>KMC000468A_C01 KMC000468A_c01
gttttGGTAGAACTCCTCAGATGAATTGAATAAAAAAAATATGCCACAATGAAGCTCCAC
TTCAAAGCAGATCTAACAGGAAATAATGAAATCATAAAGCATAGTAGTAGAAATAATTTC
TATCCCTTTTACTATGCTCCATACATGTATAATAATTAACTGCCAATAATTTGATTTGAA
AACAGAATGATGAAAAATTTAGTTGGAAAAATAACTCAACATTCCTATTTTATTTACATG
TTGTAACACCTAAAACGAGCTCCGCATGTACCTTTACACCTTGAGAATGTGGCGAACCGA
TTCAACTGCACTTCTGCCACAGCCTTTGATGAACGCCCCAAATTAGGCCATTATTTGAGA
GGGAAGGAGGATATTACATGCAACCAAAAATTAGGCATCAGAGAGTAAACCCTGATTCAT
TATATTAACATACAGGGTAAGGTTGGTTAGTTTCAGAAACTCATGATAAATCAAAGGATC
CCTGGCCTTCATTGATTGCATATAGTAGCTTCTTGTACATTATTTCCTTGGTAGAATAAG
GAGGAAGCTTCAGGTAATTAGCACATG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000468A_C01 KMC000468A_c01
         (567 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_195572.1| putative protein; protein id: At4g38600.1 [Arab...    74  1e-12
pir||T06977 hypothetical protein Tap - wheat (fragment) gi|16676...    65  6e-10
ref|NP_195908.1| putative protein; protein id: At5g02880.1 [Arab...    59  4e-08
ref|NP_004229.1| thyroid hormone receptor interactor 12; thyroid...    47  2e-04
ref|NP_012915.1| Ubiquitin Fusion Degradation; Ufd4p [Saccharomy...    46  4e-04

>ref|NP_195572.1| putative protein; protein id: At4g38600.1 [Arabidopsis thaliana]
           gi|7485881|pir||T05688 hypothetical protein F20M13.160 -
           Arabidopsis thaliana gi|4467147|emb|CAB37516.1| putative
           protein [Arabidopsis thaliana]
           gi|7270843|emb|CAB80524.1| putative protein [Arabidopsis
           thaliana]
          Length = 757

 Score = 73.9 bits (180), Expect = 1e-12
 Identities = 34/34 (100%), Positives = 34/34 (100%)
 Frame = -3

Query: 565 CANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 464
           CANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS
Sbjct: 724 CANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 757

>pir||T06977 hypothetical protein Tap - wheat (fragment)
           gi|1667607|gb|AAB18822.1| Tap [Triticum aestivum]
          Length = 94

 Score = 65.1 bits (157), Expect = 6e-10
 Identities = 30/34 (88%), Positives = 32/34 (93%)
 Frame = -3

Query: 565 CANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 464
           CANYLKLPPYSTKE+M KKLLYAI EG+GSFDLS
Sbjct: 61  CANYLKLPPYSTKEVMRKKLLYAILEGRGSFDLS 94

>ref|NP_195908.1| putative protein; protein id: At5g02880.1 [Arabidopsis thaliana]
            gi|11357909|pir||T48309 hypothetical protein F9G14.190 -
            Arabidopsis thaliana gi|7413563|emb|CAB86042.1| putative
            protein [Arabidopsis thaliana]
          Length = 1502

 Score = 58.9 bits (141), Expect = 4e-08
 Identities = 27/34 (79%), Positives = 30/34 (87%)
 Frame = -3

Query: 565  CANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 464
            CANYLKLPPYS+KE M +KL+YAI EGQGSF LS
Sbjct: 1469 CANYLKLPPYSSKEKMKEKLIYAITEGQGSFHLS 1502

>ref|NP_004229.1| thyroid hormone receptor interactor 12; thyroid receptor interacting
            protein 12 [Homo sapiens] gi|2499839|sp|Q14669|TRIB_HUMAN
            Thyroid receptor interacting protein 12 (TRIP12)
            gi|460711|dbj|BAA05837.1| KIAA0045 [Homo sapiens]
          Length = 1992

 Score = 46.6 bits (109), Expect = 2e-04
 Identities = 23/34 (67%), Positives = 25/34 (72%)
 Frame = -3

Query: 565  CANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 464
            C NYLKLP YS+ EIM +KLL A  EGQ SF LS
Sbjct: 1959 CVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS 1992

>ref|NP_012915.1| Ubiquitin Fusion Degradation; Ufd4p [Saccharomyces cerevisiae]
            gi|465668|sp|P33202|UFD4_YEAST Ubiquitin fusion
            degradation protein 4 (UB fusion protein 4)
            gi|320759|pir||S30015 hypothetical protein YKL010c -
            yeast (Saccharomyces cerevisiae) gi|263499|gb|AAB24903.1|
            orf YKL162 [Saccharomyces cerevisiae]
            gi|485993|emb|CAA81845.1| ORF YKL010c [Saccharomyces
            cerevisiae]
          Length = 1483

 Score = 45.8 bits (107), Expect = 4e-04
 Identities = 21/34 (61%), Positives = 26/34 (75%)
 Frame = -3

Query: 565  CANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS 464
            CANYLKLP Y++K+IM  +L  AI EG G+F LS
Sbjct: 1450 CANYLKLPKYTSKDIMRSRLCQAIEEGAGAFLLS 1483

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 455,740,928
Number of Sequences: 1393205
Number of extensions: 9314044
Number of successful extensions: 17445
Number of sequences better than 10.0: 173
Number of HSP's better than 10.0 without gapping: 16948
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17439
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20669577624
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPDL075e04_f AV780368 1 518 2 MF009f04_f BP028702 5 574 3 MWM167e10_f AV767318 6 570 4 SPDL026a11_f BP053585 13 532 5 GNLf005a04 BP075053 76 465 6 MPDL064a05_f AV779733 82 494 7 MPDL013h12_f AV777207 86 434 8 SPDL052d11_f BP055286 94 489 9 GENLf018b03 BP063308 109 486

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