Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001240A_C01 KMC001240A_c01
(520 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P35100|CLPA_PEA ATP-dependent clp protease ATP-binding subuni... 118 9e-39
sp|P31542|CLAB_LYCES ATP-dependent clp protease ATP-binding subu... 107 9e-29
ref|NP_568746.1| ATP-dependent Clp protease ATP-binding subunit ... 106 2e-28
ref|NP_566912.1| ATP-dependent Clp protease ATP-binding subunit ... 107 5e-25
pir||T52456 endopeptidase Clp ATP-binding chain C [imported] - A... 107 5e-25
>sp|P35100|CLPA_PEA ATP-dependent clp protease ATP-binding subunit clpA homolog,
chloroplast precursor gi|419773|pir||S31164
endopeptidase Clp (EC 3.4.21.-) ATP-binding chain,
chloroplast [similarity] - garden pea
gi|169128|gb|AAA33680.1| nuclear encoded precursor to
chloroplast protein
Length = 922
Score = 118 bits (296), Expect(2) = 9e-39
Identities = 60/73 (82%), Positives = 66/73 (90%)
Frame = +2
Query: 302 GLDFHSKVFATTTMSRKARASRCVPKAMFERFTEKAIKVIMLAQEEARRMGHNFVGTEQV 481
GLDFHSKV + SR+ARA R +P+AMFERFTEKAIKVIMLAQEEARR+GHNFVGTEQ+
Sbjct: 65 GLDFHSKV-SKAVSSRRARAKRFIPRAMFERFTEKAIKVIMLAQEEARRLGHNFVGTEQI 123
Query: 482 LLGLIGEGTGIAA 520
LLGLIGEGTGIAA
Sbjct: 124 LLGLIGEGTGIAA 136
Score = 63.2 bits (152), Expect(2) = 9e-39
Identities = 36/70 (51%), Positives = 47/70 (66%)
Frame = +1
Query: 103 MSRVLAQSINIPGLVAGRTHGRNNRSTKSKRPVKMMYTTLQAPALRLSGFSGLRSFNNFG 282
M+RVLAQS+++PGLVAG ++ S KSKR VK M L+ LR+SGFSGLR+FN+
Sbjct: 1 MARVLAQSLSVPGLVAGHKDSQHKGSGKSKRSVKTM-CALRTSGLRMSGFSGLRTFNHLN 59
Query: 283 YFCLRPWTRF 312
+RP F
Sbjct: 60 TM-MRPGLDF 68
Score = 56.6 bits (135), Expect = 2e-07
Identities = 24/42 (57%), Positives = 36/42 (85%)
Frame = +2
Query: 395 FTEKAIKVIMLAQEEARRMGHNFVGTEQVLLGLIGEGTGIAA 520
FT +A +V+ L+QEEAR++GHN++G+E +LLGL+ EG G+AA
Sbjct: 170 FTPRAKRVLELSQEEARQLGHNYIGSEHLLLGLLREGEGVAA 211
>sp|P31542|CLAB_LYCES ATP-dependent clp protease ATP-binding subunit clpA homolog CD4B,
chloroplast precursor gi|100190|pir||B35905
endopeptidase Clp (EC 3.4.21.-) ATP-binding chain cd4B,
chloroplast [similarity] - tomato
gi|170435|gb|AAA34161.1| ATP-dependent protease (CD4B)
Length = 923
Score = 107 bits (266), Expect(2) = 9e-29
Identities = 60/81 (74%), Positives = 65/81 (80%)
Frame = +2
Query: 278 LDTFV*DLGLDFHSKVFATTTMSRKARASRCVPKAMFERFTEKAIKVIMLAQEEARRMGH 457
+DT V G SKV A T + R+ R R VPKAMFERFTEKAIKVIMLAQEEARR+GH
Sbjct: 58 IDTLVRS-GETLQSKVAAATYV-RRPRGCRFVPKAMFERFTEKAIKVIMLAQEEARRLGH 115
Query: 458 NFVGTEQVLLGLIGEGTGIAA 520
NFVGTEQ+LLGLIGEGTGIAA
Sbjct: 116 NFVGTEQILLGLIGEGTGIAA 136
Score = 53.9 bits (128), Expect = 1e-06
Identities = 23/42 (54%), Positives = 35/42 (82%)
Frame = +2
Query: 395 FTEKAIKVIMLAQEEARRMGHNFVGTEQVLLGLIGEGTGIAA 520
FT +A +V+ L+ EEAR++GHN++G+E +LLGL+ EG G+AA
Sbjct: 170 FTPRAKRVLELSLEEARQLGHNYIGSEHLLLGLLREGEGVAA 211
Score = 41.2 bits (95), Expect(2) = 9e-29
Identities = 25/57 (43%), Positives = 33/57 (57%)
Frame = +1
Query: 103 MSRVLAQSINIPGLVAGRTHGRNNRSTKSKRPVKMMYTTLQAPALRLSGFSGLRSFN 273
M+R L QS +IP VAG + N S K+KR V M+ Q+ +L L F+GLR N
Sbjct: 1 MARALVQSTSIPSSVAGERTTKFNGSGKTKRAVTML-CNAQSSSLTLRDFTGLRGCN 56
>ref|NP_568746.1| ATP-dependent Clp protease ATP-binding subunit (ClpC1); protein id:
At5g50920.1, supported by cDNA: gi_20856955, supported
by cDNA: gi_2921157 [Arabidopsis thaliana]
gi|9758239|dbj|BAB08738.1| ATP-dependent Clp protease,
ATP-binding subunit [Arabidopsis thaliana]
gi|20856956|gb|AAM26692.1| AT5g50920/K3K7_7 [Arabidopsis
thaliana]
Length = 929
Score = 106 bits (264), Expect(2) = 2e-28
Identities = 56/71 (78%), Positives = 60/71 (83%)
Frame = +2
Query: 308 DFHSKVFATTTMSRKARASRCVPKAMFERFTEKAIKVIMLAQEEARRMGHNFVGTEQVLL 487
DFHSKV + K +ASR KAMFERFTEKAIKVIMLAQEEARR+GHNFVGTEQ+LL
Sbjct: 70 DFHSKVRQAMNVP-KGKASRFTVKAMFERFTEKAIKVIMLAQEEARRLGHNFVGTEQILL 128
Query: 488 GLIGEGTGIAA 520
GLIGEGTGIAA
Sbjct: 129 GLIGEGTGIAA 139
Score = 53.9 bits (128), Expect = 1e-06
Identities = 23/42 (54%), Positives = 35/42 (82%)
Frame = +2
Query: 395 FTEKAIKVIMLAQEEARRMGHNFVGTEQVLLGLIGEGTGIAA 520
FT +A +V+ L+ EEAR++GHN++G+E +LLGL+ EG G+AA
Sbjct: 173 FTPRAKRVLELSLEEARQLGHNYIGSEHLLLGLLREGEGVAA 214
Score = 40.8 bits (94), Expect(2) = 2e-28
Identities = 25/56 (44%), Positives = 33/56 (58%)
Frame = +1
Query: 106 SRVLAQSINIPGLVAGRTHGRNNRSTKSKRPVKMMYTTLQAPALRLSGFSGLRSFN 273
+RVLAQS P L + + + S +S+R VKMM + LQ LR+ GF GLR N
Sbjct: 5 TRVLAQSTP-PSLACYQRNVPSRGSGRSRRSVKMMCSQLQVSGLRMQGFMGLRGNN 59
>ref|NP_566912.1| ATP-dependent Clp protease ATP-binding subunit (ClpC2); protein id:
At3g48870.1, supported by cDNA: gi_15983762, supported
by cDNA: gi_5360573 [Arabidopsis thaliana]
gi|11265212|pir||T49283 AtClpC - Arabidopsis thaliana
gi|7576225|emb|CAB87915.1| AtClpC [Arabidopsis thaliana]
Length = 952
Score = 107 bits (267), Expect(2) = 5e-25
Identities = 52/59 (88%), Positives = 57/59 (96%)
Frame = +2
Query: 344 SRKARASRCVPKAMFERFTEKAIKVIMLAQEEARRMGHNFVGTEQVLLGLIGEGTGIAA 520
S + +ASRCVPKAMFERFTEKAIKVIML+QEEARR+GHNFVGTEQ+LLGLIGEGTGIAA
Sbjct: 101 SGREKASRCVPKAMFERFTEKAIKVIMLSQEEARRLGHNFVGTEQILLGLIGEGTGIAA 159
Score = 53.9 bits (128), Expect = 1e-06
Identities = 23/42 (54%), Positives = 35/42 (82%)
Frame = +2
Query: 395 FTEKAIKVIMLAQEEARRMGHNFVGTEQVLLGLIGEGTGIAA 520
FT +A +V+ L+ EEAR++GHN++G+E +LLGL+ EG G+AA
Sbjct: 193 FTPRAKRVLELSLEEARQLGHNYIGSEHLLLGLLREGEGVAA 234
Score = 28.1 bits (61), Expect(2) = 5e-25
Identities = 17/40 (42%), Positives = 26/40 (64%), Gaps = 3/40 (7%)
Frame = +1
Query: 175 RSTKSKRP---VKMMYTTLQAPALRLSGFSGLRSFNNFGY 285
++T+ + P VKMM ++LQAP L + FSGLR+ + Y
Sbjct: 45 QATEYREPRGRVKMM-SSLQAPLLTIQSFSGLRAPSALDY 83
>pir||T52456 endopeptidase Clp ATP-binding chain C [imported] - Arabidopsis
thaliana gi|5360574|dbj|BAA82062.1| AtClpC [Arabidopsis
thaliana]
Length = 952
Score = 107 bits (267), Expect(2) = 5e-25
Identities = 52/59 (88%), Positives = 57/59 (96%)
Frame = +2
Query: 344 SRKARASRCVPKAMFERFTEKAIKVIMLAQEEARRMGHNFVGTEQVLLGLIGEGTGIAA 520
S + +ASRCVPKAMFERFTEKAIKVIML+QEEARR+GHNFVGTEQ+LLGLIGEGTGIAA
Sbjct: 101 SGREKASRCVPKAMFERFTEKAIKVIMLSQEEARRLGHNFVGTEQILLGLIGEGTGIAA 159
Score = 53.9 bits (128), Expect = 1e-06
Identities = 23/42 (54%), Positives = 35/42 (82%)
Frame = +2
Query: 395 FTEKAIKVIMLAQEEARRMGHNFVGTEQVLLGLIGEGTGIAA 520
FT +A +V+ L+ EEAR++GHN++G+E +LLGL+ EG G+AA
Sbjct: 193 FTPRAKRVLELSLEEARQLGHNYIGSEHLLLGLLREGEGVAA 234
Score = 28.1 bits (61), Expect(2) = 5e-25
Identities = 17/40 (42%), Positives = 26/40 (64%), Gaps = 3/40 (7%)
Frame = +1
Query: 175 RSTKSKRP---VKMMYTTLQAPALRLSGFSGLRSFNNFGY 285
++T+ + P VKMM ++LQAP L + FSGLR+ + Y
Sbjct: 45 QATEYREPRGRVKMM-SSLQAPLLTIQSFSGLRAPSALDY 83
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 467,199,247
Number of Sequences: 1393205
Number of extensions: 9854408
Number of successful extensions: 24967
Number of sequences better than 10.0: 108
Number of HSP's better than 10.0 without gapping: 23972
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24955
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 16442828304
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
