Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001216A_C05 KMC001216A_c05
(1570 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|O48905|MDHC_MEDSA Malate dehydrogenase, cytoplasmic gi|743118... 543 e-153
gb|AAG13573.1|AC037425_4 cytoplasmic malate dehydrogenase [Oryza... 513 e-153
emb|CAC10208.1| cytosolic malate dehydrogenase [Cicer arietinum] 543 e-153
gb|AAL11502.1|AF367442_1 NAD-dependent malate dehydrogenase [Pru... 534 e-150
gb|AAO15574.1|AF459645_1 malate dehydrogenase [Lupinus albus] 534 e-150
>sp|O48905|MDHC_MEDSA Malate dehydrogenase, cytoplasmic gi|7431180|pir||T09291 malate
dehydrogenase (EC 1.1.1.37), cytosolic - alfalfa
gi|2827082|gb|AAB99756.1| malate dehydrogenase [Medicago
sativa]
Length = 332
Score = 543 bits (1400), Expect = e-153
Identities = 283/320 (88%), Positives = 293/320 (91%)
Frame = -1
Query: 1516 MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVD 1337
MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDI PAAESLNGVKMELVD
Sbjct: 1 MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIAPAAESLNGVKMELVD 60
Query: 1336 AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 1157
AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVM+KNVSIYKSQASALEKH
Sbjct: 61 AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKH 120
Query: 1156 AAANCKVLVVANPANTNALILKEFAPSIPERNISCLTRLDHNRALGQISERLSVPVSDVK 977
AAANCKVLVVANPANTNALILKEFAPSIPERNISCLTRLDHNRALGQISERL+V VSDVK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPERNISCLTRLDHNRALGQISERLNVQVSDVK 180
Query: 976 NVIIWGNHSSTQYPDVNHATVKTPAGEKPVRELVSDDTWLNGEFITTVQQRGAAIIKARK 797
NVIIWGNHSSTQYPDVNHATV TPAGEKPVR+LVSDD WLNGEFI+TVQQRGAAIIKARK
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVNTPAGEKPVRQLVSDDAWLNGEFISTVQQRGAAIIKARK 240
Query: 796 LSSALSAASGACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPSWTDLFIPCDPVPMREWKI 617
LSSALSAAS ACDHIRDWVLGTP+GT+VSMGVYSDGSYNVPS P EWKI
Sbjct: 241 LSSALSAASAACDHIRDWVLGTPQGTFVSMGVYSDGSYNVPSGLIYSFPV-TCANGEWKI 299
Query: 616 VPRTFN**VFQGKSVDLTAD 557
V + + F K +DLTA+
Sbjct: 300 V-QGLSIDEFSRKKLDLTAE 318
Score = 54.7 bits (130), Expect = 4e-06
Identities = 34/57 (59%), Positives = 39/57 (67%), Gaps = 2/57 (3%)
Frame = -2
Query: 675 PAGLIYSFPVTLCPCENG--K*FQGLSIDEFSKEKVWT*QQTRRLAEEKALAYSCLS 511
P+GLIYSFPVT C NG K QGLSIDEFS++K+ L EEK LA+SCLS
Sbjct: 281 PSGLIYSFPVT---CANGEWKIVQGLSIDEFSRKKLDL--TAEELTEEKNLAHSCLS 332
>gb|AAG13573.1|AC037425_4 cytoplasmic malate dehydrogenase [Oryza sativa]
gi|13445906|gb|AAK26431.1|AF353203_1 cytoplasmic malate
dehydrogenase [Oryza sativa]
Length = 332
Score = 513 bits (1322), Expect(2) = e-153
Identities = 255/282 (90%), Positives = 271/282 (95%)
Frame = -1
Query: 1516 MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVD 1337
MAK+P+RVLVTGAAGQIGYALVPMIARGVMLG DQPVILHMLDIPPA ESLNG+KMELVD
Sbjct: 1 MAKEPMRVLVTGAAGQIGYALVPMIARGVMLGADQPVILHMLDIPPATESLNGLKMELVD 60
Query: 1336 AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 1157
AAFPLLKG+VATTDVVEACTGVN+AVMVGGFPRKEGMERKDVM+KNVSIYKSQASALE H
Sbjct: 61 AAFPLLKGIVATTDVVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEAH 120
Query: 1156 AAANCKVLVVANPANTNALILKEFAPSIPERNISCLTRLDHNRALGQISERLSVPVSDVK 977
AA NCKVLVVANPANTNALILKEFAPSIPE+NI+CLTRLDHNRALGQISE+L+V V+DVK
Sbjct: 121 AAPNCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISEKLNVQVTDVK 180
Query: 976 NVIIWGNHSSTQYPDVNHATVKTPAGEKPVRELVSDDTWLNGEFITTVQQRGAAIIKARK 797
N IIWGNHSSTQYPDVNHATVKTP+GEKPVRELV+DD WLN EFI+TVQQRGAAIIKARK
Sbjct: 181 NAIIWGNHSSTQYPDVNHATVKTPSGEKPVRELVADDEWLNTEFISTVQQRGAAIIKARK 240
Query: 796 LSSALSAASGACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPS 671
SSALSAAS ACDHIRDWVLGTPEGT+VSMGVYSDGSY VP+
Sbjct: 241 QSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYGVPA 282
Score = 53.1 bits (126), Expect(2) = e-153
Identities = 32/57 (56%), Positives = 39/57 (68%), Gaps = 2/57 (3%)
Frame = -2
Query: 675 PAGLIYSFPVTLCPCENGK--*FQGLSIDEFSKEKVWT*QQTRRLAEEKALAYSCLS 511
PAGLIYSFPVT C G+ QGL IDEFS++K+ + L+EEK LAYSCL+
Sbjct: 281 PAGLIYSFPVT---CSGGEWTIVQGLPIDEFSRKKMDA--TAQELSEEKTLAYSCLN 332
>emb|CAC10208.1| cytosolic malate dehydrogenase [Cicer arietinum]
Length = 332
Score = 543 bits (1398), Expect = e-153
Identities = 282/320 (88%), Positives = 293/320 (91%)
Frame = -1
Query: 1516 MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVD 1337
MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVD
Sbjct: 1 MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
Query: 1336 AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 1157
AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVM+KNVSIYKSQASALE+H
Sbjct: 61 AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEQH 120
Query: 1156 AAANCKVLVVANPANTNALILKEFAPSIPERNISCLTRLDHNRALGQISERLSVPVSDVK 977
AAANCKVLVVANPANTNALILKEFAPSIPE+NISCLTRLDHNRALGQISERLSV VS++K
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLSVQVSEIK 180
Query: 976 NVIIWGNHSSTQYPDVNHATVKTPAGEKPVRELVSDDTWLNGEFITTVQQRGAAIIKARK 797
NVIIWGNHSSTQYPDV HATVKTPAGEKPVRELVSDD WLNGEFI TVQQRGAAIIKARK
Sbjct: 181 NVIIWGNHSSTQYPDVTHATVKTPAGEKPVRELVSDDAWLNGEFIPTVQQRGAAIIKARK 240
Query: 796 LSSALSAASGACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPSWTDLFIPCDPVPMREWKI 617
LSSALSAAS ACDHIRDWVLGTPEGT+VSMGVYSDGSYNVP+ P EWKI
Sbjct: 241 LSSALSAASAACDHIRDWVLGTPEGTFVSMGVYSDGSYNVPAGLIYSFPV-TCAGGEWKI 299
Query: 616 VPRTFN**VFQGKSVDLTAD 557
V + + F K +DLTA+
Sbjct: 300 V-QGLSIDEFSRKKLDLTAE 318
Score = 56.6 bits (135), Expect = 1e-06
Identities = 34/55 (61%), Positives = 38/55 (68%)
Frame = -2
Query: 675 PAGLIYSFPVTLCPCENGK*FQGLSIDEFSKEKVWT*QQTRRLAEEKALAYSCLS 511
PAGLIYSFPVT C K QGLSIDEFS++K+ L+EEK LAYSCLS
Sbjct: 281 PAGLIYSFPVT-CAGGEWKIVQGLSIDEFSRKKLDL--TAEELSEEKNLAYSCLS 332
>gb|AAL11502.1|AF367442_1 NAD-dependent malate dehydrogenase [Prunus persica]
Length = 332
Score = 534 bits (1375), Expect = e-150
Identities = 276/320 (86%), Positives = 289/320 (90%)
Frame = -1
Query: 1516 MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVD 1337
MAK PVRVLVTGAAGQIGYALVPMIARGVMLG DQPVILH+LDIPPAAE+LNGVKMELVD
Sbjct: 1 MAKGPVRVLVTGAAGQIGYALVPMIARGVMLGADQPVILHLLDIPPAAEALNGVKMELVD 60
Query: 1336 AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 1157
AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVM+KNVSIYKSQASALEKH
Sbjct: 61 AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKH 120
Query: 1156 AAANCKVLVVANPANTNALILKEFAPSIPERNISCLTRLDHNRALGQISERLSVPVSDVK 977
AAANCKVLVVANPANTNALILKEFAPSIPE+NI+CLTRLDHNRALGQ+SERL+V VSDVK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQVSERLNVQVSDVK 180
Query: 976 NVIIWGNHSSTQYPDVNHATVKTPAGEKPVRELVSDDTWLNGEFITTVQQRGAAIIKARK 797
NVIIWGNHSS+QYPDVNHATVKTP+GEK VRELV+DD WL GEFITTVQQRGAAIIKARK
Sbjct: 181 NVIIWGNHSSSQYPDVNHATVKTPSGEKAVRELVADDAWLTGEFITTVQQRGAAIIKARK 240
Query: 796 LSSALSAASGACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPSWTDLFIPCDPVPMREWKI 617
LS ALSAAS ACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPS P EWKI
Sbjct: 241 LSRALSAASSACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPSGLIYSFPV-TCQNGEWKI 299
Query: 616 VPRTFN**VFQGKSVDLTAD 557
V + + F K +D TAD
Sbjct: 300 V-QGLSIDEFSRKKLDATAD 318
Score = 60.1 bits (144), Expect = 1e-07
Identities = 36/57 (63%), Positives = 42/57 (73%), Gaps = 2/57 (3%)
Frame = -2
Query: 675 PAGLIYSFPVTLCPCENG--K*FQGLSIDEFSKEKVWT*QQTRRLAEEKALAYSCLS 511
P+GLIYSFPVT C+NG K QGLSIDEFS++K+ L+EEKALAYSCLS
Sbjct: 281 PSGLIYSFPVT---CQNGEWKIVQGLSIDEFSRKKLDA--TADELSEEKALAYSCLS 332
>gb|AAO15574.1|AF459645_1 malate dehydrogenase [Lupinus albus]
Length = 332
Score = 534 bits (1375), Expect = e-150
Identities = 277/320 (86%), Positives = 288/320 (89%)
Frame = -1
Query: 1516 MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVD 1337
MAK+PVRVLVTGAAGQIGYALVPMIARGVMLG DQPVILH+LDIPPAAESLNGVKMELVD
Sbjct: 1 MAKNPVRVLVTGAAGQIGYALVPMIARGVMLGSDQPVILHLLDIPPAAESLNGVKMELVD 60
Query: 1336 AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 1157
AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVM+KNVSIYKSQA ALEKH
Sbjct: 61 AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQAFALEKH 120
Query: 1156 AAANCKVLVVANPANTNALILKEFAPSIPERNISCLTRLDHNRALGQISERLSVPVSDVK 977
AAANCKVLVVANPANTNALILKEFAPSIPE+NISCLTRLDHNRALGQISERL+V VSDVK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
Query: 976 NVIIWGNHSSTQYPDVNHATVKTPAGEKPVRELVSDDTWLNGEFITTVQQRGAAIIKARK 797
NVIIWGNHSS+QYPDVNHATV TPAGEKPVRELV+DD WLN EFI TVQQRGAAIIKARK
Sbjct: 181 NVIIWGNHSSSQYPDVNHATVTTPAGEKPVRELVADDAWLNSEFIATVQQRGAAIIKARK 240
Query: 796 LSSALSAASGACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPSWTDLFIPCDPVPMREWKI 617
LSSALSAAS ACDHIRDWVLGTPEG WVSMGVYSDGSYNVP+ P EWKI
Sbjct: 241 LSSALSAASAACDHIRDWVLGTPEGIWVSMGVYSDGSYNVPAGLIYSFPV-TTQNGEWKI 299
Query: 616 VPRTFN**VFQGKSVDLTAD 557
V + F K +DLTA+
Sbjct: 300 V-QGLGIDEFSRKKLDLTAE 318
Score = 55.1 bits (131), Expect = 3e-06
Identities = 34/57 (59%), Positives = 40/57 (69%), Gaps = 2/57 (3%)
Frame = -2
Query: 675 PAGLIYSFPVTLCPCENG--K*FQGLSIDEFSKEKVWT*QQTRRLAEEKALAYSCLS 511
PAGLIYSFPVT +NG K QGL IDEFS++K+ L+EEKALAYSCL+
Sbjct: 281 PAGLIYSFPVTT---QNGEWKIVQGLGIDEFSRKKLDL--TAEELSEEKALAYSCLT 332
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,389,447,094
Number of Sequences: 1393205
Number of extensions: 32600608
Number of successful extensions: 88907
Number of sequences better than 10.0: 462
Number of HSP's better than 10.0 without gapping: 82205
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 88469
length of database: 448,689,247
effective HSP length: 128
effective length of database: 270,359,007
effective search space used: 106521448758
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
