>KMC000147A_C02 KMC000147A_c02
AGTAAACAATCAATACTAGATCATCACGAGGTCATCTTACAGAACTTACAAACCAGTCAT
TTTAACACAGGGAAGTTAAAAGGGCTTCCATGGTTTACTCACGACAATCATGAATACAAG
GCATATATCCAAAAGAATCAAGCAGAATAAAACAATCAAATTTTACAGTACGGCGCCTAA
AGCAGGGCCTAGATCCTCTAAGCAGGGCCTAGATCATCTTCTATATACACGGGACTCGGC
ACACCTCCTGGCATTCAAACAAATTCAAAACAAAATAATAATGAATAGAAGAAACCAACT
GGTACGCTATAAAGGCTATGCACACCAACAATGAAAAAGGAAGCAAACATGTAAATTCTG
CATCTGTCCCATTATTTGACTTGAATGTCTCCCTGGGCCTCCGGGGATAGGTTCTGGGAG
GATGGAGATTTCCCAGTGATAGCTTTCTTGGCTTTGGCTAGTGACTGTTTCACCTTTGAT
ATAATGTTGTTGGATGGCTTTGGAGCTGGCTGGGCTGGAGTTTCTTTGGGAAGGTCTTGG
GATGTTTTGGTTACATCACTTTCCTTGGTGTCTTGAACCTCAGTTTTTACAGGCTCCTCA
ACTTGTTCTTTCTCTGTATTATCCACTCCAGTTTCAATAGATTCCTCATCTTTAAACTTn
GATGCCAATGTTTCTCTAACAGGTTCACTTATGGGAGGGGAGATTTCATCCACCTTTGTG
TGAGCATTCTCTTCTTTCT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000147A_C02 KMC000147A_c02
         (739 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_187241.1| unknown protein; protein id: At3g05900.1 [Arabi...    64  2e-09
gb|EAA21432.1| hypothetical protein [Plasmodium yoelii yoelii]         44  0.002
ref|NP_279749.1| Vng0754c [Halobacterium sp. NRC-1] gi|25409633|...    43  0.005
emb|CAC35772.3| dhn1 [Populus euramericana]                            41  0.015
ref|NP_609020.2| CG9506-PA [Drosophila melanogaster] gi|22945762...    41  0.020

>ref|NP_187241.1| unknown protein; protein id: At3g05900.1 [Arabidopsis thaliana]
           gi|6671967|gb|AAF23226.1|AC013454_13 unknown protein
           [Arabidopsis thaliana]
           gi|6714403|gb|AAF26092.1|AC012393_18 unknown protein
           [Arabidopsis thaliana]
          Length = 660

 Score = 64.3 bits (155), Expect = 2e-09
 Identities = 49/116 (42%), Positives = 65/116 (55%), Gaps = 5/116 (4%)
 Frame = -3

Query: 731 ENAHTKVDEISPPISEPVRETLASKFKDEESIET-GVDNTEKEQVEEPVKTEVQDT---K 564
           E+ + K D  +P       ETL  K  D ES+E     N ++E + E V   V+     K
Sbjct: 541 EDENIKKDTDTPVAEGKSEETL--KETDTESVEKEAAANKQEEPITEKVAEVVETAPVAK 598

Query: 563 ESDVTKTSQDLP-KETPAQPAPKPSNNIISKVKQSLAKAKKAITGKSPSSQNLSPE 399
           E D  K   ++  KE PA+   K SN+IISKVKQSL KAKKAI G+SPSS+ ++ E
Sbjct: 599 EIDEAKQQPEVTTKEAPAKQ--KHSNSIISKVKQSLVKAKKAIIGRSPSSKTITTE 652

>gb|EAA21432.1| hypothetical protein [Plasmodium yoelii yoelii]
          Length = 1013

 Score = 44.3 bits (103), Expect = 0.002
 Identities = 30/129 (23%), Positives = 60/129 (46%), Gaps = 10/129 (7%)
 Frame = -3

Query: 737 KEENAHTKVDEISPPISEPVRETLASKFKDE------ESIETGVDNTEKEQVEEPVKTEV 576
           KEE      DE+   I E V+E +  + K+E      E ++  + +  KE+++E +K E+
Sbjct: 507 KEEIKEEIKDEVKEEIKEEVKEEIKEEVKEEIKEEIKEEVKEEIKDEVKEEIKEEIKEEI 566

Query: 575 QDTKESDVTKT-SQDLPKETPAQPAPKPSNNIISKVKQSL---AKAKKAITGKSPSSQNL 408
           QD  + D+ +   +++ +E   +   +    I  +VK+ +    K +    GK      +
Sbjct: 567 QDEGKEDIKEEGKEEIKEEGKEEIKEEVKEEIKDEVKEEIKEEIKEEIKYEGKEEIKDEI 626

Query: 407 SPEAQGDIQ 381
             E + +IQ
Sbjct: 627 KDEGKEEIQ 635

 Score = 35.4 bits (80), Expect = 0.85
 Identities = 18/72 (25%), Positives = 39/72 (54%)
 Frame = -3

Query: 737 KEENAHTKVDEISPPISEPVRETLASKFKDEESIETGVDNTEKEQVEEPVKTEVQDTKES 558
           KEE      DE+   I E ++E +  K++ +E I+  + +  KE++++  K E++D  + 
Sbjct: 591 KEEVKEEIKDEVKEEIKEEIKEEI--KYEGKEEIKDEIKDEGKEEIQDEGKEEIKDEGKE 648

Query: 557 DVTKTSQDLPKE 522
           ++    ++  KE
Sbjct: 649 EIQDEGKEEIKE 660

>ref|NP_279749.1| Vng0754c [Halobacterium sp. NRC-1] gi|25409633|pir||A84233
           hypothetical protein Vng0754c [imported] - Halobacterium
           sp. NRC-1 gi|10580333|gb|AAG19229.1| Vng0754c
           [Halobacterium sp. NRC-1]
          Length = 267

 Score = 42.7 bits (99), Expect = 0.005
 Identities = 25/75 (33%), Positives = 43/75 (57%), Gaps = 3/75 (4%)
 Frame = -3

Query: 734 EENAHTKVDE-ISPPISEPVRETLASKFKD--EESIETGVDNTEKEQVEEPVKTEVQDTK 564
           +E+  + VDE +   + + V ET++   ++  +ES+E  VD T  E VEE VK +V+++ 
Sbjct: 166 KESVESTVDETVGETVEQTVDETVSETVEETVKESVEETVDETVSETVEESVKQQVEESV 225

Query: 563 ESDVTKTSQDLPKET 519
              V +T +   KET
Sbjct: 226 NETVEETVEQTVKET 240

 Score = 37.4 bits (85), Expect = 0.22
 Identities = 24/77 (31%), Positives = 38/77 (49%), Gaps = 2/77 (2%)
 Frame = -3

Query: 731 ENAHTKVDEISPPISEPVRETLASKFKD--EESIETGVDNTEKEQVEEPVKTEVQDTKES 558
           E     VDE    +SE V ET+    ++  +E++   V+ + K+QVEE V   V++T E 
Sbjct: 179 ETVEQTVDET---VSETVEETVKESVEETVDETVSETVEESVKQQVEESVNETVEETVEQ 235

Query: 557 DVTKTSQDLPKETPAQP 507
            V +T  +       QP
Sbjct: 236 TVKETVDERLAAADVQP 252

>emb|CAC35772.3| dhn1 [Populus euramericana]
          Length = 225

 Score = 41.2 bits (95), Expect = 0.015
 Identities = 35/134 (26%), Positives = 61/134 (45%), Gaps = 13/134 (9%)
 Frame = -3

Query: 737 KEENAHTKVDEISPPISEPVRETLASKFKDEESIETGVDNT------EKEQVEEPVKTEV 576
           +EE+   + +E  P + E +  + +S        E G D        EK  ++E +K   
Sbjct: 63  EEEHKKKEEEEKKPTLFEKLHRSGSSSSSSSSDEEEGDDEEKKKKKKEKRSLKEKMKISG 122

Query: 575 QDTKESDVTKTS---QDLPKETPAQPAPKPSNNIISKVKQSLAKAKKAITGKSPSSQNLS 405
           +  +E +   TS   + +  ETP +P  K     + K+K+ L   KKA     P+ +++S
Sbjct: 123 EKGEEKEHEDTSVPVEVVHTETPHEPEDK--KGFLDKIKEKLPGHKKADEVPPPAPEHVS 180

Query: 404 PEA----QGDIQVK 375
           PEA    +GD + K
Sbjct: 181 PEAAVSHEGDAKEK 194

>ref|NP_609020.2| CG9506-PA [Drosophila melanogaster] gi|22945762|gb|AAF52374.2|
           CG9506-PA [Drosophila melanogaster]
          Length = 1196

 Score = 40.8 bits (94), Expect = 0.020
 Identities = 27/114 (23%), Positives = 52/114 (44%), Gaps = 8/114 (7%)
 Frame = -3

Query: 704 ISPPISEPVRETL--------ASKFKDEESIETGVDNTEKEQVEEPVKTEVQDTKESDVT 549
           +  P+ EP   TL          +  +E   +  +DN  K++ +   K E+   ++  V 
Sbjct: 494 LEQPLLEPSTATLEDVSQKSAVERLLEEAIAKLELDNESKKETQVEEKKEMDTAQDPIVQ 553

Query: 548 KTSQDLPKETPAQPAPKPSNNIISKVKQSLAKAKKAITGKSPSSQNLSPEAQGD 387
            T + + ++ P  PAP+PS   +S+V  S +  K++   +S S  +  P+   D
Sbjct: 554 VTPRRIKRQAPPVPAPRPS---LSQVTSSASSCKQSEAEESESGLSTLPKITSD 604

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 658,952,662
Number of Sequences: 1393205
Number of extensions: 15310387
Number of successful extensions: 58839
Number of sequences better than 10.0: 439
Number of HSP's better than 10.0 without gapping: 53196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 58102
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 35188080875
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENLf069b10 BP066060 1 281 2 GENLf033c11 BP064063 39 462 3 MRL029e10_f BP085175 64 478 4 GENLf012e11 BP062980 65 497 5 GENLf014a10 BP063062 65 470 6 MPDL038g12_f AV778450 100 489 7 GENLf059b07 BP065490 111 578 8 GENLf005f10 BP062623 150 675 9 GENLf077f11 BP066549 270 753

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