>KMC000416A_C01 KMC000416A_c01
attgaaaagtttccTGCAAATTTTGATGTTGCATATATTTTCACAGCTACAGTATAGATC
GAAGGGGGTTACAAATATGCAAACCAGGAACAGGTACCCAATACCCATCCCTTCACTCAA
AGGAGAAAGCAGCTCCTCTCTTATATTTATGCATACTTATTATAAGATAAACTAATAAGG
CAGGGTTAAGATAAAGAACCTCCCGCATTTGTGTATGGAGAAGGTAAAAACACTTATTAT
TATGATGCATCGTGTTCTAAAGAGGGAGGATCACGACACTACGAAGTTAATTTGACATTA
AAACAGTGATATACACAAACAGATCACAAGCTCTAAAACAGGAAACCTCTAGTTTCCAAC
CCTCATTTTTTGTGATGGAACCTCACCTTCAATAAGAATTGCATCTTGACTTGAGCAGTA
TCATAGACAATATACTCATGGCACTGGGGTTCAGATCCCTCGACATTTGATGACACTGGT
TGGCCACAAGGAACGGTGACATCACCCCTCCACTTTATATGTTCTGATTCCAGAGGCATT
TCCTGGCCCAGTGCTTTAGTAGAGTGCTTTCCTTCTGGAGCTATGTCCATATACTCTTTT
TCTTTGATGACTTCATAGATATCTCCAAGGGCAACTTCACTCAAAAGCATTAGACCAATA
GGGTTTTTCTTATCCGTGTTGCAATATTGAGCACTCATGTGGACCAGGTCAGCAAAGTAA
ACC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000416A_C01 KMC000416A_c01
         (723 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||C84719 probable poly (ADP-ribose) polymerase [imported] - A...   172  3e-42
ref|NP_180690.2| putative poly (ADP-ribose) polymerase; protein ...   172  3e-42
gb|AAC79704.1| poly(ADP)-ribose polymerase [Zea mays]                 167  2e-40
pir||T03657 NAD ADP-ribosyltransferase (EC 2.4.2.30) 2 - maize g...   167  2e-40
dbj|BAC20853.1| putative NAD+ ADP-ribosyltransferase [Oryza sati...   164  9e-40

>pir||C84719 probable poly (ADP-ribose) polymerase [imported] - Arabidopsis
            thaliana gi|4432827|gb|AAD20677.1| putative poly
            (ADP-ribose) polymerase [Arabidopsis thaliana]
          Length = 1009

 Score =  172 bits (437), Expect = 3e-42
 Identities = 85/119 (71%), Positives = 99/119 (82%)
 Frame = -2

Query: 722  VYFADLVHMSAQYCNTDKKNPIGLMLLSEVALGDIYEVIKEKEYMDIAPEGKHSTKALGQ 543
            +YFADLV  SAQYC T KKNP+GLMLLSEVALG+I+E+ K K YMD  P GKHSTK LG+
Sbjct: 892  IYFADLVSKSAQYCYTCKKNPVGLMLLSEVALGEIHELTKAK-YMDKPPRGKHSTKGLGK 950

Query: 542  EMPLESEHIKWRGDVTVPCGQPVSSNVEGSEPQCHEYIVYDTAQVKMQFLLKVRFHHKK 366
            ++P +SE  KWRGDVTVPCG+PVSS V+ SE   +EYIVYDTAQVK+QFLLKVRF HK+
Sbjct: 951  KVPQDSEFAKWRGDVTVPCGKPVSSKVKASELMYNEYIVYDTAQVKLQFLLKVRFKHKR 1009

>ref|NP_180690.2| putative poly (ADP-ribose) polymerase; protein id: At2g31320.1,
            supported by cDNA: gi_20259523 [Arabidopsis thaliana]
            gi|11258415|pir||T51353 NAD ADP-ribosyltransferase (EC
            2.4.2.30) [imported] - Arabidopsis thaliana
            gi|4038491|emb|CAA10482.1| poly(ADP-ribose) polymerase
            [Arabidopsis thaliana] gi|20259524|gb|AAM13882.1|
            putative poly (ADP-ribose) polymerase [Arabidopsis
            thaliana] gi|23297589|gb|AAN12901.1| putative
            poly(ADP-ribose) polymerase [Arabidopsis thaliana]
          Length = 983

 Score =  172 bits (437), Expect = 3e-42
 Identities = 85/119 (71%), Positives = 99/119 (82%)
 Frame = -2

Query: 722  VYFADLVHMSAQYCNTDKKNPIGLMLLSEVALGDIYEVIKEKEYMDIAPEGKHSTKALGQ 543
            +YFADLV  SAQYC T KKNP+GLMLLSEVALG+I+E+ K K YMD  P GKHSTK LG+
Sbjct: 866  IYFADLVSKSAQYCYTCKKNPVGLMLLSEVALGEIHELTKAK-YMDKPPRGKHSTKGLGK 924

Query: 542  EMPLESEHIKWRGDVTVPCGQPVSSNVEGSEPQCHEYIVYDTAQVKMQFLLKVRFHHKK 366
            ++P +SE  KWRGDVTVPCG+PVSS V+ SE   +EYIVYDTAQVK+QFLLKVRF HK+
Sbjct: 925  KVPQDSEFAKWRGDVTVPCGKPVSSKVKASELMYNEYIVYDTAQVKLQFLLKVRFKHKR 983

>gb|AAC79704.1| poly(ADP)-ribose polymerase [Zea mays]
          Length = 980

 Score =  167 bits (422), Expect = 2e-40
 Identities = 80/119 (67%), Positives = 96/119 (80%)
 Frame = -2

Query: 722  VYFADLVHMSAQYCNTDKKNPIGLMLLSEVALGDIYEVIKEKEYMDIAPEGKHSTKALGQ 543
            +YFADLV  SAQYC  D+ NP+GLMLLSEVALGD+YE +K+   MD  P GKHSTK LG+
Sbjct: 863  LYFADLVSKSAQYCYVDRNNPVGLMLLSEVALGDMYE-LKKATSMDKPPRGKHSTKGLGK 921

Query: 542  EMPLESEHIKWRGDVTVPCGQPVSSNVEGSEPQCHEYIVYDTAQVKMQFLLKVRFHHKK 366
             +PLESE +KWR DV VPCG+PV S++  SE   +EYIVY+T+QVKMQFLLKVRFHHK+
Sbjct: 922  TVPLESEFVKWRDDVVVPCGKPVPSSIRSSELMYNEYIVYNTSQVKMQFLLKVRFHHKR 980

>pir||T03657 NAD ADP-ribosyltransferase (EC 2.4.2.30) 2 - maize
            gi|2632129|emb|CAA10889.1| poly(ADP-ribose) polymerase
            [Zea mays]
          Length = 969

 Score =  167 bits (422), Expect = 2e-40
 Identities = 80/119 (67%), Positives = 96/119 (80%)
 Frame = -2

Query: 722  VYFADLVHMSAQYCNTDKKNPIGLMLLSEVALGDIYEVIKEKEYMDIAPEGKHSTKALGQ 543
            +YFADLV  SAQYC  D+ NP+GLMLLSEVALGD+YE +K+   MD  P GKHSTK LG+
Sbjct: 852  LYFADLVSKSAQYCYVDRNNPVGLMLLSEVALGDMYE-LKKATSMDKPPRGKHSTKGLGK 910

Query: 542  EMPLESEHIKWRGDVTVPCGQPVSSNVEGSEPQCHEYIVYDTAQVKMQFLLKVRFHHKK 366
             +PLESE +KWR DV VPCG+PV S++  SE   +EYIVY+T+QVKMQFLLKVRFHHK+
Sbjct: 911  TVPLESEFVKWRDDVVVPCGKPVPSSIRSSELMYNEYIVYNTSQVKMQFLLKVRFHHKR 969

>dbj|BAC20853.1| putative NAD+ ADP-ribosyltransferase [Oryza sativa (japonica
            cultivar-group)]
          Length = 965

 Score =  164 bits (416), Expect = 9e-40
 Identities = 79/119 (66%), Positives = 97/119 (81%)
 Frame = -2

Query: 722  VYFADLVHMSAQYCNTDKKNPIGLMLLSEVALGDIYEVIKEKEYMDIAPEGKHSTKALGQ 543
            +YFADLV  SAQYC  D+KNP+GLMLLSEVALGD+YE +K+   MD  P GKHSTK LG+
Sbjct: 848  LYFADLVSKSAQYCYVDRKNPVGLMLLSEVALGDMYE-LKKATSMDKPPRGKHSTKGLGK 906

Query: 542  EMPLESEHIKWRGDVTVPCGQPVSSNVEGSEPQCHEYIVYDTAQVKMQFLLKVRFHHKK 366
             +PLESE  KWR DV VPCG+PV ++++ SE   +EYIVY+T+QVKMQ+LLKVRFHHK+
Sbjct: 907  TVPLESEFAKWRDDVVVPCGKPVPASIKTSELMYNEYIVYNTSQVKMQYLLKVRFHHKR 965

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 634,958,282
Number of Sequences: 1393205
Number of extensions: 13907371
Number of successful extensions: 28692
Number of sequences better than 10.0: 84
Number of HSP's better than 10.0 without gapping: 27770
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28601
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 33780557640
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf054b11 BP071376 1 419 2 GNLf004a02 BP074996 15 425 3 GENLf015f08 BP063165 219 724 4 GENLf088a11 BP067125 219 687

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