Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000455A_C01 KMC000455A_c01
(518 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_197525.1| glycosyl hydrolase family 36; protein id: At5g2... 116 2e-25
gb|AAL90901.1| AT5g20250/F5O24_140 [Arabidopsis thaliana] gi|233... 116 2e-25
pir||S27762 Sip1 protein - barley gi|167100|gb|AAA32975.1| seed ... 93 2e-18
gb|AAN32954.1| alkaline alpha-galactosidase seed imbibition prot... 88 7e-17
pir||S45033 probable imbibition protein - wild cabbage gi|488787... 75 3e-13
>ref|NP_197525.1| glycosyl hydrolase family 36; protein id: At5g20250.1 [Arabidopsis
thaliana]
Length = 749
Score = 116 bits (290), Expect = 2e-25
Identities = 53/108 (49%), Positives = 78/108 (72%)
Frame = -1
Query: 518 VSPVKDLAPGYRFAPVGLVNMFHAGGAVEGLAYEAVEGGSEGGLVGRVRLEIKGCGKFGA 339
VSP+ L G FAP+GLVNM+++GGA+EGL YEA + +V +E+KGCGKFG+
Sbjct: 650 VSPISHLVDGVSFAPIGLVNMYNSGGAIEGLRYEAEKM--------KVVMEVKGCGKFGS 701
Query: 338 YSSARPTRCLLGTDAVEFEYDSDSGLLSFVIDHLPREEEKVHHVQIEL 195
YSS +P RC++ ++ + FEYDS SGL++F +D +P E ++ H +Q+EL
Sbjct: 702 YSSVKPKRCVVESNEIAFEYDSSSGLVTFELDKMPIENKRFHLIQVEL 749
>gb|AAL90901.1| AT5g20250/F5O24_140 [Arabidopsis thaliana] gi|23308457|gb|AAN18198.1|
At5g20250/F5O24_140 [Arabidopsis thaliana]
Length = 844
Score = 116 bits (290), Expect = 2e-25
Identities = 53/108 (49%), Positives = 78/108 (72%)
Frame = -1
Query: 518 VSPVKDLAPGYRFAPVGLVNMFHAGGAVEGLAYEAVEGGSEGGLVGRVRLEIKGCGKFGA 339
VSP+ L G FAP+GLVNM+++GGA+EGL YEA + +V +E+KGCGKFG+
Sbjct: 745 VSPISHLVDGVSFAPIGLVNMYNSGGAIEGLRYEAEKM--------KVVMEVKGCGKFGS 796
Query: 338 YSSARPTRCLLGTDAVEFEYDSDSGLLSFVIDHLPREEEKVHHVQIEL 195
YSS +P RC++ ++ + FEYDS SGL++F +D +P E ++ H +Q+EL
Sbjct: 797 YSSVKPKRCVVESNEIAFEYDSSSGLVTFELDKMPIENKRFHLIQVEL 844
>pir||S27762 Sip1 protein - barley gi|167100|gb|AAA32975.1| seed imbibition
protein
Length = 757
Score = 92.8 bits (229), Expect = 2e-18
Identities = 50/108 (46%), Positives = 71/108 (65%)
Frame = -1
Query: 518 VSPVKDLAPGYRFAPVGLVNMFHAGGAVEGLAYEAVEGGSEGGLVGRVRLEIKGCGKFGA 339
V PV+ +APG FAP+GL++MF+AGGAVE VE G +G V V L ++GCG+FGA
Sbjct: 656 VCPVRAVAPGVSFAPIGLLHMFNAGGAVEECT---VETGEDGNAV--VGLRVRGCGRFGA 710
Query: 338 YSSARPTRCLLGTDAVEFEYDSDSGLLSFVIDHLPREEEKVHHVQIEL 195
Y S RP +C + + VEF YDSD+GL V +P E++++ +E+
Sbjct: 711 YCSRRPAKCSVDSADVEFTYDSDTGL---VTADVPVPEKEMYRCALEI 755
>gb|AAN32954.1| alkaline alpha-galactosidase seed imbibition protein [Lycopersicon
esculentum]
Length = 756
Score = 87.8 bits (216), Expect = 7e-17
Identities = 44/107 (41%), Positives = 68/107 (63%)
Frame = -1
Query: 518 VSPVKDLAPGYRFAPVGLVNMFHAGGAVEGLAYEAVEGGSEGGLVGRVRLEIKGCGKFGA 339
V P+ ++ G RFAP+GLVNMF++GGA++ + YE G G V ++++GCG FGA
Sbjct: 656 VVPINEMXTGSRFAPIGLVNMFNSGGAIKEVKYETE------GKCGLVSMKVRGCGTFGA 709
Query: 338 YSSARPTRCLLGTDAVEFEYDSDSGLLSFVIDHLPREEEKVHHVQIE 198
YSS +P R + + V+F+YD SGL + I +P +E + V++E
Sbjct: 710 YSSGKPKRIHVDNEEVQFDYDESSGLFTINIT-VPDQELYLWDVKVE 755
>pir||S45033 probable imbibition protein - wild cabbage
gi|488787|emb|CAA55893.1| putative imbibition protein
[Brassica oleracea]
Length = 765
Score = 75.5 bits (184), Expect = 3e-13
Identities = 40/119 (33%), Positives = 67/119 (55%), Gaps = 11/119 (9%)
Frame = -1
Query: 518 VSPVKDLAPGYRFAPVGLVNMFHAGGAVEGLAYEAVEGG----SEGGLVGRVR------- 372
+SP+K++ FAP+GL++MF++ GA++ + V S +V R
Sbjct: 648 ISPLKEITASISFAPIGLLDMFNSSGAIQSMEINTVTDEKPELSSSSVVSENRSPTALIS 707
Query: 371 LEIKGCGKFGAYSSARPTRCLLGTDAVEFEYDSDSGLLSFVIDHLPREEEKVHHVQIEL 195
L ++GCG+FGAYSS RP RC + EF YD++ GL++ +LP E++ ++E+
Sbjct: 708 LGVRGCGRFGAYSSQRPLRCAVDGTETEFNYDAEVGLVTL---NLPVTREEMFRWRVEI 763
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 489,654,094
Number of Sequences: 1393205
Number of extensions: 11229479
Number of successful extensions: 52576
Number of sequences better than 10.0: 159
Number of HSP's better than 10.0 without gapping: 42319
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 50856
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 16442828304
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
