>KMC000327A_C01 KMC000327A_c01
acgttaaaaattgcaatggaagcgtttcatttaagccaatgaaaaaaccaaaaagtaatg
atttcatgactgcaTAAACAGGATATGAACAATTATCCAGCAGTGGTATTGTGACTCAGG
TAAACTGGAGAGGGCCACTTTATTATAAATTAAACAGGATATAAACAATTATCCACCAGG
GGTATTGTGACTCAGGTAAACTTTATCTGCTATGTAGCTTGAATCCTGGAGTTGCCATGG
TGTGGAACAAAAACAGGATATAACAAATTGATCTCTTAAACTCCGTTTTTTTTTTTTCTA
CTAGACAAGTTGTATATGATCAAGGGGGCGTGCGGTCCAGCTCCAAAAATATTTATAGCA
TATTCAGTTATGTGCTTGGAAGTCTCATAATCACTTTGGGAAAAAAAATTCTCAGCTTTA
GCTTTGGGAGTTAACATGCTTTGAATTGTGTGTCCAAGGTCAATAATCCAAACACGCGAA
GGGTCAAGGAAAGCCTTGGGTAATGGTTCAACTCCCCCCAAACTTTCAGCTCAACTTCTT
CCCATGATTCTGCTGATCTCAGTTCTTCCCATATGCTAAAAATAGCTCCCAAGACATGGT
CATCATGCAAAATACAATAGCACATGGCAATATATGAGAGTCGGCTAAACTTTGGACCAC
CATCTGTGGACTCGGAATTCCCTATGTTACTAGGAAAAGGAAGTTGGTAACTTTCTAAAA
ATCGCTTCAGGAGATTGGAATCACCTCTAAACACATCTAAATAAGGTGGTATCAAGTCAA
GAATAGGATCACCTATAGAAAGATCACTGAAATCAAGAAT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000327A_C01 KMC000327A_c01
         (820 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_177951.1| unknown protein; protein id: At1g78280.1 [Arabi...   132  5e-30
pir||A96812 protein F3F9.18 [imported] - Arabidopsis thaliana gi...   118  9e-26
sp|P09979|KHYB_STRHY HYGROMYCIN-B KINASE (HYGROMYCIN B PHOSPHOTR...    33  5.1
ref|XP_222567.1| similar to heat shock protein 86 [Rattus norveg...    33  6.6

>ref|NP_177951.1| unknown protein; protein id: At1g78280.1 [Arabidopsis thaliana]
          Length = 889

 Score =  132 bits (333), Expect = 5e-30
 Identities = 68/112 (60%), Positives = 83/112 (73%), Gaps = 5/112 (4%)
 Frame = -1

Query: 820  ILDFSDLSIGDPILDLIPPYLDVFRGDSNLLKRFLESYQLPF----PSNIGNSESTDGG- 656
            ILDFSDL+IGDPI DLIP YLDVFRGD++LLK+ LE+Y LP      S  G +++ D   
Sbjct: 778  ILDFSDLTIGDPICDLIPIYLDVFRGDADLLKKLLENYGLPLIRSRSSENGTTKTADSTR 837

Query: 655  PKFSRLSYIAMCYCILHDDHVLGAIFSIWEELRSAESWEEVELKVWGELNHY 500
             K    SY  MCYCILH+++VLG+IFSIW+ELR+AESWE+VE  VW  LN Y
Sbjct: 838  KKVLSPSYRTMCYCILHEENVLGSIFSIWDELRTAESWEQVEQTVWSLLNTY 889

>pir||A96812 protein F3F9.18 [imported] - Arabidopsis thaliana
            gi|8052543|gb|AAF71807.1|AC013430_16 F3F9.18 [Arabidopsis
            thaliana]
          Length = 919

 Score =  118 bits (296), Expect = 9e-26
 Identities = 68/138 (49%), Positives = 83/138 (59%), Gaps = 31/138 (22%)
 Frame = -1

Query: 820  ILDFSDLSIG--------------------------DPILDLIPPYLDVFRGDSNLLKRF 719
            ILDFSDL+IG                          DPI DLIP YLDVFRGD++LLK+ 
Sbjct: 782  ILDFSDLTIGKSYLRDISQIHNPNAEISERVEEIAGDPICDLIPIYLDVFRGDADLLKKL 841

Query: 718  LESYQLPF----PSNIGNSESTDGG-PKFSRLSYIAMCYCILHDDHVLGAIFSIWEELRS 554
            LE+Y LP      S  G +++ D    K    SY  MCYCILH+++VLG+IFSIW+ELR+
Sbjct: 842  LENYGLPLIRSRSSENGTTKTADSTRKKVLSPSYRTMCYCILHEENVLGSIFSIWDELRT 901

Query: 553  AESWEEVELKVWGELNHY 500
            AESWE+VE  VW  LN Y
Sbjct: 902  AESWEQVEQTVWSLLNTY 919

>sp|P09979|KHYB_STRHY HYGROMYCIN-B KINASE (HYGROMYCIN B PHOSPHOTRANSFERASE) (APH(7''))
           gi|66885|pir||WGSMHH hygromycin B phosphotransferase (EC
           2.7.1.-) - Streptomyces hygroscopicus
           gi|581682|emb|CAA27276.1| pot. hyg protein [Streptomyces
           hygroscopicus] gi|1437494|emb|CAA67693.1| hygromycin B
           phosphotransferase [synthetic construct]
           gi|2739064|gb|AAC31623.1| hygromycin B
           phosphotransferase [unidentified cloning vector]
           gi|2739068|gb|AAC31626.1| hygromycin B
           phosphotransferase [unidentified cloning vector]
           gi|19572274|emb|CAD27420.1| hygromycin
           phosphotransferase [Cloning vector pHK9-hygro]
           gi|19572285|emb|CAD27712.1| hygromycin
           phosphotransferase [Cloning vector pHK10-hygro]
           gi|19572287|emb|CAD27713.1| hygromycin
           phosphotransferase [Cloning vector pHK11-hygro]
          Length = 332

 Score = 33.1 bits (74), Expect = 5.1
 Identities = 15/39 (38%), Positives = 23/39 (58%)
 Frame = -1

Query: 820 ILDFSDLSIGDPILDLIPPYLDVFRGDSNLLKRFLESYQ 704
           I+DF+D+  GD    L+  +L+ FRGD  +L   L+  Q
Sbjct: 241 IVDFTDVYAGDSRYSLVQLHLNAFRGDREILAALLDGAQ 279

>ref|XP_222567.1| similar to heat shock protein 86 [Rattus norvegicus]
          Length = 263

 Score = 32.7 bits (73), Expect = 6.6
 Identities = 15/36 (41%), Positives = 21/36 (57%), Gaps = 1/36 (2%)
 Frame = +1

Query: 664 LWTRNSLCY*EKEVGNFLKIASGDW-NHL*THLNKV 768
           +WTR+      K+ G F K  + DW  HL TH+NK+
Sbjct: 184 IWTRSPNDIMNKDYGEFYKSLTNDWEEHLVTHVNKI 219

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 722,587,059
Number of Sequences: 1393205
Number of extensions: 16225908
Number of successful extensions: 36080
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 33965
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36063
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 42296827742
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPDL021a06_f AV777536 1 374 2 GNLf019g12 BP075907 72 532 3 GENLf012f11 BP062988 87 649 4 GNLf019a04 BP075865 108 586 5 MPDL076d04_f AV780406 111 652 6 GENLf016f01 BP063218 133 634 7 GENLf032f05 BP064034 320 829

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