KMC000462A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC000462A_C01 KMC000462A_c01
tggtacccaggatGCTTAAAAAAAAAGTGGTCCTTGGGTTTTATTTTTCTATCTTTACAT
AATCGATGGCAGACAACTGAGTAATAAAGGCACCATGTTACAGGTTATTTTATTCCTCAA
CTACAATTATATCTAGTTTCAATTATACAAACATAGCTCCACATGATTTACGAAAGGCAG
TTTTAGAAGTTAGAACTGCCAAAATTCCCGCATCCGTGTGTGTTGTCTCTGTTGAAGCAC
TGGAAAAAGAAACTCAAACTAGAGCTCTGGCCAATCAAACAGCCAATAAGCATTGAAAAA
ATATAGGAGGGGCAGAGTAGGAGAGATCAGGTCTTGAACTTTTGGGTGATTTTCTCAACT
CCTTCCCTGCAATCACCAGCAACTCGCGTTTGCACCTTCTTTGATAGTAAATCCAAATTC
AAGGGCACTCTGCTGCAACTCAGTGGCTGACTTACCTGGCTTTGAGATGACAGCTTCTCC
TAATTCTTCAGTCTGTTTTCCTGTTTGGGATGCCCATTCCCTCATTTTCGATATGTATAG
GGAAATGATGTGGA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000462A_C01 KMC000462A_c01
         (554 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T28634 variant-specific surface protein 7 - malaria parasit...    32  6.8
ref|NP_616994.1| conserved hypothetical protein [Methanosarcina ...    31  8.9
gb|EAA21723.1| asparagine-rich protein [Plasmodium yoelii yoelii]      31  8.9

>pir||T28634 variant-specific surface protein 7 - malaria parasite  (Plasmodium
            falciparum) gi|886380|gb|AAA75399.1| variant-specific
            surface protein
          Length = 2182

 Score = 31.6 bits (70), Expect = 6.8
 Identities = 13/39 (33%), Positives = 23/39 (58%)
 Frame = -3

Query: 540  LYISKMREWASQTGKQTEELGEAVISKPGKSATELQQSA 424
            LY+  + +WAS+ G +T E+     S P +S +E  ++A
Sbjct: 912  LYVGGLSQWASRGGDETTEVSSEATSAPSQSESEKLRTA 950

>ref|NP_616994.1| conserved hypothetical protein [Methanosarcina acetivorans str.
           C2A] gi|19915998|gb|AAM05474.1| conserved hypothetical
           protein [Methanosarcina acetivorans str. C2A]
          Length = 498

 Score = 31.2 bits (69), Expect = 8.9
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = +2

Query: 398 SLIVNPNSRALCCNSVADLPGFEMTASPNSSVCFPVWDAHS 520
           +++V  +SR +CC     +PG E     N S C+P W  +S
Sbjct: 304 AVVVETSSRKVCCRY--PMPGVEALWQTNHSNCYPGWMGYS 342

>gb|EAA21723.1| asparagine-rich protein [Plasmodium yoelii yoelii]
          Length = 881

 Score = 31.2 bits (69), Expect = 8.9
 Identities = 15/51 (29%), Positives = 28/51 (54%)
 Frame = +2

Query: 368 CNHQQLAFAPSLIVNPNSRALCCNSVADLPGFEMTASPNSSVCFPVWDAHS 520
           CNH+ + ++P      NS  L   S A+L   +M    N+++C P++D ++
Sbjct: 37  CNHRDILYSPENSEGKNSSNLE-ESEAELLPLKMEMESNNNICNPIYDENN 86

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 476,863,496
Number of Sequences: 1393205
Number of extensions: 9711409
Number of successful extensions: 25720
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 25302
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25720
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19521267756
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 SPDL100h08_f BP058326 1 519
2 GENLf082h03 BP066838 14 544
3 GENLf063g03 BP065748 16 490
4 GNLf001h12 BP074878 17 532
5 SPDL085c01_f BP057315 18 402
6 GENLf083c07 BP066864 18 500
7 GENLf086b05 BP067017 18 435
8 GENLf050d07 BP065018 32 434
9 GENLf017f12 BP063284 62 562
10 GNLf002g03 BP074921 101 420




Lotus japonicus
Kazusa DNA Research Institute