>KMC000884A_C01 KMC000884A_c01
tcgaaatggtatgcatgaatgaaatatgaaatatgctacaacacaagatACCAAATTACA
AACACATATACTAGCAAATGTAACAATTATGTCCACTGGAAACTTTTTACATGGAGTATG
TGCATATAAGCAGAGGGACATGAAACACAATTCTAGCCACAAGACAACAAAAAGCTAATG
TAAATATAATATATAAACTATAAAGCAAGTATTCACCAGCAAGTGAGGGAAGGATAAAAA
CTATTTCTTCCCACAGCCACCAACCCTTCTAGAAGTGGTAAGAAACATCTCAACCCCACA
ACAGAAACAACACCAAAGGTGATACTGATAAATGGTGAGGACGCTGGAATCTCCAAGAAG
CAGTGAACATATAAACTAACATGAAGAACTTCTTTGTATTATCCATGCGAATCTACCGAA
AATGCAGGGACCACACATCTCTCTTTTCTTGATGCTGAAAATGGCATCACTCCCAACATT
AAGATGATGTCTTTCATGCGCTGACGATAATTAAATTAGTATATGGCATACACTATCTCC
CCACTGCTATCTATATCAAGCTCCGATTCAGAATCCAATTCGTCAAAGTCGTCATCCATG
TCAATGTTATCCATCATAAACTTGTCTAAAGAACAGGCCCCAG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000884A_C01 KMC000884A_c01
         (643 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAM13097.1| putative protein [Arabidopsis thaliana] gi|250835...    62  6e-09
ref|NP_201489.1| protein kinase, 41K (EC 2.7.1.-) (pir||S71172);...    62  6e-09
ref|NP_190619.1| protein kinase SPK-2; protein id: At3g50500.1, ...    62  8e-09
gb|AAM65501.1| protein kinase SPK-2 [Arabidopsis thaliana]             62  8e-09
gb|AAF27340.1|AF186020_1 abscisic acid-activated protein kinase ...    47  2e-04

>gb|AAM13097.1| putative protein [Arabidopsis thaliana] gi|25083584|gb|AAN72093.1|
           putative protein [Arabidopsis thaliana]
          Length = 349

 Score = 62.0 bits (149), Expect = 6e-09
 Identities = 27/36 (75%), Positives = 32/36 (88%)
 Frame = -3

Query: 629 LDKFMMDNIDMDDDFDELDSESELDIDSSGEIVYAI 522
           LD FM DN+D+DDD D+ DSESE+DIDSSGEIVYA+
Sbjct: 314 LDDFMTDNLDLDDDMDDFDSESEIDIDSSGEIVYAL 349

>ref|NP_201489.1| protein kinase, 41K (EC 2.7.1.-) (pir||S71172); protein id:
           At5g66880.1, supported by cDNA: gi_166818 [Arabidopsis
           thaliana] gi|2146746|pir||S71172 protein kinase, 41K (EC
           2.7.1.-) - Arabidopsis thaliana gi|166819|gb|AAA32846.1|
           protein kinase gi|9758138|dbj|BAB08630.1| protein
           kinase, 41K (EC 2.7.1.-) [Arabidopsis thaliana]
          Length = 361

 Score = 62.0 bits (149), Expect = 6e-09
 Identities = 27/36 (75%), Positives = 32/36 (88%)
 Frame = -3

Query: 629 LDKFMMDNIDMDDDFDELDSESELDIDSSGEIVYAI 522
           LD FM DN+D+DDD D+ DSESE+DIDSSGEIVYA+
Sbjct: 326 LDDFMTDNLDLDDDMDDFDSESEIDIDSSGEIVYAL 361

>ref|NP_190619.1| protein kinase SPK-2; protein id: At3g50500.1, supported by cDNA:
           3988., supported by cDNA: gi_16209651, supported by
           cDNA: gi_166816 [Arabidopsis thaliana]
           gi|1362002|pir||S56718 protein kinase SPK-2 (EC 2.7.1.-)
           - Arabidopsis thaliana gi|166817|gb|AAA32845.1| protein
           kinase gi|6561990|emb|CAB62479.1| protein kinase SPK-2
           [Arabidopsis thaliana]
          Length = 362

 Score = 61.6 bits (148), Expect = 8e-09
 Identities = 26/36 (72%), Positives = 32/36 (88%)
 Frame = -3

Query: 629 LDKFMMDNIDMDDDFDELDSESELDIDSSGEIVYAI 522
           LD FM DN+D+DDD D+ DSESE+D+DSSGEIVYA+
Sbjct: 327 LDDFMADNLDLDDDMDDFDSESEIDVDSSGEIVYAL 362

>gb|AAM65501.1| protein kinase SPK-2 [Arabidopsis thaliana]
          Length = 362

 Score = 61.6 bits (148), Expect = 8e-09
 Identities = 26/36 (72%), Positives = 32/36 (88%)
 Frame = -3

Query: 629 LDKFMMDNIDMDDDFDELDSESELDIDSSGEIVYAI 522
           LD FM DN+D+DDD D+ DSESE+D+DSSGEIVYA+
Sbjct: 327 LDDFMADNLDLDDDMDDFDSESEIDVDSSGEIVYAL 362

>gb|AAF27340.1|AF186020_1 abscisic acid-activated protein kinase [Vicia faba]
          Length = 349

 Score = 47.4 bits (111), Expect = 2e-04
 Identities = 23/40 (57%), Positives = 31/40 (77%)
 Frame = -3

Query: 641 GACSLDKFMMDNIDMDDDFDELDSESELDIDSSGEIVYAI 522
           G+   D+F    I++D+D DE+DS+ ELD+DSSGEIVYAI
Sbjct: 314 GSYYFDEF----IEVDEDMDEIDSDYELDVDSSGEIVYAI 349

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 562,437,548
Number of Sequences: 1393205
Number of extensions: 12246064
Number of successful extensions: 36746
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 29303
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33757
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 27007650415
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MWM066a11_f AV765758 1 553 2 GENf062e12 BP060999 50 528 3 GENf051h01 BP060542 50 520 4 GENf034h12 BP059824 50 423 5 GENf011b08 BP058802 51 488 6 GENf073a06 BP061458 58 372 7 SPD045g12_f BP047618 58 604 8 GENf040b10 BP060054 58 562 9 GENLf057e08 BP065403 58 604 10 GENf067g07 BP061241 58 412 11 GNf042f04 BP070474 58 594 12 GNf001h03 BP067474 63 497 13 GNf017e07 BP068610 65 548 14 GNf091e01 BP074088 70 164 15 GENLf042e02 BP064564 70 222 16 GENf011d03 BP058810 70 452 17 MFB024b03_f BP035710 71 583 18 GNf083e12 BP073499 185 661

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