>KMC000927A_C01 KMC000927A_c01
gaGAAAAAGAAAAAAAATGACATTCATTTAAAAAAAGAAAGAAACATGCTTGGAAGTTGG
ATCATAGATACAATAATCCAATAACTCAGTGGCTTCCCTCTCAGTGAAGAGTAAAACTAG
AAAGCAAGTTATCAGTCATGCAAAGCAAATAAGGGATAAAAAAAGACCCTTAACATTGTT
CACAATGAAAAAAAAAACTGAATAATAATAATAATTATATATCTGAGTCAATCCCATGCT
CAGAATATATACTGAACATATGACAACTTGGAAGAGCTAGGCCAAGGTACTTGAATAATG
ACCATGGAACCTTCATATTCAAAATCAGCTTCTTTCCCATCAATTCTACAAGCACTTGGT
CTCTCTGAAGAAAACACTCTCATCTCTCCTGTGCCTCTTACTCCAACTGGAACCAAATTC
TGAGCCTCATCAAAATCCAAGGATGGAATGGCTCCACCAGTGTTGAGCATATTCACCAAA
CCAACAGGAGCAAACTGCACAGACTTGGAAGCCAAGGAGGCAGTGACAACAGGGGAAACC
GTTACGAGCTCGAAATCG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000927A_C01 KMC000927A_c01
         (558 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAD20127.2| raffinose synthase [Pisum sativum]                    123  1e-27
gb|AAD02832.1| raffinose synthase [Cucumis sativus]                   120  1e-26
ref|NP_198855.1| glycosyl hydrolase family 36; protein id: At5g4...   113  2e-24
dbj|BAB64768.1| putative raffinose synthase [Oryza sativa (japon...    89  4e-17
gb|AAL90901.1| AT5g20250/F5O24_140 [Arabidopsis thaliana] gi|233...    66  3e-10

>emb|CAD20127.2| raffinose synthase [Pisum sativum]
          Length = 798

 Score =  123 bits (309), Expect = 1e-27
 Identities = 57/104 (54%), Positives = 80/104 (76%)
 Frame = -2

Query: 554  FELVTVSPVVTASLASKSVQFAPVGLVNMLNTGGAIPSLDFDEAQNLVPVGVRGTGEMRV 375
            FEL+TVSP+   S   + +QFAP+GLVNMLN+GGA+ SL+FD++ +LV +GVRG GE+ V
Sbjct: 697  FELMTVSPLKVFS--KRLIQFAPIGLVNMLNSGGAVQSLEFDDSASLVKIGVRGCGELSV 754

Query: 374  FSSERPSACRIDGKEADFEYEGSMVIIQVPWPSSSKLSYVQYIF 243
            F+SE+P  C+IDG   +F+YE  MV +Q+ WP SS LS V+++F
Sbjct: 755  FASEKPVCCKIDGVSVEFDYEDKMVRVQILWPGSSTLSLVEFLF 798

>gb|AAD02832.1| raffinose synthase [Cucumis sativus]
          Length = 784

 Score =  120 bits (301), Expect = 1e-26
 Identities = 59/109 (54%), Positives = 82/109 (75%), Gaps = 4/109 (3%)
 Frame = -2

Query: 557 DFELVTVSPVVTASLASKSVQFAPVGLVNMLNTGGAIPSLDFDEAQNLVPVGVRGTGEMR 378
           +FEL+TVSPV    L   S+ FAP+GLVNMLNT GAI S+D+D+  + V +GV+G GEMR
Sbjct: 678 EFELITVSPVT--KLIQTSLHFAPIGLVNMLNTSGAIQSVDYDDDLSSVEIGVKGCGEMR 735

Query: 377 VFSSERPSACRIDGKEADFEY-EGSMVIIQVPWP---SSSKLSYVQYIF 243
           VF+S++P ACRIDG++  F+Y +  MV++QVPWP   SS  +S ++Y+F
Sbjct: 736 VFASKKPRACRIDGEDVGFKYDQDQMVVVQVPWPIDSSSGGISVIEYLF 784

>ref|NP_198855.1| glycosyl hydrolase family 36; protein id: At5g40390.1, supported by
           cDNA: gi_17065409, supported by cDNA: gi_20148632
           [Arabidopsis thaliana] gi|10178150|dbj|BAB11595.1|
           raffinose synthase protein [Arabidopsis thaliana]
           gi|17065410|gb|AAL32859.1| raffinose synthase protein
           [Arabidopsis thaliana] gi|20148633|gb|AAM10207.1|
           raffinose synthase protein [Arabidopsis thaliana]
          Length = 783

 Score =  113 bits (282), Expect = 2e-24
 Identities = 58/104 (55%), Positives = 76/104 (72%)
 Frame = -2

Query: 554 FELVTVSPVVTASLASKSVQFAPVGLVNMLNTGGAIPSLDFDEAQNLVPVGVRGTGEMRV 375
           FEL+TVSPVVT  +   SV+FAP+GLVNMLNT GAI SL +++    V VGV G GE RV
Sbjct: 684 FELITVSPVVT--IEGNSVRFAPIGLVNMLNTSGAIRSLVYNDES--VEVGVFGAGEFRV 739

Query: 374 FSSERPSACRIDGKEADFEYEGSMVIIQVPWPSSSKLSYVQYIF 243
           ++S++P +C IDG+  +F YE SMV++QVPW     LS +QY+F
Sbjct: 740 YASKKPVSCLIDGEVVEFGYEDSMVMVQVPWSGPDGLSSIQYLF 783

>dbj|BAB64768.1| putative raffinose synthase [Oryza sativa (japonica cultivar-group)]
            gi|20804839|dbj|BAB92522.1| putative raffinose synthase
            [Oryza sativa (japonica cultivar-group)]
          Length = 816

 Score = 89.0 bits (219), Expect = 4e-17
 Identities = 45/107 (42%), Positives = 65/107 (60%), Gaps = 3/107 (2%)
 Frame = -2

Query: 554  FELVTVSPVVTASLASKSVQFAPVGLVNMLNTGGAIPSLDFDEAQNLVP--VGVRGTGEM 381
            +EL+ V+PV         + FAP+GL NMLN GGA+   +       V   V V+G GEM
Sbjct: 710  YELLVVAPVRAIVSPELGIGFAPIGLANMLNAGGAVQGFEAARKDGDVAAEVAVKGAGEM 769

Query: 380  RVFSSERPSACRIDGKEADFEYEGSMVIIQVPWP-SSSKLSYVQYIF 243
              +SS RP  C+++G++A+F+YE  +V + VPW  SS KLS V+Y +
Sbjct: 770  VAYSSARPRLCKVNGQDAEFKYEDGIVTVDVPWTGSSKKLSRVEYFY 816

>gb|AAL90901.1| AT5g20250/F5O24_140 [Arabidopsis thaliana]
           gi|23308457|gb|AAN18198.1| At5g20250/F5O24_140
           [Arabidopsis thaliana]
          Length = 844

 Score = 65.9 bits (159), Expect = 3e-10
 Identities = 34/88 (38%), Positives = 52/88 (58%)
 Frame = -2

Query: 557 DFELVTVSPVVTASLASKSVQFAPVGLVNMLNTGGAIPSLDFDEAQNLVPVGVRGTGEMR 378
           + E+ TVSP+   S     V FAP+GLVNM N+GGAI  L ++  +  V + V+G G+  
Sbjct: 739 EHEIFTVSPI---SHLVDGVSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFG 795

Query: 377 VFSSERPSACRIDGKEADFEYEGSMVII 294
            +SS +P  C ++  E  FEY+ S  ++
Sbjct: 796 SYSSVKPKRCVVESNEIAFEYDSSSGLV 823

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 490,930,809
Number of Sequences: 1393205
Number of extensions: 10896666
Number of successful extensions: 38774
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 37014
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 38717
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19808345223
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MRL002b10_f BP083782 1 373 2 GENLf071e12 BP066190 2 439 3 GENLf051e01 BP065074 3 104 4 GENLf045d10 BP064718 31 439 5 MWM002b11_f AV764672 31 560 6 MPDL078c02_f AV780512 32 560 7 SPDL021b06_f BP053289 39 443

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