>KMC000458A_C01 KMC000458A_c01
attgcaagtGGAAAATAACTTTATATATTTACACTACTACTAAGTAGTCAAAAAATCTGG
ACATACACTACATTCGAACCTAAAAAGGAGGAAAAGTCAAGCCTCACAATCCAGACAAGA
CTTTTCTTTCTTTTTTCTTCTTCCTTAAAAAGAATTATGAAACAGAAGACATATACTCCA
ACAACAACAATAGCTAATATACAAACAATACACATTATACACACATTTCTCAAAACACTA
GCAAATCATTCTTGACATCATATCCCATCTTCTCCAAATTCGGATTTACGGCAAACTGAA
TCATCGGTGGTGGCCCACAAGCCAATGCCAATGCATCATCCCCAGCCTCCGGAAGATGCT
CCCTCATAACACCCTCCGTGACGAATCCCACACTGTACCCCCACCCTTCCTTGGCGGTTT
CCACCACATACCACACCTTGAACCGGTCTTCGTACTTCTTCGCCCATGTGTCGAGCTCCT
CCCTTAAAAGAATATCATCCTCGGTCCTGTTTGCTTAGACCACATACATCTTAGT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000458A_C01 KMC000458A_c01
         (535 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|P39869|NIA_LOTJA Nitrate reductase [NADH] (NR) gi|632154|pir|...   209  1e-53
sp|P39866|NIA2_PHAVU Nitrate reductase [NADH] 2 (NR-2) gi|743265...   182  2e-45
sp|P43101|NIA_CICIN Nitrate reductase [NADH] (NR) gi|1086084|pir...   168  3e-41
gb|AAG30576.1|AF314093_1 nitrate reductase [Ricinus communis]         168  4e-41
prf||1713435B nitrate reductase                                       160  7e-39

>sp|P39869|NIA_LOTJA Nitrate reductase [NADH] (NR) gi|632154|pir||S47029 nitrate reductase
            (NADH) (EC 1.7.1.1) nia - Lotus japonicus
            gi|517356|emb|CAA56696.1| nitrate reductase (NADH) [Lotus
            japonicus]
          Length = 900

 Score =  209 bits (533), Expect = 1e-53
 Identities = 100/101 (99%), Positives = 100/101 (99%)
 Frame = -1

Query: 535  TKMYVV*ANRTEDDILLREELDTWAKKYEDRFKVWYVVETAKEGWGYSVGFVTEGVMREH 356
            TKMYVV ANRTEDDILLREELDTWAKKYEDRFKVWYVVETAKEGWGYSVGFVTEGVMREH
Sbjct: 800  TKMYVVYANRTEDDILLREELDTWAKKYEDRFKVWYVVETAKEGWGYSVGFVTEGVMREH 859

Query: 355  LPEAGDDALALACGPPPMIQFAVNPNLEKMGYDVKNDLLVF 233
            LPEAGDDALALACGPPPMIQFAVNPNLEKMGYDVKNDLLVF
Sbjct: 860  LPEAGDDALALACGPPPMIQFAVNPNLEKMGYDVKNDLLVF 900

>sp|P39866|NIA2_PHAVU Nitrate reductase [NADH] 2 (NR-2) gi|7432653|pir||T11805 nitrate
            reductase (NADH) (EC 1.7.1.1) 2 [similarity] - kidney
            bean gi|392992|gb|AAA95940.1| nitrate reductase
          Length = 890

 Score =  182 bits (462), Expect = 2e-45
 Identities = 83/101 (82%), Positives = 93/101 (91%)
 Frame = -1

Query: 535  TKMYVV*ANRTEDDILLREELDTWAKKYEDRFKVWYVVETAKEGWGYSVGFVTEGVMREH 356
            T+M+VV ANRTEDDILLREELDTWAK++ +RFKVWYVVETAKEGWGY VGF+TE +MREH
Sbjct: 790  TEMHVVYANRTEDDILLREELDTWAKEHCERFKVWYVVETAKEGWGYGVGFITEAIMREH 849

Query: 355  LPEAGDDALALACGPPPMIQFAVNPNLEKMGYDVKNDLLVF 233
            LPEA  D+LA+ CGPPPMIQFAV PNLEKMGYD+KNDLLVF
Sbjct: 850  LPEASSDSLAMTCGPPPMIQFAVQPNLEKMGYDIKNDLLVF 890

>sp|P43101|NIA_CICIN Nitrate reductase [NADH] (NR) gi|1086084|pir||S52301 nitrate
            reductase (NADH) (EC 1.7.1.1) - chicory
            gi|662902|emb|CAA58909.1| nitrate reductase (NADH)
            [Cichorium intybus]
          Length = 920

 Score =  168 bits (426), Expect = 3e-41
 Identities = 78/102 (76%), Positives = 88/102 (85%), Gaps = 1/102 (0%)
 Frame = -1

Query: 535  TKMYVV*ANRTEDDILLREELDTWAKKYEDRFKVWYVV-ETAKEGWGYSVGFVTEGVMRE 359
            T+MYVV ANRTEDDILLREELD WA KY DR KVWYVV ++ +EGW YS GF+TE +MRE
Sbjct: 819  TEMYVVYANRTEDDILLREELDAWADKYSDRVKVWYVVAKSIREGWKYSEGFITEDIMRE 878

Query: 358  HLPEAGDDALALACGPPPMIQFAVNPNLEKMGYDVKNDLLVF 233
            H+PE  +D LALACGPPPMIQFA+NPNLEKMGYD+KN LLVF
Sbjct: 879  HVPEVSEDTLALACGPPPMIQFAINPNLEKMGYDIKNSLLVF 920

>gb|AAG30576.1|AF314093_1 nitrate reductase [Ricinus communis]
          Length = 914

 Score =  168 bits (425), Expect = 4e-41
 Identities = 78/102 (76%), Positives = 89/102 (86%), Gaps = 1/102 (0%)
 Frame = -1

Query: 535  TKMYVV*ANRTEDDILLREELDTWAKKYEDRFKVWYVV-ETAKEGWGYSVGFVTEGVMRE 359
            T+MYVV ANRTEDDILLREELD+WAK++ +R KVWYVV E+ KEGW YSVGF+TE ++RE
Sbjct: 813  TEMYVVYANRTEDDILLREELDSWAKEHHERLKVWYVVQESIKEGWQYSVGFITENILRE 872

Query: 358  HLPEAGDDALALACGPPPMIQFAVNPNLEKMGYDVKNDLLVF 233
            H+PE  DD LALACGPPPMIQFAV PNLEKM YD+KN LLVF
Sbjct: 873  HVPEGSDDTLALACGPPPMIQFAVQPNLEKMNYDIKNSLLVF 914

>prf||1713435B nitrate reductase
          Length = 904

 Score =  160 bits (406), Expect = 7e-39
 Identities = 74/102 (72%), Positives = 88/102 (85%), Gaps = 1/102 (0%)
 Frame = -1

Query: 535  TKMYVV*ANRTEDDILLREELDTWAKKYEDRFKVWYVVETA-KEGWGYSVGFVTEGVMRE 359
            T+MYVV ANRTEDDILL+EELD+WA+K  +R KVWYVV+ + KEGW YS+GF+TE ++RE
Sbjct: 803  TEMYVVYANRTEDDILLKEELDSWAEKIPERVKVWYVVQDSIKEGWKYSIGFITEAILRE 862

Query: 358  HLPEAGDDALALACGPPPMIQFAVNPNLEKMGYDVKNDLLVF 233
            H+PE     LALACGPPPMIQFAVNPNLEKMGYD+K+ LLVF
Sbjct: 863  HIPEPSHTTLALACGPPPMIQFAVNPNLEKMGYDIKDSLLVF 904

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 523,276,439
Number of Sequences: 1393205
Number of extensions: 13602201
Number of successful extensions: 63345
Number of sequences better than 10.0: 373
Number of HSP's better than 10.0 without gapping: 46133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 59743
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17885181664
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENLf017f01 BP063276 1 533 2 GENLf039e08 BP064407 9 535

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