Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000637A_C01 KMC000637A_c01
(572 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q39891|LEU1_SOYBN Probable 2-isopropylmalate synthase (Alpha-... 198 2e-63
emb|CAC80103.1| hypothetical protein [Arabidopsis thaliana] 160 9e-52
gb|AAG52883.1|AF327648_1 2-isopropylmalate synthase [Arabidopsis... 160 2e-51
ref|NP_197693.1| 2-isopropylmalate synthase-like protein; protei... 159 3e-51
emb|CAD18966.1| 2-isopropylmalate synthase [Arabidopsis thaliana] 158 5e-51
>sp|Q39891|LEU1_SOYBN Probable 2-isopropylmalate synthase (Alpha-isopropylmalate
synthase) (Alpha-IPM synthetase) (Late nodulin 56)
(N-56) gi|7447056|pir||T08590 2-isopropylmalate synthase
homolog - soybean gi|532290|dbj|BAA07212.1| late nodulin
[Glycine max]
Length = 565
Score = 198 bits (503), Expect(2) = 2e-63
Identities = 98/128 (76%), Positives = 111/128 (86%)
Frame = +2
Query: 173 QLETARQLAKLGVDIIEAGFPCASKQDFMAVKMIAEEVGNCVDGNGYVPVITGVSRCNEK 352
+L+ ARQL KLGVDII+ GFP AS DFMAVKMIA+EVGN VD +GYVPVI G RC EK
Sbjct: 56 KLDIARQLVKLGVDIIQPGFPSASNSDFMAVKMIAQEVGNAVDDDGYVPVIAGFCRCVEK 115
Query: 353 DIATAWEALKHAKRPRLRTFIATSPIHMEYKLRKSKDQVLETARNMVKFARSLGCTDIQF 532
DI+TAWEA+K+AKRPRL T IATSPIHME+KLRKSKDQV++ AR+MVKFARSLGC DIQF
Sbjct: 116 DISTAWEAVKYAKRPRLCTSIATSPIHMEHKLRKSKDQVIQIARDMVKFARSLGCNDIQF 175
Query: 533 GAEDAARN 556
GAEDA R+
Sbjct: 176 GAEDATRS 183
Score = 66.2 bits (160), Expect(2) = 2e-63
Identities = 29/34 (85%), Positives = 32/34 (93%)
Frame = +1
Query: 61 RPEYIPNHIPNPTYVRILDTTLRDGEQSPGAAMT 162
RP+YIPNHIP+ +YVRILDTTLRDGEQSPGA MT
Sbjct: 19 RPQYIPNHIPDSSYVRILDTTLRDGEQSPGATMT 52
>emb|CAC80103.1| hypothetical protein [Arabidopsis thaliana]
Length = 503
Score = 160 bits (404), Expect(2) = 9e-52
Identities = 81/136 (59%), Positives = 105/136 (76%), Gaps = 2/136 (1%)
Frame = +2
Query: 170 RQLETARQLAKLGVDIIEAGFPCASKQDFMAVKMIAEEVGNCVDGN-GYVPVITGVSRCN 346
++LE ARQLAKL VDI+E GFP +S+++F A+K IA+ VGN VD GYVPVI G++RC
Sbjct: 107 QKLEIARQLAKLRVDIMEVGFPVSSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCK 166
Query: 347 EKDIATAWEALKHAKRPRLRTFIATSPIHMEYKLRKSKDQVLETARNMVKFARSLGCTDI 526
++DI WEALK+AKRPR+ F +TS IHM+YKL+K+K++V+E A N VK+A+SLG DI
Sbjct: 167 KRDIEATWEALKYAKRPRVMLFTSTSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDI 226
Query: 527 QFGAEDAAR-NFDFLC 571
QFG ED R DFLC
Sbjct: 227 QFGCEDGGRTEKDFLC 242
Score = 65.5 bits (158), Expect(2) = 9e-52
Identities = 28/38 (73%), Positives = 33/38 (86%)
Frame = +1
Query: 49 IIRNRPEYIPNHIPNPTYVRILDTTLRDGEQSPGAAMT 162
I+ RPEYIPN +P+ YVR+LDTTLRDGEQSPGAA+T
Sbjct: 67 IVERRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALT 104
>gb|AAG52883.1|AF327648_1 2-isopropylmalate synthase [Arabidopsis thaliana]
Length = 503
Score = 160 bits (404), Expect(2) = 2e-51
Identities = 80/136 (58%), Positives = 106/136 (77%), Gaps = 2/136 (1%)
Frame = +2
Query: 170 RQLETARQLAKLGVDIIEAGFPCASKQDFMAVKMIAEEVGNCVDGN-GYVPVITGVSRCN 346
++LE ARQLAKL VDI+E GFP +S+++F A+K IA+ VGN VD GYVPVI+G++RC
Sbjct: 107 QKLEIARQLAKLRVDIMEVGFPVSSEEEFEAIKTIAKTVGNEVDEETGYVPVISGIARCK 166
Query: 347 EKDIATAWEALKHAKRPRLRTFIATSPIHMEYKLRKSKDQVLETARNMVKFARSLGCTDI 526
++DI WEALK+AKRPR+ F +TS IHM+YKL+K+K++V+E A N VK+A+SLG DI
Sbjct: 167 KRDIEATWEALKYAKRPRVMLFTSTSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDI 226
Query: 527 QFGAEDAAR-NFDFLC 571
QFG ED R DF+C
Sbjct: 227 QFGCEDGGRTEKDFIC 242
Score = 64.7 bits (156), Expect(2) = 2e-51
Identities = 28/38 (73%), Positives = 33/38 (86%)
Frame = +1
Query: 49 IIRNRPEYIPNHIPNPTYVRILDTTLRDGEQSPGAAMT 162
I+ RPEYIPN +P+ YVR+LDTTLRDGEQSPGAA+T
Sbjct: 67 IMERRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALT 104
>ref|NP_197693.1| 2-isopropylmalate synthase-like protein; protein id: At5g23020.1,
supported by cDNA: gi_12330688, supported by cDNA:
gi_20466166 [Arabidopsis thaliana]
gi|9759360|dbj|BAB09819.1| 2-isopropylmalate
synthase-like protein [Arabidopsis thaliana]
gi|20466167|gb|AAM20401.1| 2-isopropylmalate
synthase-like protein [Arabidopsis thaliana]
Length = 503
Score = 159 bits (402), Expect(2) = 3e-51
Identities = 80/136 (58%), Positives = 105/136 (76%), Gaps = 2/136 (1%)
Frame = +2
Query: 170 RQLETARQLAKLGVDIIEAGFPCASKQDFMAVKMIAEEVGNCVDGN-GYVPVITGVSRCN 346
++LE ARQLAKL VDI+E GFP +S+++F A+K IA+ VGN VD GYVPVI G++RC
Sbjct: 107 QKLEIARQLAKLRVDIMEVGFPVSSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCK 166
Query: 347 EKDIATAWEALKHAKRPRLRTFIATSPIHMEYKLRKSKDQVLETARNMVKFARSLGCTDI 526
++DI WEALK+AKRPR+ F +TS IHM+YKL+K+K++V+E A N VK+A+SLG DI
Sbjct: 167 KRDIEATWEALKYAKRPRVMLFTSTSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDI 226
Query: 527 QFGAEDAAR-NFDFLC 571
QFG ED R DF+C
Sbjct: 227 QFGCEDGGRTEKDFIC 242
Score = 64.7 bits (156), Expect(2) = 3e-51
Identities = 28/38 (73%), Positives = 33/38 (86%)
Frame = +1
Query: 49 IIRNRPEYIPNHIPNPTYVRILDTTLRDGEQSPGAAMT 162
I+ RPEYIPN +P+ YVR+LDTTLRDGEQSPGAA+T
Sbjct: 67 IMERRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALT 104
>emb|CAD18966.1| 2-isopropylmalate synthase [Arabidopsis thaliana]
Length = 503
Score = 158 bits (400), Expect(2) = 5e-51
Identities = 80/136 (58%), Positives = 104/136 (75%), Gaps = 2/136 (1%)
Frame = +2
Query: 170 RQLETARQLAKLGVDIIEAGFPCASKQDFMAVKMIAEEVGNCVDGN-GYVPVITGVSRCN 346
++LE ARQLAKL VDI+E GFP +S+++F A+K IA+ VGN VD GY PVI G++RC
Sbjct: 107 QKLEIARQLAKLRVDIMEVGFPVSSEEEFEAIKTIAKTVGNEVDEETGYAPVICGIARCK 166
Query: 347 EKDIATAWEALKHAKRPRLRTFIATSPIHMEYKLRKSKDQVLETARNMVKFARSLGCTDI 526
++DI WEALK+AKRPR+ F +TS IHM+YKL+K+K++V+E A N VK+A+SLG DI
Sbjct: 167 KRDIEATWEALKYAKRPRVMLFTSTSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDI 226
Query: 527 QFGAEDAAR-NFDFLC 571
QFG ED R DFLC
Sbjct: 227 QFGCEDGGRTEKDFLC 242
Score = 64.7 bits (156), Expect(2) = 5e-51
Identities = 28/38 (73%), Positives = 33/38 (86%)
Frame = +1
Query: 49 IIRNRPEYIPNHIPNPTYVRILDTTLRDGEQSPGAAMT 162
I+ RPEYIPN +P+ YVR+LDTTLRDGEQSPGAA+T
Sbjct: 67 IMERRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALT 104
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 551,640,732
Number of Sequences: 1393205
Number of extensions: 12893027
Number of successful extensions: 41664
Number of sequences better than 10.0: 241
Number of HSP's better than 10.0 without gapping: 39391
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 41485
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 21243732558
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
