_13 Similar to
           gb|X92204 NAM gene product from Petunia hybrida.
           [Arabidopsis thaliana]
          Length = 576

 Score = 87.4 bits (215), Expect = 9e-17
 Identities = 55/120 (45%), Positives = 70/120 (57%)
 Frame = -1

Query: 483 GFVCESVNYPPEGNENQSSSPVEDIPMTPSRFSSALWAFVESIPTTPASAAENALVNRAL 304
           G + ES+N  P  + N      ++   T S+FSSALW  +ESIP+TPASA E  L N+  
Sbjct: 458 GVMPESMN--PTMSVNPQYKEGQNGGGTRSQFSSALWELLESIPSTPASACEGPL-NQTF 514

Query: 303 NRMSSFSRVRIKNTSIAAGKDTATKKRAGMRGISFLFFPIIVALCAFLWVSLGNLRVLGR 124
            RMSSFSR+R   TS+ + K T  KKR   RG  FL   I+ ALCA  WV    + V+GR
Sbjct: 515 VRMSSFSRIRFNGTSVTSRKVTVAKKRISNRG--FLLLSIMGALCAIFWVFKATVGVMGR 572

>ref|NP_564439.1| expressed protein; protein id: At1g34180.1, supported by cDNA:
           11977. [Arabidopsis thaliana]
          Length = 564

 Score = 87.4 bits (215), Expect = 9e-17
 Identities = 55/120 (45%), Positives = 70/120 (57%)
 Frame = -1

Query: 483 GFVCESVNYPPEGNENQSSSPVEDIPMTPSRFSSALWAFVESIPTTPASAAENALVNRAL 304
           G + ES+N  P  + N      ++   T S+FSSALW  +ESIP+TPASA E  L N+  
Sbjct: 446 GVMPESMN--PTMSVNPQYKEGQNGGGTRSQFSSALWELLESIPSTPASACEGPL-NQTF 502

Query: 303 NRMSSFSRVRIKNTSIAAGKDTATKKRAGMRGISFLFFPIIVALCAFLWVSLGNLRVLGR 124
            RMSSFSR+R   TS+ + K T  KKR   RG  FL   I+ ALCA  WV    + V+GR
Sbjct: 503 VRMSSFSRIRFNGTSVTSRKVTVAKKRISNRG--FLLLSIMGALCAIFWVFKATVGVMGR 560

>gb|AAM34769.1|AF509869_1 nam-like protein 6 [Petunia x hybrida]
          Length = 591

 Score = 86.7 bits (213), Expect = 2e-16
 Identities = 56/126 (44%), Positives = 75/126 (59%), Gaps = 3/126 (2%)
 Frame = -1

Query: 483 GFVCESVNYPPEGNENQSSSPVEDIPMTPSRFSSALWAFVESIPTTPASAAENALVNRAL 304
           G V +S N P   N N+ +   +    TPS  SS LWAFV+SIPTTPA+AAE+ LVN A 
Sbjct: 469 GVVYDS-NLPNHPNHNEIAKQDDG---TPSWLSSQLWAFVDSIPTTPAAAAESPLVNSAF 524

Query: 303 NRMSSFSRVRIK--NTSIAAGKDTATKKRAGMRGISFLFFPIIVALCAFLWVSLGN-LRV 133
            RMSSFSRVRI   N ++ AG  +AT  ++G      ++F  +    A + + +G  + V
Sbjct: 525 KRMSSFSRVRINPGNMNVVAGNSSATSSKSGKSKNGVIYFSFLGIFFAIVCLLVGTFVAV 584

Query: 132 LGRCIS 115
           LGR IS
Sbjct: 585 LGRHIS 590

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 440,084,798
Number of Sequences: 1393205
Number of extensions: 9337629
Number of successful extensions: 22741
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 22141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22702
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17019769648
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENLf071h01 BP066205 1 478 2 MPD035d06_f AV772395 50 525 3 MWM159d07_f AV767190 59 327

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