KMC000440A_c02
[Fasta Sequence]
[Nr Search]
[EST assemble image]
Fasta Sequence
>KMC000440A_C02 KMC000440A_c02
ataacaacgaaatAAAATGAAACTAAAAAAACTTCACCTCATTTTCCACATTTGGCAATG
GGTATCACATTAATATCATATTTATGAATTGTTGAATGTTTAAGCAGAAAACAGGTAAAT
GGGGAAGGGGCAACTCACCCCCATAATTAGATTAGATGAACTTACAACGTCAGATTTCCG
CTAGACTCAACAAAACAGGGAAAAAAAAATTATAAAAAAAGAAATGAGTAAAAAAAGAAA
TGTTTTATGCCTCCATTCTACAGATCTCAGAATCTGAAGTCAGGGTCCACATCCATAGCC
CAAGTGAACTTGGCCAGCAGAGACTGGTTGAAAATCTTTTCAATAGGAACCTCGTGTCTC
TTGCACCAGGCGACTGTAATCCTGGGATCAAGGTAGTTTATCTTGGATGTGCCTAATGCC
ACAGTTTTGAGATCTTCTTTTGTCTTCATATCACGCTGCATTTTCTCAATCTTTGCATTG
GTTTGAGATATCTTTTTCTCTAGCGCTTCGGAAGACAAGTTCTTCTTCTTCTTTCCATCT
GGACTCTTTGAAGGGGGCTTTCCTTTCCTAGCCCTGTCCAAATCCGTTCTTAGCTCCTTC
AGAACAGCCTGAAGTTCATCAAGCTTTTCATTTAAC
Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000440A_C02 KMC000440A_c02
(636 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAB46363.1| DNA topoisomerase I [Daucus carota] 193 2e-48
ref|NP_200342.1| DNA topoisomerase I; protein id: At5g55310.1 [A... 190 1e-47
gb|AAL87337.2| putative DNA topoisomerase I [Arabidopsis thaliana] 190 1e-47
pir||T06818 DNA topoisomerase (EC 5.99.1.2) I - garden pea gi|23... 182 4e-47
ref|NP_200341.1| DNA topoisomerase I (sp P30181); protein id: At... 186 2e-46
>emb|CAB46363.1| DNA topoisomerase I [Daucus carota]
Length = 909
Score = 193 bits (490), Expect = 2e-48
Identities = 90/122 (73%), Positives = 110/122 (89%)
Frame = -2
Query: 635 LNEKLDELQAVLKELRTDLDRARKGKPPSKSPDGKKKKNLSSEALEKKISQTNAKIEKMQ 456
L+EK+++L+ ++EL+TDL RA+KGKPP K DGK K+N++ EA+EKKI+QTN KIEKM+
Sbjct: 788 LDEKINKLKNFIEELKTDLSRAKKGKPPLKGSDGKTKRNMNPEAIEKKIAQTNVKIEKME 847
Query: 455 RDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNQSLLAKFTWAMDVDPDF 276
RD +TKEDLKTVALGTSKINYLDPRITVAWCKR+EVPIEK+FN+SLLAKF WAMDV+P F
Sbjct: 848 RDKETKEDLKTVALGTSKINYLDPRITVAWCKRYEVPIEKMFNKSLLAKFAWAMDVEPSF 907
Query: 275 RF 270
RF
Sbjct: 908 RF 909
>ref|NP_200342.1| DNA topoisomerase I; protein id: At5g55310.1 [Arabidopsis thaliana]
gi|9758163|dbj|BAB08548.1| DNA topoisomerase I
[Arabidopsis thaliana]
Length = 917
Score = 190 bits (483), Expect = 1e-47
Identities = 89/119 (74%), Positives = 105/119 (87%)
Frame = -2
Query: 626 KLDELQAVLKELRTDLDRARKGKPPSKSPDGKKKKNLSSEALEKKISQTNAKIEKMQRDM 447
K++EL+ +KEL DLDRA+KG+ P DGK+K+NL+ EALEKKI QT KIEKM+RDM
Sbjct: 793 KIEELREQIKELNIDLDRAKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDM 852
Query: 446 KTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNQSLLAKFTWAMDVDPDFRF 270
+TKED+KTVALGTSKINY+DPRITVAWCKRH+VPIEKIFN+SLLAKF WAMDVDP+FRF
Sbjct: 853 QTKEDMKTVALGTSKINYMDPRITVAWCKRHDVPIEKIFNKSLLAKFAWAMDVDPEFRF 911
>gb|AAL87337.2| putative DNA topoisomerase I [Arabidopsis thaliana]
Length = 232
Score = 190 bits (483), Expect = 1e-47
Identities = 89/119 (74%), Positives = 105/119 (87%)
Frame = -2
Query: 626 KLDELQAVLKELRTDLDRARKGKPPSKSPDGKKKKNLSSEALEKKISQTNAKIEKMQRDM 447
K++EL+ +KEL DLDRA+KG+ P DGK+K+NL+ EALEKKI QT KIEKM+RDM
Sbjct: 108 KIEELREQIKELNIDLDRAKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDM 167
Query: 446 KTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNQSLLAKFTWAMDVDPDFRF 270
+TKED+KTVALGTSKINY+DPRITVAWCKRH+VPIEKIFN+SLLAKF WAMDVDP+FRF
Sbjct: 168 QTKEDMKTVALGTSKINYMDPRITVAWCKRHDVPIEKIFNKSLLAKFAWAMDVDPEFRF 226
>pir||T06818 DNA topoisomerase (EC 5.99.1.2) I - garden pea
gi|2330649|emb|CAA74890.1| topoisomerase I [Pisum
sativum]
Length = 892
Score = 182 bits (461), Expect(2) = 4e-47
Identities = 90/107 (84%), Positives = 98/107 (91%)
Frame = -2
Query: 635 LNEKLDELQAVLKELRTDLDRARKGKPPSKSPDGKKKKNLSSEALEKKISQTNAKIEKMQ 456
LNEK+DELQ VLKEL+ DLDRARKGK P+KS DGK K++L+ E LEKKISQTNAKIEKMQ
Sbjct: 766 LNEKIDELQDVLKELKVDLDRARKGKSPTKSSDGKSKRSLTPEVLEKKISQTNAKIEKMQ 825
Query: 455 RDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNQSLL 315
RDMKTK DLKTVALGTSKINYLDPRITVAWCKRHEVPIEK+F +SLL
Sbjct: 826 RDMKTKVDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKLFTKSLL 872
Score = 28.5 bits (62), Expect(2) = 4e-47
Identities = 15/26 (57%), Positives = 17/26 (64%)
Frame = -3
Query: 340 KRFSTSLCWPSSLGLWMWTLTSDSEI 263
K F+ SL + LG WMWT SDSEI
Sbjct: 865 KLFTKSLL-QNLLGQWMWTQISDSEI 889
>ref|NP_200341.1| DNA topoisomerase I (sp P30181); protein id: At5g55300.1 [Arabidopsis
thaliana] gi|267146|sp|P30181|TOP1_ARATH DNA
topoisomerase I gi|99762|pir||S22864 DNA topoisomerase
(EC 5.99.1.2) I - Arabidopsis thaliana
gi|16558|emb|CAA40763.1| topoisomerase I [Arabidopsis
thaliana] gi|9758162|dbj|BAB08547.1| DNA topoisomerase I
[Arabidopsis thaliana] gi|445137|prf||1908437A
topoisomerase I
Length = 916
Score = 186 bits (473), Expect = 2e-46
Identities = 89/122 (72%), Positives = 106/122 (85%)
Frame = -2
Query: 635 LNEKLDELQAVLKELRTDLDRARKGKPPSKSPDGKKKKNLSSEALEKKISQTNAKIEKMQ 456
L +++EL+ VLKEL+T+LDRA+KGKPP + DGKK ++L A EKKI+Q +AKIEKM+
Sbjct: 792 LTARIEELKEVLKELKTNLDRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKME 851
Query: 455 RDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNQSLLAKFTWAMDVDPDF 276
RDM TKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIF +SLL KF WAMDV+P++
Sbjct: 852 RDMHTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFTKSLLEKFAWAMDVEPEY 911
Query: 275 RF 270
RF
Sbjct: 912 RF 913
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 538,544,270
Number of Sequences: 1393205
Number of extensions: 11544584
Number of successful extensions: 37910
Number of sequences better than 10.0: 178
Number of HSP's better than 10.0 without gapping: 35417
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37709
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 26439068301
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
EST assemble image
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clone |
accession |
position |
| 1 |
MPDL057c12_f |
AV779388 |
1 |
549 |
| 2 |
SPDL022c11_f |
BP053365 |
2 |
553 |
| 3 |
MRL020b07_f |
BP084729 |
14 |
395 |
| 4 |
MF036e12_f |
BP030213 |
19 |
472 |
| 5 |
MPDL014f07_f |
AV777236 |
19 |
427 |
| 6 |
GENLf076g05 |
BP066491 |
21 |
565 |
| 7 |
MPDL087h08_f |
AV781078 |
25 |
593 |
| 8 |
MFL005d02_f |
BP033615 |
25 |
569 |
| 9 |
SPDL067h11_f |
BP056193 |
26 |
551 |
| 10 |
SPDL010a08_f |
BP052566 |
39 |
556 |
| 11 |
GENLf033d12 |
BP064072 |
57 |
551 |
| 12 |
GENLf016g08 |
BP063232 |
63 |
567 |
| 13 |
SPDL043b11_f |
BP054694 |
64 |
556 |
| 14 |
MFBL007f01_f |
BP041633 |
65 |
645 |
| 15 |
SPDL090h02_f |
BP057671 |
66 |
640 |
| 16 |
SPDL026b05_f |
BP053589 |
68 |
550 |
| 17 |
MRL031h07_f |
BP085278 |
72 |
557 |
| 18 |
GENLf021b02 |
BP063443 |
121 |
565 |
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Lotus japonicus
Kazusa DNA Research Institute