>KMC000635A_C01 KMC000635A_c01
gtttgAAGAAAATGACTCATGGCCTGGAATGCATGTGTGACAATGTAGGAGTAGCTTAAG
TGGTAGCATAACCGGCTGCGCTTTATAATTATTATATTTTCTTTTGATAGGCTTAGAATT
ATTATATTATAAATATGTAGATAGATTGTTTGCAGCTGGTTAACCCCTTAGAGGCAGCTA
CAAAAGAATACAAAGGTCTCTAGCAATACCAAGACTAGTAGTATACACTAATGCTTCCTC
ATTCCTACCGTATCCTCCTGCAAAATAACTGGGCTCTGCTACATCTCAATTGTTCAAGGT
TGGTTGAATTTGGGATTTAAGATCGGAATATATCTCTAGGACAAAGAATTTGGGGTCCAC
AAGTGTCCCTAAGATAATTCAGCGACAGAACCATTCCCATTCTCTAACTCTGAGAGCTCA
CTCTCAAAAAAGGCAGAATGAAGAGCCCTGACACATTGCTCTGCTTCATTATCATTTACA
ACCAATGAGATGTTCACCTTAGATGCACCCTGAGAGATCATTTGAACAGTGACACCGAGG
GATCGAAGAACACGAAATGCCTTCTCCAATATTAGTGATGACCTCTGAACATTTCCAATG
AGAGAGATGATGGA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000635A_C01 KMC000635A_c01
         (614 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAD41796.1| precursor monofunctional aspartokinase [Glycine max]   134  7e-31
gb|AAB63104.1| lysine-sensitive aspartate kinase [Arabidopsis th...   109  3e-23
ref|NP_196910.1| lysine-sensitive aspartate kinase (gb|AAB63104....   109  3e-23
emb|CAC06395.1| aspartate kinase [Arabidopsis thaliana]               109  3e-23
ref|NP_186851.1| putative aspartate kinase; protein id: At3g0202...   108  4e-23

>gb|AAD41796.1| precursor monofunctional aspartokinase [Glycine max]
          Length = 564

 Score =  134 bits (338), Expect = 7e-31
 Identities = 75/85 (88%), Positives = 78/85 (91%), Gaps = 4/85 (4%)
 Frame = -1

Query: 614 SIISLIGNVQRSSLILEKAFRVLRSLGVTVQMISQGASKVNISLVVNDNEAEQCVRALHS 435
           SIISLIGNVQRSSLILE+  RVLR+LGVTVQMISQGASKVNISLVVND+EAEQCVRALHS
Sbjct: 480 SIISLIGNVQRSSLILERLSRVLRTLGVTVQMISQGASKVNISLVVNDSEAEQCVRALHS 539

Query: 434 AFFESELSELE----NGNGSVAELS 372
           AFFESELSELE    NGNGSV ELS
Sbjct: 540 AFFESELSELEMDYKNGNGSVDELS 564

>gb|AAB63104.1| lysine-sensitive aspartate kinase [Arabidopsis thaliana]
          Length = 544

 Score =  109 bits (272), Expect = 3e-23
 Identities = 56/66 (84%), Positives = 61/66 (91%)
 Frame = -1

Query: 614 SIISLIGNVQRSSLILEKAFRVLRSLGVTVQMISQGASKVNISLVVNDNEAEQCVRALHS 435
           SIISLIGNVQ+SSLILEK F+V RS GV VQMISQGASKVNISL+VND EAEQCVRALHS
Sbjct: 478 SIISLIGNVQKSSLILEKVFQVFRSNGVNVQMISQGASKVNISLIVNDEEAEQCVRALHS 537

Query: 434 AFFESE 417
           AFFE++
Sbjct: 538 AFFETD 543

>ref|NP_196910.1| lysine-sensitive aspartate kinase (gb|AAB63104.1); protein id:
           At5g14060.1, supported by cDNA: gi_2257742 [Arabidopsis
           thaliana] gi|9757787|dbj|BAB08285.1| lysine-sensitive
           aspartate kinase [Arabidopsis thaliana]
          Length = 544

 Score =  109 bits (272), Expect = 3e-23
 Identities = 56/66 (84%), Positives = 61/66 (91%)
 Frame = -1

Query: 614 SIISLIGNVQRSSLILEKAFRVLRSLGVTVQMISQGASKVNISLVVNDNEAEQCVRALHS 435
           SIISLIGNVQ+SSLILEK F+V RS GV VQMISQGASKVNISL+VND EAEQCVRALHS
Sbjct: 478 SIISLIGNVQKSSLILEKVFQVFRSNGVNVQMISQGASKVNISLIVNDEEAEQCVRALHS 537

Query: 434 AFFESE 417
           AFFE++
Sbjct: 538 AFFETD 543

>emb|CAC06395.1| aspartate kinase [Arabidopsis thaliana]
          Length = 544

 Score =  109 bits (272), Expect = 3e-23
 Identities = 56/66 (84%), Positives = 61/66 (91%)
 Frame = -1

Query: 614 SIISLIGNVQRSSLILEKAFRVLRSLGVTVQMISQGASKVNISLVVNDNEAEQCVRALHS 435
           SIISLIGNVQ+SSLILEK F+V RS GV VQMISQGASKVNISL+VND EAEQCVRALHS
Sbjct: 478 SIISLIGNVQKSSLILEKVFQVFRSNGVNVQMISQGASKVNISLIVNDEEAEQCVRALHS 537

Query: 434 AFFESE 417
           AFFE++
Sbjct: 538 AFFETD 543

>ref|NP_186851.1| putative aspartate kinase; protein id: At3g02020.1, supported by
           cDNA: 6203. [Arabidopsis thaliana]
           gi|6091740|gb|AAF03452.1|AC010797_28 putative aspartate
           kinase [Arabidopsis thaliana]
           gi|6513929|gb|AAF14833.1|AC011664_15 putative aspartate
           kinase [Arabidopsis thaliana] gi|21593967|gb|AAM65905.1|
           putative aspartate kinase [Arabidopsis thaliana]
          Length = 559

 Score =  108 bits (271), Expect = 4e-23
 Identities = 55/69 (79%), Positives = 62/69 (89%)
 Frame = -1

Query: 614 SIISLIGNVQRSSLILEKAFRVLRSLGVTVQMISQGASKVNISLVVNDNEAEQCVRALHS 435
           SIISLIGNVQRSS ILEK FRVLR+ G+ VQMISQGASKVNISL+VND+EAE CV+ALHS
Sbjct: 477 SIISLIGNVQRSSFILEKGFRVLRTNGINVQMISQGASKVNISLIVNDDEAEHCVKALHS 536

Query: 434 AFFESELSE 408
           AFFE++  E
Sbjct: 537 AFFETDTCE 545

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 484,930,127
Number of Sequences: 1393205
Number of extensions: 9665312
Number of successful extensions: 18920
Number of sequences better than 10.0: 137
Number of HSP's better than 10.0 without gapping: 18469
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18910
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 24854530794
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf069g11 BP072507 1 505 2 GENLf027g10 BP063793 4 505 3 GENf036g03 BP059904 69 380 4 GENf035h01 BP059861 80 441 5 MR069e07_f BP081310 91 491 6 GENf042g08 BP060146 111 464 7 GENf038f10 BP059999 111 635 8 GNf088h02 BP073896 111 611 9 GENLf033f06 BP064086 111 639 10 GENf060d02 BP060895 122 499 11 MWM128g02_f AV766784 123 558 12 MWM061c01_f AV765663 124 443

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