>KMC000566A_C03 KMC000566A_c03
gggacAAAAGGAGAAGAGGAGCTTTCATGGCTCCAATATATAATTAGCAATACAATTGGT
CTGGACAGGGAGGGGAAAAAAACTCTTCATGGGGGGTCCCCTCAAATATACAATTGGTTA
CAAAGGCTACCTAATAATGTAACAACCAAAATTAAGGGGCCTAAGTTAGAGCTCCTAAAG
AAGGGGAAGAAAGGGTTAACTTACAAATCAGGAAAGGAAAGGCTAGTATACAAAAAGAAT
GACACTAAACCACAGTTTCTAATCTACAAATCTGGTTGGTATTTTGGGAGCATATACAAA
AGGGTGACGGACTTAATAATTTCCCACCCGTGATGTAAACAGCCTATTATGGTTAATGAA
GGCAATTAACTTACAATGAATGGGGAGGTGTTGATTGGAAAGAGGAATCCTCTTGGAACA
CGTACAGACGGGCTCGATAAATCTACTGCGGTTTCAAGGTTGAGCAAAGGTTTCAGTCTT
CTATGCACCTCGTCACTTAGAAAATTAATCTCTTCATGAATCATGAGTAACGCAGACTT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000566A_C03 KMC000566A_c03
         (539 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_560885.1| hypothetical protein [Pyrobaculum aerophilum] g...    34  1.3
gb|EAA28050.1| hypothetical protein [Neurospora crassa]                33  2.9
gb|EAA18789.1| DNA-dependent RNA polymerase-related [Plasmodium ...    32  4.9
ref|NP_636254.1| conserved hypothetical protein [Xanthomonas cam...    32  6.4
sp|Q25490|APLP_MANSE Apolipophorins precursor [Contains: Apolipo...    32  6.4

>ref|NP_560885.1| hypothetical protein [Pyrobaculum aerophilum]
           gi|18161812|gb|AAL65067.1| hypothetical protein
           [Pyrobaculum aerophilum]
          Length = 280

 Score = 33.9 bits (76), Expect = 1.3
 Identities = 22/55 (40%), Positives = 31/55 (56%), Gaps = 4/55 (7%)
 Frame = -1

Query: 485 HRRLKPLLNLET---AVDLSSPSVRVPRGFLFPIN-TSPFIVS*LPSLTIIGCLH 333
           H   +PL  LET   A+ +S+P V++PR ++FP    +PF     PSL  IG  H
Sbjct: 86  HITFQPLEELETYASALAMSNPPVKLPRNYVFPARWRAPF----KPSLERIGVFH 136

>gb|EAA28050.1| hypothetical protein [Neurospora crassa]
          Length = 960

 Score = 32.7 bits (73), Expect = 2.9
 Identities = 18/44 (40%), Positives = 25/44 (55%)
 Frame = +1

Query: 118 LQRLPNNVTTKIKGPKLELLKKGKKGLTYKSGKERLVYKKNDTK 249
           LQ + +NV+ + K PK    KKGKKG   KS K +    K D++
Sbjct: 138 LQEIHSNVSRQRKQPKPGKGKKGKKGKAKKSSKPKTAPAKEDSE 181

>gb|EAA18789.1| DNA-dependent RNA polymerase-related [Plasmodium yoelii yoelii]
          Length = 674

 Score = 32.0 bits (71), Expect = 4.9
 Identities = 26/97 (26%), Positives = 46/97 (46%), Gaps = 16/97 (16%)
 Frame = +1

Query: 7   KGEEELSWLQYIISNT---IGLDREGKKTLH-----GGSPQIYNWLQRLPNN-------- 138
           K E  +++L YI SN+   + L ++ +  +H       S + YN       N        
Sbjct: 16  KKERAINYLLYIKSNSFSNVNLTKKKEANIHIIYNNEDSVKCYNNKNECKKNDELDKNNE 75

Query: 139 VTTKIKGPKLELLKKGKKGLTYKSGKERLVYKKNDTK 249
           + +  K  KL+  KK KK   Y++ +++L+Y +ND K
Sbjct: 76  IISFYKSEKLKNEKKKKKCNNYENEQDKLIYVENDEK 112

>ref|NP_636254.1| conserved hypothetical protein [Xanthomonas campestris pv.
           campestris str. ATCC 33913] gi|21111889|gb|AAM40178.1|
           conserved hypothetical protein [Xanthomonas campestris
           pv. campestris str. ATCC 33913]
          Length = 407

 Score = 31.6 bits (70), Expect = 6.4
 Identities = 16/42 (38%), Positives = 24/42 (57%)
 Frame = +1

Query: 385 EVLIGKRNPLGTRTDGLDKSTAVSRLSKGFSLLCTSSLRKLI 510
           EVL+   +PLG +TD +D   A+  L +GF  L    L +L+
Sbjct: 335 EVLLASCSPLGPQTDDVDYIEAMRPLHRGFMQLFAQRLSQLV 376

>sp|Q25490|APLP_MANSE Apolipophorins precursor [Contains: Apolipophorin-I (APOLP-1);
            Apolipophorin-II (APOLP-2)] gi|7511788|pir||T18358
            apolipophorin precursor protein - tobacco hornworm
            gi|1399218|gb|AAB53254.1| apolipophorin precursor protein
          Length = 3305

 Score = 31.6 bits (70), Expect = 6.4
 Identities = 17/60 (28%), Positives = 30/60 (49%)
 Frame = +1

Query: 55   IGLDREGKKTLHGGSPQIYNWLQRLPNNVTTKIKGPKLELLKKGKKGLTYKSGKERLVYK 234
            +GL+  GK  ++G     + W  + PN +   +KG   ++   GK G + +  + R VYK
Sbjct: 1046 LGLEAVGK-VIYG-----HIWAHKKPNELVASVKGKLDDIEYSGKLGFSVQGNEHRAVYK 1099

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 471,039,023
Number of Sequences: 1393205
Number of extensions: 10390724
Number of successful extensions: 22634
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 22166
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22629
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 18462123008
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENf025b12 BP059381 1 321 2 GENLf023h08 BP063575 7 547 3 GENLf023h12 BP063577 7 508 4 MFL017h01_f BP033827 7 589 5 MPDL013d07_f AV777179 7 588 6 GENLf041c07 BP064498 8 579 7 MFL012f11_f BP033769 8 601 8 GENLf032a11 BP064006 8 589 9 GENLf082f08 BP066824 9 577 10 MRL021e09_f BP084794 12 436 11 SPDL096g08_f BP058056 13 568 12 GENLf035c12 BP064175 13 584 13 GENLf025f09 BP063663 18 446

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