>KMC000976A_C02 KMC000976A_c02
GGCGTAAGTACACTTAGCTTCCATTCATGTACTGTGAAAAATTATAAAGTCCTGCATCCA
ATTTGAGCGCATGCCAAAAAACTACAAGTTGGATCAGATCTCAGAAGAATAAAGTCACAA
GGATAGCCAACATTTTACAAGAGCTCCACCACTTTGAATCTTCCATTATAAGCCTCCCAT
TATTGGAAATAACATCGTTACTTGAGTTCTAGTCCTCAGAAAAAGAATCAATATCTTCTG
TTCTTCTAGCATGAAAATATTTGCCTGGTGTATTTGAATTCAGTTTCATTTTTCAGATAG
AGTAGCTATCAGCTCGGATTTTGCCTTCTCGAGAGCACTGGTCAAGTTTTCAGGCTTCCT
CCCACCAGCCTGAGCAAAATTAGGCCTTCCACCTCCGCCGCCACCACATAACTTTGCTAT
TTGCCCAATAAACTTACCTGCCTGAATCCCTCGATCAACCACCCCTGGAGTAAAAGCAGC
CACTAGACTAACCTTCCCTTCACCTGGACATGAGCCCAGTACAACAGCTG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000976A_C02 KMC000976A_c02
         (530 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_680210.1| similar to alanine--tRNA ligase, putative; prot...   132  3e-30
ref|NP_486458.1| alanyl-tRNA synthetase [Nostoc sp. PCC 7120] gi...   103  1e-21
ref|ZP_00072711.1| hypothetical protein [Trichodesmium erythraeu...   101  5e-21
gb|ZP_00107033.1| hypothetical protein [Nostoc punctiforme]            99  3e-20
ref|NP_441845.1| alanyl-tRNA synthetase [Synechocystis sp. PCC 6...    94  8e-19

>ref|NP_680210.1| similar to alanine--tRNA ligase, putative; protein id: At5g22800.1
            [Arabidopsis thaliana] gi|10177227|dbj|BAB10601.1|
            alanyl-tRNA synthetase [Arabidopsis thaliana]
          Length = 954

 Score =  132 bits (332), Expect = 3e-30
 Identities = 65/80 (81%), Positives = 72/80 (89%)
 Frame = -3

Query: 528  AVVLGSCPGEGKVSLVAAFTPGVVDRGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKPE 349
            AVVLGS P + KVSLVAAF+PGVV  G+QAGKFIG IAKLCGGGGGG+PNFAQAGGRKPE
Sbjct: 873  AVVLGSSPEKDKVSLVAAFSPGVVSLGVQAGKFIGPIAKLCGGGGGGKPNFAQAGGRKPE 932

Query: 348  NLTSALEKAKSELIATLSEK 289
            NL SALEKA+ +L+ATLSEK
Sbjct: 933  NLPSALEKAREDLVATLSEK 952

>ref|NP_486458.1| alanyl-tRNA synthetase [Nostoc sp. PCC 7120]
            gi|20532251|sp|Q8YUD4|SYA_ANASP Alanyl-tRNA synthetase
            (Alanine--tRNA ligase) (AlaRS) gi|25293093|pir||AC2108
            alanyl-tRNA synthetase [imported] - Nostoc sp. (strain
            PCC 7120) gi|17131510|dbj|BAB74117.1| alanyl-tRNA
            synthetase [Nostoc sp. PCC 7120]
          Length = 880

 Score =  103 bits (258), Expect = 1e-21
 Identities = 53/77 (68%), Positives = 61/77 (78%)
 Frame = -3

Query: 528  AVVLGSCPGEGKVSLVAAFTPGVVDRGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKPE 349
            AVVLGS P   KVSLVAAF+P V  +G+QAGKFIG IAK+CGGGGGGRPN AQAGGR   
Sbjct: 803  AVVLGSVPEADKVSLVAAFSPEVNKKGLQAGKFIGAIAKICGGGGGGRPNLAQAGGRDAS 862

Query: 348  NLTSALEKAKSELIATL 298
             L +ALE+A+SEL + L
Sbjct: 863  KLPAALEQAQSELKSAL 879

>ref|ZP_00072711.1| hypothetical protein [Trichodesmium erythraeum IMS101]
          Length = 897

 Score =  101 bits (252), Expect = 5e-21
 Identities = 52/74 (70%), Positives = 58/74 (78%)
 Frame = -3

Query: 528  AVVLGSCPGEGKVSLVAAFTPGVVDRGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKPE 349
            AVVLGS   EGKVSLVAAF+  V  +G+QAGKFIG IAK+CGGGGGGRPN AQAGGR P 
Sbjct: 821  AVVLGSA-AEGKVSLVAAFSKSVNGKGLQAGKFIGGIAKICGGGGGGRPNLAQAGGRDPS 879

Query: 348  NLTSALEKAKSELI 307
             L  ALE AK +L+
Sbjct: 880  KLKEALESAKEQLV 893

>gb|ZP_00107033.1| hypothetical protein [Nostoc punctiforme]
          Length = 880

 Score = 99.0 bits (245), Expect = 3e-20
 Identities = 49/78 (62%), Positives = 60/78 (76%)
 Frame = -3

Query: 528  AVVLGSCPGEGKVSLVAAFTPGVVDRGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKPE 349
            AVVLGS P   KVS+VAAF+P V  +G+QAGKF+G IAK+CGGGGGGRPN AQAGGR   
Sbjct: 803  AVVLGSVPEADKVSIVAAFSPEVNKKGLQAGKFVGAIAKICGGGGGGRPNLAQAGGRDAS 862

Query: 348  NLTSALEKAKSELIATLS 295
             L  AL +A+S+L + L+
Sbjct: 863  KLPDALGQAESDLKSALA 880

>ref|NP_441845.1| alanyl-tRNA synthetase [Synechocystis sp. PCC 6803]
            gi|2500959|sp|P74423|SYA_SYNY3 Alanyl-tRNA synthetase
            (Alanine--tRNA ligase) (AlaRS) gi|7437614|pir||S76394
            hypothetical protein - Synechocystis sp. (strain PCC
            6803) gi|1653611|dbj|BAA18523.1| alanyl-tRNA synthetase
            [Synechocystis sp. PCC 6803]
          Length = 877

 Score = 94.4 bits (233), Expect = 8e-19
 Identities = 50/78 (64%), Positives = 58/78 (74%), Gaps = 1/78 (1%)
 Frame = -3

Query: 528  AVVLGSCPGEGKVSLVAAFTPGVVD-RGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKP 352
            AVVL S P EGKVSLVAAF+P +V  + ++AG+FIG IAK+CGGGGGGRPN AQAGGR  
Sbjct: 799  AVVLASIPEEGKVSLVAAFSPQLVKTKQLKAGQFIGAIAKICGGGGGGRPNLAQAGGRDA 858

Query: 351  ENLTSALEKAKSELIATL 298
              L  AL  AK  L+A L
Sbjct: 859  SKLPEALATAKQTLLAEL 876

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 474,892,806
Number of Sequences: 1393205
Number of extensions: 10819044
Number of successful extensions: 52242
Number of sequences better than 10.0: 217
Number of HSP's better than 10.0 without gapping: 33744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 44643
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17596710992
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MWL063h04_f AV769695 1 371 2 MPDL074c01_f AV780295 2 524 3 MFBL033f10_f BP042929 2 516 4 GENLf048d07 BP064903 19 527 5 SPDL058b09_f BP055615 37 223 6 GENLf068f06 BP066026 37 531

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