Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001196A_C01 KMC001196A_c01
(1058 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAC26045.1| aconitase-iron regulated protein 1 [Citrus limon] 290 e-117
gb|AAM97080.1| cytoplasmic aconitate hydratase [Arabidopsis thal... 346 4e-94
ref|NP_178634.1| cytoplasmic aconitate hydratase; protein id: At... 311 1e-83
sp|P49608|ACOC_CUCMA Aconitate hydratase, cytoplasmic (Citrate h... 309 4e-83
gb|AAK91447.1| AT4g26970/F10M23_310 [Arabidopsis thaliana] gi|23... 306 4e-82
>gb|AAC26045.1| aconitase-iron regulated protein 1 [Citrus limon]
Length = 898
Score = 290 bits (741), Expect(2) = e-117
Identities = 143/164 (87%), Positives = 150/164 (91%)
Frame = +1
Query: 337 MASENPFKGNLTSLPKPGGGEFGKFYSLPSLNDPRVEKLPYSIRILLESAIRNCDNFQVT 516
MA EN FKG LTSLPKPGGGEFGKF+SLP+LNDPR+++LPYSIRILLESAIRNCDNFQVT
Sbjct: 1 MAPENAFKGILTSLPKPGGGEFGKFFSLPALNDPRIDRLPYSIRILLESAIRNCDNFQVT 60
Query: 517 KEDVEKIIDWEKTSSKQVEIPLKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINP 696
K+DVEKIIDWE TS KQVEIP KPARVLLQDFTGVPA VDLACMRDAM L SD KINP
Sbjct: 61 KDDVEKIIDWENTSPKQVEIPFKPARVLLQDFTGVPAAVDLACMRDAMKNLNSDPKKINP 120
Query: 697 LVPVDLVVDHSVQVDVARSENAVQANMELEFQRNKERFAFLKMG 828
LVPVDLVVDHSVQVDVARSENAVQANME EFQRN+ERFAFLK G
Sbjct: 121 LVPVDLVVDHSVQVDVARSENAVQANMEFEFQRNQERFAFLKWG 164
Score = 155 bits (393), Expect(2) = e-117
Identities = 73/81 (90%), Positives = 77/81 (94%)
Frame = +2
Query: 815 FLRWGSTAFHNMLVVPPGSGIVHQVNLEYLGRVVFNNEGLLYPDSVVGTDSHTTMIDGLG 994
FL+WGS+AFHNM VVPPGSGIVHQVNLEYLGRVVFN +G+LYPDSV GTDSHTTMIDGLG
Sbjct: 160 FLKWGSSAFHNMPVVPPGSGIVHQVNLEYLGRVVFNTDGILYPDSVGGTDSHTTMIDGLG 219
Query: 995 VAGWGVGGIEAEAEMLGQPMS 1057
VAGWGVGGIEAEA MLGQPMS
Sbjct: 220 VAGWGVGGIEAEAAMLGQPMS 240
>gb|AAM97080.1| cytoplasmic aconitate hydratase [Arabidopsis thaliana]
Length = 990
Score = 346 bits (887), Expect = 4e-94
Identities = 189/259 (72%), Positives = 207/259 (78%), Gaps = 15/259 (5%)
Frame = +1
Query: 97 MYITTASS----LLRTTRPRLFFSSRIFAH----PPPRASFSPAP---SRSFISSLP--- 234
MY+T +SS ++R R SS +F+ P +S SP+ RSF + P
Sbjct: 1 MYLTASSSASSSIIRAASSR---SSSLFSFRSVLSPSVSSTSPSSLLARRSFGTISPAFR 57
Query: 235 RWSHRLHCG-SPSTLRPQIRAVAPVLERFHRKIATMASENPFKGNLTSLPKPGGGEFGKF 411
RWSH H SP QIRAV+PVL+R R ++MASE+PFKG T+LPKPGGGEFGKF
Sbjct: 58 RWSHSFHSKPSPFRFTSQIRAVSPVLDRLQRTFSSMASEHPFKGIFTTLPKPGGGEFGKF 117
Query: 412 YSLPSLNDPRVEKLPYSIRILLESAIRNCDNFQVTKEDVEKIIDWEKTSSKQVEIPLKPA 591
YSLP+LNDPRV+KLPYSIRILLESAIRNCDNFQVTKEDVEKIIDWEKTS KQVEIP KPA
Sbjct: 118 YSLPALNDPRVDKLPYSIRILLESAIRNCDNFQVTKEDVEKIIDWEKTSPKQVEIPFKPA 177
Query: 592 RVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVVDHSVQVDVARSENAVQA 771
RVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLV+DHSVQVDVARSENAVQA
Sbjct: 178 RVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVIDHSVQVDVARSENAVQA 237
Query: 772 NMELEFQRNKERFAFLKMG 828
NMELEFQRNKERFAFLK G
Sbjct: 238 NMELEFQRNKERFAFLKWG 256
Score = 160 bits (406), Expect = 2e-38
Identities = 77/92 (83%), Positives = 81/92 (87%)
Frame = +2
Query: 782 LSSRETRRDLLFLRWGSTAFHNMLVVPPGSGIVHQVNLEYLGRVVFNNEGLLYPDSVVGT 961
L + + FL+WGSTAF NMLVVPPGSGIVHQVNLEYLGRVVFN +GLLYPDSVVGT
Sbjct: 241 LEFQRNKERFAFLKWGSTAFQNMLVVPPGSGIVHQVNLEYLGRVVFNTKGLLYPDSVVGT 300
Query: 962 DSHTTMIDGLGVAGWGVGGIEAEAEMLGQPMS 1057
DSHTTMIDGLGVAGWGVGGIEAEA MLGQPMS
Sbjct: 301 DSHTTMIDGLGVAGWGVGGIEAEATMLGQPMS 332
>ref|NP_178634.1| cytoplasmic aconitate hydratase; protein id: At2g05710.1
[Arabidopsis thaliana] gi|25291948|pir||B84471
cytoplasmic aconitate hydratase [imported] - Arabidopsis
thaliana gi|4586021|gb|AAD25640.1| cytoplasmic aconitate
hydratase [Arabidopsis thaliana]
Length = 898
Score = 311 bits (797), Expect = 1e-83
Identities = 154/164 (93%), Positives = 159/164 (96%)
Frame = +1
Query: 337 MASENPFKGNLTSLPKPGGGEFGKFYSLPSLNDPRVEKLPYSIRILLESAIRNCDNFQVT 516
MASE+PFKG T+LPKPGGGEFGKFYSLP+LNDPRV+KLPYSIRILLESAIRNCDNFQVT
Sbjct: 1 MASEHPFKGIFTTLPKPGGGEFGKFYSLPALNDPRVDKLPYSIRILLESAIRNCDNFQVT 60
Query: 517 KEDVEKIIDWEKTSSKQVEIPLKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINP 696
KEDVEKIIDWEKTS KQVEIP KPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINP
Sbjct: 61 KEDVEKIIDWEKTSPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINP 120
Query: 697 LVPVDLVVDHSVQVDVARSENAVQANMELEFQRNKERFAFLKMG 828
LVPVDLV+DHSVQVDVARSENAVQANMELEFQRNKERFAFLK G
Sbjct: 121 LVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFAFLKWG 164
Score = 160 bits (406), Expect = 2e-38
Identities = 77/92 (83%), Positives = 81/92 (87%)
Frame = +2
Query: 782 LSSRETRRDLLFLRWGSTAFHNMLVVPPGSGIVHQVNLEYLGRVVFNNEGLLYPDSVVGT 961
L + + FL+WGSTAF NMLVVPPGSGIVHQVNLEYLGRVVFN +GLLYPDSVVGT
Sbjct: 149 LEFQRNKERFAFLKWGSTAFQNMLVVPPGSGIVHQVNLEYLGRVVFNTKGLLYPDSVVGT 208
Query: 962 DSHTTMIDGLGVAGWGVGGIEAEAEMLGQPMS 1057
DSHTTMIDGLGVAGWGVGGIEAEA MLGQPMS
Sbjct: 209 DSHTTMIDGLGVAGWGVGGIEAEATMLGQPMS 240
>sp|P49608|ACOC_CUCMA Aconitate hydratase, cytoplasmic (Citrate hydro-lyase) (Aconitase)
gi|7437043|pir||T10101 aconitate hydratase (EC 4.2.1.3)
- cucurbit gi|868003|dbj|BAA06108.1| aconitase
[Cucurbita cv. Kurokawa Amakuri]
Length = 898
Score = 309 bits (792), Expect = 4e-83
Identities = 152/166 (91%), Positives = 159/166 (95%)
Frame = +1
Query: 337 MASENPFKGNLTSLPKPGGGEFGKFYSLPSLNDPRVEKLPYSIRILLESAIRNCDNFQVT 516
MA+ENPFK NLTSLPKPGGGEFGK+YSLPSLNDPR+++LPYSIRILLESAIRNCDNFQV
Sbjct: 1 MAAENPFKENLTSLPKPGGGEFGKYYSLPSLNDPRIDRLPYSIRILLESAIRNCDNFQVK 60
Query: 517 KEDVEKIIDWEKTSSKQVEIPLKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINP 696
KEDVEKIIDWE +S KQVEIP KPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINP
Sbjct: 61 KEDVEKIIDWENSSPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNKLGSDSNKINP 120
Query: 697 LVPVDLVVDHSVQVDVARSENAVQANMELEFQRNKERFAFLKMGIN 834
LVPVDLV+DHSVQVDVARSENAVQANMELEFQRNKERFAFLK G N
Sbjct: 121 LVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFAFLKWGSN 166
Score = 158 bits (400), Expect = 1e-37
Identities = 76/92 (82%), Positives = 79/92 (85%)
Frame = +2
Query: 782 LSSRETRRDLLFLRWGSTAFHNMLVVPPGSGIVHQVNLEYLGRVVFNNEGLLYPDSVVGT 961
L + + FL+WGS AF NMLVVPPGSGIVHQVNLEYLGRVVFN GLLYPDSVVGT
Sbjct: 149 LEFQRNKERFAFLKWGSNAFQNMLVVPPGSGIVHQVNLEYLGRVVFNTSGLLYPDSVVGT 208
Query: 962 DSHTTMIDGLGVAGWGVGGIEAEAEMLGQPMS 1057
DSHTTMIDGLGVAGWGVGGIEAEA MLGQPMS
Sbjct: 209 DSHTTMIDGLGVAGWGVGGIEAEAAMLGQPMS 240
>gb|AAK91447.1| AT4g26970/F10M23_310 [Arabidopsis thaliana]
gi|23308183|gb|AAN18061.1| At4g26970/F10M23_310
[Arabidopsis thaliana]
Length = 995
Score = 306 bits (784), Expect = 4e-82
Identities = 159/256 (62%), Positives = 193/256 (75%), Gaps = 11/256 (4%)
Frame = +1
Query: 94 TMYITTASSLLRTTRPRLFFSSRIFAHPPPRASFSPAPSRS--FISSL---------PRW 240
T + +AS+ L ++ R+ S P +S +RS F S+L RW
Sbjct: 6 TSGVRSASARLSSSLSRIASSETASVSAPSASSLRNQTNRSKSFSSALRSFRVCSASTRW 65
Query: 241 SHRLHCGSPSTLRPQIRAVAPVLERFHRKIATMASENPFKGNLTSLPKPGGGEFGKFYSL 420
SH GSP++LR Q R PV+E+F RK ATMASE+ +K LTSLPKPGGGE+GK+YSL
Sbjct: 66 SHGGSWGSPASLRAQARNSTPVMEKFERKYATMASEHSYKDILTSLPKPGGGEYGKYYSL 125
Query: 421 PSLNDPRVEKLPYSIRILLESAIRNCDNFQVTKEDVEKIIDWEKTSSKQVEIPLKPARVL 600
P+LNDPR++KLP+S+RILLESAIRNCDN+QVTK+DVEKI+DWE TS+KQVEI KPARV+
Sbjct: 126 PALNDPRIDKLPFSVRILLESAIRNCDNYQVTKDDVEKILDWENTSTKQVEIAFKPARVI 185
Query: 601 LQDFTGVPAVVDLACMRDAMNKLGSDSNKINPLVPVDLVVDHSVQVDVARSENAVQANME 780
LQDFTGVP +VDLA MRDA+ LGSD +KINPLVPVDLVVDHS+QVD ARSE+A Q N+E
Sbjct: 186 LQDFTGVPVLVDLASMRDAVKNLGSDPSKINPLVPVDLVVDHSIQVDFARSEDAAQKNLE 245
Query: 781 LEFQRNKERFAFLKMG 828
LEF+RNKERF FLK G
Sbjct: 246 LEFKRNKERFTFLKWG 261
Score = 160 bits (404), Expect = 4e-38
Identities = 76/92 (82%), Positives = 81/92 (87%)
Frame = +2
Query: 782 LSSRETRRDLLFLRWGSTAFHNMLVVPPGSGIVHQVNLEYLGRVVFNNEGLLYPDSVVGT 961
L + + FL+WGSTAF NMLVVPPGSGIVHQVNLEYLGRVVFN++G LYPDSVVGT
Sbjct: 246 LEFKRNKERFTFLKWGSTAFQNMLVVPPGSGIVHQVNLEYLGRVVFNSKGFLYPDSVVGT 305
Query: 962 DSHTTMIDGLGVAGWGVGGIEAEAEMLGQPMS 1057
DSHTTMIDGLGVAGWGVGGIEAEA MLGQPMS
Sbjct: 306 DSHTTMIDGLGVAGWGVGGIEAEAAMLGQPMS 337
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 975,721,280
Number of Sequences: 1393205
Number of extensions: 23775318
Number of successful extensions: 144478
Number of sequences better than 10.0: 363
Number of HSP's better than 10.0 without gapping: 103052
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139327
length of database: 448,689,247
effective HSP length: 124
effective length of database: 275,931,827
effective search space used: 62912456556
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
