Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000984A_C01 KMC000984A_c01
(531 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T30560 resistance protein RGC20 - garden lettuce (fragment)... 43 0.002
pir||T30562 resistance protein RGC2B - garden lettuce gi|4106970... 38 0.065
ref|NP_201490.1| disease resistance protein (NBS-LRR class), put... 37 0.11
gb|AAO45749.1| MRGH63 [Cucumis melo] 37 0.11
pir||T30559 resistance protein RGC2K - garden lettuce gi|4139038... 37 0.11
>pir||T30560 resistance protein RGC20 - garden lettuce (fragment)
gi|4139041|gb|AAD03673.1| resistance protein candidate
RGC20 [Lactuca sativa]
Length = 1758
Score = 43.1 bits (100), Expect = 0.002
Identities = 33/131 (25%), Positives = 59/131 (44%), Gaps = 20/131 (15%)
Frame = -3
Query: 454 SWKIDIQSCKKLNSLFCSADSCCKSSSHLYSTLESIKVEYCDSMKDIF------------ 311
S +I+I+SC L+S+ C +S + L +K+E C +K++F
Sbjct: 1290 SREIEIRSCHALSSVIP-----CYASGQMQK-LRVLKIERCKGVKEVFETQGICSNKNNK 1343
Query: 310 -----ANAPQPFPGAFASLKHIYIYD---CSALEYLLTAEIVPQLQNLETIAVIDCKSMK 155
N P + L ++ I + C +LE++ T + L+ LE + ++DC SMK
Sbjct: 1344 SGCDEGNDEIPRVNSIIMLPNLMILEISKCGSLEHIFTFSALESLRQLEELMILDCGSMK 1403
Query: 154 EVFANYFISSN 122
+ SS+
Sbjct: 1404 VIVKEEHASSS 1414
Score = 36.6 bits (83), Expect = 0.19
Identities = 19/79 (24%), Positives = 40/79 (50%), Gaps = 5/79 (6%)
Frame = -3
Query: 370 LYSTLESIKVEYCDSMKDIFA-----NAPQPFPGAFASLKHIYIYDCSALEYLLTAEIVP 206
++ LE + + Y D+M ++ Q F +L I++ DC +++YL + +
Sbjct: 1147 IFPNLEELYLYYMDNMSHVWKCNNWNKFLQQSESPFHNLTTIHMSDCKSIKYLFSPLMAE 1206
Query: 205 QLQNLETIAVIDCKSMKEV 149
L NL+ I + +C ++E+
Sbjct: 1207 LLSNLKRINIDECDGIEEI 1225
Score = 35.4 bits (80), Expect = 0.42
Identities = 32/131 (24%), Positives = 53/131 (40%), Gaps = 18/131 (13%)
Frame = -3
Query: 448 KIDIQSCKKLNSLFCSAD-------SCCKSSSHLYSTLESIKVEYCDSMKDI-------- 314
+I+I C + + D + SS+ L+ L+S+ + D++K I
Sbjct: 1213 RINIDECDGIEEIVSKRDDVDEEMTTSTHSSTILFPHLDSLTLFRLDNLKCIGGGGAFLD 1272
Query: 313 ---FANAPQPFPGAFASLKHIYIYDCSALEYLLTAEIVPQLQNLETIAVIDCKSMKEVFA 143
F+ A + I I C AL ++ Q+Q L + + CK +KEVF
Sbjct: 1273 RFKFSQAGVVCWSLCQYSREIEIRSCHALSSVIPCYASGQMQKLRVLKIERCKGVKEVFE 1332
Query: 142 NYFISSNEGVK 110
I SN+ K
Sbjct: 1333 TQGICSNKNNK 1343
>pir||T30562 resistance protein RGC2B - garden lettuce gi|4106970|gb|AAD03156.1|
resistance protein candidate RGC2B [Lactuca sativa]
Length = 1810
Score = 38.1 bits (87), Expect = 0.065
Identities = 19/65 (29%), Positives = 31/65 (47%)
Frame = -3
Query: 334 CDSMKDIFANAPQPFPGAFASLKHIYIYDCSALEYLLTAEIVPQLQNLETIAVIDCKSMK 155
C + F Q F +L I I C +++YL + + L NL+ I + +C +K
Sbjct: 1151 CSNWNKFFTLPKQQSESPFHNLTTINILKCKSIKYLFSPLMAELLSNLKDIRISECDGIK 1210
Query: 154 EVFAN 140
EV +N
Sbjct: 1211 EVVSN 1215
Score = 33.5 bits (75), Expect = 1.6
Identities = 27/120 (22%), Positives = 52/120 (42%), Gaps = 20/120 (16%)
Frame = -3
Query: 448 KIDIQSCKKLNSLFCSADSCCKSSSHLYSTLESIKVEYCDSMKDIF-------ANAPQPF 290
+I+I C L+S+ C ++ + L+ +++E CD MK++F +N
Sbjct: 1299 EIEIVGCYALSSVIP-----CYAAGQMQK-LQVLRIESCDGMKEVFETQLGTSSNKNNEK 1352
Query: 289 PGA-------------FASLKHIYIYDCSALEYLLTAEIVPQLQNLETIAVIDCKSMKEV 149
G +LK + I +C LE++ T + L+ L+ + + C MK +
Sbjct: 1353 SGCEEGIPRVNNNVIMLPNLKILSIGNCGGLEHIFTFSALESLRQLQELKIKFCYGMKVI 1412
>ref|NP_201490.1| disease resistance protein (NBS-LRR class), putative; protein id:
At5g66890.1 [Arabidopsis thaliana]
gi|9758139|dbj|BAB08631.1| contains similarity to
disease resistance protein~gene_id:MUD21.15 [Arabidopsis
thaliana]
Length = 415
Score = 37.4 bits (85), Expect = 0.11
Identities = 26/103 (25%), Positives = 53/103 (51%), Gaps = 3/103 (2%)
Frame = -3
Query: 448 KIDIQSCKKLNSLFCSADSCCKSSSHLYSTLES---IKVEYCDSMKDIFANAPQPFPGAF 278
K+ ++S +KL+ FC + TL+S I+++YC ++ ++ Q
Sbjct: 224 KLGLKSLEKLSLWFCHVVDALNELEDVSETLQSLQEIEIDYCYNLDELPYWISQ-----V 278
Query: 277 ASLKHIYIYDCSALEYLLTAEIVPQLQNLETIAVIDCKSMKEV 149
SLK + + +C+ L ++ E + L++LET+ + C S+ E+
Sbjct: 279 VSLKKLSVTNCNKLCRVI--EAIGDLRDLETLRLSSCASLLEL 319
>gb|AAO45749.1| MRGH63 [Cucumis melo]
Length = 943
Score = 37.4 bits (85), Expect = 0.11
Identities = 29/113 (25%), Positives = 47/113 (40%)
Frame = -3
Query: 508 NQWTCLCNGLSSEGTSSSSWKIDIQSCKKLNSLFCSADSCCKSSSHLYSTLESIKVEYCD 329
N+ L S +G + ++S K L+ C D C S ++E + + Y
Sbjct: 674 NKLVALHLSSSVKGFEQFPSHLKLKSLKFLSMKNCRIDEWCPQFSEEMKSIEYLSIGY-- 731
Query: 328 SMKDIFANAPQPFPGAFASLKHIYIYDCSALEYLLTAEIVPQLQNLETIAVID 170
I + P G SLKH+ +Y C L L + +L NL ++ V+D
Sbjct: 732 ---SIVTHQLSPTIGYLTSLKHLTLYYCKELTTL--PSTIYRLSNLTSLIVLD 779
Score = 32.3 bits (72), Expect = 3.6
Identities = 25/96 (26%), Positives = 42/96 (43%), Gaps = 3/96 (3%)
Frame = -3
Query: 280 FASLKHIYIYDCSALEYLLTAEIVPQLQNLETIAVIDCKSM---KEVFANYFISSNEGVK 110
F SLK++Y DC LE EI + + + CKS+ + A++ N V+
Sbjct: 846 FKSLKYLYTMDCELLE-----EISKVPEGVICTSAAGCKSLARFPDNLADFISCGNSAVR 900
Query: 109 HLSE*HHIHILLPKL*DMRCVCPFTYILFSPYIFHL 2
+S H I+ C+ F +I++ Y + L
Sbjct: 901 TISLSHDFTII-----SSSCIFNFIHIIYITYNYLL 931
>pir||T30559 resistance protein RGC2K - garden lettuce gi|4139038|gb|AAD03672.1|
resistance protein candidate RGC2K [Lactuca sativa]
Length = 1715
Score = 37.4 bits (85), Expect = 0.11
Identities = 19/68 (27%), Positives = 35/68 (50%)
Frame = -3
Query: 268 KHIYIYDCSALEYLLTAEIVPQLQNLETIAVIDCKSMKEVFANYFISSNEGVKHLSE*HH 89
+ I I +C AL ++ Q+QN++ + + C SMKE+F +++N G E +
Sbjct: 1090 REINIRECYALSSVIPCYAAGQMQNVQVLNIYRCNSMKELFETQGMNNNNGDSGCDEGNG 1149
Query: 88 IHILLPKL 65
+P+L
Sbjct: 1150 CIPAIPRL 1157
Score = 33.5 bits (75), Expect = 1.6
Identities = 15/42 (35%), Positives = 25/42 (58%)
Frame = -3
Query: 274 SLKHIYIYDCSALEYLLTAEIVPQLQNLETIAVIDCKSMKEV 149
+LK + I DC LE++ T + L+ LE + + CK+MK +
Sbjct: 1165 NLKILKIEDCGHLEHVFTFSALGSLRQLEELTIEKCKAMKVI 1206
Score = 31.6 bits (70), Expect = 6.1
Identities = 23/80 (28%), Positives = 36/80 (44%), Gaps = 1/80 (1%)
Frame = -3
Query: 448 KIDIQSCKKLNSLF-CSADSCCKSSSHLYSTLESIKVEYCDSMKDIFANAPQPFPGAFAS 272
K+DI K L ++ C S STL IKV CD++ ++F P P
Sbjct: 900 KLDISYMKDLKEIWPCELGM---SQEVDVSTLRVIKVSSCDNLVNLFPCNPMPL---IHH 953
Query: 271 LKHIYIYDCSALEYLLTAEI 212
L+ + + C ++E L E+
Sbjct: 954 LEELQVIFCGSIEVLFNIEL 973
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 417,228,275
Number of Sequences: 1393205
Number of extensions: 8049253
Number of successful extensions: 22692
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 21855
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22646
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17596710992
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
