Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000351A_C01 KMC000351A_c01
(827 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_565330.1| subtilisin-like serine protease, putative; prot... 111 5e-43
gb|AAM22744.1|AC092388_28 putative cucumisin-like serine proteas... 92 1e-28
pir||T07171 subtilisin-like proteinase (EC 3.4.21.-) 1 - tomato ... 61 6e-17
pir||T07172 subtilisin-like proteinase (EC 3.4.21.-) 2 - tomato ... 64 2e-16
ref|NP_569048.1| subtilisin-like serine protease ARA12; protein ... 57 5e-16
>ref|NP_565330.1| subtilisin-like serine protease, putative; protein id: At2g05920.1,
supported by cDNA: gi_14334833 [Arabidopsis thaliana]
gi|25289829|pir||A84473 probable serine proteinase
[imported] - Arabidopsis thaliana
gi|4006827|gb|AAC95169.1| subtilisin-like serine
protease, putative [Arabidopsis thaliana]
gi|14334834|gb|AAK59595.1| putative subtilisin serine
protease [Arabidopsis thaliana]
gi|23296838|gb|AAN13182.1| putative subtilisin serine
protease [Arabidopsis thaliana]
Length = 754
Score = 111 bits (277), Expect(2) = 5e-43
Identities = 55/87 (63%), Positives = 64/87 (73%)
Frame = -1
Query: 827 LVYDAKARDYLAFLCSLDYSPDHLQLIVKRAGVNCSRKLSDPGQLHYPSFSVVFDSVVFD 648
LVYD +Y+ FLCSLDY+ DH+ IVKR VNCS+K SDPGQL+YPSF SV+F
Sbjct: 608 LVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF-----SVLFG 662
Query: 647 AKKVVRYTRTVTNVGEAGSVYDVVVDG 567
K+VVRYTR VTNVG A SVY V V+G
Sbjct: 663 GKRVVRYTREVTNVGAASSVYKVTVNG 689
Score = 86.3 bits (212), Expect(2) = 5e-43
Identities = 42/68 (61%), Positives = 53/68 (77%)
Frame = -2
Query: 580 LSLMGLPWLGITVYPTKLEFGKVGERKRYTVTFVSKKGASDDLVRSAFGSITWKNEQHQV 401
+++ G P +GI+V P+KL F VGE+KRYTVTFVSKKG S ++ FGSITW N QH+V
Sbjct: 685 VTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVS-MTNKAEFGSITWSNPQHEV 743
Query: 400 RSPVAFAW 377
RSPVAF+W
Sbjct: 744 RSPVAFSW 751
>gb|AAM22744.1|AC092388_28 putative cucumisin-like serine protease [Oryza sativa (japonica
cultivar-group)]
Length = 773
Score = 92.0 bits (227), Expect(2) = 1e-28
Identities = 46/89 (51%), Positives = 62/89 (68%)
Frame = -1
Query: 827 LVYDAKARDYLAFLCSLDYSPDHLQLIVKRAGVNCSRKLSDPGQLHYPSFSVVFDSVVFD 648
L+YD +DY++FLCSL+Y+ H+Q+I K + + C RK PG L+YPSFSVVF
Sbjct: 628 LLYDISTKDYVSFLCSLNYTTPHIQVITKMSNITCPRKFR-PGDLNYPSFSVVFKK---K 683
Query: 647 AKKVVRYTRTVTNVGEAGSVYDVVVDGPS 561
+K V+R+ R VTNVG A SVY+V V GP+
Sbjct: 684 SKHVMRFRREVTNVGPAMSVYNVKVSGPA 712
Score = 57.0 bits (136), Expect(2) = 1e-28
Identities = 27/60 (45%), Positives = 38/60 (63%)
Frame = -2
Query: 556 LGITVYPTKLEFGKVGERKRYTVTFVSKKGASDDLVRSAFGSITWKNEQHQVRSPVAFAW 377
+ + V P KL F KVG+++RY V F S AS+ + FG I+W + QH VRSP+A+ W
Sbjct: 714 VSVKVTPAKLVFNKVGQKQRYYVIFASTVDASN--AKPDFGWISWMSSQHVVRSPIAYTW 771
>pir||T07171 subtilisin-like proteinase (EC 3.4.21.-) 1 - tomato
gi|1771160|emb|CAA67429.1| SBT1 [Lycopersicon
esculentum] gi|3687305|emb|CAA06999.1| subtilisin-like
protease [Lycopersicon esculentum]
Length = 766
Score = 60.8 bits (146), Expect(2) = 6e-17
Identities = 35/81 (43%), Positives = 48/81 (59%), Gaps = 6/81 (7%)
Frame = -1
Query: 827 LVYDAKARDYLAFLCSLDYSPDHLQLIVKRAGVNC-SRKLSDPGQLHYPSFSVVFDSVVF 651
LVYD DY+ FLC+LDYSP +++I KR ++C K L+YPSFS+ ++
Sbjct: 614 LVYDLTVDDYINFLCALDYSPSMIKVIAKR-DISCDENKEYRVADLNYPSFSIPMETAWG 672
Query: 650 D-----AKKVVRYTRTVTNVG 603
+ V RYTRT+TNVG
Sbjct: 673 EHADSSTPTVTRYTRTLTNVG 693
Score = 48.9 bits (115), Expect(2) = 6e-17
Identities = 24/59 (40%), Positives = 33/59 (55%)
Frame = -2
Query: 550 ITVYPTKLEFGKVGERKRYTVTFVSKKGASDDLVRSAFGSITWKNEQHQVRSPVAFAWT 374
I V P L F + E+K YTVTF + S ++F + W + QH V SP+AF+WT
Sbjct: 711 ILVEPQTLTFSRKNEKKTYTVTFTATSKPSGT---TSFARLEWSDGQHVVASPIAFSWT 766
>pir||T07172 subtilisin-like proteinase (EC 3.4.21.-) 2 - tomato
gi|1771162|emb|CAA67430.1| SBT2 [Lycopersicon
esculentum] gi|3687307|emb|CAA07000.1| subtilisin-like
protease [Lycopersicon esculentum]
Length = 775
Score = 64.3 bits (155), Expect(2) = 2e-16
Identities = 35/85 (41%), Positives = 47/85 (55%)
Frame = -1
Query: 827 LVYDAKARDYLAFLCSLDYSPDHLQLIVKRAGVNCSRKLSDPGQLHYPSFSVVFDSVVFD 648
L+YD A+DY FLC+ + SP L + K + C L++PG L+YP+ S VF
Sbjct: 628 LIYDIGAQDYFEFLCTQELSPSQLMVFGKFSNRTCHHSLANPGDLNYPAISAVFPEKT-- 685
Query: 647 AKKVVRYTRTVTNVGEAGSVYDVVV 573
++ RTVTNVG S Y VVV
Sbjct: 686 KLSMLTLHRTVTNVGSPISNYHVVV 710
Score = 43.9 bits (102), Expect(2) = 2e-16
Identities = 23/60 (38%), Positives = 34/60 (56%), Gaps = 2/60 (3%)
Frame = -2
Query: 550 ITVYPTKLEFGKVGERKRYTVTF--VSKKGASDDLVRSAFGSITWKNEQHQVRSPVAFAW 377
+ V P +L F ++ Y VTF VS++ A + FGS+ WK+ H+VRSP+A W
Sbjct: 718 VKVEPERLNFTSKNQKLSYKVTFKTVSRQKAPE------FGSLIWKDGTHKVRSPIAITW 771
>ref|NP_569048.1| subtilisin-like serine protease ARA12; protein id: At5g67360.1,
supported by cDNA: gi_13430745, supported by cDNA:
gi_20147210 [Arabidopsis thaliana]
gi|25289825|pir||JC7519 subtilisin-like serine
proteinase (EC 3.4.21.-) - Arabidopsis thaliana
gi|3176874|gb|AAC18851.1| cucumisin-like serine protease
[Arabidopsis thaliana] gi|9758435|dbj|BAB09021.1|
cucumisin-like serine protease [Arabidopsis thaliana]
gi|13430746|gb|AAK25995.1|AF360285_1 putative subtilisin
serine protease ARA12 [Arabidopsis thaliana]
gi|23296832|gb|AAN13181.1| putative subtilisin serine
protease ARA12 [Arabidopsis thaliana]
Length = 757
Score = 57.0 bits (136), Expect(2) = 5e-16
Identities = 34/85 (40%), Positives = 47/85 (55%)
Frame = -1
Query: 827 LVYDAKARDYLAFLCSLDYSPDHLQLIVKRAGVNCSRKLSDPGQLHYPSFSVVFDSVVFD 648
L+YD DYL FLC+L+Y+ ++ + +R K L+YPSF+V D V
Sbjct: 614 LIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV--- 670
Query: 647 AKKVVRYTRTVTNVGEAGSVYDVVV 573
+YTRTVT+VG AG+ Y V V
Sbjct: 671 --GAYKYTRTVTSVGGAGT-YSVKV 692
Score = 49.7 bits (117), Expect(2) = 5e-16
Identities = 28/62 (45%), Positives = 36/62 (57%), Gaps = 3/62 (4%)
Frame = -2
Query: 550 ITVYPTKLEFGKVGERKRYTVTFV---SKKGASDDLVRSAFGSITWKNEQHQVRSPVAFA 380
I+V P L F + E+K YTVTF SK S+ +FGSI W + +H V SPVA +
Sbjct: 701 ISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN-----SFGSIEWSDGKHVVGSPVAIS 755
Query: 379 WT 374
WT
Sbjct: 756 WT 757
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 729,982,223
Number of Sequences: 1393205
Number of extensions: 16907822
Number of successful extensions: 59931
Number of sequences better than 10.0: 192
Number of HSP's better than 10.0 without gapping: 54009
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 59317
length of database: 448,689,247
effective HSP length: 122
effective length of database: 278,718,237
effective search space used: 42643890261
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
