Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000392A_C01 KMC000392A_c01
(641 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C96789 protein T23E18.6 [imported] - Arabidopsis thaliana g... 132 5e-30
ref|NP_177740.1| alpha-amylase, putative; protein id: At1g76130.... 132 5e-30
gb|AAF63239.1|AF153828_1 alpha-amylase [Malus x domestica] 127 1e-28
emb|CAD40677.1| OSJNBb0118P14.15 [Oryza sativa (japonica cultiva... 126 3e-28
gb|AAA32925.1| alpha-amylase 1 123 2e-27
>pir||C96789 protein T23E18.6 [imported] - Arabidopsis thaliana
gi|6573706|gb|AAF17626.1|AC009978_2 T23E18.6
[Arabidopsis thaliana]
Length = 412
Score = 132 bits (331), Expect = 5e-30
Identities = 63/121 (52%), Positives = 78/121 (64%), Gaps = 2/121 (1%)
Frame = -2
Query: 640 ENHDTGSTQXHWPFPRDKLMQGYAYILTHPGTPVIFYDHFYDFG--IHDVITELIEARRR 467
+NHDTGSTQ HWPFP +M+GYAYILTHPG P +FYDHFYD+G IHD I +LI+ RRR
Sbjct: 296 DNHDTGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRR 355
Query: 466 AGIHCRSSIKIIHAHNEGYVAQVGDALVMKLGHFDWNPSKENQLEGSWQKFVDKGQDYQV 287
IH RS+++++ A + Y A VG+ + MKLG W PS G G Y V
Sbjct: 356 QDIHSRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPS------GRDWTLATSGHRYAV 409
Query: 286 W 284
W
Sbjct: 410 W 410
>ref|NP_177740.1| alpha-amylase, putative; protein id: At1g76130.1, supported by
cDNA: 119931. [Arabidopsis thaliana]
gi|21537093|gb|AAM61434.1| alpha-amylase, putative
[Arabidopsis thaliana]
Length = 413
Score = 132 bits (331), Expect = 5e-30
Identities = 63/121 (52%), Positives = 78/121 (64%), Gaps = 2/121 (1%)
Frame = -2
Query: 640 ENHDTGSTQXHWPFPRDKLMQGYAYILTHPGTPVIFYDHFYDFG--IHDVITELIEARRR 467
+NHDTGSTQ HWPFP +M+GYAYILTHPG P +FYDHFYD+G IHD I +LI+ RRR
Sbjct: 297 DNHDTGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRR 356
Query: 466 AGIHCRSSIKIIHAHNEGYVAQVGDALVMKLGHFDWNPSKENQLEGSWQKFVDKGQDYQV 287
IH RS+++++ A + Y A VG+ + MKLG W PS G G Y V
Sbjct: 357 QDIHSRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPS------GRDWTLATSGHRYAV 410
Query: 286 W 284
W
Sbjct: 411 W 411
>gb|AAF63239.1|AF153828_1 alpha-amylase [Malus x domestica]
Length = 413
Score = 127 bits (320), Expect = 1e-28
Identities = 61/121 (50%), Positives = 76/121 (62%), Gaps = 2/121 (1%)
Frame = -2
Query: 640 ENHDTGSTQXHWPFPRDKLMQGYAYILTHPGTPVIFYDHFYDFG--IHDVITELIEARRR 467
+NHDTGSTQ HWPFP + +M+GY YILTHPG P +FYDHFYD+G IHD I +LI+ R+R
Sbjct: 297 DNHDTGSTQAHWPFPTNHIMEGYTYILTHPGIPTVFYDHFYDWGDSIHDQIVKLIDIRKR 356
Query: 466 AGIHCRSSIKIIHAHNEGYVAQVGDALVMKLGHFDWNPSKENQLEGSWQKFVDKGQDYQV 287
IH RSSI I+ A Y A +G+ + MK+G W P+ G G Y V
Sbjct: 357 QDIHSRSSITILEAQPNLYSAMIGEKVCMKIGDGSWCPA------GREWTLATCGHRYAV 410
Query: 286 W 284
W
Sbjct: 411 W 411
>emb|CAD40677.1| OSJNBb0118P14.15 [Oryza sativa (japonica cultivar-group)]
Length = 412
Score = 126 bits (316), Expect = 3e-28
Identities = 63/121 (52%), Positives = 75/121 (61%), Gaps = 2/121 (1%)
Frame = -2
Query: 640 ENHDTGSTQXHWPFPRDKLMQGYAYILTHPGTPVIFYDHFY--DFGIHDVITELIEARRR 467
ENHDTGSTQ HWPFP D +M+GYAYILTHPG P +FYDHFY D H I +L+E R+
Sbjct: 296 ENHDTGSTQGHWPFPSDHIMEGYAYILTHPGIPTVFYDHFYGKDDSFHGGIAKLMEIRKC 355
Query: 466 AGIHCRSSIKIIHAHNEGYVAQVGDALVMKLGHFDWNPSKENQLEGSWQKFVDKGQDYQV 287
IH RS++KI+ A ++ Y A V D L MK+G W PS G K G Y V
Sbjct: 356 QDIHSRSAVKILEASSDLYSAIVDDKLCMKIGDGSWCPS------GPEWKLAASGDRYAV 409
Query: 286 W 284
W
Sbjct: 410 W 410
>gb|AAA32925.1| alpha-amylase 1
Length = 427
Score = 123 bits (309), Expect = 2e-27
Identities = 60/120 (50%), Positives = 80/120 (66%), Gaps = 1/120 (0%)
Frame = -2
Query: 640 ENHDTGSTQXHWPFPRDKLMQGYAYILTHPGTPVIFYDHFYDFGIHDVITELIEARRRAG 461
+NHDTGSTQ WPFP D++MQGYAYILTHPGTP IFYDHF+D+G+ + I L+ R R G
Sbjct: 310 DNHDTGSTQHMWPFPSDRVMQGYAYILTHPGTPCIFYDHFFDWGLKEEIDRLVSVRTRHG 369
Query: 460 IHCRSSIKIIHAHNEGYVAQVGDALVMKLG-HFDWNPSKENQLEGSWQKFVDKGQDYQVW 284
IH S ++II A + Y+A++ +++KLG +D N + G + K G DY VW
Sbjct: 370 IHNESKLQIIEADADLYLAEIDGKVIVKLGPRYDVG----NLIPGGF-KVAAHGNDYAVW 424
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 561,691,477
Number of Sequences: 1393205
Number of extensions: 12204907
Number of successful extensions: 36102
Number of sequences better than 10.0: 85
Number of HSP's better than 10.0 without gapping: 35322
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36082
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 27007650415
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
