Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000144A_C01 KMC000144A_c01
(680 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_179660.1| glycosyl hydrolase family 5/cellulase ((1-4)-be... 134 4e-38
ref|NP_194561.1| glycosyl hydrolase family 5/cellulase ((1-4)-be... 128 9e-37
dbj|BAB56016.1| putative (1-4)-beta-mannan endohydrolase [Oryza ... 100 1e-26
ref|NP_201447.1| glycosyl hydrolase family 5/cellulase ((1-4)-be... 89 6e-20
emb|CAC51690.2| endo-beta-1,4-mannanase [Lactuca sativa] 77 4e-17
>ref|NP_179660.1| glycosyl hydrolase family 5/cellulase ((1-4)-beta-mannan
endohydrolase); protein id: At2g20680.1 [Arabidopsis
thaliana] gi|25354515|pir||A84592 (1-4)-beta-mannan
endohydrolase [imported] - Arabidopsis thaliana
gi|4454480|gb|AAD20927.1| (1-4)-beta-mannan
endohydrolase [Arabidopsis thaliana]
Length = 403
Score = 134 bits (338), Expect(2) = 4e-38
Identities = 60/108 (55%), Positives = 85/108 (78%)
Frame = -1
Query: 581 KFVSEWMLSHIENGDKVLNKPVLFSEYGLSQRVHNFSASDREQTHKTILDIIYKSAKRNK 402
KFV +WMLSHIE+GDK L KPVLF+E+GLS ++ S R++ ++TI D+IYKSAKR +
Sbjct: 280 KFVVKWMLSHIEDGDKELKKPVLFTEFGLSNLNKDYDPSQRDRFYRTIFDVIYKSAKRKR 339
Query: 401 SGAGTLIWQFLVDGINEFSDEYGMVPGENSSTYSLLLQQSCRLAKA*G 258
SGAGTL+WQFL++G+ F+D++G+VP E S L+++QSCRL++ G
Sbjct: 340 SGAGTLVWQFLIEGMEGFNDDFGIVPWEQDSIQRLMIEQSCRLSRITG 387
Score = 45.8 bits (107), Expect(2) = 4e-38
Identities = 20/34 (58%), Positives = 24/34 (69%)
Frame = -3
Query: 678 RNSKISNIDFTSVHIYGDQWSYKQEFEDQLKFQI 577
RNS NIDF SVHIY D W + Q FE++LKF +
Sbjct: 250 RNSDSPNIDFASVHIYPDHWFHDQGFEEKLKFVV 283
>ref|NP_194561.1| glycosyl hydrolase family 5/cellulase ((1-4)-beta-mannan
endohydrolase); protein id: At4g28320.1 [Arabidopsis
thaliana] gi|7488130|pir||T09048 probable mannan
endo-1,4-beta-mannosidase (EC 3.2.1.78) - Arabidopsis
thaliana gi|7269686|emb|CAB79634.1| putative
(1-4)-beta-mannan endohydrolase [Arabidopsis thaliana]
Length = 431
Score = 128 bits (322), Expect(2) = 9e-37
Identities = 57/108 (52%), Positives = 81/108 (74%)
Frame = -1
Query: 581 KFVSEWMLSHIENGDKVLNKPVLFSEYGLSQRVHNFSASDREQTHKTILDIIYKSAKRNK 402
KFV +WM SHIE+G K L KPVLF+E+GLS + ++ S R++ ++ I D++YKSAKR K
Sbjct: 309 KFVVKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQRDKFYRIIFDVVYKSAKRKK 368
Query: 401 SGAGTLIWQFLVDGINEFSDEYGMVPGENSSTYSLLLQQSCRLAKA*G 258
SGAGTL+WQ ++G+ F+D++G+VP E S Y L+++QSCRL K G
Sbjct: 369 SGAGTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSCRLGKVTG 416
Score = 47.4 bits (111), Expect(2) = 9e-37
Identities = 20/34 (58%), Positives = 25/34 (72%)
Frame = -3
Query: 678 RNSKISNIDFTSVHIYGDQWSYKQEFEDQLKFQI 577
+NS SNIDF SVHIY D W + Q FE++LKF +
Sbjct: 279 QNSNSSNIDFASVHIYPDHWFHNQTFEEKLKFVV 312
>dbj|BAB56016.1| putative (1-4)-beta-mannan endohydrolase [Oryza sativa (japonica
cultivar-group)] gi|21902051|dbj|BAC05600.1| putative
(1-4)-beta-mannan endohydrolase [Oryza sativa (japonica
cultivar-group)]
Length = 445
Score = 99.8 bits (247), Expect(2) = 1e-26
Identities = 42/104 (40%), Positives = 68/104 (65%)
Frame = -1
Query: 581 KFVSEWMLSHIENGDKVLNKPVLFSEYGLSQRVHNFSASDREQTHKTILDIIYKSAKRNK 402
KF++ W+ +H+E+GD L KPVL +E+GLS +V F + R+ ++ + DI++ SA+R
Sbjct: 324 KFMTRWITAHVEDGDGELEKPVLVTEFGLSHQVEGFEDAHRDVLYRAVYDIVHGSARRGG 383
Query: 401 SGAGTLIWQFLVDGINEFSDEYGMVPGENSSTYSLLLQQSCRLA 270
+ G L+WQ +G+ E+ D + +VP E S L+ +QSCRLA
Sbjct: 384 AAGGALVWQLAAEGMEEYHDGFSIVPSERPSMMRLIKEQSCRLA 427
Score = 42.0 bits (97), Expect(2) = 1e-26
Identities = 17/32 (53%), Positives = 22/32 (68%)
Frame = -3
Query: 678 RNSKISNIDFTSVHIYGDQWSYKQEFEDQLKF 583
RNSKI +IDF SVH+Y D W +++LKF
Sbjct: 294 RNSKIQDIDFASVHVYPDNWLQHASLDEKLKF 325
>ref|NP_201447.1| glycosyl hydrolase family 5/cellulase ((1-4)-beta-mannan
endohydrolase); protein id: At5g66460.1 [Arabidopsis
thaliana] gi|10177527|dbj|BAB10922.1| mannan
endo-1,4-beta-mannosidase [Arabidopsis thaliana]
gi|23306404|gb|AAN17429.1| mannan
endo-1,4-beta-mannosidase [Arabidopsis thaliana]
Length = 431
Score = 88.6 bits (218), Expect(2) = 6e-20
Identities = 39/102 (38%), Positives = 68/102 (66%)
Frame = -1
Query: 578 FVSEWMLSHIENGDKVLNKPVLFSEYGLSQRVHNFSASDREQTHKTILDIIYKSAKRNKS 399
F+++W+ +HI++ VL+KP++ +E+G S + ++ + R+ T+ IY SAKR +
Sbjct: 296 FLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKPGYTPAQRDIVFNTVYSKIYGSAKRGGA 355
Query: 398 GAGTLIWQFLVDGINEFSDEYGMVPGENSSTYSLLLQQSCRL 273
AG L WQ LV+GI+ F D YG++ ++SST +++ QQS +L
Sbjct: 356 AAGGLFWQLLVNGIDNFQDGYGIILSQSSSTVNVISQQSRKL 397
Score = 30.8 bits (68), Expect(2) = 6e-20
Identities = 12/31 (38%), Positives = 18/31 (57%)
Frame = -3
Query: 675 NSKISNIDFTSVHIYGDQWSYKQEFEDQLKF 583
N++I IDF +VH Y D+W + Q+ F
Sbjct: 266 NNRIPGIDFVTVHSYPDEWFPDSSEQSQMDF 296
>emb|CAC51690.2| endo-beta-1,4-mannanase [Lactuca sativa]
Length = 410
Score = 77.0 bits (188), Expect(2) = 4e-17
Identities = 35/104 (33%), Positives = 64/104 (60%)
Frame = -1
Query: 581 KFVSEWMLSHIENGDKVLNKPVLFSEYGLSQRVHNFSASDREQTHKTILDIIYKSAKRNK 402
KFV +W+ +HIE+ D +L KP+L +E+G S ++ R++ I + Y+SA+
Sbjct: 291 KFVEKWINAHIEDCDSILRKPLLIAEFGKSSWSSGYTVEARDEYFGGIFNTAYESARNRG 350
Query: 401 SGAGTLIWQFLVDGINEFSDEYGMVPGENSSTYSLLLQQSCRLA 270
S +GT WQ + +G++ + D Y +V +N ST +++ +QS R++
Sbjct: 351 SCSGTTFWQVMAEGMDNWGDGYQVVLDQNPSTAAIIAKQSQRIS 394
Score = 32.7 bits (73), Expect(2) = 4e-17
Identities = 12/31 (38%), Positives = 20/31 (63%)
Frame = -3
Query: 675 NSKISNIDFTSVHIYGDQWSYKQEFEDQLKF 583
N+ ++N+DF ++H+Y DQW E + KF
Sbjct: 262 NNGVNNVDFATIHMYPDQWVPGASDEARAKF 292
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 562,081,535
Number of Sequences: 1393205
Number of extensions: 11858891
Number of successful extensions: 41376
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 40499
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 41364
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 30270070164
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
