Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000178A_C03 KMC000178A_c03
(587 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_201416.1| glycosyl hydrolase family 38 (alpha-mannosidase... 162 3e-39
ref|NP_189306.1| glycosyl hydrolase family 38 (alpha-mannosidase... 143 2e-33
gb|AAL73069.1|AC090873_15 Putative alpha-mannosidase [Oryza sati... 136 2e-31
dbj|BAB11126.1| alpha-mannosidase [Arabidopsis thaliana] 118 5e-26
ref|NP_196902.2| glycosyl hydrolase family 38 (alpha-mannosidase... 118 5e-26
>ref|NP_201416.1| glycosyl hydrolase family 38 (alpha-mannosidase); protein id:
At5g66150.1 [Arabidopsis thaliana]
gi|10177130|dbj|BAB10420.1| alpha-mannosidase
[Arabidopsis thaliana]
Length = 1047
Score = 162 bits (410), Expect = 3e-39
Identities = 86/143 (60%), Positives = 104/143 (72%), Gaps = 1/143 (0%)
Frame = -1
Query: 587 SPLLLAFTHENLENWKSSHLTEGTVMDPNYTLPPNVALITLEVLDGGVVLLRLAHLYETN 408
SPLL+AF HEN E WK+S+ +G MD YTLP N+ALITLE LD G VLLRLAHLYE
Sbjct: 892 SPLLMAFAHENKEKWKASNTVKGYAMDHLYTLPQNIALITLEELDLGNVLLRLAHLYEAG 951
Query: 407 EDAEYSTLAQVELKKLFASKTIKELKEVSLSINPEKSEMK-KMTWKVEGDIGQEPQAVRG 231
ED++YS +A+VELKKLF+ K IKE+ E+SLS N EK +MK KM WKVEG+ Q +RG
Sbjct: 952 EDSDYSKIAKVELKKLFSGKMIKEVTEMSLSANQEKVKMKEKMKWKVEGEAEQPSSPLRG 1011
Query: 230 GAVRTTDFVVELGPMAHSYFLVE 162
G V + VVELGPM F+V+
Sbjct: 1012 GPVDKSTLVVELGPMEIRTFVVQ 1034
>ref|NP_189306.1| glycosyl hydrolase family 38 (alpha-mannosidase); protein id:
At3g26720.1, supported by cDNA: gi_14517402 [Arabidopsis
thaliana] gi|1888357|emb|CAA66821.1| alpha-mannosidase
[Arabidopsis thaliana] gi|1890154|emb|CAA72432.1|
alpha-mannosidase precursor [Arabidopsis thaliana]
gi|11994305|dbj|BAB01735.1| alpha-mannosidase
[Arabidopsis thaliana] gi|14517403|gb|AAK62592.1|
AT3g26720/MLJ15_12 [Arabidopsis thaliana]
gi|21360397|gb|AAM47314.1| AT3g26720/MLJ15_12
[Arabidopsis thaliana]
Length = 1019
Score = 143 bits (360), Expect = 2e-33
Identities = 77/144 (53%), Positives = 103/144 (71%), Gaps = 2/144 (1%)
Frame = -1
Query: 587 SPLLLAFTHENLENWKSSHLTEGTVMDPNYTLPPNVALITLEVLDGGVVLLRLAHLYETN 408
SPLL+AFT + ++W +SH T + +P+Y+LP NVAL+TL+ L+ G VLLRLAHL+E
Sbjct: 861 SPLLIAFTEQEGDSWINSHKTTFSAFEPSYSLPKNVALLTLQELENGEVLLRLAHLFEVG 920
Query: 407 EDAEYSTLAQVELKKLFASKTIKELKEVSLSINPEKSEMKK--MTWKVEGDIGQEPQAVR 234
ED+EYS +A+VELKKLF +K I+E+KE SLS N EK+EM+K + WKVEG G+E + R
Sbjct: 921 EDSEYSVMAKVELKKLFHNKKIREVKETSLSGNQEKAEMEKRRLIWKVEGSAGEEVK--R 978
Query: 233 GGAVRTTDFVVELGPMAHSYFLVE 162
G AV VVEL PM L++
Sbjct: 979 GEAVDAEKLVVELVPMEIRTLLIK 1002
>gb|AAL73069.1|AC090873_15 Putative alpha-mannosidase [Oryza sativa]
gi|19919971|gb|AAM08419.1|AC112513_5 Putative
alpha-mannosidase [Oryza sativa]
Length = 438
Score = 136 bits (342), Expect = 2e-31
Identities = 64/129 (49%), Positives = 93/129 (71%)
Frame = -1
Query: 548 NWKSSHLTEGTVMDPNYTLPPNVALITLEVLDGGVVLLRLAHLYETNEDAEYSTLAQVEL 369
+WKS+++ + + ++ NY+LP NVA+ITL+ LD G LLRLAHL++ ED +YS +A+VEL
Sbjct: 309 SWKSNNIAKASTVEGNYSLPDNVAIITLQSLDDGTTLLRLAHLFQAQEDTQYSVMAKVEL 368
Query: 368 KKLFASKTIKELKEVSLSINPEKSEMKKMTWKVEGDIGQEPQAVRGGAVRTTDFVVELGP 189
+KLF + IK+L E SLS N +KSEMKK+ W+V G+ +P ++GG V + VVELGP
Sbjct: 369 RKLFGKRIIKDLTETSLSANQKKSEMKKLNWRVTGESKTDPAPLKGGPVDSHALVVELGP 428
Query: 188 MAHSYFLVE 162
M FL++
Sbjct: 429 MEIRTFLLK 437
>dbj|BAB11126.1| alpha-mannosidase [Arabidopsis thaliana]
Length = 1030
Score = 118 bits (296), Expect = 5e-26
Identities = 71/147 (48%), Positives = 92/147 (62%), Gaps = 4/147 (2%)
Frame = -1
Query: 587 SPLLLAFTHENLENWKSSHLTEGTVMDPNYTLPPNVALITLEVLDGGVVLLRLAHLYETN 408
SPLLLAF ++ S + +DP+Y+LP NVAL+TL+ LD G VLLRLAHLYE
Sbjct: 870 SPLLLAFAQQDDGKPMSFGAASFSGIDPSYSLPDNVALLTLQELDDGNVLLRLAHLYEVE 929
Query: 407 EDAEYSTLAQVELKKLFASKTIKELKEVSLSINPEKS--EMKKMTWKVEGD--IGQEPQA 240
ED E S +A VELKKLF K I +L E+SLS N E+S E K++ WKVEG+ G+E +A
Sbjct: 930 EDKELSGVASVELKKLFPGKKIGKLTEMSLSANQERSTMEKKRLVWKVEGEGSYGEEKKA 989
Query: 239 VRGGAVRTTDFVVELGPMAHSYFLVEI 159
RG + +EL PM L+ +
Sbjct: 990 KRGREIDPRKLEMELYPMEIRTVLIHL 1016
>ref|NP_196902.2| glycosyl hydrolase family 38 (alpha-mannosidase); protein id:
At5g13980.1, supported by cDNA: gi_20466475 [Arabidopsis
thaliana] gi|20466476|gb|AAM20555.1| alpha-mannosidase
[Arabidopsis thaliana] gi|23198186|gb|AAN15620.1|
alpha-mannosidase [Arabidopsis thaliana]
Length = 1024
Score = 118 bits (296), Expect = 5e-26
Identities = 71/147 (48%), Positives = 92/147 (62%), Gaps = 4/147 (2%)
Frame = -1
Query: 587 SPLLLAFTHENLENWKSSHLTEGTVMDPNYTLPPNVALITLEVLDGGVVLLRLAHLYETN 408
SPLLLAF ++ S + +DP+Y+LP NVAL+TL+ LD G VLLRLAHLYE
Sbjct: 864 SPLLLAFAQQDDGKPMSFGAASFSGIDPSYSLPDNVALLTLQELDDGNVLLRLAHLYEVE 923
Query: 407 EDAEYSTLAQVELKKLFASKTIKELKEVSLSINPEKS--EMKKMTWKVEGD--IGQEPQA 240
ED E S +A VELKKLF K I +L E+SLS N E+S E K++ WKVEG+ G+E +A
Sbjct: 924 EDKELSGVASVELKKLFPGKKIGKLTEMSLSANQERSTMEKKRLVWKVEGEGSYGEEKKA 983
Query: 239 VRGGAVRTTDFVVELGPMAHSYFLVEI 159
RG + +EL PM L+ +
Sbjct: 984 KRGREIDPRKLEMELYPMEIRTVLIHL 1010
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 485,705,167
Number of Sequences: 1393205
Number of extensions: 9841380
Number of successful extensions: 26001
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 25145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25990
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 22283372436
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
