>KMC000217A_C01 KMC000217A_c01
cggtaacaAATGCGGATTAACTATAAAAAAAAACTTTCTTATAACTTTTTTTTTTGTCAG
GAACTCTTGTAAAATTTGTACTAATACAACACATAGATATAAACGAGGCTAGGCATACCC
ACGAGGGGTAACTCAGATAGCAACAGACACAGGATGTGCGGGAAAAGCTCTTGGTTTACA
TTCCCACACAATACACCTCGGGAGAGGGATAAAGAGCCTTGACTAGGAATAAATCTGGAA
TCTGGAATAGTCAGTATCCAAAGGATGACTGGACACCGGGTGTTTATAAAAAAAACGAGG
CTAGGCATAAAAACCTAGAAACGGATAGGGCAAAACAAAAAATGCCATTCTCTATGTTAA
CATAAATTAGCAGGGCAAATCCCCACAGCATACCTAAAACTGCTGCCCCAGAGATAATAC
AGGAGCCGAGCATAAAGATCAAAACCTTATGCCGACTCATGAAATGGCCTATTCTCCAGA
TGCTGCAAGGATGGGAATATTTGTTCATCTTGAAGTAACTTCATTCCTTCAAGCTTTTGC
TAGAAACAGACTGCTG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000217A_C01 KMC000217A_c01
         (556 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_717098.1| glutathione-regulated potassium-efflux system p...    32  5.2
ref|NP_078932.1| hypothetical protein FLJ22329 [Homo sapiens] gi...    31  8.9
dbj|BAC11307.1| unnamed protein product [Homo sapiens]                 31  8.9
dbj|BAC11684.1| unnamed protein product [Homo sapiens]                 31  8.9
gb|AAH20492.1|AAH20492 Similar to hypothetical protein FLJ22329 ...    31  8.9

>ref|NP_717098.1| glutathione-regulated potassium-efflux system protein [Shewanella
           oneidensis MR-1] gi|24347231|gb|AAN54542.1|AE015593_1
           glutathione-regulated potassium-efflux system protein
           [Shewanella oneidensis MR-1]
          Length = 589

 Score = 32.0 bits (71), Expect = 5.2
 Identities = 34/106 (32%), Positives = 48/106 (45%), Gaps = 9/106 (8%)
 Frame = -1

Query: 493 HPCSIWRIGHFMSRHKVLIFMLGS--CIISGAAVLGMLWGFALLIYVNIENGIFCFALSV 320
           +P  +W +       +  IF LGS   ++S AA+ G+ WGF L I   +  G    +LS 
Sbjct: 76  NPSKLWEL-------RSAIFGLGSGQLLLSWAAIGGLAWGFGLSIEAALVVGA-ALSLSS 127

Query: 319 SRF---LCLASFFL*TPGVQSSFGY*LFQ----IPDLFLVKALYPS 203
           + F   L      L TP  + +FG  L Q    IP L L   L P+
Sbjct: 128 TAFAVQLMSEHRLLTTPLGRDAFGVLLMQDLAVIPMLLLTAYLAPN 173

>ref|NP_078932.1| hypothetical protein FLJ22329 [Homo sapiens]
           gi|10438672|dbj|BAB15308.1| unnamed protein product
           [Homo sapiens]
          Length = 243

 Score = 31.2 bits (69), Expect = 8.9
 Identities = 16/37 (43%), Positives = 23/37 (61%)
 Frame = +3

Query: 162 EKLLVYIPTQYTSGEG*RALTRNKSGIWNSQYPKDDW 272
           E LL+Y PT YT  +G  + T   S +WN+++ K DW
Sbjct: 173 EPLLIY-PTHYTGDDGYVSDTET-SVVWNNEHVKTDW 207

>dbj|BAC11307.1| unnamed protein product [Homo sapiens]
          Length = 622

 Score = 31.2 bits (69), Expect = 8.9
 Identities = 16/37 (43%), Positives = 23/37 (61%)
 Frame = +3

Query: 162 EKLLVYIPTQYTSGEG*RALTRNKSGIWNSQYPKDDW 272
           E LL+Y PT YT  +G  + T   S +WN+++ K DW
Sbjct: 552 EPLLIY-PTHYTGDDGYVSDTET-SVVWNNEHVKTDW 586

>dbj|BAC11684.1| unnamed protein product [Homo sapiens]
          Length = 622

 Score = 31.2 bits (69), Expect = 8.9
 Identities = 16/37 (43%), Positives = 23/37 (61%)
 Frame = +3

Query: 162 EKLLVYIPTQYTSGEG*RALTRNKSGIWNSQYPKDDW 272
           E LL+Y PT YT  +G  + T   S +WN+++ K DW
Sbjct: 552 EPLLIY-PTHYTGDDGYVSDTET-SVVWNNEHVKTDW 586

>gb|AAH20492.1|AAH20492 Similar to hypothetical protein FLJ22329 [Homo sapiens]
          Length = 231

 Score = 31.2 bits (69), Expect = 8.9
 Identities = 16/37 (43%), Positives = 23/37 (61%)
 Frame = +3

Query: 162 EKLLVYIPTQYTSGEG*RALTRNKSGIWNSQYPKDDW 272
           E LL+Y PT YT  +G  + T   S +WN+++ K DW
Sbjct: 161 EPLLIY-PTHYTGDDGYVSDTET-SVVWNNEHVKTDW 195

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 478,310,304
Number of Sequences: 1393205
Number of extensions: 10492452
Number of successful extensions: 26204
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 25587
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26199
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19521267756
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf040e03 BP070315 1 319 2 GENLf060h06 BP065582 7 555 3 GNLf001c11 BP074842 19 329 4 GENLf092f06 BP067391 19 501 5 GENLf047e09 BP064844 19 570 6 MPDL057c11_f AV779387 19 572 7 GNLf007f08 BP075224 20 444 8 GNLf008c01 BP075265 23 546 9 GENLf092c05 BP067366 23 464 10 MPDL061e08_f AV779608 57 544 11 MPDL089c12_f AV781142 57 549 12 MRL047b07_f BP085965 57 480 13 MRL039f08_f BP085645 58 391 14 MWM168c11_f AV767329 58 472 15 MRL009e03_f BP084161 58 504 16 GENLf008d01 BP062759 180 545

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