>KMC000438A_C01 KMC000438A_c01
gtatctcatctaagaatgatttttCATGAAATAAAAATATACACTCAAAATTGATTTTAA
CAGTAAAAAATTAATTCTAATATACACAAACGGGCATGCTCTAATAAATTTAATTAGACT
CTTCATTTGCCAAACTCAATTCACTAAAAAAAGACCATGGATCCATCATGCCTAATACTC
CATAGGAATTTGGAGAGGAAAATTAGCAAGAAGCAGGGTGTGTGGTGTAAATGGAACTCT
TATAAATGTGTTGGGTAGAAACCATTCACGGACATTCCACCATCAACACATATAATCTGG
CCAGTGATGTATGATGATGCTGGTAAGCAAAGAAAGGCAACAAGAGAAGACACTTCTGCA
GGATCTCCGAGGCGCCTAAGAGGAGTTCGAGAGTAAACCTCTTCCAGATAGTCCTTGTGT
GCTGAGAACCTAAATGAAGAGGGGGAAAGTCAGATAAATGTTTATATCAGATnGCTGCAT
TAGAACATTATGCAAGTTCCAACCAAGATTCCAAATTCAATTCAATAAACCAAGCTGT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000438A_C01 KMC000438A_c01
         (538 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAO42159.1| putative tropinone reductase [Arabidopsis thaliana]    103  2e-21
ref|NP_180489.1| putative tropinone reductase; protein id: At2g2...   103  2e-21
pir||E84695 probable tropinone reductase [imported] - Arabidopsi...    86  3e-16
ref|NP_180498.2| putative tropinone reductase; protein id: At2g2...    86  3e-16
gb|AAM61117.1| short chain alcohol dehydrogenase-like [Arabidops...    86  4e-16

>gb|AAO42159.1| putative tropinone reductase [Arabidopsis thaliana]
          Length = 312

 Score =  103 bits (256), Expect = 2e-21
 Identities = 47/59 (79%), Positives = 54/59 (90%)
 Frame = -3

Query: 422 AHKDYLEEVYSRTPLRRLGDPAEVSSLVAFLCLPASSYITGQIICVDGGMSVNGFYPTH 246
           ++K+YLEEVYS TPL RLG+P EVSS VAFLCLPASSYITGQI+CVDGGMS+NGF+P H
Sbjct: 253 SNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSYITGQILCVDGGMSINGFFPRH 311

>ref|NP_180489.1| putative tropinone reductase; protein id: At2g29260.1 [Arabidopsis
           thaliana] gi|25283419|pir||D84694 probable tropinone
           reductase [imported] - Arabidopsis thaliana
           gi|3980406|gb|AAC95209.1| putative tropinone reductase
           [Arabidopsis thaliana]
          Length = 322

 Score =  103 bits (256), Expect = 2e-21
 Identities = 47/59 (79%), Positives = 54/59 (90%)
 Frame = -3

Query: 422 AHKDYLEEVYSRTPLRRLGDPAEVSSLVAFLCLPASSYITGQIICVDGGMSVNGFYPTH 246
           ++K+YLEEVYS TPL RLG+P EVSS VAFLCLPASSYITGQI+CVDGGMS+NGF+P H
Sbjct: 263 SNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSYITGQILCVDGGMSINGFFPRH 321

>pir||E84695 probable tropinone reductase [imported] - Arabidopsis thaliana
           gi|3980398|gb|AAC95201.1| putative tropinone reductase
           [Arabidopsis thaliana]
          Length = 268

 Score = 85.9 bits (211), Expect = 3e-16
 Identities = 42/63 (66%), Positives = 50/63 (78%)
 Frame = -3

Query: 446 SPSSFRFSAHKDYLEEVYSRTPLRRLGDPAEVSSLVAFLCLPASSYITGQIICVDGGMSV 267
           +PS+  F   KD  E+V S TPLRR+G+  EVSSLVAFLCLPA+SYITGQ ICVDGG ++
Sbjct: 203 TPSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAASYITGQTICVDGGFTI 262

Query: 266 NGF 258
           NGF
Sbjct: 263 NGF 265

>ref|NP_180498.2| putative tropinone reductase; protein id: At2g29370.1, supported by
           cDNA: gi_19424098 [Arabidopsis thaliana]
          Length = 183

 Score = 85.9 bits (211), Expect = 3e-16
 Identities = 42/63 (66%), Positives = 50/63 (78%)
 Frame = -3

Query: 446 SPSSFRFSAHKDYLEEVYSRTPLRRLGDPAEVSSLVAFLCLPASSYITGQIICVDGGMSV 267
           +PS+  F   KD  E+V S TPLRR+G+  EVSSLVAFLCLPA+SYITGQ ICVDGG ++
Sbjct: 118 TPSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAASYITGQTICVDGGFTI 177

Query: 266 NGF 258
           NGF
Sbjct: 178 NGF 180

>gb|AAM61117.1| short chain alcohol dehydrogenase-like [Arabidopsis thaliana]
          Length = 264

 Score = 85.5 bits (210), Expect = 4e-16
 Identities = 40/53 (75%), Positives = 47/53 (88%)
 Frame = -3

Query: 416 KDYLEEVYSRTPLRRLGDPAEVSSLVAFLCLPASSYITGQIICVDGGMSVNGF 258
           K+++E V SRTPL R+G+P EVSSLVAFLCLPASSYITGQ+I VDGG +VNGF
Sbjct: 206 KEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQVISVDGGFTVNGF 258

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 443,651,168
Number of Sequences: 1393205
Number of extensions: 9356703
Number of successful extensions: 23946
Number of sequences better than 10.0: 1673
Number of HSP's better than 10.0 without gapping: 23502
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23905
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 18173652336
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENLf016f11 BP063226 1 444 2 GENLf046b08 BP064763 25 539

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