Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000447A_C01 KMC000447A_c01
(669 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_176636.1| ABC transporter family protein; protein id: At1... 268 4e-71
pir||A96669 protein F1N19.11 [imported] - Arabidopsis thaliana g... 268 4e-71
ref|NP_116664.1| Positive effector of the EIF-2-alpha kinase act... 153 2e-36
gb|AAL87691.1|AF479253_1 non-transporter ABC protein AbcF1 [Dict... 152 4e-36
gb|EAA28430.1| hypothetical protein [Neurospora crassa] 148 7e-35
>ref|NP_176636.1| ABC transporter family protein; protein id: At1g64550.1 [Arabidopsis
thaliana] gi|25082978|gb|AAN72026.1| ABC transporter
protein, putative [Arabidopsis thaliana]
Length = 715
Score = 268 bits (686), Expect = 4e-71
Identities = 129/144 (89%), Positives = 136/144 (93%)
Frame = -3
Query: 667 VFSQHHVDGLDLSSNPLLYMMRCYPGVPEQKLRAHLGSFGVTGNLALQPMYTLSGAQKSR 488
VFSQHHVDGLDLSSNPLLYMMRCYPGVPEQKLR+HLGS GVTGNLALQPMYTLSG QKSR
Sbjct: 571 VFSQHHVDGLDLSSNPLLYMMRCYPGVPEQKLRSHLGSLGVTGNLALQPMYTLSGGQKSR 630
Query: 487 VAFAKITFKKPHIILLDEPSHHLDLDAVEALIQGLVMFQGGILMVSHDEHLISGSVDELW 308
VAFAKITFKKPH++LLDEPS+HLDLDAVEALIQGLV+FQGGI MVSHDEHLISGSVDELW
Sbjct: 631 VAFAKITFKKPHLLLLDEPSNHLDLDAVEALIQGLVLFQGGICMVSHDEHLISGSVDELW 690
Query: 307 VVSQGTATPFHGSFQDYKKILQSS 236
VVS G PFHG+F DYKK+LQSS
Sbjct: 691 VVSDGRIAPFHGTFHDYKKLLQSS 714
Score = 54.7 bits (130), Expect = 1e-06
Identities = 30/111 (27%), Positives = 57/111 (51%)
Frame = -3
Query: 583 EQKLRAHLGSFGVTGNLALQPMYTLSGAQKSRVAFAKITFKKPHIILLDEPSHHLDLDAV 404
E + + L T + L+ T SG + R+A A+ F +P ++LLDEP++HLDL AV
Sbjct: 316 EARAASILAGLSFTPEMQLKATNTFSGGWRMRIALARALFIEPDLLLLDEPTNHLDLHAV 375
Query: 403 EALIQGLVMFQGGILMVSHDEHLISGSVDELWVVSQGTATPFHGSFQDYKK 251
L L + ++VSH ++ V ++ + + + G++ +++
Sbjct: 376 LWLETYLTKWPKTFIVVSHAREFLNTVVTDIIHLQNQKLSTYKGNYDIFER 426
>pir||A96669 protein F1N19.11 [imported] - Arabidopsis thaliana
gi|6633814|gb|AAF19673.1|AC009519_7 F1N19.11 [Arabidopsis
thaliana]
Length = 1270
Score = 268 bits (686), Expect = 4e-71
Identities = 129/144 (89%), Positives = 136/144 (93%)
Frame = -3
Query: 667 VFSQHHVDGLDLSSNPLLYMMRCYPGVPEQKLRAHLGSFGVTGNLALQPMYTLSGAQKSR 488
VFSQHHVDGLDLSSNPLLYMMRCYPGVPEQKLR+HLGS GVTGNLALQPMYTLSG QKSR
Sbjct: 1126 VFSQHHVDGLDLSSNPLLYMMRCYPGVPEQKLRSHLGSLGVTGNLALQPMYTLSGGQKSR 1185
Query: 487 VAFAKITFKKPHIILLDEPSHHLDLDAVEALIQGLVMFQGGILMVSHDEHLISGSVDELW 308
VAFAKITFKKPH++LLDEPS+HLDLDAVEALIQGLV+FQGGI MVSHDEHLISGSVDELW
Sbjct: 1186 VAFAKITFKKPHLLLLDEPSNHLDLDAVEALIQGLVLFQGGICMVSHDEHLISGSVDELW 1245
Query: 307 VVSQGTATPFHGSFQDYKKILQSS 236
VVS G PFHG+F DYKK+LQSS
Sbjct: 1246 VVSDGRIAPFHGTFHDYKKLLQSS 1269
Score = 54.7 bits (130), Expect = 1e-06
Identities = 30/111 (27%), Positives = 57/111 (51%)
Frame = -3
Query: 583 EQKLRAHLGSFGVTGNLALQPMYTLSGAQKSRVAFAKITFKKPHIILLDEPSHHLDLDAV 404
E + + L T + L+ T SG + R+A A+ F +P ++LLDEP++HLDL AV
Sbjct: 802 EARAASILAGLSFTPEMQLKATNTFSGGWRMRIALARALFIEPDLLLLDEPTNHLDLHAV 861
Query: 403 EALIQGLVMFQGGILMVSHDEHLISGSVDELWVVSQGTATPFHGSFQDYKK 251
L L + ++VSH ++ V ++ + + + G++ +++
Sbjct: 862 LWLETYLTKWPKTFIVVSHAREFLNTVVTDIIHLQNQKLSTYKGNYDIFER 912
>ref|NP_116664.1| Positive effector of the EIF-2-alpha kinase activity of GCN2;
component of a heteromeric complex that includes GCN1 and
GCN20; Gcn20p [Saccharomyces cerevisiae]
gi|1169871|sp|P43535|GC20_YEAST GCN20 protein
gi|1363661|pir||S56146 GCN20 protein - yeast
(Saccharomyces cerevisiae) gi|643479|gb|AAA75444.1|
Gcn20p gi|836764|dbj|BAA09248.1| YFR009W [Saccharomyces
cerevisiae]
Length = 752
Score = 153 bits (386), Expect = 2e-36
Identities = 73/144 (50%), Positives = 100/144 (68%), Gaps = 1/144 (0%)
Frame = -3
Query: 664 FSQHHVDGLDLSSNPLLYMMRCYPGVPEQKLRAHLGSFGVTGNLALQPMYTLSGAQKSRV 485
F+QHHVD +DL+++ + +M + +PG +++ R HLGSFG+TG L LQ M LSG QKSRV
Sbjct: 600 FTQHHVDSMDLTTSAVDWMSKSFPGKTDEEYRRHLGSFGITGTLGLQKMQLLSGGQKSRV 659
Query: 484 AFAKITFKKPHIILLDEPSHHLDLDAVEALIQGLVMFQGGILMVSHDEHLISGSVDELWV 305
AFA + PHI++LDEPS+HLD ++AL++ L F GG+LMVSHD +I E+WV
Sbjct: 660 AFAALCLNNPHILVLDEPSNHLDTTGLDALVEALKNFNGGVLMVSHDISVIDSVCKEIWV 719
Query: 304 VSQGTATPFHGSFQDYKK-ILQSS 236
QGT F G+ DY+ ILQS+
Sbjct: 720 SEQGTVKRFEGTIYDYRDYILQSA 743
Score = 50.4 bits (119), Expect = 2e-05
Identities = 26/85 (30%), Positives = 46/85 (53%)
Frame = -3
Query: 583 EQKLRAHLGSFGVTGNLALQPMYTLSGAQKSRVAFAKITFKKPHIILLDEPSHHLDLDAV 404
E + + L G + QP + SG + R++ A+ F +P ++LLDEPS+ LD+ ++
Sbjct: 344 EARAASILYGLGFSTEAQQQPTNSFSGGWRMRLSLARALFCQPDLLLLDEPSNMLDVPSI 403
Query: 403 EALIQGLVMFQGGILMVSHDEHLIS 329
L + L + +L VSHD ++
Sbjct: 404 AYLAEYLKTYPNTVLTVSHDRAFLN 428
>gb|AAL87691.1|AF479253_1 non-transporter ABC protein AbcF1 [Dictyostelium discoideum]
Length = 708
Score = 152 bits (384), Expect = 4e-36
Identities = 77/145 (53%), Positives = 99/145 (68%), Gaps = 5/145 (3%)
Frame = -3
Query: 664 FSQHHVDGLDLSSNPLLYMMRCYPGVPEQKLRAHLGSFGVTGNLALQPMYTLSGAQKSRV 485
FSQH VD LDLS +PL + YPG Q R+HLG FG++G++AL+ + TLSG QKSRV
Sbjct: 561 FSQHFVDQLDLSKSPLDNFLAKYPGTTAQTARSHLGKFGLSGDIALRTVNTLSGGQKSRV 620
Query: 484 AFAKITFKKPHIILLDEPSHHLDLDAVEALIQGLVMFQGGILMVSHDEHLISGSVDELWV 305
A+I++ KPHI+LLDEPS+HLD+D V+AL L +F+GGIL+VSHDE LIS DE+W
Sbjct: 621 VLAQISYTKPHILLLDEPSNHLDIDTVDALCHALNVFEGGILLVSHDERLISLVCDEIWY 680
Query: 304 VSQGTATP-----FHGSFQDYKKIL 245
P F G + DYKK +
Sbjct: 681 FDGEEGEPKEVKNFDGDWNDYKKAI 705
Score = 57.4 bits (137), Expect = 2e-07
Identities = 30/111 (27%), Positives = 58/111 (52%)
Frame = -3
Query: 583 EQKLRAHLGSFGVTGNLALQPMYTLSGAQKSRVAFAKITFKKPHIILLDEPSHHLDLDAV 404
E + A L G T + QP + SG + R++ A+ F +P ++LLDEP++HLDL A
Sbjct: 306 ESRAAAILSGLGFTEEMQQQPTKSFSGGWRMRISLARALFIQPDVLLLDEPTNHLDLFAC 365
Query: 403 EALIQGLVMFQGGILMVSHDEHLISGSVDELWVVSQGTATPFHGSFQDYKK 251
L LV + +++VSH ++ ++ ++ + G++ +++
Sbjct: 366 LWLESYLVNWNRTLVIVSHQREFLNAVCTDIMHLNNKKIDYYKGNYSVFER 416
>gb|EAA28430.1| hypothetical protein [Neurospora crassa]
Length = 749
Score = 148 bits (373), Expect = 7e-35
Identities = 69/138 (50%), Positives = 95/138 (68%)
Frame = -3
Query: 664 FSQHHVDGLDLSSNPLLYMMRCYPGVPEQKLRAHLGSFGVTGNLALQPMYTLSGAQKSRV 485
F+QHHVD LDL+++ + +M + YPG +++ R LG+FG+TG LQ M LSG QKSRV
Sbjct: 597 FAQHHVDALDLNASAVSFMAKTYPGKTDEEYRRQLGAFGITGTTGLQKMALLSGGQKSRV 656
Query: 484 AFAKITFKKPHIILLDEPSHHLDLDAVEALIQGLVMFQGGILMVSHDEHLISGSVDELWV 305
AFA + +PHI++LDEPS+HLD++A++AL + L F+GG+LMVSHD ++ LWV
Sbjct: 657 AFACLALTQPHILVLDEPSNHLDIEAMDALSEALQKFEGGVLMVSHDVTMLQTVCKSLWV 716
Query: 304 VSQGTATPFHGSFQDYKK 251
GT F G Q YKK
Sbjct: 717 CENGTVEKFPGDVQAYKK 734
Score = 48.1 bits (113), Expect = 1e-04
Identities = 23/62 (37%), Positives = 38/62 (61%)
Frame = -3
Query: 514 TLSGAQKSRVAFAKITFKKPHIILLDEPSHHLDLDAVEALIQGLVMFQGGILMVSHDEHL 335
T SG + R+A A+ F +P ++LLDEPS+ LD+ ++ L L + +L+VSHD
Sbjct: 364 TFSGGWRMRLALARALFCEPDLLLLDEPSNMLDVPSITFLSNYLQTYPSTVLVVSHDRAF 423
Query: 334 IS 329
++
Sbjct: 424 LN 425
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 547,191,329
Number of Sequences: 1393205
Number of extensions: 11623864
Number of successful extensions: 42674
Number of sequences better than 10.0: 7250
Number of HSP's better than 10.0 without gapping: 39693
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 42337
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 29138478756
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
