Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000742A_C02 KMC000742A_c02
(726 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAB01075.1| gb|AAF35409.1~gene_id:MPE11.29~similar to unknow... 186 2e-46
ref|NP_566789.1| expressed protein; protein id: At3g26100.1, sup... 186 2e-46
gb|AAM45266.1| hypothetical protein [Dictyostelium discoideum] g... 52 1e-05
ref|XP_236362.1| similar to guanine nucleotide exchange factor p... 50 3e-05
dbj|BAC57847.1| OSJNBa0040K22.11 [Oryza sativa (japonica cultiva... 50 4e-05
>dbj|BAB01075.1| gb|AAF35409.1~gene_id:MPE11.29~similar to unknown protein
[Arabidopsis thaliana]
Length = 532
Score = 186 bits (473), Expect = 2e-46
Identities = 91/112 (81%), Positives = 101/112 (89%)
Frame = -3
Query: 724 DYTTFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQIS 545
DYTTF+VSDDGDVYSFGCGESASLGH+ D QG+RHANVLSP +VTSLKQ+NER+VQIS
Sbjct: 420 DYTTFVVSDDGDVYSFGCGESASLGHHPSFDEQGNRHANVLSPTVVTSLKQVNERMVQIS 479
Query: 544 LTNSIYWNAHTFALTESGKLYAFGAGDKGQLGIELVENQTERGNPERVDIGL 389
LTNSIYWNAHTFALTESGKL+AFGAGD+GQLG EL +NQ ER PE+VDI L
Sbjct: 480 LTNSIYWNAHTFALTESGKLFAFGAGDQGQLGTELGKNQKERCVPEKVDIDL 531
Score = 48.9 bits (115), Expect = 7e-05
Identities = 41/103 (39%), Positives = 54/103 (51%)
Frame = -3
Query: 715 TFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLTN 536
T L+SDDG VY+ CG+ S G + G V +PQLVTSLK I VVQ ++ N
Sbjct: 207 TMLISDDGKVYA--CGKE-SFGEA---EYGGQGTKPVTTPQLVTSLKNIF--VVQAAIGN 258
Query: 535 SIYWNAHTFALTESGKLYAFGAGDKGQLGIELVENQTERGNPE 407
T L+ GK+Y F G+ G+LG +QTE + E
Sbjct: 259 YF-----TAVLSREGKVYTFSWGNDGRLG-----HQTEAADVE 291
>ref|NP_566789.1| expressed protein; protein id: At3g26100.1, supported by cDNA:
gi_14334715, supported by cDNA: gi_16323433 [Arabidopsis
thaliana] gi|14334716|gb|AAK59536.1| unknown protein
[Arabidopsis thaliana] gi|16323434|gb|AAL15211.1|
unknown protein [Arabidopsis thaliana]
Length = 432
Score = 186 bits (473), Expect = 2e-46
Identities = 91/112 (81%), Positives = 101/112 (89%)
Frame = -3
Query: 724 DYTTFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQIS 545
DYTTF+VSDDGDVYSFGCGESASLGH+ D QG+RHANVLSP +VTSLKQ+NER+VQIS
Sbjct: 320 DYTTFVVSDDGDVYSFGCGESASLGHHPSFDEQGNRHANVLSPTVVTSLKQVNERMVQIS 379
Query: 544 LTNSIYWNAHTFALTESGKLYAFGAGDKGQLGIELVENQTERGNPERVDIGL 389
LTNSIYWNAHTFALTESGKL+AFGAGD+GQLG EL +NQ ER PE+VDI L
Sbjct: 380 LTNSIYWNAHTFALTESGKLFAFGAGDQGQLGTELGKNQKERCVPEKVDIDL 431
Score = 48.9 bits (115), Expect = 7e-05
Identities = 41/103 (39%), Positives = 54/103 (51%)
Frame = -3
Query: 715 TFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLTN 536
T L+SDDG VY+ CG+ S G + G V +PQLVTSLK I VVQ ++ N
Sbjct: 107 TMLISDDGKVYA--CGKE-SFGEA---EYGGQGTKPVTTPQLVTSLKNIF--VVQAAIGN 158
Query: 535 SIYWNAHTFALTESGKLYAFGAGDKGQLGIELVENQTERGNPE 407
T L+ GK+Y F G+ G+LG +QTE + E
Sbjct: 159 YF-----TAVLSREGKVYTFSWGNDGRLG-----HQTEAADVE 191
>gb|AAM45266.1| hypothetical protein [Dictyostelium discoideum]
gi|28829520|gb|AAM33709.2| similar to Homo sapiens
(Human). HERC2 protein [Dictyostelium discoideum]
Length = 849
Score = 51.6 bits (122), Expect = 1e-05
Identities = 29/86 (33%), Positives = 47/86 (53%)
Frame = -3
Query: 706 VSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLTNSIY 527
++D G+VYS+G G LGH +V+ + H P+ + ++NER+ Q++ ++
Sbjct: 279 ITDQGEVYSWGLGVFGQLGH---GNVKSYLH-----PKKIQQFVELNERIAQVACGSNF- 329
Query: 526 WNAHTFALTESGKLYAFGAGDKGQLG 449
T + G LYAFG G+ GQLG
Sbjct: 330 ----TMVRSVQGLLYAFGHGEYGQLG 351
Score = 39.3 bits (90), Expect = 0.057
Identities = 27/78 (34%), Positives = 42/78 (53%)
Frame = -3
Query: 706 VSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLTNSIY 527
VS GDVYS+G + LG G + V P L+ SL+ +ER+++++ N
Sbjct: 49 VSRFGDVYSWGRSKEGQLGIGQGYG-GSDKVMFVAKPTLIKSLQ--HERIIKVACGNF-- 103
Query: 526 WNAHTFALTESGKLYAFG 473
H+ ALT+ GK+Y +G
Sbjct: 104 ---HSLALTDMGKVYEWG 118
Score = 34.7 bits (78), Expect = 1.4
Identities = 22/81 (27%), Positives = 42/81 (51%)
Frame = -3
Query: 688 VYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLTNSIYWNAHTF 509
V S G ++ LGH++ + L P ++ L + +R++Q ++ AH+
Sbjct: 3 VLSVGQSKNGQLGHDSIKEC--------LEPTVIEGL--LGKRIIQ-----TVSGEAHSL 47
Query: 508 ALTESGKLYAFGAGDKGQLGI 446
A++ G +Y++G +GQLGI
Sbjct: 48 AVSRFGDVYSWGRSKEGQLGI 68
Score = 33.9 bits (76), Expect = 2.4
Identities = 20/53 (37%), Positives = 32/53 (59%)
Frame = -3
Query: 604 LSPQLVTSLKQINERVVQISLTNSIYWNAHTFALTESGKLYAFGAGDKGQLGI 446
L+ + ++ I E VV +S W A + A+T+SG+LY +G +KGQLG+
Sbjct: 199 LNQTIPMQIQDIPEEVVDVSGG----W-AFSAAVTKSGRLYTWGFNEKGQLGL 246
Score = 33.1 bits (74), Expect = 4.1
Identities = 27/94 (28%), Positives = 40/94 (41%), Gaps = 4/94 (4%)
Frame = -3
Query: 715 TFLVSDDGDVYSFGCGESASLGHNAGN---DVQGH-RHANVLSPQLVTSLKQINERVVQI 548
T + S G +Y+FG GE LG D G H P +V SL+
Sbjct: 330 TMVRSVQGLLYAFGHGEYGQLGSTEETQHLDFGGRDNHFKYSIPIVVKSLETKK------ 383
Query: 547 SLTNSIYWNAHTFALTESGKLYAFGAGDKGQLGI 446
+ N + HT +T+ ++Y +G G G LG+
Sbjct: 384 -IKNVACGHLHTIVVTDENEVYQWGWGSSGALGL 416
>ref|XP_236362.1| similar to guanine nucleotide exchange factor p532 [Homo sapiens]
[Rattus norvegicus]
Length = 4237
Score = 50.1 bits (118), Expect = 3e-05
Identities = 36/114 (31%), Positives = 63/114 (54%), Gaps = 1/114 (0%)
Frame = -3
Query: 724 DYTTFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQIS 545
D + +++ G+V+S+G G+ LGH GN + R P+ + +L+ E VVQ++
Sbjct: 3380 DGHSMALTESGEVFSWGDGDYGKLGH--GNSDRQRR------PRQIEALQ--GEEVVQVA 3429
Query: 544 LTNSIYWNAHTFALTESGKL-YAFGAGDKGQLGIELVENQTERGNPERVDIGLG 386
+ HT A++ G + +AFG GD G+LG+ N T + +P++VD+ G
Sbjct: 3430 CGLN-----HTLAVSADGSMVWAFGDGDYGKLGL---GNSTAKSSPQKVDVLCG 3475
Score = 44.3 bits (103), Expect = 0.002
Identities = 29/87 (33%), Positives = 48/87 (54%), Gaps = 1/87 (1%)
Frame = -3
Query: 706 VSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLTNSIY 527
V++DG++Y++G G+ LGH N N+ P LV + + E +S +S
Sbjct: 181 VTEDGELYTWGEGDFGRLGHGDSNS------RNI--PTLVKDISNVGE----VSCGSS-- 226
Query: 526 WNAHTFALTESGK-LYAFGAGDKGQLG 449
HT AL++ G+ +++FG GD G+LG
Sbjct: 227 ---HTIALSKDGRTVWSFGGGDNGKLG 250
Score = 41.2 bits (95), Expect = 0.015
Identities = 27/89 (30%), Positives = 43/89 (47%)
Frame = -3
Query: 715 TFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLTN 536
TF++ +G V + G G LG +D+ + L +VT L
Sbjct: 3328 TFVIQANGTVLACGEGSYGRLGQGNSDDLHVLTVISALQGFVVTQL------------VT 3375
Query: 535 SIYWNAHTFALTESGKLYAFGAGDKGQLG 449
S + H+ ALTESG+++++G GD G+LG
Sbjct: 3376 SCGSDGHSMALTESGEVFSWGDGDYGKLG 3404
Score = 40.0 bits (92), Expect = 0.033
Identities = 31/92 (33%), Positives = 47/92 (50%)
Frame = -3
Query: 724 DYTTFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQIS 545
D T + +G+V+S+G G+ LGH GN + P+L+ Q + VV +S
Sbjct: 122 DGHTLAFTTEGEVFSWGDGDYGKLGH--GNS------STQKYPKLIQGPLQ-GKVVVCVS 172
Query: 544 LTNSIYWNAHTFALTESGKLYAFGAGDKGQLG 449
H+ A+TE G+LY +G GD G+LG
Sbjct: 173 A-----GYRHSAAVTEDGELYTWGEGDFGRLG 199
Score = 39.3 bits (90), Expect = 0.057
Identities = 28/91 (30%), Positives = 44/91 (47%)
Frame = -3
Query: 721 YTTFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISL 542
Y TF++S DG V + G G LG N+ Q P S+K++
Sbjct: 68 YCTFVISTDGSVRACGKGSYGRLGLGDSNN-QSTLKKLTFEPH--RSIKKV--------- 115
Query: 541 TNSIYWNAHTFALTESGKLYAFGAGDKGQLG 449
++S + HT A T G+++++G GD G+LG
Sbjct: 116 SSSKGSDGHTLAFTTEGEVFSWGDGDYGKLG 146
Score = 38.9 bits (89), Expect = 0.074
Identities = 28/90 (31%), Positives = 44/90 (48%)
Frame = -3
Query: 718 TTFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLT 539
++ ++ G VY++GCG A LG G A L P+L+ L R+V IS+
Sbjct: 280 SSLALTSTGQVYAWGCG--ACLG-------CGSSEATALRPKLIEELAA--TRIVDISIG 328
Query: 538 NSIYWNAHTFALTESGKLYAFGAGDKGQLG 449
+S H +L+ ++YA+G GQ G
Sbjct: 329 DS-----HCLSLSHDNEVYAWGNNSMGQCG 353
Score = 37.4 bits (85), Expect = 0.22
Identities = 26/85 (30%), Positives = 42/85 (48%)
Frame = -3
Query: 721 YTTFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISL 542
+T L D V+SFG G++ LGH N V P+++ +L+ + R V
Sbjct: 227 HTIALSKDGRTVWSFGGGDNGKLGHGDTN--------RVYKPKVIEALQGMFIRKVCAGS 278
Query: 541 TNSIYWNAHTFALTESGKLYAFGAG 467
+S+ ALT +G++YA+G G
Sbjct: 279 QSSL-------ALTSTGQVYAWGCG 296
Score = 33.9 bits (76), Expect = 2.4
Identities = 26/87 (29%), Positives = 40/87 (45%)
Frame = -3
Query: 706 VSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLTNSIY 527
++ DG VY+FG + +G G R N PQ + L + V +
Sbjct: 3491 LTKDGHVYTFG--QDRLIGLPEG------RARNHNRPQQIPVLAGVVIEDVAVGAE---- 3538
Query: 526 WNAHTFALTESGKLYAFGAGDKGQLGI 446
HT AL +G +YA+G+ +GQLG+
Sbjct: 3539 ---HTLALASTGDVYAWGSNSEGQLGL 3562
>dbj|BAC57847.1| OSJNBa0040K22.11 [Oryza sativa (japonica cultivar-group)]
Length = 484
Score = 49.7 bits (117), Expect = 4e-05
Identities = 34/89 (38%), Positives = 46/89 (51%)
Frame = -3
Query: 715 TFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLTN 536
TF V+DDG VYSFG + LGH G +H + L P+ + S K+ N VV++S +
Sbjct: 273 TFAVTDDGTVYSFGSCTNFCLGH-------GDQH-DELRPRAIQSFKRRNIHVVRVSAGD 324
Query: 535 SIYWNAHTFALTESGKLYAFGAGDKGQLG 449
H AL G +Y +G G G LG
Sbjct: 325 E-----HAVALDALGYVYTWGRGYCGALG 348
Score = 44.7 bits (104), Expect = 0.001
Identities = 37/118 (31%), Positives = 56/118 (47%)
Frame = -3
Query: 694 GDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLTNSIYWNAH 515
G VY++G G +LGH ND SP+L++SLK ++ VQ+
Sbjct: 334 GYVYTWGRGYCGALGHGDEND--------KTSPELISSLK--SQVAVQVCAR-----KRK 378
Query: 514 TFALTESGKLYAFGAGDKGQLGIELVENQTERGNPERVDIGLG*LFLLPLLLNSLHQH 341
TF LT+ G ++AFG G LG +RG+ ++V + P +L+SL H
Sbjct: 379 TFVLTDEGSVFAFGWMGFGSLGF------PDRGSSDKV--------MRPRVLDSLRDH 422
Score = 41.6 bits (96), Expect = 0.011
Identities = 30/93 (32%), Positives = 49/93 (52%)
Frame = -3
Query: 715 TFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLTN 536
TF+++D+G V++FG SLG V+ P+++ SL+ + V QIS
Sbjct: 379 TFVLTDEGSVFAFGWMGFGSLGFPDRGSSD-----KVMRPRVLDSLR--DHYVSQIS--T 429
Query: 535 SIYWNAHTFALTESGKLYAFGAGDKGQLGIELV 437
+Y HT A+T G ++ FG ++ QLG E +
Sbjct: 430 GLY---HTVAVTNRGIVFGFGDNERAQLGQEYI 459
Score = 33.9 bits (76), Expect = 2.4
Identities = 24/82 (29%), Positives = 41/82 (49%)
Frame = -3
Query: 715 TFLVSDDGDVYSFGCGESASLGHNAGNDVQGHRHANVLSPQLVTSLKQINERVVQISLTN 536
T +++ DG VY+ G LGH D +P++V + + VVQ++
Sbjct: 220 TVILTTDGQVYTCGSNTHGQLGHGDTIDRA--------TPKIVELFEGLAP-VVQVAAGA 270
Query: 535 SIYWNAHTFALTESGKLYAFGA 470
S +TFA+T+ G +Y+FG+
Sbjct: 271 S-----YTFAVTDDGTVYSFGS 287
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 622,582,525
Number of Sequences: 1393205
Number of extensions: 13388640
Number of successful extensions: 35485
Number of sequences better than 10.0: 219
Number of HSP's better than 10.0 without gapping: 32615
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35051
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 34062062287
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
