KMC000899A_c01
[Fasta Sequence]
[Nr Search]
[EST assemble image]
Fasta Sequence
>KMC000899A_C01 KMC000899A_c01
ccctcgagttttttttttttttttttagccaGGAAGAAAACAGTTCCCTGGTATGTAATT
AATCAGAGAGATGTATACAAAACAAAAGGCCAAGGCTGAATCTGCAACAGCAGAACAAAC
AGTAACTGCCAGTAGCATTTAACTGGCCATCAATGTCGCCTTCCTAAATTCTAATATGGA
ATTATGTGACAGAAAACAGGTAGTAATACTTGGTACAGCAACAAAATTTATTGGCTTAAA
GAAAAGACAGCTATTACATTAGATTCTCATTCTTCCATCACAACCACTTAGAAAAACAGA
CAAAAAAACAGGTTTACAAGGAGCAAATACTGGAGAGACACTCCATTCATCAAACCTAAT
TACAGTGGTCACAAAATTCACTCTTCCATCTCACCATCATCAATTTCCTCAATCCTCATC
TTCTTCACAGCTGGAGCAGCATCACTTTTGGGTCCATTAGGTATCTGAATCAAGATATCA
GACTTCTGACCAGCTTGCAGCATTAACCAGCCCACCATTCTTCTCTACACGGTAGACCAA
CTCACTACCCTTGGTGCCAGCATCGCATGTACACTGTTGAGTTCTCATCAATTTCATCCC
TTATAAGCATTCTGGAAAGCTCTGTCACCACTTTCCTCTCAAGCCACCTCCTAATTGGTC
TAGCACCATAAACAGGATCATAGCTCTCAGCAAGTATATAATCTAATGCTGCATCTGTTA
CTGCCATGGCTATTCCTCTCTCAGCAAGTCTACTAGCAAC
Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000899A_C01 KMC000899A_c01
(760 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T07807 endopeptidase Clp (EC 3.4.21.-) ATP-binding chain SB... 120 4e-44
gb|AAC83688.2| 101 kDa heat shock protein; HSP101 [Nicotiana tab... 108 3e-42
gb|AAA67927.1| AtHSP101 117 3e-35
ref|NP_565083.1| heat shock protein 101 (HSP101); protein id: At... 117 3e-35
gb|AAL57165.1|AF332981_1 heat shock protein [Oryza sativa] 105 8e-34
>pir||T07807 endopeptidase Clp (EC 3.4.21.-) ATP-binding chain SB100
[similarity] - soybean gi|530207|gb|AAA66338.1| heat
shock protein
Length = 911
Score = 120 bits (301), Expect(3) = 4e-44
Identities = 58/65 (89%), Positives = 65/65 (99%)
Frame = -1
Query: 760 VASRLAERGIAMAVTDAALDYILAESYDPVYGARPIRRWLERKVVTELSRMLIRDEIDEN 581
VASRLAE+GIA+AVTDAALDYIL+ESYDPVYGARPIRRWLE+KVVTELSRML+R+EIDEN
Sbjct: 784 VASRLAEKGIALAVTDAALDYILSESYDPVYGARPIRRWLEKKVVTELSRMLVREEIDEN 843
Query: 580 STVYM 566
STVY+
Sbjct: 844 STVYI 848
Score = 62.0 bits (149), Expect(3) = 4e-44
Identities = 32/39 (82%), Positives = 34/39 (87%), Gaps = 2/39 (5%)
Frame = -3
Query: 491 GQKSDILIQIPNG--PKSDAAPAVKKMRIEEIDDGEMEE 381
GQKSDILIQIPNG PK+DA AVKKM+IEEIDD EMEE
Sbjct: 873 GQKSDILIQIPNGHAPKTDAVQAVKKMKIEEIDDDEMEE 911
Score = 38.9 bits (89), Expect(3) = 4e-44
Identities = 17/21 (80%), Positives = 18/21 (84%)
Frame = -2
Query: 564 DAGTKGSELVYRVEKNGGLVN 502
DAG G ELVYRVEKNGG+VN
Sbjct: 849 DAGPNGGELVYRVEKNGGVVN 869
>gb|AAC83688.2| 101 kDa heat shock protein; HSP101 [Nicotiana tabacum]
Length = 909
Score = 108 bits (271), Expect(3) = 3e-42
Identities = 53/65 (81%), Positives = 60/65 (91%)
Frame = -1
Query: 760 VASRLAERGIAMAVTDAALDYILAESYDPVYGARPIRRWLERKVVTELSRMLIRDEIDEN 581
VA RLAERGIA+ VT+AA D IL ESYDPVYGARPIRRWLERKVVTELS+ML+++EIDEN
Sbjct: 784 VALRLAERGIALGVTEAAPDVILTESYDPVYGARPIRRWLERKVVTELSKMLVKEEIDEN 843
Query: 580 STVYM 566
STVY+
Sbjct: 844 STVYI 848
Score = 65.1 bits (157), Expect(3) = 3e-42
Identities = 31/44 (70%), Positives = 36/44 (81%)
Frame = -3
Query: 512 GWLMLQAGQKSDILIQIPNGPKSDAAPAVKKMRIEEIDDGEMEE 381
G + GQKSDILIQ+PNGP+SDA AVKKMRIEEI+D EME+
Sbjct: 866 GLVNAATGQKSDILIQLPNGPRSDAVQAVKKMRIEEIEDDEMED 909
Score = 41.2 bits (95), Expect(3) = 3e-42
Identities = 18/24 (75%), Positives = 20/24 (83%)
Frame = -2
Query: 564 DAGTKGSELVYRVEKNGGLVNAAS 493
DAG G +L YRVEKNGGLVNAA+
Sbjct: 849 DAGVSGKDLTYRVEKNGGLVNAAT 872
>gb|AAA67927.1| AtHSP101
Length = 911
Score = 117 bits (292), Expect(2) = 3e-35
Identities = 55/65 (84%), Positives = 64/65 (97%)
Frame = -1
Query: 760 VASRLAERGIAMAVTDAALDYILAESYDPVYGARPIRRWLERKVVTELSRMLIRDEIDEN 581
VA RLAERG+A+AVTDAALDYILAESYDPVYGARPIRRW+E+KVVTELS+M++R+EIDEN
Sbjct: 784 VAVRLAERGVALAVTDAALDYILAESYDPVYGARPIRRWMEKKVVTELSKMVVREEIDEN 843
Query: 580 STVYM 566
STVY+
Sbjct: 844 STVYI 848
Score = 54.3 bits (129), Expect(2) = 3e-35
Identities = 29/45 (64%), Positives = 34/45 (75%), Gaps = 1/45 (2%)
Frame = -3
Query: 512 GWLMLQAGQKSDILIQIPNGPK-SDAAPAVKKMRIEEIDDGEMEE 381
G + G+KSD+LI I NGPK SDAA AVKKMRIEEI+D + EE
Sbjct: 863 GLVDASTGKKSDVLIHIANGPKRSDAAQAVKKMRIEEIEDDDNEE 907
>ref|NP_565083.1| heat shock protein 101 (HSP101); protein id: At1g74310.1, supported
by cDNA: gi_537445 [Arabidopsis thaliana]
gi|21264430|sp|P42730|H101_ARATH Heat shock protein 101
gi|25289887|pir||F96771 heat shock protein 101,
13093-16240 [imported] - Arabidopsis thaliana
gi|6715468|gb|AAF26423.1|AF218796_1 heat shock protein
101 [Arabidopsis thaliana]
gi|12324908|gb|AAG52410.1|AC020579_12 heat shock protein
101; 13093-16240 [Arabidopsis thaliana]
Length = 911
Score = 117 bits (292), Expect(2) = 3e-35
Identities = 55/65 (84%), Positives = 64/65 (97%)
Frame = -1
Query: 760 VASRLAERGIAMAVTDAALDYILAESYDPVYGARPIRRWLERKVVTELSRMLIRDEIDEN 581
VA RLAERG+A+AVTDAALDYILAESYDPVYGARPIRRW+E+KVVTELS+M++R+EIDEN
Sbjct: 784 VAVRLAERGVALAVTDAALDYILAESYDPVYGARPIRRWMEKKVVTELSKMVVREEIDEN 843
Query: 580 STVYM 566
STVY+
Sbjct: 844 STVYI 848
Score = 54.3 bits (129), Expect(2) = 3e-35
Identities = 29/45 (64%), Positives = 34/45 (75%), Gaps = 1/45 (2%)
Frame = -3
Query: 512 GWLMLQAGQKSDILIQIPNGPK-SDAAPAVKKMRIEEIDDGEMEE 381
G + G+KSD+LI I NGPK SDAA AVKKMRIEEI+D + EE
Sbjct: 863 GLVDASTGKKSDVLIHIANGPKRSDAAQAVKKMRIEEIEDDDNEE 907
>gb|AAL57165.1|AF332981_1 heat shock protein [Oryza sativa]
Length = 912
Score = 105 bits (262), Expect(3) = 8e-34
Identities = 50/65 (76%), Positives = 61/65 (92%)
Frame = -1
Query: 760 VASRLAERGIAMAVTDAALDYILAESYDPVYGARPIRRWLERKVVTELSRMLIRDEIDEN 581
VA RLAERG+A+AVTDAALD IL+ SYDPVYGARPIRRW+E++VVT+LS+MLI++EIDEN
Sbjct: 785 VAVRLAERGVALAVTDAALDVILSLSYDPVYGARPIRRWIEKRVVTQLSKMLIQEEIDEN 844
Query: 580 STVYM 566
TVY+
Sbjct: 845 CTVYI 849
Score = 51.6 bits (122), Expect(3) = 8e-34
Identities = 29/46 (63%), Positives = 33/46 (71%), Gaps = 2/46 (4%)
Frame = -3
Query: 512 GWLMLQAGQKSDILIQIPNGPK--SDAAPAVKKMRIEEIDDGEMEE 381
G + + GQKSDILIQ+PNG SDAA AVKKMRI E +DG EE
Sbjct: 867 GLVNAETGQKSDILIQVPNGAATGSDAAQAVKKMRIMEDEDGMDEE 912
Score = 29.6 bits (65), Expect(3) = 8e-34
Identities = 15/29 (51%), Positives = 17/29 (57%), Gaps = 2/29 (6%)
Frame = -2
Query: 579 QQCTC--DAGTKGSELVYRVEKNGGLVNA 499
+ CT DA EL YRV+ GGLVNA
Sbjct: 843 ENCTVYIDAAPHKDELAYRVDNRGGLVNA 871
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 634,731,182
Number of Sequences: 1393205
Number of extensions: 13401293
Number of successful extensions: 35522
Number of sequences better than 10.0: 358
Number of HSP's better than 10.0 without gapping: 34036
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35462
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 37158613404
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
EST assemble image
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clone |
accession |
position |
| 1 |
SPDL003a03_f |
BP052147 |
1 |
463 |
| 2 |
GENLf071e03 |
BP066183 |
32 |
601 |
| 3 |
SPDL092b03_f |
BP057751 |
32 |
685 |
| 4 |
MRL026d08_f |
BP085050 |
191 |
712 |
| 5 |
SPDL025g03_f |
BP053568 |
201 |
785 |
| 6 |
SPDL054f06_f |
BP055419 |
202 |
801 |
| 7 |
GENLf048a12 |
BP064878 |
208 |
791 |
| 8 |
GENLf083f11 |
BP066888 |
208 |
690 |
| 9 |
MRL036e04_f |
BP085489 |
210 |
384 |
| 10 |
GENLf049f07 |
BP064973 |
212 |
683 |
| 11 |
MRL001d02_f |
BP083723 |
213 |
840 |
| 12 |
MPDL009c09_f |
AV776969 |
213 |
366 |
| 13 |
MRL033a08_f |
BP085328 |
215 |
653 |
| 14 |
MPDL020f02_f |
AV777519 |
215 |
592 |
| 15 |
SPDL028d06_f |
BP053734 |
215 |
808 |
| 16 |
GENLf043e10 |
BP064625 |
215 |
791 |
| 17 |
MPDL016c01_f |
AV777299 |
216 |
785 |
| 18 |
MRL044e10_f |
BP085868 |
216 |
364 |
| 19 |
MRL005h02_f |
BP083979 |
216 |
720 |
| 20 |
SPDL076c02_f |
BP056691 |
217 |
788 |
| 21 |
GENLf090h02 |
BP067290 |
218 |
787 |
| 22 |
MRL011e03_f |
BP084265 |
218 |
674 |
| 23 |
MRL029e01_f |
BP085170 |
234 |
462 |
| 24 |
SPDL069b09_f |
BP056262 |
234 |
823 |
| 25 |
SPDL060c08_f |
BP055738 |
234 |
812 |
| 26 |
MRL028f05_f |
BP085141 |
235 |
732 |
| 27 |
SPDL050f10_f |
BP055168 |
238 |
682 |
|
Lotus japonicus
Kazusa DNA Research Institute