KMC000741A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC000741A_C01 KMC000741A_c01
acaacaaatatatgtttattaaaaaaggGAATAGAAAAGTTTGGGCATTTATTAAAACAT
AATAACTAGCCACTCCAATATCACCGTTAGAGTACTAAAATCCCAGGGCATGTTAGAATG
CAACACAGCCGCCATGCAGTTCATAATATAGGTAACTTCAGTTCTTTCGAACCATCCACT
TCAAATTTAAATGCACAAATAGGTTTGTTTCACGGCTTCTTCAATCCAATTTTGAATTCA
AATATAAATCTAGATAGAGCTACAGAGCTATATCAGGTCTTGAATATGCTAATTTTCAAG
TCGCCATTAAAATGCAGCACAGGAAATGCATGTTCAGTAGTTGTCTCCAGATTTATTAGT
TCAGCTGCGTACAAATTCCACCTGTCGATCAGAACTATCATTAGAATATAAAGAAACTTT
nGAAACAGAGAAATAGTAAATCCATAATCGACAACTTGAATGGCTAGGAGTTATACTTAA
GCCAAATGAGATTGTTACGGACTC


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000741A_C01 KMC000741A_c01
         (504 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|XP_172440.1| similar to hematopoietic-specific IL-2 deubiqui...    33  1.4
ref|NP_705165.1| hypothetical malaria antigen [Plasmodium falcip...    33  2.4
ref|NP_125918.1| Glucosamine--fructose-6-phosphate aminotransfer...    33  2.4
sp|P59158|S123_MOUSE Solute carrier family 12 member 3 (Thiazide...    32  3.1
sp|P55018|S123_RAT Solute carrier family 12 member 3 (Thiazide-s...    32  3.1

>ref|XP_172440.1| similar to hematopoietic-specific IL-2 deubiquitinating enzyme [Mus
           musculus] [Homo sapiens]
          Length = 489

 Score = 33.5 bits (75), Expect = 1.4
 Identities = 25/114 (21%), Positives = 45/114 (38%), Gaps = 13/114 (11%)
 Frame = +1

Query: 124 HSRHAVHNIGNFSSFEPSTSNLN--------AQIGLFH-----GFFNPILNSNINLDRAT 264
           H   A+HN G+       T+ ++        +QI   H       F+P L+  +++  A 
Sbjct: 131 HITRALHNPGHVDHHSKDTTLIHQIFGGYWRSQIKCLHCHRISDTFDPYLDIALDIQAAQ 190

Query: 265 ELYQVLNMLIFKSPLKCSTGNACSVVVSRFISSAAYKFHLSIRTIIRI*RNFXN 426
            + Q L  L+    L       C V + R  +S     H S + +I + + F +
Sbjct: 191 SVQQALEQLVKPEELNGENAYHCGVCLQRAPASKTLTLHTSAKVLILVLKRFSD 244

>ref|NP_705165.1| hypothetical malaria antigen [Plasmodium falciparum 3D7]
            gi|23615410|emb|CAD52401.1| hypothetical malaria antigen
            [Plasmodium falciparum 3D7]
          Length = 3519

 Score = 32.7 bits (73), Expect = 2.4
 Identities = 24/85 (28%), Positives = 43/85 (50%), Gaps = 1/85 (1%)
 Frame = +1

Query: 130  RHAVHNIGNFSSFEPSTSNLNAQIGLFHGFFNPILNSNINLDRA-TELYQVLNMLIFKSP 306
            +H +H+I NF + E +TS LN  + LF   F   ++  +N+ +A  +L ++  M +    
Sbjct: 2757 KHVLHDINNFYNNEENTSFLNVYVHLF-DIFKKSVDKMLNIIQAQKDLDKIYKMFVEIYY 2815

Query: 307  LKCSTGNACSVVVSRFISSAAYKFH 381
            +K          + RFI  + +KFH
Sbjct: 2816 MK---------KIIRFIIFSFFKFH 2831

>ref|NP_125918.1| Glucosamine--fructose-6-phosphate aminotransferase [Pyrococcus
           abyssi] gi|14548067|sp|Q9V249|GLMS_PYRAB
           Glucosamine--fructose-6-phosphate aminotransferase
           [isomerizing] (Hexosephosphate aminotransferase)
           (D-fructose-6-phosphate amidotransferase) (GFAT)
           (L-glutamine-D-fructose-6-phosphate amidotransferase)
           (Glucosamine-6-phosphate synthase)
           gi|7434163|pir||F75212 glutamine-fructose-6-phosphate
           transaminase (isomerizing)  (EC 2.6.1.16) PAB2201 -
           Pyrococcus abyssi (strain Orsay)
           gi|5457658|emb|CAB49149.1| glmS
           Glucosamine--fructose-6-phosphate aminotransferase
           [isomerizing] [Pyrococcus abyssi]
          Length = 598

 Score = 32.7 bits (73), Expect = 2.4
 Identities = 19/55 (34%), Positives = 27/55 (48%)
 Frame = +1

Query: 16  LLKKGIEKFGHLLKHNN*PLQYHR*STKIPGHVRMQHSRHAVHNIGNFSSFEPST 180
           L+KKG  K   L K  N         T +PG++ + H+R A H I N ++  P T
Sbjct: 43  LIKKGAGKIDELAKRLN--------FTDLPGNIGIGHTRWATHGIPNDTNAHPHT 89

>sp|P59158|S123_MOUSE Solute carrier family 12 member 3 (Thiazide-sensitive
           sodium-chloride cotransporter) (Na-Cl symporter)
           gi|24047306|gb|AAH38612.1| solute carrier family 12,
           member 3 [Mus musculus] gi|26342883|dbj|BAC35098.1|
           unnamed protein product [Mus musculus]
          Length = 1002

 Score = 32.3 bits (72), Expect = 3.1
 Identities = 10/40 (25%), Positives = 22/40 (55%)
 Frame = -1

Query: 177 GWFERTEVTYIMNCMAAVLHSNMPWDFSTLTVILEWLVIM 58
           GW +   +  ++N    +L+  +PW  +   ++L WL+I+
Sbjct: 135 GWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIIL 174

>sp|P55018|S123_RAT Solute carrier family 12 member 3 (Thiazide-sensitive
           sodium-chloride cotransporter) (Na-Cl symporter)
           gi|22389599|gb|AAA21252.2| thiazide-sensitive
           sodium-chloride cotransporter [Rattus norvegicus]
          Length = 1002

 Score = 32.3 bits (72), Expect = 3.1
 Identities = 10/40 (25%), Positives = 22/40 (55%)
 Frame = -1

Query: 177 GWFERTEVTYIMNCMAAVLHSNMPWDFSTLTVILEWLVIM 58
           GW +   +  ++N    +L+  +PW  +   ++L WL+I+
Sbjct: 135 GWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIIL 174

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 388,945,014
Number of Sequences: 1393205
Number of extensions: 7422773
Number of successful extensions: 13706
Number of sequences better than 10.0: 35
Number of HSP's better than 10.0 without gapping: 13443
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 13702
length of database: 448,689,247
effective HSP length: 114
effective length of database: 289,863,877
effective search space used: 15362785481
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 GENLf086e01 BP067038 1 511
2 GNf095f06 BP074424 29 393
3 GENLf033e10 BP064082 33 258
4 GENf091b05 BP062156 33 494




Lotus japonicus
Kazusa DNA Research Institute