>KMC001173A_C04 KMC001173A_c04
actcaaaacggaaatcatgtatattagatcttaaatggataaaaagtgatatgagatcat
ccactccgtgccgcagtgccCGACACCACTTTTACTCTCACTCTCACTCTCACTCTCACT
CTCAAACCTGAGGAGGAGGAGGAGGAAGAGGAATGAGAGGAGAAAGGGAGAGAAGGGGCT
GAATCAGACGAAATAAATTTTGGAAACACCTACATTTGACTAAGATAGCCACATGGGCCG
GTGGCTGAGCAAAAAATAGATCACATGCTCTTAAAATTTTTGGAAGCAACGAAAGGGGCT
CAACATTTCCAGGAAAGCAAAATAGAAAGGAAGACATTACAAACCAGCAACTTGTCTATG
GGGAGTGGATGCAGCAAGTAGCAGGAAAATAAAAGGAAGTCTGTAAAGACAAATGCAGCC
CAGCAATATTAAATTTAACTACGATATTTCCAAGCTAAGTCTGTAAAACTGATGCACTTT
CAGTGACTAGATTGGATACGAAATCTGGAATGCGAGTAATAACCAGCCCGAATTTATCAG
GTTTGTTCCTTGATGGATCAGCTTCGATCGCTTCTAAATCTGATGCCAGTATCCCAACAA
GATTGAAGAACATTTCATGTGGACTAGTTGCAACACAGCAAATTGAGGGTTTACCATCAC
AAAGAATAACTCTGTTGTCAAGAAGGAGAAATGCCATGTAGGCAGAGGACTCAGCCACAA
CAGCGGCTTTCTTTGTATGAAGAAAAAAGCTCTTAATGACATCGGCAACATCATCAAAGT
CATTAAAATGAGGAAGATATTGACTTGGCTCATCTATGAGATAGATATCTGCAGGCTTAA
AGAGACAGAGACATATCTCAAGTCT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001173A_C04 KMC001173A_c04
         (865 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAM19067.1| RNase L inhibitor-like protein [Oryza sativa (jap...    79  1e-13
gb|AAL78685.1| RNaseL inhibitor-like protein ABCE.1 [Dictyosteli...    76  6e-13
ref|NP_187973.1| RNase L inhibitor protein, putative; protein id...    75  1e-12
ref|NP_193656.2| RNase L inhibitor protein, putative; protein id...    75  2e-12
pir||T04442 ABC-type transport protein T18B16.180 - Arabidopsis ...    75  2e-12

>gb|AAM19067.1| RNase L inhibitor-like protein [Oryza sativa (japonica
           cultivar-group)]
          Length = 604

 Score = 78.6 bits (192), Expect = 1e-13
 Identities = 47/109 (43%), Positives = 59/109 (54%)
 Frame = -1

Query: 865 RLEICLCLFKPADIYLIDEPSQYLPHFNDFDDVADVIKSFFLHTKKAAVVAESSAYMAFL 686
           R+ ICLCL KPADIYLIDEPS YL         + VIK F LH KK A + E    MA  
Sbjct: 474 RVAICLCLGKPADIYLIDEPSAYLDSEQRI-VASKVIKRFILHAKKTAFIVEHDFIMATY 532

Query: 685 LLDNRVILCDGKPSICCVATSPHEMFFNLVGILASDLEAIEADPSRNKP 539
           L D +VI+ +G+PSI C A +P  +   +   L+        DP   +P
Sbjct: 533 LAD-KVIVYEGRPSIDCTANAPQSLVSGMNKFLSHLDITFRRDPPNYRP 580

>gb|AAL78685.1| RNaseL inhibitor-like protein ABCE.1 [Dictyostelium discoideum]
          Length = 603

 Score = 76.3 bits (186), Expect = 6e-13
 Identities = 47/109 (43%), Positives = 58/109 (53%)
 Frame = -1

Query: 865 RLEICLCLFKPADIYLIDEPSQYLPHFNDFDDVADVIKSFFLHTKKAAVVAESSAYMAFL 686
           R+ I LCL +PADIYLIDEPS YL        V+ VIK F LH  K+  + E    MA  
Sbjct: 470 RVAIVLCLGQPADIYLIDEPSAYLDSEQRI-IVSKVIKRFILHAGKSGFIVEHDFIMATY 528

Query: 685 LLDNRVILCDGKPSICCVATSPHEMFFNLVGILASDLEAIEADPSRNKP 539
           L D RVI+ DG P + CVA +P  +   +   L S       DPS  +P
Sbjct: 529 LAD-RVIVYDGIPGVSCVANTPQSLLTGMNSFLKSLEITFRRDPSNFRP 576

>ref|NP_187973.1| RNase L inhibitor protein, putative; protein id: At3g13640.1
           [Arabidopsis thaliana] gi|9294008|dbj|BAB01911.1| RNase
           L inhibitor [Arabidopsis thaliana]
           gi|28393118|gb|AAO41992.1| putative RNase L inhibitor
           protein [Arabidopsis thaliana]
           gi|28827462|gb|AAO50575.1| putative RNase L inhibitor
           protein [Arabidopsis thaliana]
          Length = 603

 Score = 75.5 bits (184), Expect = 1e-12
 Identities = 45/109 (41%), Positives = 60/109 (54%)
 Frame = -1

Query: 865 RLEICLCLFKPADIYLIDEPSQYLPHFNDFDDVADVIKSFFLHTKKAAVVAESSAYMAFL 686
           R+ I LCL KPADIYLIDEPS +L         + VIK F LH KK A + E    MA  
Sbjct: 473 RVAITLCLGKPADIYLIDEPSAHLDSEQRI-TASKVIKRFILHAKKTAFIVEHDFIMATY 531

Query: 685 LLDNRVILCDGKPSICCVATSPHEMFFNLVGILASDLEAIEADPSRNKP 539
           L D RVI+ +G+P++ C+A SP  +   +   L+        DP+  +P
Sbjct: 532 LAD-RVIVYEGQPAVKCIAHSPQSLLSGMNHFLSHLNITFRRDPTNFRP 579

 Score = 37.0 bits (84), Expect = 0.38
 Identities = 28/82 (34%), Positives = 34/82 (41%)
 Frame = -1

Query: 865 RLEICLCLFKPADIYLIDEPSQYLPHFNDFDDVADVIKSFFLHTKKAAVVAESSAYMAFL 686
           R  I     K ADIY+ DEPS YL         A VI+S   H     VV    + + + 
Sbjct: 224 RFAIAAVFVKKADIYMFDEPSSYL-DVRQRLKAAQVIRSLLRHDSYVIVVEHDLSVLDY- 281

Query: 685 LLDNRVILCDGKPSICCVATSP 620
            L + V    GKP    V T P
Sbjct: 282 -LSDFVCCLYGKPGAYGVVTLP 302

>ref|NP_193656.2| RNase L inhibitor protein, putative; protein id: At4g19210.1,
           supported by cDNA: gi_20466461 [Arabidopsis thaliana]
           gi|20466462|gb|AAM20548.1| RNase L inhibitor-like
           protein [Arabidopsis thaliana]
           gi|23198180|gb|AAN15617.1| RNase L inhibitor-like
           protein [Arabidopsis thaliana]
          Length = 605

 Score = 74.7 bits (182), Expect = 2e-12
 Identities = 47/109 (43%), Positives = 58/109 (53%)
 Frame = -1

Query: 865 RLEICLCLFKPADIYLIDEPSQYLPHFNDFDDVADVIKSFFLHTKKAAVVAESSAYMAFL 686
           R+ + LCL KPADIYLIDEPS YL         + VIK F LH KK A V E    MA  
Sbjct: 475 RVALTLCLGKPADIYLIDEPSAYLDSEQRI-VASKVIKRFILHAKKTAFVVEHDFIMATY 533

Query: 685 LLDNRVILCDGKPSICCVATSPHEMFFNLVGILASDLEAIEADPSRNKP 539
           L D RVI+ +G+PSI C A  P  +   +   L+        DP+  +P
Sbjct: 534 LAD-RVIVYEGQPSIDCTANCPQSLLSGMNLFLSHLNITFRRDPTNFRP 581

>pir||T04442 ABC-type transport protein T18B16.180 - Arabidopsis thaliana
           gi|2828296|emb|CAA16710.1| RNase L inhibitor-like
           protein [Arabidopsis thaliana]
           gi|7268716|emb|CAB78923.1| RNase L inhibitor-like
           protein [Arabidopsis thaliana]
          Length = 600

 Score = 74.7 bits (182), Expect = 2e-12
 Identities = 47/109 (43%), Positives = 58/109 (53%)
 Frame = -1

Query: 865 RLEICLCLFKPADIYLIDEPSQYLPHFNDFDDVADVIKSFFLHTKKAAVVAESSAYMAFL 686
           R+ + LCL KPADIYLIDEPS YL         + VIK F LH KK A V E    MA  
Sbjct: 470 RVALTLCLGKPADIYLIDEPSAYLDSEQRI-VASKVIKRFILHAKKTAFVVEHDFIMATY 528

Query: 685 LLDNRVILCDGKPSICCVATSPHEMFFNLVGILASDLEAIEADPSRNKP 539
           L D RVI+ +G+PSI C A  P  +   +   L+        DP+  +P
Sbjct: 529 LAD-RVIVYEGQPSIDCTANCPQSLLSGMNLFLSHLNITFRRDPTNFRP 576

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 728,111,319
Number of Sequences: 1393205
Number of extensions: 16326969
Number of successful extensions: 104171
Number of sequences better than 10.0: 89
Number of HSP's better than 10.0 without gapping: 60703
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 91577
length of database: 448,689,247
effective HSP length: 122
effective length of database: 278,718,237
effective search space used: 45988509105
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENLf063d06 BP065728 1 574 2 GNf071c12 BP072611 196 749 3 MPD015d04_f AV771019 234 728 4 MPD086f12_f AV775664 240 502 5 MPD085e11_f AV775589 242 478 6 MF041c05_f BP030429 242 820 7 GENf093e08 BP062224 246 398 8 MR045d05_f BP079477 246 393 9 GNf038e12 BP070160 247 786 10 GNf006h10 BP067843 252 845 11 MPD060h05_f AV774050 255 480 12 MR033b02_f BP078516 258 666 13 MF029h12_f BP029825 258 622 14 GNf047f08 BP070856 260 844 15 GENf026c12 BP059434 262 853 16 SPD029f03_f BP046312 263 897 17 SPDL078b08_f BP056830 266 842 18 MR059h12_f BP080562 267 838 19 SPD059b03_f BP048667 270 797 20 GENf021h01 BP059273 283 476 21 GENf016e11 BP059041 283 708 22 GNf039c09 BP070219 283 750 23 GNf099d03 BP074699 283 792 24 GNf033d12 BP069773 283 780 25 GNf056b02 BP071519 283 838 26 GNf100a12 BP074751 283 357 27 GENf038d09 BP059984 283 844 28 GENf032g10 BP059723 284 842 29 SPD003d09_f BP044235 284 816 30 GNf002a09 BP067489 284 891 31 GENf051d06 BP060521 285 851 32 GNf023d08 BP069037 295 756 33 GENf014e06 BP058938 296 862 34 MWM191f04_f AV767657 298 813 35 MWL001g06_f AV768603 298 718 36 GENf036b04 BP059871 299 703 37 SPD066e07_f BP049275 299 928 38 GNf002a03 BP067483 306 780 39 GNf024f12 BP069122 311 860 40 GENf010d05 BP058766 311 864 41 GENf069f04 BP061326 311 422 42 GENf078h12 BP061721 311 829 43 GNf002f05 BP067531 313 843 44 MPD042h12_f AV772896 329 439 45 GNf022c12 BP068960 381 843 46 MFB001a10_f BP033942 429 817

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