>KMC000174A_C01 KMC000174A_c01
atcagaatcaacttgtataggcatgaagatcatacttgatgAAATATATAAGATAACACC
TATAAAATTAAGCAAATCAATAAGCACCATGCACTTGGTTGCAATATTCTAAAAAAGACC
AACAAAAGACTAATCAGCTCAAGTACACCAATTCTAATGTGGCACACTGCCACTACAATG
CTAACAACAGAATTGATGGTTCCATTTAGAGAAGCCACACGACCCACACGTTCAGAAAAT
TCGAGCAACAACACCAAACAATCAGGCAGGGAATAATACTTGACCGGACATGGCAGATAA
TGCATCTTAACCCACTTTTTATAGCAACCATAATCAATAACTTCAGTTATTCAAGCTCAG
GGTTAGGGTTAATTTCGGTAGAGATCCTAAGGGCATATCAGCGGGTTGTCCTTCGAAATC
TCCATGAAAGATTTTGCGGCGGCGGTAGATTCGTATGACAGAGAAGATCAGTAGGAGAGA
GTCGTACTTGTTCTACCTGGTAGCTGTATCAGATCATTAAAAAGTAACACATAGAAGACA
ACCAAAGTA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000174A_C01 KMC000174A_c01
         (549 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_501436.1| Putative plasma membrane membrane protein, with...    34  1.3
ref|NP_681197.1| ORF_ID:tlr0407~unknown protein [Thermosynechoco...    32  3.9
ref|NP_704685.1| hypothetical protein [Plasmodium falciparum 3D7...    32  3.9
gb|EAA19568.1| hypothetical protein [Plasmodium yoelii yoelii]         32  5.0
dbj|BAB09379.1| non-LTR retroelement reverse transcriptase-like ...    32  5.0

>ref|NP_501436.1| Putative plasma membrane membrane protein, with at least 10
           transmembrane domains, a coiled coil domain, nematode
           specific (77.4 kD) [Caenorhabditis elegans]
           gi|7508481|pir||T29669 hypothetical protein T26A8.1 -
           Caenorhabditis elegans gi|1166612|gb|AAA85757.1|
           Hypothetical protein T26A8.1 [Caenorhabditis elegans]
          Length = 708

 Score = 33.9 bits (76), Expect = 1.3
 Identities = 32/104 (30%), Positives = 45/104 (42%), Gaps = 2/104 (1%)
 Frame = -3

Query: 346 LKLLIMVA--IKSGLRCIICHVRSSIIPCLIVWCCCSNFLNVWVVWLL*MEPSILLLAL* 173
           L LL ++A  + S     +C VR  +I CL  W C   F   W   L     +++L  L 
Sbjct: 89  LLLLFLIAGLVSSSTTLSVCSVRHEVIACL--WTCIGMFCGAWSTKLTSARSNLMLFLL- 145

Query: 172 WQCATLELVYLS*LVFCWSFLEYCNQVHGAY*FA*FYRCYLIYF 41
              A L L+    L F ++FL     V G +  A  Y   LI+F
Sbjct: 146 --SALLTLLVNLFLAFNFAFL---FAVRGFFVGATLYGGALIWF 184

>ref|NP_681197.1| ORF_ID:tlr0407~unknown protein [Thermosynechococcus elongatus BP-1]
           gi|22294128|dbj|BAC07959.1| ORF_ID:tlr0407~unknown
           protein [Thermosynechococcus elongatus BP-1]
          Length = 277

 Score = 32.3 bits (72), Expect = 3.9
 Identities = 20/57 (35%), Positives = 25/57 (43%), Gaps = 6/57 (10%)
 Frame = +3

Query: 225 HTFRKFEQQHQTIRQGIILDRTWQIMHLNP------LFIATIINNFSYSSSGLGLIS 377
           HT +    +H  +R G  L   WQI    P      L +ATI N    S  GLG I+
Sbjct: 7   HTPQAIANRHYQVRSGQYLKEGWQIFSTQPAAYIGFLVLATITNTLLRSIPGLGPIA 63

>ref|NP_704685.1| hypothetical protein [Plasmodium falciparum 3D7]
            gi|23505045|emb|CAD51828.1| hypothetical protein
            [Plasmodium falciparum 3D7]
          Length = 1728

 Score = 32.3 bits (72), Expect = 3.9
 Identities = 15/46 (32%), Positives = 22/46 (47%)
 Frame = +3

Query: 117  DQQKTNQLKYTNSNVAHCHYNANNRIDGSI*RSHTTHTFRKFEQQH 254
            D QK NQ+K  N N+ +  YN N+ +D       T   +  FE  +
Sbjct: 1267 DVQKNNQIKENNDNIINVPYNNNSNVDEGYNEDITEKKYITFENHN 1312

>gb|EAA19568.1| hypothetical protein [Plasmodium yoelii yoelii]
          Length = 5074

 Score = 32.0 bits (71), Expect = 5.0
 Identities = 25/91 (27%), Positives = 45/91 (48%), Gaps = 5/91 (5%)
 Frame = +3

Query: 108 SKKDQQKTNQLKYTN-SNVAHCHYNANNRIDGSI*RSHTTHTF----RKFEQQHQTIRQG 272
           ++K  ++TN    +N +N  +C+YN  N     + R    H F     +FE  H  I Q 
Sbjct: 237 TEKCNEETNFDPNSNVNNSENCNYNNTN-----MNRQKNDHNFLSHQNQFEYNHNIIDQS 291

Query: 273 IILDRTWQIMHLNPLFIATIINNFSYSSSGL 365
           I  +  +++   N + I +I ++ SY+SS +
Sbjct: 292 IKTNSNYKLSDYNKIHICSITSD-SYNSSSI 321

>dbj|BAB09379.1| non-LTR retroelement reverse transcriptase-like protein
           [Arabidopsis thaliana]
          Length = 1223

 Score = 32.0 bits (71), Expect = 5.0
 Identities = 18/58 (31%), Positives = 29/58 (49%), Gaps = 4/58 (6%)
 Frame = -2

Query: 296 LPCPVKYYSLPDCLVLLLEFSERVG----RVASLNGTINSVVSIVVAVCHIRIGVLEL 135
           LP   K  S  DCL LL +  +R+G    R  S  G +N + S++ ++C+  +    L
Sbjct: 774 LPLITKRLSTTDCLPLLEQVRKRIGSWTSRFLSYAGRLNLISSVLWSICNFWLAAFRL 831

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 446,844,439
Number of Sequences: 1393205
Number of extensions: 9227652
Number of successful extensions: 22375
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 21688
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22363
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 18947112822
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENLf029c01 BP063865 1 524 2 MFBL012b03_f BP041853 42 501 3 GENLf006g10 BP062680 50 549 4 GENLf078c09 BP066589 62 549 5 SPDL003g12_f BP052199 71 546

---