Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000415A_C01 KMC000415A_c01
(706 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAB16486.1| P0665D10.11 [Oryza sativa (japonica cultivar-gro... 112 2e-40
ref|NP_188117.1| 4-methyl-5(b-hydroxyethyl)-thiazole monophospha... 112 2e-39
dbj|BAA97062.1| emb|CAA17570.1~gene_id:K15M2.13~similar to unkno... 112 2e-39
ref|NP_564626.1| expressed protein; protein id: At1g53280.1, sup... 146 3e-34
gb|AAM60860.1| 4-methyl-5(b-hydroxyethyl)-thiazole monophosphate... 144 2e-33
>dbj|BAB16486.1| P0665D10.11 [Oryza sativa (japonica cultivar-group)]
Length = 517
Score = 112 bits (279), Expect(2) = 2e-40
Identities = 55/86 (63%), Positives = 66/86 (75%)
Frame = -2
Query: 702 IVASRKVKLEADILLDEAAKLSYDLIVLPGGLGGAQAFAKSETLVSLLKNQRESNRYYGA 523
+ K L ADI+++EAAK +DLIV+PGGL GAQ + ++ LV LLK Q ESN+ YGA
Sbjct: 376 VTRRHKFNLIADIMVEEAAKREFDLIVMPGGLPGAQKLSSTKVLVDLLKKQAESNKPYGA 435
Query: 522 ICASPALVLEPHGLLKGKKATAFPAM 445
ICASPA VLEPHGLLKGKKAT+FP M
Sbjct: 436 ICASPAYVLEPHGLLKGKKATSFPPM 461
Score = 76.6 bits (187), Expect(2) = 2e-40
Identities = 37/64 (57%), Positives = 50/64 (77%)
Frame = -3
Query: 470 KRPLLFLPCANKLSDQSEVENRVVVDGNLITSRGPGTSIEFALVIVEKLFGRKLALELAK 291
K+ F P A+ L+DQS ++RVVVDGNLITS+ PG++ EFAL IVEKLFGR+ A+ +AK
Sbjct: 453 KKATSFPPMAHLLTDQSACDSRVVVDGNLITSKAPGSATEFALAIVEKLFGREKAVSIAK 512
Query: 290 TIVF 279
++F
Sbjct: 513 ELIF 516
Score = 65.5 bits (158), Expect(2) = 1e-18
Identities = 35/88 (39%), Positives = 51/88 (57%)
Frame = -2
Query: 702 IVASRKVKLEADILLDEAAKLSYDLIVLPGGLGGAQAFAKSETLVSLLKNQRESNRYYGA 523
+ A+ VKL AD + + ++DLI LPGG+ G+ + L ++K Q E Y A
Sbjct: 170 VEAAFGVKLVADGRVADLEGEAFDLIALPGGMPGSANLRDCKVLEKMVKKQAEQGGLYAA 229
Query: 522 ICASPALVLEPHGLLKGKKATAFPAMCQ 439
ICA+PA+ L GLLKG KAT +P+ +
Sbjct: 230 ICATPAVTLAHWGLLKGLKATCYPSFME 257
Score = 49.7 bits (117), Expect(2) = 1e-18
Identities = 22/41 (53%), Positives = 34/41 (82%)
Frame = -3
Query: 416 VENRVVVDGNLITSRGPGTSIEFALVIVEKLFGRKLALELA 294
V +RVVVD N +TS+GP T+IE+AL +VE+L+G++ + E+A
Sbjct: 266 VNSRVVVDRNAVTSQGPATAIEYALALVEQLYGKEKSEEVA 306
>ref|NP_188117.1| 4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis
protein, putative; protein id: At3g14990.1, supported by
cDNA: gi_11908017, supported by cDNA: gi_13194799,
supported by cDNA: gi_14517477 [Arabidopsis thaliana]
gi|11908018|gb|AAG41438.1|AF326856_1 putative
4-methyl-5(b-hydroxyethyl)-thiazole monophosphate
biosynthesis protein [Arabidopsis thaliana]
gi|13194800|gb|AAK15562.1|AF349515_1 putative
4-methyl-5(b-hydroxyethyl)-thiazole monophosphate
biosynthesis protein [Arabidopsis thaliana]
gi|14517478|gb|AAK62629.1| AT3g14990/K15M2_13
[Arabidopsis thaliana] gi|22136580|gb|AAM91076.1|
AT3g14990/K15M2_13 [Arabidopsis thaliana]
Length = 392
Score = 112 bits (281), Expect(2) = 2e-39
Identities = 55/87 (63%), Positives = 67/87 (76%)
Frame = -2
Query: 705 EIVASRKVKLEADILLDEAAKLSYDLIVLPGGLGGAQAFAKSETLVSLLKNQRESNRYYG 526
E+ SRK KL A++LLDE A+ S+DLIVLPGGL GAQ FA E LV++L+ Q E+N+ YG
Sbjct: 251 EVEGSRKAKLVAEVLLDEVAEKSFDLIVLPGGLNGAQRFASCEKLVNMLRKQAEANKPYG 310
Query: 525 AICASPALVLEPHGLLKGKKATAFPAM 445
ICASPA V EP+GLLKGKKAT P +
Sbjct: 311 GICASPAYVFEPNGLLKGKKATTHPVV 337
Score = 72.0 bits (175), Expect(2) = 3e-22
Identities = 36/86 (41%), Positives = 50/86 (57%)
Frame = -2
Query: 696 ASRKVKLEADILLDEAAKLSYDLIVLPGGLGGAQAFAKSETLVSLLKNQRESNRYYGAIC 517
A +K+ AD LL + +DLIVLPGGL G + ++L +++K Q R AIC
Sbjct: 48 ACHGIKMVADTLLSDITDSVFDLIVLPGGLPGGETLKNCKSLENMVKKQDSDGRLNAAIC 107
Query: 516 ASPALVLEPHGLLKGKKATAFPAMCQ 439
+PAL L GLL+GKKAT +P +
Sbjct: 108 CAPALALGTWGLLEGKKATGYPVFME 133
Score = 72.0 bits (175), Expect(2) = 2e-39
Identities = 33/51 (64%), Positives = 45/51 (87%)
Frame = -3
Query: 443 ANKLSDQSEVENRVVVDGNLITSRGPGTSIEFALVIVEKLFGRKLALELAK 291
++KLSD+S +E+RVVVDGN+ITSR PGT++EF+L IVEK +GR+ AL+L K
Sbjct: 338 SDKLSDKSHIEHRVVVDGNVITSRAPGTAMEFSLAIVEKFYGREKALQLGK 388
Score = 55.1 bits (131), Expect(2) = 3e-22
Identities = 22/45 (48%), Positives = 38/45 (83%)
Frame = -3
Query: 416 VENRVVVDGNLITSRGPGTSIEFALVIVEKLFGRKLALELAKTIV 282
VE+RV +DG ++TSRGPGT+IEF++ ++E+LFG++ A E++ ++
Sbjct: 143 VESRVQIDGRIVTSRGPGTTIEFSITLIEQLFGKEKADEVSSILL 187
>dbj|BAA97062.1| emb|CAA17570.1~gene_id:K15M2.13~similar to unknown protein
[Arabidopsis thaliana]
Length = 369
Score = 112 bits (281), Expect(2) = 2e-39
Identities = 55/87 (63%), Positives = 67/87 (76%)
Frame = -2
Query: 705 EIVASRKVKLEADILLDEAAKLSYDLIVLPGGLGGAQAFAKSETLVSLLKNQRESNRYYG 526
E+ SRK KL A++LLDE A+ S+DLIVLPGGL GAQ FA E LV++L+ Q E+N+ YG
Sbjct: 228 EVEGSRKAKLVAEVLLDEVAEKSFDLIVLPGGLNGAQRFASCEKLVNMLRKQAEANKPYG 287
Query: 525 AICASPALVLEPHGLLKGKKATAFPAM 445
ICASPA V EP+GLLKGKKAT P +
Sbjct: 288 GICASPAYVFEPNGLLKGKKATTHPVV 314
Score = 72.0 bits (175), Expect(2) = 3e-22
Identities = 36/86 (41%), Positives = 50/86 (57%)
Frame = -2
Query: 696 ASRKVKLEADILLDEAAKLSYDLIVLPGGLGGAQAFAKSETLVSLLKNQRESNRYYGAIC 517
A +K+ AD LL + +DLIVLPGGL G + ++L +++K Q R AIC
Sbjct: 25 ACHGIKMVADTLLSDITDSVFDLIVLPGGLPGGETLKNCKSLENMVKKQDSDGRLNAAIC 84
Query: 516 ASPALVLEPHGLLKGKKATAFPAMCQ 439
+PAL L GLL+GKKAT +P +
Sbjct: 85 CAPALALGTWGLLEGKKATGYPVFME 110
Score = 72.0 bits (175), Expect(2) = 2e-39
Identities = 33/51 (64%), Positives = 45/51 (87%)
Frame = -3
Query: 443 ANKLSDQSEVENRVVVDGNLITSRGPGTSIEFALVIVEKLFGRKLALELAK 291
++KLSD+S +E+RVVVDGN+ITSR PGT++EF+L IVEK +GR+ AL+L K
Sbjct: 315 SDKLSDKSHIEHRVVVDGNVITSRAPGTAMEFSLAIVEKFYGREKALQLGK 365
Score = 55.1 bits (131), Expect(2) = 3e-22
Identities = 22/45 (48%), Positives = 38/45 (83%)
Frame = -3
Query: 416 VENRVVVDGNLITSRGPGTSIEFALVIVEKLFGRKLALELAKTIV 282
VE+RV +DG ++TSRGPGT+IEF++ ++E+LFG++ A E++ ++
Sbjct: 120 VESRVQIDGRIVTSRGPGTTIEFSITLIEQLFGKEKADEVSSILL 164
>ref|NP_564626.1| expressed protein; protein id: At1g53280.1, supported by cDNA:
101735., supported by cDNA: gi_20259560 [Arabidopsis
thaliana] gi|7769869|gb|AAF69547.1|AC008007_22 F12M16.18
[Arabidopsis thaliana] gi|15810459|gb|AAL07117.1|
unknown protein [Arabidopsis thaliana]
gi|20259561|gb|AAM14123.1| unknown protein [Arabidopsis
thaliana]
Length = 438
Score = 146 bits (368), Expect = 3e-34
Identities = 73/88 (82%), Positives = 79/88 (88%)
Frame = -2
Query: 705 EIVASRKVKLEADILLDEAAKLSYDLIVLPGGLGGAQAFAKSETLVSLLKNQRESNRYYG 526
E+VASRKVKL AD+LLDEA K SYDLIVLPGGLGGA+AFA SE LV++LK Q ESN+ YG
Sbjct: 297 EVVASRKVKLVADVLLDEAEKNSYDLIVLPGGLGGAEAFASSEKLVNMLKKQAESNKPYG 356
Query: 525 AICASPALVLEPHGLLKGKKATAFPAMC 442
AICASPALV EPHGLLKGKKATAFPAMC
Sbjct: 357 AICASPALVFEPHGLLKGKKATAFPAMC 384
Score = 77.8 bits (190), Expect = 1e-13
Identities = 37/60 (61%), Positives = 48/60 (79%)
Frame = -3
Query: 470 KRPLLFLPCANKLSDQSEVENRVVVDGNLITSRGPGTSIEFALVIVEKLFGRKLALELAK 291
K+ F +KL+DQS +E+RV+VDGNLITSRGPGTS+EFAL IVEK +GR+ L+L+K
Sbjct: 375 KKATAFPAMCSKLTDQSHIEHRVLVDGNLITSRGPGTSLEFALAIVEKFYGREKGLQLSK 434
Score = 67.8 bits (164), Expect(2) = 1e-20
Identities = 34/86 (39%), Positives = 47/86 (54%)
Frame = -2
Query: 696 ASRKVKLEADILLDEAAKLSYDLIVLPGGLGGAQAFAKSETLVSLLKNQRESNRYYGAIC 517
A +K+ AD LL + +DLI+LPGGL G + + L ++K Q R AIC
Sbjct: 95 ACHGIKMVADTLLSDITDSVFDLIMLPGGLPGGETLKNCKPLEKMVKKQDTDGRLNAAIC 154
Query: 516 ASPALVLEPHGLLKGKKATAFPAMCQ 439
+PAL GLL+GKKAT +P +
Sbjct: 155 CAPALAFGTWGLLEGKKATCYPVFME 180
Score = 53.9 bits (128), Expect(2) = 1e-20
Identities = 22/45 (48%), Positives = 38/45 (83%)
Frame = -3
Query: 416 VENRVVVDGNLITSRGPGTSIEFALVIVEKLFGRKLALELAKTIV 282
VE+RV +DG ++TSRGPGT++EF++ +VE+L G++ A+E++ +V
Sbjct: 189 VESRVEIDGKIVTSRGPGTTMEFSVTLVEQLLGKEKAVEVSGPLV 233
>gb|AAM60860.1| 4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis
protein, putative [Arabidopsis thaliana]
Length = 438
Score = 144 bits (362), Expect = 2e-33
Identities = 72/88 (81%), Positives = 78/88 (87%)
Frame = -2
Query: 705 EIVASRKVKLEADILLDEAAKLSYDLIVLPGGLGGAQAFAKSETLVSLLKNQRESNRYYG 526
E+VASRKVKL AD+LLDEA K YDLIVLPGGLGGA+AFA SE LV++LK Q ESN+ YG
Sbjct: 297 EVVASRKVKLVADVLLDEAEKNLYDLIVLPGGLGGAEAFASSEKLVNMLKKQAESNKPYG 356
Query: 525 AICASPALVLEPHGLLKGKKATAFPAMC 442
AICASPALV EPHGLLKGKKATAFPAMC
Sbjct: 357 AICASPALVFEPHGLLKGKKATAFPAMC 384
Score = 77.8 bits (190), Expect = 1e-13
Identities = 37/60 (61%), Positives = 48/60 (79%)
Frame = -3
Query: 470 KRPLLFLPCANKLSDQSEVENRVVVDGNLITSRGPGTSIEFALVIVEKLFGRKLALELAK 291
K+ F +KL+DQS +E+RV+VDGNLITSRGPGTS+EFAL IVEK +GR+ L+L+K
Sbjct: 375 KKATAFPAMCSKLTDQSHIEHRVLVDGNLITSRGPGTSLEFALAIVEKFYGREKGLQLSK 434
Score = 67.4 bits (163), Expect(2) = 2e-20
Identities = 34/86 (39%), Positives = 47/86 (54%)
Frame = -2
Query: 696 ASRKVKLEADILLDEAAKLSYDLIVLPGGLGGAQAFAKSETLVSLLKNQRESNRYYGAIC 517
A +K+ AD LL + +DLI+LPGGL G + + L ++K Q R AIC
Sbjct: 95 ACHGIKMVADTLLSDITDSVFDLIMLPGGLPGGETLKNCKPLERMVKKQDTDGRLNAAIC 154
Query: 516 ASPALVLEPHGLLKGKKATAFPAMCQ 439
+PAL GLL+GKKAT +P +
Sbjct: 155 CAPALAFGTWGLLEGKKATCYPVFME 180
Score = 53.9 bits (128), Expect(2) = 2e-20
Identities = 22/45 (48%), Positives = 38/45 (83%)
Frame = -3
Query: 416 VENRVVVDGNLITSRGPGTSIEFALVIVEKLFGRKLALELAKTIV 282
VE+RV +DG ++TSRGPGT++EF++ +VE+L G++ A+E++ +V
Sbjct: 189 VESRVEIDGKIVTSRGPGTTMEFSVTLVEQLLGKEKAVEVSGPLV 233
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 598,716,221
Number of Sequences: 1393205
Number of extensions: 12390218
Number of successful extensions: 30618
Number of sequences better than 10.0: 171
Number of HSP's better than 10.0 without gapping: 29548
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30580
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 32091529758
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
