>KMC001043A_C01 KMC001043A_c01
agCTAAAATCGACATTCATTTAAAACTAAAATAATTGTAGCATTGTACTCTATTTCCCCC
CGCTTTTTTCCTGTTTTCCTTAAATTACAATTGTATACCTTGTAACCTACGCCCCCCCAA
AAATGACAACCAGGATAAATGACACCACTCACCAAACCCACAATAAAAAGATACAGCACC
CCATTCAATTTTAGGGCCAACACAGCAGAATATGGAGGGGCAAATTCTCCTAAAAAATTG
GAGAATGATGGATAAAAAAAATTGAATATAAGATATAATACACAACCAACTAGCCTAACC
AGCAGACTCAGGACCTTAACAAAATGGCCCTCCCAGAATAAGGCATTTTTACCAATCCTC
TATGATACACTCACCAAATTCAGCTCCAGTGGTCTCAAAGCTTCTTGAACTATTTCCTGA
ACTGCTCTGTTGCGCCGGCAGACATCCGCAAGCTCTTCAATGGGAACAGATAGTCCATTA
GTATCACCCCCAGAAGCCACATCAGAAAGTGCTTTTAAGAATCTTGATCGTTCCACGTCT
GTGGCAGCTGTTGATGGAATTAAAACAACACATTTCTTTGCCCATTCCAGGGCTTGCATC
CCATATGATCGAGTTAATGACAGTAATGTGTACGCTACCACATCAACTCGAGATTTCGGA
AGTGCTCCAGTTAATGAAGCAATCAAAATTCTGGTAATGCTGGGACCTCGAGGAATAATT
ACACTATCTCTGTAGGGTATAAATCGCTCCCCAAAAGTTGAGTTTCCAAGATC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001043A_C01 KMC001043A_c01
         (773 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_201065.1| unknown protein; protein id: At5g62590.1 [Arabi...   167  2e-40
gb|AAO00768.1| unknown protein [Arabidopsis thaliana]                 167  2e-40
ref|NP_010708.1| cell surface protein that may regulate cell wal...    35  0.92
sp|P41809|HKR1_YEAST HANSENULA MRAKII KILLER TOXIN-RESISTANT PRO...    35  0.92
ref|NP_717268.1| conserved hypothetical protein [Shewanella onei...    35  0.92

>ref|NP_201065.1| unknown protein; protein id: At5g62590.1 [Arabidopsis thaliana]
           gi|10178090|dbj|BAB11509.1| gene_id:K19B1.20~unknown
           protein [Arabidopsis thaliana]
          Length = 406

 Score =  167 bits (423), Expect = 2e-40
 Identities = 84/135 (62%), Positives = 106/135 (78%)
 Frame = -1

Query: 773 DLGNSTFGERFIPYRDSVIIPRGPSITRILIASLTGALPKSRVDVVAYTLLSLTRSYGMQ 594
           DL  S   E+F+  RD++IIPRG +ITRILIASL GALP SR+D V Y+LL+LTR+Y +Q
Sbjct: 268 DLEKSVNEEQFVRIRDNIIIPRGATITRILIASLAGALPSSRLDTVTYSLLALTRTYRLQ 327

Query: 593 ALEWAKKCVVLIPSTAATDVERSRFLKALSDVASGGDTNGLSVPIEELADVCRRNRAVQE 414
           A+ WAK+ V LIP TA T+ E ++FL+ALSD+A G D N L   +EEL+DVCRRNR VQE
Sbjct: 328 AVSWAKESVSLIPRTALTETESTKFLQALSDIAYGADVNSLIGQVEELSDVCRRNRTVQE 387

Query: 413 IVQEALRPLELNLVS 369
           +VQ AL+PLELNLV+
Sbjct: 388 LVQAALKPLELNLVT 402

>gb|AAO00768.1| unknown protein [Arabidopsis thaliana]
          Length = 958

 Score =  167 bits (423), Expect = 2e-40
 Identities = 84/135 (62%), Positives = 106/135 (78%)
 Frame = -1

Query: 773  DLGNSTFGERFIPYRDSVIIPRGPSITRILIASLTGALPKSRVDVVAYTLLSLTRSYGMQ 594
            DL  S   E+F+  RD++IIPRG +ITRILIASL GALP SR+D V Y+LL+LTR+Y +Q
Sbjct: 820  DLEKSVNEEQFVRIRDNIIIPRGATITRILIASLAGALPSSRLDTVTYSLLALTRTYRLQ 879

Query: 593  ALEWAKKCVVLIPSTAATDVERSRFLKALSDVASGGDTNGLSVPIEELADVCRRNRAVQE 414
            A+ WAK+ V LIP TA T+ E ++FL+ALSD+A G D N L   +EEL+DVCRRNR VQE
Sbjct: 880  AVSWAKESVSLIPRTALTETESTKFLQALSDIAYGADVNSLIGQVEELSDVCRRNRTVQE 939

Query: 413  IVQEALRPLELNLVS 369
            +VQ AL+PLELNLV+
Sbjct: 940  LVQAALKPLELNLVT 954

>ref|NP_010708.1| cell surface protein that may regulate cell wall beta-glucan
           synthesis and bud site selection; Hanenula mrakii killer
           toxin-resistance protein; Hkr1p [Saccharomyces
           cerevisiae] gi|2131284|pir||S69703 HKR1 protein
           precursor - yeast (Saccharomyces cerevisiae)
           gi|927691|gb|AAB64857.1| Hkr1p; YDR420W; CAI: 0.10
           [Saccharomyces cerevisiae]
          Length = 1802

 Score = 35.4 bits (80), Expect = 0.92
 Identities = 22/76 (28%), Positives = 35/76 (45%), Gaps = 10/76 (13%)
 Frame = +1

Query: 469 DSPLVSPPEATSESAFKNLDRSTSVAAVDGIKTTHFFAHSRA----------CIPYDRVN 618
           D P  S    TS  A + + R ++  AV  I TT+F   S +           +PY  V+
Sbjct: 349 DRPATSSTAETSSEASQGVSRESNTFAVSSISTTNFIVSSASDTVVSTSSTNTVPYSSVH 408

Query: 619 DSNVYATTSTRDFGSA 666
            + V+AT+S+    S+
Sbjct: 409 STFVHATSSSTYISSS 424

>sp|P41809|HKR1_YEAST HANSENULA MRAKII KILLER TOXIN-RESISTANT PROTEIN 1 PRECURSOR
           gi|545660|gb|AAB30051.1| Hkr1p [Saccharomyces
           cerevisiae]
          Length = 1802

 Score = 35.4 bits (80), Expect = 0.92
 Identities = 22/76 (28%), Positives = 35/76 (45%), Gaps = 10/76 (13%)
 Frame = +1

Query: 469 DSPLVSPPEATSESAFKNLDRSTSVAAVDGIKTTHFFAHSRA----------CIPYDRVN 618
           D P  S    TS  A + + R ++  AV  I TT+F   S +           +PY  V+
Sbjct: 349 DRPATSSTAETSSEASQGVSRESNTFAVSSISTTNFIVSSASDTVVSTSSTNTVPYSSVH 408

Query: 619 DSNVYATTSTRDFGSA 666
            + V+AT+S+    S+
Sbjct: 409 STFVHATSSSTYISSS 424

>ref|NP_717268.1| conserved hypothetical protein [Shewanella oneidensis MR-1]
           gi|24347454|gb|AAN54712.1|AE015612_1 conserved
           hypothetical protein [Shewanella oneidensis MR-1]
          Length = 244

 Score = 35.4 bits (80), Expect = 0.92
 Identities = 28/86 (32%), Positives = 44/86 (50%), Gaps = 1/86 (1%)
 Frame = -1

Query: 725 SVIIPRGPSITRILIA-SLTGALPKSRVDVVAYTLLSLTRSYGMQALEWAKKCVVLIPST 549
           S+II R P + +  IA + T  L  S    V Y +  L   Y + A     +CVV++  +
Sbjct: 25  SLIIWRRPRLAKWAIAGAFTLLLVLSSQFSVDYLVKPLETQYPINASSITGQCVVMVLGS 84

Query: 548 AATDVERSRFLKALSDVASGGDTNGL 471
           A +D+E +  +++LS VA    T GL
Sbjct: 85  AHSDIEDATAVQSLSAVALARLTEGL 110

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 693,042,498
Number of Sequences: 1393205
Number of extensions: 15847919
Number of successful extensions: 40174
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 38179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 40005
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 38095156112
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD024g06_f AV771668 1 482 2 MFBL004a06_f BP041452 3 516 3 GENLf061c11 BP065605 3 530 4 MRL024c12_f BP084951 7 471 5 MPDL027f07_f AV777862 16 540 6 GENLf053d04 BP065176 18 513 7 MRL008f01_f BP084109 47 464 8 MPDL047a03_f AV778854 213 778

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