>KMC000397A_C01 KMC000397A_c01
aAAAAGCGAAATGGATTTTATGTGTATCCAATGAATGTAAAGGTAACTTCGCATAATACA
AAACATATATTTGGTAAGTTGAAGATACAAAACAAGCTTTGACAAAAAAAAAAGCAAGCT
AGAGAAATGCACTAAGCACAAACTAAGCTAGAAAGAAAAAAGGATGGCCGTACACATGAC
ACTAATCCATGAATTTGGAATATTTTAGAACTTTTTTTTTGGACAATGTAATATTTTAGA
ACTTATGTAACCAATAAAAATGCATGAAAAGACTAAAACATGATTTGTCATCTGCTTTTC
CCTTTGCGCTTAGATCTCCTACTTGGCTTGCCCTTCTTGAAGCTCTTAGAAACTATGTTG
CTACCATTTCCAACCCCATCAAGACTAGCCTTGACCTCAAGGCGGGCTCGGGCGAAGTTG
GATGGATGGATGGCTCAAGATGGGACCTGCTGGCGAGTGCGAAGAAGTGGGCTGGTGATC
TGAAACTCCACAATCTAAAGGTGAAGAAAGAACTGTTGGTACTCGGTTTGATGGTTGCGA
TGTGGCTTCAATGGTACCCACAGATAAAAATCTACGAAATAAGTATAGATGTTCAAACTT
ATCTGATCACTGAAGTCTGAAGCC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000397A_C01 KMC000397A_c01
         (624 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_705264.1| hypothetical protein [Plasmodium falciparum 3D7...    35  0.61
ref|NP_198672.1| putative protein; protein id: At5g38560.1, supp...    34  1.8
ref|NP_031729.1| cold inducible glycoprotein 30 [Mus musculus] g...    33  4.0
ref|XP_192861.1| elongation of very long chain fatty acids (FEN1...    33  4.0
gb|ZP_00017631.1| hypothetical protein [Chloroflexus aurantiacus]      32  6.7

>ref|NP_705264.1| hypothetical protein [Plasmodium falciparum 3D7]
           gi|23615509|emb|CAD52501.1| hypothetical protein
           [Plasmodium falciparum 3D7]
          Length = 324

 Score = 35.4 bits (80), Expect = 0.61
 Identities = 25/78 (32%), Positives = 37/78 (47%), Gaps = 2/78 (2%)
 Frame = -3

Query: 280 CFSLFMH-FYWLHKF*NITLSKKKVLKYSKFMD*CHVYGHPFFFLA*FVL-SAFL*LAFF 107
           CF+++   FYWLH + N TLSK+  LKY        +Y +  F  + F     F  L  F
Sbjct: 244 CFNIYNTLFYWLHIYLNFTLSKRIYLKYKT------LYKNISFVFSYFSFDEIFFFLIIF 297

Query: 106 FVKACFVSSTYQIYVLYY 53
           +    F S+   + + YY
Sbjct: 298 YGFFSFTSTILTLTLFYY 315

>ref|NP_198672.1| putative protein; protein id: At5g38560.1, supported by cDNA:
           gi_15983496, supported by cDNA: gi_18700152 [Arabidopsis
           thaliana] gi|10176824|dbj|BAB10146.1| contains
           similarity to protein kinase~gene_id:MBB18.10
           [Arabidopsis thaliana]
           gi|15983497|gb|AAL11616.1|AF424623_1 AT5g38560/MBB18_10
           [Arabidopsis thaliana] gi|18700153|gb|AAL77688.1|
           AT5g38560/MBB18_10 [Arabidopsis thaliana]
           gi|21360463|gb|AAM47347.1| AT5g38560/MBB18_10
           [Arabidopsis thaliana]
          Length = 681

 Score = 33.9 bits (76), Expect = 1.8
 Identities = 18/47 (38%), Positives = 25/47 (52%)
 Frame = -1

Query: 540 SQPSNRVPTVLSSPLDCGVSDHQPTSSHSPAGPILSHPSIQLRPSPP 400
           S P +  P V SSP    VS   P+SS  P+ P+++ P   +  SPP
Sbjct: 43  SPPQSPPPVVSSSPPPPVVSSPPPSSSPPPSPPVITSPPPTVASSPP 89

>ref|NP_031729.1| cold inducible glycoprotein 30 [Mus musculus]
           gi|20137691|sp|O35949|ELO3_MOUSE Elongation of very long
           chain fatty acids protein 3 (Cold inducible glycoprotein
           of 30 kDa) (CIN-2) gi|2289244|gb|AAC06127.1| membrane
           glycoprotein CIG30 [Mus musculus]
           gi|5762267|gb|AAD51088.1|AF054504_1 membrane
           glycoprotein [Mus musculus] gi|16741263|gb|AAH16468.1|
           elongation of very long chain fatty acids (FEN1/Elo2,
           SUR4/Elo3, yeast)-like 3 [Mus musculus]
          Length = 271

 Score = 32.7 bits (73), Expect = 4.0
 Identities = 17/45 (37%), Positives = 26/45 (57%)
 Frame = -1

Query: 135 LVHFSSLLFFLSKLVLYLQLTKYMFCIMRSYLYIHWIHIKSISLF 1
           +V F S LF LSK+V  L  T ++    R  +++HW H  ++ LF
Sbjct: 113 VVRFWSFLFLLSKVV-ELGDTAFIILRKRPLIFVHWYHHSTVLLF 156

>ref|XP_192861.1| elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3,
           yeast)-like 3 [Mus musculus]
          Length = 216

 Score = 32.7 bits (73), Expect = 4.0
 Identities = 17/45 (37%), Positives = 26/45 (57%)
 Frame = -1

Query: 135 LVHFSSLLFFLSKLVLYLQLTKYMFCIMRSYLYIHWIHIKSISLF 1
           +V F S LF LSK+V  L  T ++    R  +++HW H  ++ LF
Sbjct: 58  VVRFWSFLFLLSKVV-ELGDTAFIILRKRPLIFVHWYHHSTVLLF 101

>gb|ZP_00017631.1| hypothetical protein [Chloroflexus aurantiacus]
          Length = 288

 Score = 32.0 bits (71), Expect = 6.7
 Identities = 20/52 (38%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
 Frame = -1

Query: 543 TSQPSNRVPTVLSSPLDCGVSDHQPTSSHSPA-GPILSHPSIQLRPSPP*GQ 391
           T+QP+  +PT L SP     +D   TSS SPA  P ++ P     P+   GQ
Sbjct: 212 TAQPATAIPTALPSPTAIRQADTINTSSPSPAVTPAMTPPPAGSAPASSGGQ 263

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 536,656,091
Number of Sequences: 1393205
Number of extensions: 11806861
Number of successful extensions: 36565
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 34634
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36459
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25301904073
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENLf015a01 BP063120 1 490 2 SPDL028b06_f BP053716 2 509 3 GENLf076d03 BP066466 41 533 4 SPDL045f11_f BP054838 51 261 5 MFBL045g12_f BP043569 196 628

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