Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000653A_C10 KMC000653A_c10
(1238 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAL36933.1|AF213481_1 gamma-tocopherol methyltransferase [Per... 342 2e-98
ref|NP_176677.1| gamma-tocopherol methyltransferase; protein id:... 339 7e-98
sp|Q9ZSK1|GTOM_ARATH Tocopherol O-methyltransferase, chloroplast... 338 2e-97
gb|AAO13806.1| gamma-tocopherol methyltransferase [Brassica oler... 340 2e-97
gb|AAM64696.1| gamma-tocopherol methyltransferase [Arabidopsis t... 336 4e-97
>gb|AAL36933.1|AF213481_1 gamma-tocopherol methyltransferase [Perilla frutescens]
Length = 369
Score = 342 bits (876), Expect(3) = 2e-98
Identities = 179/266 (67%), Positives = 211/266 (79%), Gaps = 4/266 (1%)
Frame = +2
Query: 89 TIRVQSTTQFTGFSIRTRTRDCSRILLTEEREMAVME-EKKLLQTGIAEFYDESSGLWED 265
T + +S T F+ S+RTR R R ++ A ME E + L+ GIAEFYDESSG+WE+
Sbjct: 39 TTKTKSITSFSTDSLRTRGR-ARRPTMSLNAAAAEMETEMETLRKGIAEFYDESSGVWEN 97
Query: 266 MWGDHMHHGFYEQDVTVSVSDHRVAQIRMIEESLRFAALSE-DPAKKPESIVDVGCGIGG 442
+WGDHMHHGFYE VS+SDHR AQIRMIEESLRFA+ S +KP++IVDVGCGIGG
Sbjct: 98 IWGDHMHHGFYEPAADVSISDHRAAQIRMIEESLRFASFSPITTTEKPKNIVDVGCGIGG 157
Query: 443 SSRYLAKKFQAK-SVGITLS-PVQAQRANALAASQGLADKVSFQVADALEQPFPDGQFDL 616
SSRYLA+K+ AK S ITLS PVQAQRA LA +QGL KVSF+VADAL QPFP+G+FDL
Sbjct: 158 SSRYLARKYGAKLSRAITLSSPVQAQRAQQLADAQGLNGKVSFEVADALNQPFPEGKFDL 217
Query: 617 VWSMESGEHMPDKPKFVGELARVAAPGGTIIIVTWCHRDLGPAEESLQPWEQNLLKRICD 796
VWSMESGEHMPDK KFV EL RVAAPGG IIIVTWCHRDL P+EESL+ E++LL +IC
Sbjct: 218 VWSMESGEHMPDKKKFVNELVRVAAPGGRIIIVTWCHRDLSPSEESLRQEEKDLLNKICS 277
Query: 797 AFYLPAWCSTADYVKLLESHSLQE*K 874
A+YLPAWCSTADYVKLL+S S+++ K
Sbjct: 278 AYYLPAWCSTADYVKLLDSLSMEDIK 303
Score = 37.7 bits (86), Expect(3) = 2e-98
Identities = 16/22 (72%), Positives = 20/22 (90%)
Frame = +3
Query: 900 IMIEGFKKGVIKFAIVTCSQLA 965
+MIEG+KKGVIKFAI+TC + A
Sbjct: 347 LMIEGYKKGVIKFAIITCRKPA 368
Score = 25.0 bits (53), Expect(3) = 2e-98
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +1
Query: 862 SGMKTIKGALAMPL 903
SG KTI+GA+ MPL
Sbjct: 334 SGWKTIRGAMVMPL 347
>ref|NP_176677.1| gamma-tocopherol methyltransferase; protein id: At1g64970.1,
supported by cDNA: 32143., supported by cDNA:
gi_15146233, supported by cDNA: gi_19699149, supported
by cDNA: gi_4106537 [Arabidopsis thaliana]
gi|25404508|pir||C96673 gamma-tocopherol
methyltransferase [imported] - Arabidopsis thaliana
gi|8099780|gb|AAD38271.2|AC006193_27 gamma-tocopherol
methyltransferase [Arabidopsis thaliana]
gi|15146234|gb|AAK83600.1| At1g64970/F13O11_27
[Arabidopsis thaliana] gi|19699150|gb|AAL90941.1|
At1g64970/F13O11_27 [Arabidopsis thaliana]
Length = 348
Score = 339 bits (869), Expect(3) = 7e-98
Identities = 165/232 (71%), Positives = 192/232 (82%), Gaps = 3/232 (1%)
Frame = +2
Query: 188 AVMEEKKLLQTGIAEFYDESSGLWEDMWGDHMHHGFYEQDVTVSVSD--HRVAQIRMIEE 361
A + L+ GIAEFY+E+SGLWE++WGDHMHHGFY+ D +V +SD H+ AQIRMIEE
Sbjct: 52 AAATSTEALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMIEE 111
Query: 362 SLRFAALS-EDPAKKPESIVDVGCGIGGSSRYLAKKFQAKSVGITLSPVQAQRANALAAS 538
SLRFA ++ E+ KK + +VDVGCGIGGSSRYLA KF A+ +GITLSPVQA+RAN LAA+
Sbjct: 112 SLRFAGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAA 171
Query: 539 QGLADKVSFQVADALEQPFPDGQFDLVWSMESGEHMPDKPKFVGELARVAAPGGTIIIVT 718
Q LA K SFQVADAL+QPF DG+FDLVWSMESGEHMPDK KFV EL RVAAPGG IIIVT
Sbjct: 172 QSLAHKASFQVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVT 231
Query: 719 WCHRDLGPAEESLQPWEQNLLKRICDAFYLPAWCSTADYVKLLESHSLQE*K 874
WCHR+L EE+LQPWEQN+L +IC FYLPAWCST DYV LL+SHSLQ+ K
Sbjct: 232 WCHRNLSAGEEALQPWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQDIK 283
Score = 36.2 bits (82), Expect(3) = 7e-98
Identities = 14/20 (70%), Positives = 18/20 (90%)
Frame = +3
Query: 900 IMIEGFKKGVIKFAIVTCSQ 959
+MIEG+KKGVIKF I+TC +
Sbjct: 327 LMIEGYKKGVIKFGIITCQK 346
Score = 27.7 bits (60), Expect(3) = 7e-98
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +1
Query: 862 SGMKTIKGALAMPL 903
SGMK+IKGAL MPL
Sbjct: 314 SGMKSIKGALTMPL 327
>sp|Q9ZSK1|GTOM_ARATH Tocopherol O-methyltransferase, chloroplast precursor
(Gamma-tocopherol methyltransferase)
gi|4106538|gb|AAD02882.1| gamma-tocopherol
methyltransferase [Arabidopsis thaliana]
Length = 348
Score = 338 bits (866), Expect(3) = 2e-97
Identities = 164/232 (70%), Positives = 192/232 (82%), Gaps = 3/232 (1%)
Frame = +2
Query: 188 AVMEEKKLLQTGIAEFYDESSGLWEDMWGDHMHHGFYEQDVTVSVSD--HRVAQIRMIEE 361
A + L+ GIAEFY+E+SGLWE++WGDHMHHGFY+ D +V +SD H+ AQIRMIEE
Sbjct: 52 AAATSTEALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMIEE 111
Query: 362 SLRFAALS-EDPAKKPESIVDVGCGIGGSSRYLAKKFQAKSVGITLSPVQAQRANALAAS 538
SLRFA ++ E+ KK + +VDVGCGIGGSSRYLA KF A+ +GITLSPVQA+RAN LAA+
Sbjct: 112 SLRFAGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAA 171
Query: 539 QGLADKVSFQVADALEQPFPDGQFDLVWSMESGEHMPDKPKFVGELARVAAPGGTIIIVT 718
Q L+ K SFQVADAL+QPF DG+FDLVWSMESGEHMPDK KFV EL RVAAPGG IIIVT
Sbjct: 172 QSLSHKASFQVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVT 231
Query: 719 WCHRDLGPAEESLQPWEQNLLKRICDAFYLPAWCSTADYVKLLESHSLQE*K 874
WCHR+L EE+LQPWEQN+L +IC FYLPAWCST DYV LL+SHSLQ+ K
Sbjct: 232 WCHRNLSAGEEALQPWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQDIK 283
Score = 36.2 bits (82), Expect(3) = 2e-97
Identities = 14/20 (70%), Positives = 18/20 (90%)
Frame = +3
Query: 900 IMIEGFKKGVIKFAIVTCSQ 959
+MIEG+KKGVIKF I+TC +
Sbjct: 327 LMIEGYKKGVIKFGIITCQK 346
Score = 27.7 bits (60), Expect(3) = 2e-97
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +1
Query: 862 SGMKTIKGALAMPL 903
SGMK+IKGAL MPL
Sbjct: 314 SGMKSIKGALTMPL 327
>gb|AAO13806.1| gamma-tocopherol methyltransferase [Brassica oleracea]
Length = 347
Score = 340 bits (871), Expect(3) = 2e-97
Identities = 179/289 (61%), Positives = 214/289 (73%), Gaps = 5/289 (1%)
Frame = +2
Query: 23 MMSIFPPPPSVASLFILSHCTHTIRVQST---TQFTGFSIRTRTRDCSRILLTEEREMAV 193
M + PP S+ SL H ++R S +Q ++ T T + +T A
Sbjct: 1 MKATLAPPSSLISL--PRHKVSSLRSPSLLLQSQRPSSALMTTTATRGSVAVTA----AA 54
Query: 194 MEEKKLLQTGIAEFYDESSGLWEDMWGDHMHHGFYEQDVTVSVSD--HRVAQIRMIEESL 367
+ L+ GIAEFY+E+SGLWE++WGDHMHHGFY+ D +V +SD HR AQIRMIEESL
Sbjct: 55 TSSAEALREGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHREAQIRMIEESL 114
Query: 368 RFAALSEDPAKKPESIVDVGCGIGGSSRYLAKKFQAKSVGITLSPVQAQRANALAASQGL 547
RFA ++E+ KK + +VDVGCGIGGSSRY+A KF A+ +GITLSPVQA+RAN LAA+Q L
Sbjct: 115 RFAGVTEEE-KKIKRVVDVGCGIGGSSRYIASKFGAECIGITLSPVQAKRANDLAAAQSL 173
Query: 548 ADKVSFQVADALEQPFPDGQFDLVWSMESGEHMPDKPKFVGELARVAAPGGTIIIVTWCH 727
+ KVSFQVADAL+QPF DG FDLVWSMESGEHMPDK KFV EL RV APGG IIIVTWCH
Sbjct: 174 SHKVSFQVADALDQPFEDGIFDLVWSMESGEHMPDKAKFVKELVRVTAPGGRIIIVTWCH 233
Query: 728 RDLGPAEESLQPWEQNLLKRICDAFYLPAWCSTADYVKLLESHSLQE*K 874
R+L EESLQPWEQNLL RIC FYLPAWCST+DYV+LL+S SLQ+ K
Sbjct: 234 RNLSQGEESLQPWEQNLLDRICKTFYLPAWCSTSDYVELLQSLSLQDIK 282
Score = 34.3 bits (77), Expect(3) = 2e-97
Identities = 13/20 (65%), Positives = 17/20 (85%)
Frame = +3
Query: 900 IMIEGFKKGVIKFAIVTCSQ 959
+MIEG+KKGVIKF I+ C +
Sbjct: 326 LMIEGYKKGVIKFGIIACQK 345
Score = 27.7 bits (60), Expect(3) = 2e-97
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +1
Query: 862 SGMKTIKGALAMPL 903
SGMK+IKGAL MPL
Sbjct: 313 SGMKSIKGALTMPL 326
>gb|AAM64696.1| gamma-tocopherol methyltransferase [Arabidopsis thaliana]
Length = 348
Score = 336 bits (862), Expect(3) = 4e-97
Identities = 163/232 (70%), Positives = 192/232 (82%), Gaps = 3/232 (1%)
Frame = +2
Query: 188 AVMEEKKLLQTGIAEFYDESSGLWEDMWGDHMHHGFYEQDVTVSVSD--HRVAQIRMIEE 361
A + L+ GIAEFY+E+SGLWE++WGDHMHHGFY+ D +V +SD H+ AQIRMIEE
Sbjct: 52 AAATSTEALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMIEE 111
Query: 362 SLRFAALS-EDPAKKPESIVDVGCGIGGSSRYLAKKFQAKSVGITLSPVQAQRANALAAS 538
SLRFA ++ E+ KK + +VDVGCGIGGSSRYLA KF A+ +GITLSPVQA+RAN LAA+
Sbjct: 112 SLRFAGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAA 171
Query: 539 QGLADKVSFQVADALEQPFPDGQFDLVWSMESGEHMPDKPKFVGELARVAAPGGTIIIVT 718
Q L+ K SFQVADAL++PF DG+FDLVWSMESGEHMPDK KFV EL RVAAPGG IIIVT
Sbjct: 172 QSLSHKASFQVADALDKPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVT 231
Query: 719 WCHRDLGPAEESLQPWEQNLLKRICDAFYLPAWCSTADYVKLLESHSLQE*K 874
WCHR+L EE+LQPWEQN+L +IC FYLPAWCST DYV LL+SHSLQ+ K
Sbjct: 232 WCHRNLSAGEEALQPWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQDIK 283
Score = 36.2 bits (82), Expect(3) = 4e-97
Identities = 14/20 (70%), Positives = 18/20 (90%)
Frame = +3
Query: 900 IMIEGFKKGVIKFAIVTCSQ 959
+MIEG+KKGVIKF I+TC +
Sbjct: 327 LMIEGYKKGVIKFGIITCQK 346
Score = 27.7 bits (60), Expect(3) = 4e-97
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +1
Query: 862 SGMKTIKGALAMPL 903
SGMK+IKGAL MPL
Sbjct: 314 SGMKSIKGALTMPL 327
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,093,534,277
Number of Sequences: 1393205
Number of extensions: 24406677
Number of successful extensions: 73404
Number of sequences better than 10.0: 1090
Number of HSP's better than 10.0 without gapping: 68462
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 72975
length of database: 448,689,247
effective HSP length: 126
effective length of database: 273,145,417
effective search space used: 78119589262
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
