Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000915A_C01 KMC000915A_c01
(628 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_181400.1| methylenetetrahydrofolate dehydrogenase; protei... 216 1e-55
ref|NP_191971.1| putative tetrahydrofolate synthase; protein id:... 205 4e-52
ref|NP_191969.1| putative tetrahydrofolate synthase; protein id:... 191 9e-48
pir||T50664 methylenetetrahydrofolate dehydrogenase (NADP) (EC 1... 183 1e-45
gb|AAM62762.1| 5,10-methylenetetrahydrofolate dehydrogenase:5,10... 181 7e-45
>ref|NP_181400.1| methylenetetrahydrofolate dehydrogenase; protein id: At2g38660.1
[Arabidopsis thaliana] gi|25284864|pir||G84807
methylenetetrahydrofolate dehydrogenase [imported] -
Arabidopsis thaliana gi|3786006|gb|AAC67352.1|
methylenetetrahydrofolate dehydrogenase [Arabidopsis
thaliana]
Length = 350
Score = 216 bits (551), Expect = 1e-55
Identities = 106/139 (76%), Positives = 122/139 (87%)
Frame = -1
Query: 628 LIRSGVKIMGKKAVVIGRSNIVGLPASLLLQRHHATVTVVHAFTENPEQITSKADIVVSA 449
LIR+GV+I GK AVVIGRSNIVGLP SLLLQRH ATV+ VHAFT++PE IT KADIV++A
Sbjct: 210 LIRTGVEIAGKNAVVIGRSNIVGLPMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAA 269
Query: 448 AGVPNLVRGNWIKNGATVIDVGTIPVEDPSCEDGYRLVGDVCFEEAVKVASLVTPVPGGV 269
AG+PNLVRG+W+K GA VIDVGT PVED SCE GYRLVGDVC+EEA+ VAS +TPVPGGV
Sbjct: 270 AGIPNLVRGSWLKPGAVVIDVGTTPVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGV 329
Query: 268 GPMTVAMLLLNTLTSAKRM 212
GPMT+ MLL NTL +AKR+
Sbjct: 330 GPMTITMLLCNTLEAAKRI 348
>ref|NP_191971.1| putative tetrahydrofolate synthase; protein id: At4g00620.1,
supported by cDNA: gi_16649148 [Arabidopsis thaliana]
gi|7488134|pir||T01226 probable
methylenetetrahydrofolate dehydrogenase (NADP) (EC
1.5.1.5) F6N23.26 - Arabidopsis thaliana
gi|3047116|gb|AAC13627.1| F6N23.26 gene product
[Arabidopsis thaliana] gi|7267401|emb|CAB80871.1|
putative tetrahydrofolate synthase [Arabidopsis
thaliana] gi|16649149|gb|AAL24426.1| Unknown protein
[Arabidopsis thaliana] gi|23197716|gb|AAN15385.1|
Unknown protein [Arabidopsis thaliana]
Length = 360
Score = 205 bits (521), Expect = 4e-52
Identities = 99/142 (69%), Positives = 121/142 (84%)
Frame = -1
Query: 628 LIRSGVKIMGKKAVVIGRSNIVGLPASLLLQRHHATVTVVHAFTENPEQITSKADIVVSA 449
L R ++I GK+AVVIGRSNIVG+PA+LLLQR ATV+++H+ T+NPE+IT +ADI++SA
Sbjct: 218 LHRYNIEIKGKRAVVIGRSNIVGMPAALLLQREDATVSIIHSRTKNPEEITREADIIISA 277
Query: 448 AGVPNLVRGNWIKNGATVIDVGTIPVEDPSCEDGYRLVGDVCFEEAVKVASLVTPVPGGV 269
G PN+VRG+WIK GA +IDVG PVEDPS GYRLVGD+C+EEA KVAS +TPVPGGV
Sbjct: 278 VGQPNMVRGSWIKPGAVLIDVGINPVEDPSAARGYRLVGDICYEEASKVASAITPVPGGV 337
Query: 268 GPMTVAMLLLNTLTSAKRMLNF 203
GPMT+AMLL NTLTSAKR+ NF
Sbjct: 338 GPMTIAMLLSNTLTSAKRIHNF 359
>ref|NP_191969.1| putative tetrahydrofolate synthase; protein id: At4g00600.1
[Arabidopsis thaliana] gi|7431820|pir||T01224 probable
methylenetetrahydrofolate dehydrogenase (NADP) (EC
1.5.1.5) F6N23.28 - Arabidopsis thaliana
gi|3047121|gb|AAC13632.1| similar to other
dehydrogenase/cyclohydrolase domains [Arabidopsis
thaliana] gi|7267399|emb|CAB80869.1| putative
tetrahydrofolate synthase [Arabidopsis thaliana]
Length = 310
Score = 191 bits (484), Expect = 9e-48
Identities = 92/142 (64%), Positives = 116/142 (80%)
Frame = -1
Query: 628 LIRSGVKIMGKKAVVIGRSNIVGLPASLLLQRHHATVTVVHAFTENPEQITSKADIVVSA 449
L R ++ GK+AVVIGRSNIVG+PA+LLLQ+ ATV+++H+ T NPE++T +ADI++SA
Sbjct: 168 LHRYNIEFKGKRAVVIGRSNIVGMPAALLLQKEDATVSIIHSRTMNPEELTRQADILISA 227
Query: 448 AGVPNLVRGNWIKNGATVIDVGTIPVEDPSCEDGYRLVGDVCFEEAVKVASLVTPVPGGV 269
G PN+VRG+WIK GA +IDVG PVEDPS G RLVGD+C+ EA K+AS +TPVPG V
Sbjct: 228 VGKPNMVRGSWIKPGAVLIDVGIKPVEDPSAAGGERLVGDICYVEASKIASAITPVPGDV 287
Query: 268 GPMTVAMLLLNTLTSAKRMLNF 203
GPMT+AMLL NTLTSAKR+ NF
Sbjct: 288 GPMTIAMLLSNTLTSAKRIHNF 309
>pir||T50664 methylenetetrahydrofolate dehydrogenase (NADP) (EC 1.5.1.5) /
methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)
[imported] - garden pea gi|4103987|gb|AAD01907.1|
5,10-methylenetetrahydrofolate
dehydrogenase-5,10-methenyltetrahydrofolate
cyclohydrolase [Pisum sativum]
gi|6002383|emb|CAB56756.1|
5,10-methylenetetrahydrofolate dehydrogenase:
5,10-methenyltetrahydrofolate cyclohydrolase [Pisum
sativum]
Length = 294
Score = 183 bits (465), Expect = 1e-45
Identities = 94/140 (67%), Positives = 110/140 (78%)
Frame = -1
Query: 628 LIRSGVKIMGKKAVVIGRSNIVGLPASLLLQRHHATVTVVHAFTENPEQITSKADIVVSA 449
L RSGV I GKKAVV+GRSNIVGLPASLLL + ATVT+VH+ T PE I +ADIV++A
Sbjct: 151 LSRSGVSIKGKKAVVVGRSNIVGLPASLLLLKADATVTIVHSHTSQPETIIREADIVIAA 210
Query: 448 AGVPNLVRGNWIKNGATVIDVGTIPVEDPSCEDGYRLVGDVCFEEAVKVASLVTPVPGGV 269
AG +++G+WIK GA VIDVGT V+DP+ + GYRLVGDV FEEA KVA +TPVPGGV
Sbjct: 211 AGQAKMIKGSWIKPGAAVIDVGTNSVDDPTRKSGYRLVGDVDFEEASKVAGWITPVPGGV 270
Query: 268 GPMTVAMLLLNTLTSAKRML 209
GPMTV MLL NTL AKR +
Sbjct: 271 GPMTVTMLLKNTLEGAKRTI 290
>gb|AAM62762.1| 5,10-methylenetetrahydrofolate
dehydrogenase:5,10-methenyltetrahydrofolate
cyclohydrolase, putative [Arabidopsis thaliana]
Length = 299
Score = 181 bits (459), Expect = 7e-45
Identities = 92/139 (66%), Positives = 110/139 (78%)
Frame = -1
Query: 628 LIRSGVKIMGKKAVVIGRSNIVGLPASLLLQRHHATVTVVHAFTENPEQITSKADIVVSA 449
L RSGVKI G++AVV+GRSNIVGLP SLLL + ATVT VH+ T++PE I +ADIV++A
Sbjct: 158 LARSGVKIKGQRAVVVGRSNIVGLPVSLLLLKADATVTTVHSHTKDPEAIIREADIVIAA 217
Query: 448 AGVPNLVRGNWIKNGATVIDVGTIPVEDPSCEDGYRLVGDVCFEEAVKVASLVTPVPGGV 269
G ++++GNWIK GA VIDVGT V DPS + GYRLVGDV F EA KVA +TPVPGGV
Sbjct: 218 CGQAHMIKGNWIKPGAAVIDVGTNAVSDPSKKSGYRLVGDVDFAEASKVAGFITPVPGGV 277
Query: 268 GPMTVAMLLLNTLTSAKRM 212
GPMTVAMLL NT+ AKR+
Sbjct: 278 GPMTVAMLLRNTVDGAKRV 296
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 576,894,483
Number of Sequences: 1393205
Number of extensions: 13011748
Number of successful extensions: 37702
Number of sequences better than 10.0: 261
Number of HSP's better than 10.0 without gapping: 35259
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37388
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25586195130
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
