>KMC000907A_C01 KMC000907A_c01
CTGGTAACATAGGAAACTATATTTAATTAATTAGACCACTGCACTTTATACAAGTCTCTG
ATTAATTAATAATGAGAAGAAAACTAAGTATCCTAATGTAACAAGAATGGGCCATACAAA
AAACATGTGGAGACCCACAAAATCATTTCTCCTAACCACCATATCATAGACCGCACTTTG
AACGCACAATTCATGGGACACTGTCACAACCACTTCCCCTTTTTTTCCTTCCTCATCCGT
ACACGTCAAGATCTAGGACGAAGCACATCGAACGGCCTGTGTGAAATGGGAGCAGTGGAG
GCCCACGCGCCAACCGAGAAGGGACGCGCGTGATTATTAGGAGCCGACATGGGATGATGG
TGGCGCGTGGATGGATCAACCGCGGAGGGCGGAACTGCCACGCGCTCGCAGGATGGGCAC
ATGGTGAGAGTGGTCGGTGGGGTCATCTGCATGTAGAACTGCGGGGAAAGCTTGAGTGCT
CTGAGCTCCTGAACCTCCTTCTGCAACCTTCTGTTCTCCTCCGTCAGATTCTCGC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000907A_C01 KMC000907A_c01
         (535 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T07614 homeobox-leucine zipper protein homolog h1 - soybean...   126  1e-28
ref|NP_193411.1| homeobox-leucine zipper protein HAT4 (HD-Zip pr...   116  2e-25
emb|CAA79670.1| HAT4 [Arabidopsis thaliana] gi|166752|gb|AAA3281...   114  6e-25
emb|CAB96199.1| hypothetical protein [Capsella rubella]               111  5e-24
pir||T52374 homeobox-leucine zipper protein [imported] - Pimpine...   109  2e-23

>pir||T07614 homeobox-leucine zipper protein homolog h1 - soybean
           gi|1234900|emb|CAA63222.1| homeobox-leucine zipper
           protein [Glycine max]
          Length = 284

 Score =  126 bits (317), Expect = 1e-28
 Identities = 63/76 (82%), Positives = 66/76 (85%), Gaps = 1/76 (1%)
 Frame = -3

Query: 533 ENLTEENRRLQKEVQELRALKLSPQFYMQMTPPTTLTMCPSCERVAVPP-SAVDPSTRHH 357
           ENLTEENRRLQKEVQELRALKLSPQFYM MTPPTTLTMCPSCERVAVPP SAVDP+TRHH
Sbjct: 183 ENLTEENRRLQKEVQELRALKLSPQFYMHMTPPTTLTMCPSCERVAVPPSSAVDPATRHH 242

Query: 356 HPMSAPNNHARPFSVG 309
           H    P +H R F +G
Sbjct: 243 H---VPPSHPRAFPIG 255

>ref|NP_193411.1| homeobox-leucine zipper protein HAT4 (HD-Zip protein 4); protein
           id: At4g16780.1, supported by cDNA: gi_14030724,
           supported by cDNA: gi_166751, supported by cDNA:
           gi_19548072 [Arabidopsis thaliana]
           gi|462281|sp|Q05466|HAT4_ARATH Homeobox-leucine zipper
           protein HAT4 (HD-ZIP protein 4) (HD-ZIP protein ATHB-2)
           gi|629516|pir||S31424 DNA-binding homeotic protein
           Athb-2 - Arabidopsis thaliana gi|16180|emb|CAA48246.1|
           Athb-2 [Arabidopsis thaliana] gi|16332|emb|CAA48248.1|
           DNA binding protein [Arabidopsis thaliana]
           gi|2245033|emb|CAB10452.1| DNA-binding homeotic protein
           Athb-2 [Arabidopsis thaliana] gi|7268428|emb|CAB78720.1|
           DNA-binding homeotic protein Athb-2 [Arabidopsis
           thaliana] gi|14030725|gb|AAK53037.1|AF375453_1
           AT4g16780/dl4415w [Arabidopsis thaliana]
           gi|19548073|gb|AAL87400.1| AT4g16780/dl4415w
           [Arabidopsis thaliana]
          Length = 284

 Score =  116 bits (290), Expect = 2e-25
 Identities = 62/96 (64%), Positives = 66/96 (68%), Gaps = 1/96 (1%)
 Frame = -3

Query: 533 ENLTEENRRLQKEVQELRALKLSPQFYMQMTPPTTLTMCPSCERVAVPPSAVDPSTRHHH 354
           ENLTEENRRLQKEV ELRALKLSPQFYM M+PPTTLTMCPSCE V+VPP           
Sbjct: 198 ENLTEENRRLQKEVTELRALKLSPQFYMHMSPPTTLTMCPSCEHVSVPPP---------Q 248

Query: 353 PMSAPNNHARPFSVGAWASTAPISH-RPFDVLRPRS 249
           P +A + H R   V AWA    ISH   FD LRPRS
Sbjct: 249 PQAATSAHHRSLPVNAWAPATRISHGLTFDALRPRS 284

>emb|CAA79670.1| HAT4 [Arabidopsis thaliana] gi|166752|gb|AAA32815.1| homeobox
           protein
          Length = 284

 Score =  114 bits (286), Expect = 6e-25
 Identities = 61/96 (63%), Positives = 65/96 (67%), Gaps = 1/96 (1%)
 Frame = -3

Query: 533 ENLTEENRRLQKEVQELRALKLSPQFYMQMTPPTTLTMCPSCERVAVPPSAVDPSTRHHH 354
           ENLTEENRRLQKEV ELR LKLSPQFYM M+PPTTLTMCPSCE V+VPP           
Sbjct: 198 ENLTEENRRLQKEVTELRVLKLSPQFYMHMSPPTTLTMCPSCEHVSVPPP---------Q 248

Query: 353 PMSAPNNHARPFSVGAWASTAPISH-RPFDVLRPRS 249
           P +A + H R   V AWA    ISH   FD LRPRS
Sbjct: 249 PQAATSAHHRSLPVNAWAPATRISHGLTFDALRPRS 284

>emb|CAB96199.1| hypothetical protein [Capsella rubella]
          Length = 289

 Score =  111 bits (278), Expect = 5e-24
 Identities = 62/99 (62%), Positives = 66/99 (66%), Gaps = 4/99 (4%)
 Frame = -3

Query: 533 ENLTEENRRLQKEVQELRALKLSPQFYMQMTPPTTLTMCPSCERVAVPPSAVDP---STR 363
           ENLTEENRRLQKEV ELRALKLSPQFYM M+PPTTLTMCPSCE V+ PP    P   ++ 
Sbjct: 199 ENLTEENRRLQKEVTELRALKLSPQFYMHMSPPTTLTMCPSCEHVSAPPPQQPPQAATSA 258

Query: 362 HHHPMSAPNNHARPFSVGAWASTAPISH-RPFDVLRPRS 249
            HH  S P        V AWA    ISH   FD LRPRS
Sbjct: 259 QHHRGSLP--------VNAWAQGTRISHGLTFDALRPRS 289

>pir||T52374 homeobox-leucine zipper protein [imported] - Pimpinella brachycarpa
           gi|1149535|emb|CAA64221.1| homeobox-leucine zipper
           protein [Pimpinella brachycarpa]
          Length = 302

 Score =  109 bits (273), Expect = 2e-23
 Identities = 62/98 (63%), Positives = 70/98 (71%), Gaps = 7/98 (7%)
 Frame = -3

Query: 533 ENLTEENRRLQKEVQELRALKLSPQFYMQMTPPTTLTMCPSCERVAVPP-SAVDPST--- 366
           ENLT+ENRRL KEVQELRALKLSPQFYMQMTPPTTLTMCPSCERV+ PP S+  PS+   
Sbjct: 208 ENLTDENRRLMKEVQELRALKLSPQFYMQMTPPTTLTMCPSCERVSAPPSSSTGPSSTPV 267

Query: 365 ---RHHHPMSAPNNHARPFSVGAWASTAPISHRPFDVL 261
              R HH  S  ++H   F+   WA  AP  HR FD +
Sbjct: 268 ETPRPHH--SGSSHHRVAFN--PWA-IAPAGHRSFDAV 300

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 511,760,446
Number of Sequences: 1393205
Number of extensions: 12065428
Number of successful extensions: 36610
Number of sequences better than 10.0: 89
Number of HSP's better than 10.0 without gapping: 34087
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36276
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17885181664
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf018g08 BP068703 1 541 2 MR050e07_f BP079879 31 487 3 GNf033b09 BP069748 31 521 4 GENf022g01 BP059305 33 485 5 GENf009f12 BP058735 35 476 6 MR014g04_f BP077056 36 404 7 MFB001g09_f BP033997 43 284 8 GENLf044b02 BP064657 49 571 9 GENf099d12 BP062363 55 525

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