>KMC000309A_C01 KMC000309A_c01
CCGGATAAACACGAAATTTATTAATTAGCATCATCCAAAATACAGGGAAGGAACAAAAAT
CAAGCTTAAAAAAGGGCCCATAACTTTTCCACCCCGTGTACACTTCAAATTTAAGCACTA
AGCATGTTCATTGCACTCATCTAACATATCATTTATTGCAAAAACACATCACACTATGAT
CCCCAAACAACCAAAGAAAATGATAATATAAAATAAACACGCACACATAGATAACATGAA
ATCCCAATGCTCAAACATAGTGGTCCTCATATCATAATATCATAAGCACAATGCTTTCTC
ATATGTGTCGAACTGTAATGGGACTCCTAACTATGTGTCTTCCATTTGTCCATTGCAATT
TCCCAAATAGGTATTTTCTCCTTTGAGTCACACTCGTTGCCTGCACAATAACTTGGAATG
TCTTCTTTTCACCAATTTTCTTGAAACTCAAGGAATTTGGAACAACAACAATTTTATGTC
CTGGCAATTGTGCATTTGCAAAATACTTGCTTGGTGGCCCAACATTGGTGACTGTGCGAG
TAACATTTACCACATTTGACCTGATGTTTGGTAATGTGATTGAAGGGTAGTTCAAGTCTG
TTATGCTGTGAGATCCTGAACAAGTAAATGTCTTGTTGAAGTTTAGTGCTGAAATGAGTT
GTTGGTCATATCCAGAAGCACATAAGAAGTTCAAGTAATCAACAATGCTTAGATCATAAA
CAAGCCCAGGATCTATTGCACTGTTAGGTTGAACATGCCCTGAACCATAAGCAAATGGGG
TT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000309A_C01 KMC000309A_c01
         (782 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAM15440.1| subtilisin-like serine protease AIR3 [Arabidopsis...   164  2e-39
gb|AAK74005.1| At2g04160/T16B23.1 [Arabidopsis thaliana] gi|1632...   164  2e-39
ref|NP_565309.2| subtilisin-like serine protease AIR3; protein i...   164  2e-39
pir||T51335 subtilisin-like proteinase AIR3, auxin-induced [impo...   160  1e-38
gb|AAO61749.1|AF366558_1 subtilisin-like seed-specific protein [...   154  1e-36

>gb|AAM15440.1| subtilisin-like serine protease AIR3 [Arabidopsis thaliana]
          Length = 578

 Score =  164 bits (414), Expect = 2e-39
 Identities = 84/160 (52%), Positives = 112/160 (69%), Gaps = 2/160 (1%)
 Frame = -2

Query: 781 TPFAYGSGHVQPNSAIDPGLVYDLSIVDYLNFLCASGYDQQLISALNFNKTFTCSGSH-S 605
           TPF++G+GHVQPN A++PGLVYDL I DYLNFLC+ GY+   IS  + N  FTCS    S
Sbjct: 419 TPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGN-NFTCSSPKIS 477

Query: 604 ITDLNYPSITLPNIRSNVVNVTRTVTNVGPPSKYFANAQLP-GHKIVVVPNSLSFKKIGE 428
           + +LNYPSIT+PN+ S+ V V+RTV NVG PS Y      P G  + V P SL+F K+GE
Sbjct: 478 LVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGE 537

Query: 427 KKTFQVIVQATSVTQRRKYLFGKLQWTNGRHIVRSPITVR 308
           +KTF+VI+  +     + Y+FG+L W++ +H VRSPI V+
Sbjct: 538 QKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVK 577

>gb|AAK74005.1| At2g04160/T16B23.1 [Arabidopsis thaliana]
           gi|16323308|gb|AAL15409.1| At2g04160/T16B23.1
           [Arabidopsis thaliana]
          Length = 421

 Score =  164 bits (414), Expect = 2e-39
 Identities = 84/160 (52%), Positives = 112/160 (69%), Gaps = 2/160 (1%)
 Frame = -2

Query: 781 TPFAYGSGHVQPNSAIDPGLVYDLSIVDYLNFLCASGYDQQLISALNFNKTFTCSGSH-S 605
           TPF++G+GHVQPN A++PGLVYDL I DYLNFLC+ GY+   IS  + N  FTCS    S
Sbjct: 262 TPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGN-NFTCSSPKIS 320

Query: 604 ITDLNYPSITLPNIRSNVVNVTRTVTNVGPPSKYFANAQLP-GHKIVVVPNSLSFKKIGE 428
           + +LNYPSIT+PN+ S+ V V+RTV NVG PS Y      P G  + V P SL+F K+GE
Sbjct: 321 LVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGE 380

Query: 427 KKTFQVIVQATSVTQRRKYLFGKLQWTNGRHIVRSPITVR 308
           +KTF+VI+  +     + Y+FG+L W++ +H VRSPI V+
Sbjct: 381 QKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVK 420

>ref|NP_565309.2| subtilisin-like serine protease AIR3; protein id: At2g04160.1
            [Arabidopsis thaliana] gi|4218991|gb|AAD12260.1|
            subtilisin-like protease [Arabidopsis thaliana]
          Length = 772

 Score =  164 bits (414), Expect = 2e-39
 Identities = 84/160 (52%), Positives = 112/160 (69%), Gaps = 2/160 (1%)
 Frame = -2

Query: 781  TPFAYGSGHVQPNSAIDPGLVYDLSIVDYLNFLCASGYDQQLISALNFNKTFTCSGSH-S 605
            TPF++G+GHVQPN A++PGLVYDL I DYLNFLC+ GY+   IS  + N  FTCS    S
Sbjct: 613  TPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGN-NFTCSSPKIS 671

Query: 604  ITDLNYPSITLPNIRSNVVNVTRTVTNVGPPSKYFANAQLP-GHKIVVVPNSLSFKKIGE 428
            + +LNYPSIT+PN+ S+ V V+RTV NVG PS Y      P G  + V P SL+F K+GE
Sbjct: 672  LVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGE 731

Query: 427  KKTFQVIVQATSVTQRRKYLFGKLQWTNGRHIVRSPITVR 308
            +KTF+VI+  +     + Y+FG+L W++ +H VRSPI V+
Sbjct: 732  QKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVK 771

>pir||T51335 subtilisin-like proteinase AIR3, auxin-induced [imported] -
            Arabidopsis thaliana (fragment) gi|3695019|gb|AAC62611.1|
            subtilisin-like protease [Arabidopsis thaliana]
          Length = 758

 Score =  160 bits (406), Expect = 1e-38
 Identities = 83/160 (51%), Positives = 110/160 (67%), Gaps = 2/160 (1%)
 Frame = -2

Query: 781  TPFAYGSGHVQPNSAIDPGLVYDLSIVDYLNFLCASGYDQQLISALNFNKTFTCSGSH-S 605
            TPF++G+GHVQPN A++PGLVYDL I DYLNFLC+ GY+   IS  + N  FTCS    S
Sbjct: 599  TPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGN-NFTCSSPKIS 657

Query: 604  ITDLNYPSITLPNIRSNVVNVTRTVTNVGPPSKYFANAQLP-GHKIVVVPNSLSFKKIGE 428
            + +LNYPSIT+PN+ S+ V V+RTV NVG PS Y      P G  + + P SL+F K+GE
Sbjct: 658  LVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPHGVYVALKPTSLNFTKVGE 717

Query: 427  KKTFQVIVQATSVTQRRKYLFGKLQWTNGRHIVRSPITVR 308
             KTF+VI+  +     + Y+FG+L W+  +H VRSPI V+
Sbjct: 718  LKTFKVILVKSKGNVAKGYMFGELVWSAKKHRVRSPIVVK 757

>gb|AAO61749.1|AF366558_1 subtilisin-like seed-specific protein [Arachis hypogaea]
          Length = 244

 Score =  154 bits (390), Expect = 1e-36
 Identities = 77/160 (48%), Positives = 107/160 (66%), Gaps = 2/160 (1%)
 Frame = -2

Query: 781 TPFAYGSGHVQPNSAIDPGLVYDLSIVDYLNFLCASGYDQQLISALNFNKTFTCSGSHSI 602
           TPF+YG+GHV PNSA+DPGLVYD ++ DYLNFLCA GY +  +S       + C+ + SI
Sbjct: 84  TPFSYGAGHVDPNSAMDPGLVYDTTMDDYLNFLCALGYHETQLSMFTEGHHYKCATNFSI 143

Query: 601 TDLNYPSITLPNIRSNVVNVTRTVTNVGPPSKYFANAQLP-GHKIVVVPNSLSFKKIGEK 425
            +LNYPSIT+P    + + +TRT+ NVG P+ Y A  Q P G  + V PN L FK++GE+
Sbjct: 144 LNLNYPSITVPEFPGS-LTLTRTLKNVGAPATYIAQVQNPRGISVFVKPNILEFKEVGEE 202

Query: 424 KTFQVIVQATSVTQRRKYL-FGKLQWTNGRHIVRSPITVR 308
           K+F+V ++      R  +  FGKL W++G H V+SPI V+
Sbjct: 203 KSFEVTLKGRQGEARNNHFKFGKLIWSDGNHYVKSPIVVK 242

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 677,327,685
Number of Sequences: 1393205
Number of extensions: 15236831
Number of successful extensions: 37971
Number of sequences better than 10.0: 165
Number of HSP's better than 10.0 without gapping: 36203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37661
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 38935490438
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPDL067d08_f AV779917 1 506 2 MPDL017c09_f AV777345 1 487 3 MFBL022b06_f BP042337 1 482 4 MFBL034b01_f BP042950 1 490 5 MF006a07_f BP028524 1 407 6 MRL012d09_f BP084314 1 358 7 GENLf074g05 BP066371 1 555 8 MFBL052b08_f BP043917 1 506 9 GENLf011d10 BP062929 2 490 10 GENLf013c06 BP063017 6 488 11 SPDL091f12_f BP057728 12 547 12 SPDL077c12_f BP056772 123 566 13 GENLf028c11 BP063827 134 607 14 GNf018f03 BP068687 136 477 15 GENLf045g01 BP064737 262 790

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