>KMC000424A_C01 KMC000424A_c01
gatcaaataacttttcaattagggtttaataatattcatatcaagcaagtctgcttgaaa
aaagtaaaaagagagctctcGAAATTCTGCTTCTAAAAGTGCATGCAAAGAGAATTCAAT
ATGACTGCAAACAGTCCAAAATTTAAAGAGATTTAACAATGGATCTTAGCTGCCAAATCC
AAGAGTACATGATCCCAAAAGCACAGTATATAGAGATCAACATAAGGGGCGCGAATGCAA
GCTTATCACCCAAAAGAAGAGGGTGTAAAAATTGGCCCCATATCTCTACAACTACAAGAC
CAACCAGATAAATCATCTCAATCCAGCTAATAACAAAACCTTCATTCTTCACAGGTTCAC
CTGAACTACCTTTAGATCCAAATTGATCAACCTTTTTCTTTGGACGAGCATTTGACGCTC
TTGTCTCAATCCCATCATGAAATTGTGCTGAAAAGCCTGACCACATCAAAATGGAGTGCA
GAAGCAGCAACAGAACTTTGATAGGATACTCTTGTGCCTCGAATAGTAGCGGAAAAAGTG
AATAGCAGGATACTATTGATAGCAAAAAGTAATGCTTTGCATCCTCCAGGGTTTGCGCAG
CAACAATAGCTAGTGGAATTACAAAATGGAGTGATGCCTTCTCATGCACGTGCCACCCAA
AAAGAAAGCCACAGGTATAAGCATAGGCT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000424A_C01 KMC000424A_c01
         (689 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAO52349.1| similar to Homo sapiens (Human). Probable dolichy...   114  1e-24
ref|NP_181994.1| putative glucosyltransferase; protein id: At2g4...   114  2e-24
emb|CAA12176.1| glucosyltransferase [Homo sapiens]                    103  2e-21
ref|XP_214992.1| similar to hypothetical protein MGC2840 similar...   103  3e-21
ref|NP_076984.1| hypothetical protein MGC2840 similar to a putat...   102  7e-21

>gb|AAO52349.1| similar to Homo sapiens (Human). Probable dolichyl pyrophosphate
           Glc1Man9GlcNAc2 alpha-1,3- glucosyltransferase (EC
           2.4.1.-) (Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP- dolichyl
           glucosyltransferase) (HUSSY-2) [Dictyostelium
           discoideum]
          Length = 291

 Score =  114 bits (285), Expect = 1e-24
 Identities = 56/175 (32%), Positives = 102/175 (58%), Gaps = 2/175 (1%)
 Frame = -2

Query: 667 FLFGWHVHEKASLHFVIPLAIVAAQTLEDAKHYFLLSIVSCYSLFPLLFEAQEYPIKVLL 488
           F+FGWHVHEKA +   IP+  +     + +K YFLLS +  YSLFPLLF+ +E P ++L+
Sbjct: 122 FMFGWHVHEKAIIMITIPMGFLCLVNNKFSKLYFLLSTIGHYSLFPLLFQVEEIPTRILI 181

Query: 487 LLLHSILMWSGFSAQFHDGIETRASNARPKKKVDQFGSKGSSGEPVKNEGFVISWIEMIY 308
           L+ +++L++  F       I + ++N       +   +  +S +   +    + W E  Y
Sbjct: 182 LVTYTLLVYLSF-------ISSSSNNNNNNNNNNNNSNNNNSKQRKCSFSLGLYWFEKFY 234

Query: 307 LVGLVVVEIWGQFLHPLLLG--DKLAFAPLMLISIYCAFGIMYSWIWQLRSIVKS 149
           L G++++EI+  F+HP+ L   ++ +F  LM+ S+Y + GI+Y +++  + I K+
Sbjct: 235 LFGIILLEIFNVFIHPVFLAHIERFSFISLMITSVYTSVGIIYCYLFTFKLIFKN 289

>ref|NP_181994.1| putative glucosyltransferase; protein id: At2g44660.1 [Arabidopsis
           thaliana] gi|21263371|sp|O80505|ALG8_ARATH Probable
           dolichyl pyrophosphate Glc1Man9GlcNAc2
           alpha-1,3-glucosyltransferase
           (Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl
           glucosyltransferase) gi|7484991|pir||T01593
           glycosyltransferase homolog F16B22.15 - Arabidopsis
           thaliana gi|3341686|gb|AAC27468.1| putative
           glucosyltransferase [Arabidopsis thaliana]
          Length = 383

 Score =  114 bits (284), Expect = 2e-24
 Identities = 51/57 (89%), Positives = 55/57 (96%)
 Frame = -2

Query: 688 AYAYTCGFLFGWHVHEKASLHFVIPLAIVAAQTLEDAKHYFLLSIVSCYSLFPLLFE 518
           AYAYTCGFLFGWHVHEKASLHF IPLAIVA Q+LEDAKHYFL+SIVSCYSL+PLL+E
Sbjct: 311 AYAYTCGFLFGWHVHEKASLHFTIPLAIVAVQSLEDAKHYFLVSIVSCYSLYPLLYE 367

 Score = 35.0 bits (79), Expect = 0.98
 Identities = 15/42 (35%), Positives = 23/42 (54%)
 Frame = -3

Query: 594 KPWRMQSITFCYQ*YPAIHFFRYYSRHKSILSKFCCCFCTPF 469
           K + + SI  CY  YP ++        +S  S+FCCC+C P+
Sbjct: 348 KHYFLVSIVSCYSLYPLLY------EPRSTRSRFCCCYCIPW 383

>emb|CAA12176.1| glucosyltransferase [Homo sapiens]
          Length = 532

 Score =  103 bits (257), Expect = 2e-21
 Identities = 58/164 (35%), Positives = 85/164 (51%), Gaps = 1/164 (0%)
 Frame = -2

Query: 667 FLFGWHVHEKASLHFVIPLAIVAAQTLEDAKHYFLLSIVSCYSLFPLLFEAQEYPIKVLL 488
           F+FGWHVHEKA L  ++P+++++     DA  + +L+    YSLFPLLF A E PIK+LL
Sbjct: 380 FMFGWHVHEKAILLAILPMSLLSVGKAGDASIFLILTTTGHYSLFPLLFTAPELPIKILL 439

Query: 487 LLLHSILMWSGFSAQFHDGIETRASNARPKKKVDQFGSKGSSGEPVKNEGFVISWIEMIY 308
           +LL +I   S     F                              + E  + +W+E  Y
Sbjct: 440 MLLFTIYSISSLKTLF------------------------------RKEKPLFNWMETFY 469

Query: 307 LVGLVVVEIWGQFLHPLLLGD-KLAFAPLMLISIYCAFGIMYSW 179
           L+GL  +E+  +F++P      K  F PL+L S+YCA GI Y+W
Sbjct: 470 LLGLGPLEVCCEFVYPFTSWKVKYPFIPLLLTSVYCAVGITYAW 513

>ref|XP_214992.1| similar to hypothetical protein MGC2840 similar to a putative
           glucosyltransferase [Homo sapiens] [Rattus norvegicus]
          Length = 152

 Score =  103 bits (256), Expect = 3e-21
 Identities = 59/171 (34%), Positives = 88/171 (50%), Gaps = 1/171 (0%)
 Frame = -2

Query: 664 LFGWHVHEKASLHFVIPLAIVAAQTLEDAKHYFLLSIVSCYSLFPLLFEAQEYPIKVLLL 485
           +FGWHVHEKA L  ++P++++A +   DA  + +L+    YSLFPLLF A E PIK+LL+
Sbjct: 1   MFGWHVHEKAILLAILPMSLLAVEKAGDATIFLILTTTGHYSLFPLLFTAPELPIKILLM 60

Query: 484 LLHSILMWSGFSAQFHDGIETRASNARPKKKVDQFGSKGSSGEPVKNEGFVISWIEMIYL 305
            L ++   S     F                              + E  + +W+E +YL
Sbjct: 61  SLFTVYSISSLRTLF------------------------------RKEKPLFNWMETLYL 90

Query: 304 VGLVVVEIWGQFLHPLLLGD-KLAFAPLMLISIYCAFGIMYSWIWQLRSIV 155
           +GL  +E+  +FL P      K  F PL+L S+YCA GI Y+WI    S++
Sbjct: 91  LGLGPLEVCCEFLFPFTSWKLKYPFIPLLLTSVYCAVGITYAWIRLYASVL 141

>ref|NP_076984.1| hypothetical protein MGC2840 similar to a putative
           glucosyltransferase [Homo sapiens]
           gi|21263375|sp|Q9BVK2|ALG8_HUMAN Probable dolichyl
           pyrophosphate Glc1Man9GlcNAc2
           alpha-1,3-glucosyltransferase
           (Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl
           glucosyltransferase) (HUSSY-2)
           gi|12654595|gb|AAH01133.1|AAH01133 Unknown (protein for
           MGC:2840) [Homo sapiens]
          Length = 526

 Score =  102 bits (253), Expect = 7e-21
 Identities = 57/164 (34%), Positives = 84/164 (50%), Gaps = 1/164 (0%)
 Frame = -2

Query: 667 FLFGWHVHEKASLHFVIPLAIVAAQTLEDAKHYFLLSIVSCYSLFPLLFEAQEYPIKVLL 488
           F+FGWHVHEKA L  ++P+++++     DA  + +L+    YSLFPLLF A E PIK+LL
Sbjct: 374 FMFGWHVHEKAILLAILPMSLLSVGKAGDASIFLILTTTGHYSLFPLLFTAPELPIKILL 433

Query: 487 LLLHSILMWSGFSAQFHDGIETRASNARPKKKVDQFGSKGSSGEPVKNEGFVISWIEMIY 308
           +LL +I   S     F                              + E  + +W+E  Y
Sbjct: 434 MLLFTIYSISSLKTLF------------------------------RKEKPLFNWMETFY 463

Query: 307 LVGLVVVEIWGQFLHPLLLGD-KLAFAPLMLISIYCAFGIMYSW 179
           L+GL  +E+  +F+ P      K  F PL+L S+YCA G+ Y+W
Sbjct: 464 LLGLGPLEVCCEFVFPFTSWKVKYPFIPLLLTSVYCAVGVTYAW 507

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 568,492,528
Number of Sequences: 1393205
Number of extensions: 11701203
Number of successful extensions: 30854
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 29904
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30832
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 31118763720
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENLf016b10 BP063188 1 458 2 MPD020a11_f AV771362 93 589 3 MF069a01_f BP031945 128 531 4 MF090h11_f BP033046 150 691

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