>KMC001016A_C02 KMC001016A_c02
CAAAACCAACTACAAAGACATGGACTAATTTCACCATTAACGCTAAGTAAACAACACAAG
TCATTTATCGCATTGCTGCTTTACTTTGGATTAGAGCACTTGATTTGTAACCAAATATTG
AGGAACAACAAACATTAACCAATGGGGACATACAAAGACCCACTATTTCTAACATCTCAC
CTTGTGGTTACATAAGCCAGTGTAGAATGATCCACAACAACCAATATTTGTAAAAAAAAA
CTATTCTCTTGGAGTGATGCAGAGTAGAAGAGCTGTGGTTTGTATTGCTGATACGCATAG
GGCATTCAATGCCGCAGTAAAGAGATGCCATATGTGATTAATCATGAAGAACCATGGCCT
AGGTCCTTGGATATGATGGGCATTTAGCTTGAACAGGTAATGATGAAGTAGCAACTCTGG
CCAAGCCTATTTTGAAGCTAGAACACTAGAGTTCAGGAATTCAAGGCGTTTTTTGGTTAA
AGTGATCTTTTCTTCTGCTTCCGCTGCTTTTTCCTGCACCCCACGGACCACATTTTCTGG
GGCCTTCTCCACAAATTGAGGAGAACTGATTTTAGCTATCAATGCGTCATACTCCTTCTG
CATCTTGGAAAGGCGTTTTGAGAGACGTTGAGCTTCTGCAGAAATATC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC001016A_C02 KMC001016A_c02
         (648 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T51503 valine-tRNA ligase-like protein - Arabidopsis thalia...    99  6e-20
dbj|BAC20772.1| valine--tRNA ligase-like protein [Oryza sativa (...    99  6e-20
ref|NP_568337.1| valine--tRNA ligase-like protein; protein id: A...    99  6e-20
ref|NP_764894.1| valine-tRNA ligase [Staphylococcus epidermidis ...    52  9e-06
ref|NP_374775.1| valine-tRNA ligase [Staphylococcus aureus subsp...    51  2e-05

>pir||T51503 valine-tRNA ligase-like protein - Arabidopsis thaliana
            gi|9755723|emb|CAC01835.1| valine--tRNA ligase-like
            protein [Arabidopsis thaliana]
          Length = 1093

 Score = 98.6 bits (244), Expect = 6e-20
 Identities = 49/68 (72%), Positives = 58/68 (85%)
 Frame = -1

Query: 648  DISAEAQRLSKRLSKMQKEYDALIAKISSPQFVEKAPENVVRGVQEKAAEAEEKITLTKK 469
            DIS+E QR+SKRLSKMQ EYDALI ++SSP+FVEKAPE VVRGV+E+  E EEKI LTK 
Sbjct: 1020 DISSEVQRISKRLSKMQTEYDALITRLSSPKFVEKAPEEVVRGVKEQVEELEEKIKLTKA 1079

Query: 468  RLEFLNSS 445
            RL+FL S+
Sbjct: 1080 RLDFLKST 1087

>dbj|BAC20772.1| valine--tRNA ligase-like protein [Oryza sativa (japonica
            cultivar-group)]
          Length = 985

 Score = 98.6 bits (244), Expect = 6e-20
 Identities = 48/69 (69%), Positives = 60/69 (86%)
 Frame = -1

Query: 648  DISAEAQRLSKRLSKMQKEYDALIAKISSPQFVEKAPENVVRGVQEKAAEAEEKITLTKK 469
            D+S E +RLSKRLSKMQ EYD+L+A+++S  FVEKAPE +VRGV+EKA+EAEEKI+LTK 
Sbjct: 915  DVSEEVKRLSKRLSKMQSEYDSLLARLNSGSFVEKAPEEIVRGVREKASEAEEKISLTKN 974

Query: 468  RLEFLNSSV 442
            RL FL S+V
Sbjct: 975  RLAFLQSTV 983

>ref|NP_568337.1| valine--tRNA ligase-like protein; protein id: At5g16715.1
            [Arabidopsis thaliana]
          Length = 884

 Score = 98.6 bits (244), Expect = 6e-20
 Identities = 49/68 (72%), Positives = 58/68 (85%)
 Frame = -1

Query: 648  DISAEAQRLSKRLSKMQKEYDALIAKISSPQFVEKAPENVVRGVQEKAAEAEEKITLTKK 469
            DIS+E QR+SKRLSKMQ EYDALI ++SSP+FVEKAPE VVRGV+E+  E EEKI LTK 
Sbjct: 811  DISSEVQRISKRLSKMQTEYDALITRLSSPKFVEKAPEEVVRGVKEQVEELEEKIKLTKA 870

Query: 468  RLEFLNSS 445
            RL+FL S+
Sbjct: 871  RLDFLKST 878

>ref|NP_764894.1| valine-tRNA ligase [Staphylococcus epidermidis ATCC 12228]
            gi|27315803|gb|AAO04938.1|AE016748_172 valine-tRNA ligase
            [Staphylococcus epidermidis ATCC 12228]
          Length = 876

 Score = 51.6 bits (122), Expect = 9e-06
 Identities = 26/67 (38%), Positives = 37/67 (54%)
 Frame = -1

Query: 648  DISAEAQRLSKRLSKMQKEYDALIAKISSPQFVEKAPENVVRGVQEKAAEAEEKITLTKK 469
            D+  E  RL K L K+Q E D +  K+S+  FV KAPE ++   +EK    +EK    K 
Sbjct: 810  DMDKEIARLEKELDKLQSELDRVDKKLSNENFVNKAPEKIINEEKEKQQHYQEKYNGVKS 869

Query: 468  RLEFLNS 448
            R+E L +
Sbjct: 870  RIEQLKA 876

>ref|NP_374775.1| valine-tRNA ligase [Staphylococcus aureus subsp. aureus N315]
            gi|21283336|ref|NP_646424.1| valine-tRNA ligase
            [Staphylococcus aureus subsp. aureus MW2]
            gi|25293106|pir||E89949 valine-tRNA ligase [imported] -
            Staphylococcus aureus  (strain N315)
            gi|13701460|dbj|BAB42754.1| valine-tRNA ligase
            [Staphylococcus aureus subsp. aureus N315]
            gi|21204776|dbj|BAB95472.1| valine-tRNA ligase
            [Staphylococcus aureus subsp. aureus MW2]
          Length = 876

 Score = 50.8 bits (120), Expect = 2e-05
 Identities = 26/67 (38%), Positives = 38/67 (55%)
 Frame = -1

Query: 648  DISAEAQRLSKRLSKMQKEYDALIAKISSPQFVEKAPENVVRGVQEKAAEAEEKITLTKK 469
            D+  E  RL K L+K+Q E D +  K+S+  FV KAPE V+   + K  + +EK    K 
Sbjct: 810  DMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRKKQDYQEKYDGVKA 869

Query: 468  RLEFLNS 448
            R+E L +
Sbjct: 870  RIEQLKA 876

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 550,322,284
Number of Sequences: 1393205
Number of extensions: 11706117
Number of successful extensions: 26437
Number of sequences better than 10.0: 88
Number of HSP's better than 10.0 without gapping: 25563
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26424
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 27576232529
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNLf010d03 BP075392 1 492 2 MPDL028b05_f AV777889 50 612 3 GNf048d09 BP070915 67 563 4 GENLf051e07 BP065076 87 181 5 MPDL053a06_f AV779166 88 590 6 GENLf079a12 BP066621 119 649

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