>KMC000105A_C01 KMC000105A_c01
atggctataatgactacagggtcacagagtgtagctcagaggttttaccaggcattcttc
aaggggacaagtcagactctCTCTTGTATGGCTCTGTTGACAATGGGAAAAAAATTTGCT
GGAATGAAGATTTTCATTCTAAGGTATCTGAGGCTGCCAAACGCCTCCATTTGAAGGAAC
ATTTAGTCCTTGATGGATCTGGAAATGTTTTCAAATTAGCTGCACCAGTGGAGTGCAAGG
GCATCGTTGGTGGCGATGACAGGCATTATCTTCTTGATTTGTTGAGGGCAACTCCTCGGG
ATGCCAACTATAGTGGACCTGGTTCTCGATTTTGTATCTTGAGACAAGAATTAATTACTG
CCTTTTGTCAGGTCCAAGCAGCTGAGACAACCAAATCTGAGGAGACGAGTACTCAAGGTG
CAGATAATTTGGCTACTGATTCTCAAAATGGCATTGACGCTGACAAGCCGGATTTAACAG
TAGAAGAAAAAGCGGAGGATGCGAAAGGACATGCTTCTGCATCTACTGAAACCTCTGGTT
GCAAAGACGAAATTACTTTCAATCCTAATGTTTTCACTGGATTCAAACTTGCTGGGAGTC
CAGAGGAAATTGGAGCTGATGAAGCAAATGTGAGAAAAGTTAGTCAATATCTTACTGACG
TCGTACTTCCGAAGTTTGTACAAG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000105A_C01 KMC000105A_c01
         (684 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_190782.1| putative protein; protein id: At3g52140.1 [Arab...   272  4e-72
sp|O15818|IF3X_DICDI PUTATIVE EUKARYOTIC TRANSLATION INITIATION ...   102  4e-21
ref|XP_220693.1| similar to mKIAA0664 protein [Mus musculus] [Ra...   100  2e-20
gb|EAA00110.1| agCP9304 [Anopheles gambiae str. PEST]                  98  1e-19
ref|NP_056044.1| KIAA0664 protein [Homo sapiens] gi|10434718|dbj...    97  2e-19

>ref|NP_190782.1| putative protein; protein id: At3g52140.1 [Arabidopsis thaliana]
            gi|11357771|pir||T49093 hypothetical protein F4F15.250 -
            Arabidopsis thaliana gi|4678943|emb|CAB41334.1| putative
            protein [Arabidopsis thaliana]
          Length = 1403

 Score =  272 bits (695), Expect = 4e-72
 Identities = 137/230 (59%), Positives = 168/230 (72%), Gaps = 14/230 (6%)
 Frame = +3

Query: 36   SEVLPGILQGDKSDSLLYGSVDNGKKICWNEDFHSKVSEAAKRLHLKEHLVLDGSGNVFK 215
            S VLPGILQGDKSD+LLYGSVDNGKKICWNEDFH+KV EAAK LH+KEH V+D S  VFK
Sbjct: 606  SSVLPGILQGDKSDALLYGSVDNGKKICWNEDFHAKVLEAAKLLHIKEHSVIDASETVFK 665

Query: 216  LAAPVECKGIVGGDDRHYLLDLLRATPRDANYSGPGSRFCILRQELITAFCQVQAAETTK 395
            LAAPVECKGIVG D+RHYLLDL+R TPRDANY+GP SRFC+LR ELIT+FCQ ++ E +K
Sbjct: 666  LAAPVECKGIVGSDNRHYLLDLMRVTPRDANYTGPESRFCVLRPELITSFCQAESLEKSK 725

Query: 396  SEETSTQGAD---NLATDSQ-----------NGIDADKPDLTVEEKAEDAKGHASASTET 533
             +  + +G D   N++ D+            NG          +++   A+ +A+ S+E+
Sbjct: 726  FKTKADEGGDDSSNVSADTSKVGDALIDGEANGASNSDQKSISDKQNTTAEDYAAGSSES 785

Query: 534  SGCKDEITFNPNVFTGFKLAGSPEEIGADEANVRKVSQYLTDVVLPKFVQ 683
            S   D+I FNPNVFT F L G+ EEI ADE NV+KVS YL DVVLPKF++
Sbjct: 786  SKSCDQIAFNPNVFTDFTLGGNQEEIAADEENVKKVSSYLVDVVLPKFIE 835

>sp|O15818|IF3X_DICDI PUTATIVE EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT (EIF-3)
            gi|2281117|gb|AAC26527.1| 150-kD protein [Dictyostelium
            discoideum]
          Length = 1321

 Score =  102 bits (255), Expect = 4e-21
 Identities = 67/239 (28%), Positives = 115/239 (48%), Gaps = 22/239 (9%)
 Frame = +3

Query: 33   SSEVLPGILQGDKSDSLLYGSVD---------------------NGKKICWNEDFHSKVS 149
            +  ++PGIL  +K+  + YGS+D                     N K I  + +FHS++ 
Sbjct: 535  AQSLIPGILTTEKTSKIYYGSMDTPTNEEEEQQQKEENEENKNNNTKSIKADPEFHSRLL 594

Query: 150  EAAKRLHLKEHLVLDGSGNV-FKLAAPVECKGIVGGDDRHYLLDLLRATPRDANYSGPGS 326
            +AA  LHL E  V+    N    +    E KGI+G D R Y+LDL++ATPRD NY+    
Sbjct: 595  QAASLLHLSESKVISEDTNQEVSVCTSFESKGIIGIDGRRYILDLIKATPRDPNYTETKD 654

Query: 327  RFCILRQELITAFCQVQAAETTKSEETSTQGADNLATDSQNGIDADKPDLTVEEKAEDAK 506
            +  +LR E I  + +     T  +++   +  +      + GID      T   + ED +
Sbjct: 655  QLSVLRPEAIATYSEYFKV-TWLNQKRQQKLKEKEERQKKEGIDPP----TATARDEDVQ 709

Query: 507  GHASASTETSGCKDEITFNPNVFTGFKLAGSPEEIGADEANVRKVSQYLTDVVLPKFVQ 683
                 + E       ++FNPN+F+  KL G+PEE   D  +++ +  +L  +++P+ ++
Sbjct: 710  ----LTEEDLAQSPVVSFNPNLFSKVKLGGTPEEQQKDIEDLKAIGAFLKGILIPRLIE 764

>ref|XP_220693.1| similar to mKIAA0664 protein [Mus musculus] [Rattus norvegicus]
          Length = 1310

 Score =  100 bits (249), Expect = 2e-20
 Identities = 68/227 (29%), Positives = 106/227 (45%), Gaps = 18/227 (7%)
 Frame = +3

Query: 6    YNDYRVTECSSEVLPGILQGDKSDSLLYGSVDNGKKICWNEDFHSKVSEAAKRLHLKEHL 185
            Y  YRVT  +  ++PGIL+ D+  S++YGS+D GK +  +  +   +   ++ L +  H 
Sbjct: 457  YRGYRVT--AQSIIPGILERDQEQSVIYGSIDFGKTVVSHPRYLELLERTSRPLKILRHR 514

Query: 186  VLDGSGNVFKLAAPVECKGIVGGDDRHYLLDLLRATPRDANY-SGPGS------------ 326
            VL+      +L + VECKGI+G D RHY+LDLLR  P D N+   PG             
Sbjct: 515  VLNDRDEEVELCSSVECKGIIGNDGRHYILDLLRTFPPDLNFLPVPGEELPEECSRAGFP 574

Query: 327  -----RFCILRQELITAFCQVQAAETTKSEETSTQGADNLATDSQNGIDADKPDLTVEEK 491
                 + C LRQEL+ AF + +     K              D+   ++   P  + E K
Sbjct: 575  RTHRHKLCCLRQELVDAFVEHRYLLFMKLAALQLMQQKASKVDTSTSLENGGPPSSTETK 634

Query: 492  AEDAKGHASASTETSGCKDEITFNPNVFTGFKLAGSPEEIGADEANV 632
            +ED+ G        +GC++E     +V    K+    E I +D+  V
Sbjct: 635  SEDSLG------SEAGCEEE---GSSVSGLAKVKELAETIASDDGTV 672

>gb|EAA00110.1| agCP9304 [Anopheles gambiae str. PEST]
          Length = 1482

 Score = 97.8 bits (242), Expect = 1e-19
 Identities = 67/215 (31%), Positives = 98/215 (45%), Gaps = 33/215 (15%)
 Frame = +3

Query: 6    YNDYRVTECSSEVLPGILQGDKSDSLLYGSVDNGKKICWNEDFHSKVSEAAKRLHLKEHL 185
            Y  YRVT  +  ++PGIL+ ++  S++YGS+D GK +  +  +   ++ A K L +  H 
Sbjct: 549  YRGYRVT--AQSIIPGILEREQDQSVVYGSIDFGKTVLSHPKYLELLNAAGKHLKILPHS 606

Query: 186  VLDGSGNVFKLAAPVECKGIVGGDDRHYLLDLLRATPRDANY---------SGPGSR--- 329
            V +      +L + VECKGI+G D RHY+LDLLR  P D N+          G  SR   
Sbjct: 607  VYNDKEEAIELCSSVECKGIIGNDGRHYILDLLRTFPPDVNFLALPAEEEAVGKESRAMG 666

Query: 330  --------FCILRQELITAF-------------CQVQAAETTKSEETSTQGADNLATDSQ 446
                     C LRQEL+ AF              Q+Q     K E+ +           Q
Sbjct: 667  FPIEHRHKLCCLRQELLEAFVENRYLMFMKHAAVQLQQCVKMKQEQKAAAAQKTEEGGKQ 726

Query: 447  NGIDADKPDLTVEEKAEDAKGHASASTETSGCKDE 551
              I+A  P    +  A+DAK    A  + +  K+E
Sbjct: 727  AAIEAAAPAEGDKTPAKDAKDGKEAGKDANDGKEE 761

>ref|NP_056044.1| KIAA0664 protein [Homo sapiens] gi|10434718|dbj|BAB14354.1| unnamed
           protein product [Homo sapiens]
          Length = 720

 Score = 97.4 bits (241), Expect = 2e-19
 Identities = 66/197 (33%), Positives = 99/197 (49%), Gaps = 22/197 (11%)
 Frame = +3

Query: 6   YNDYRVTECSSEVLPGILQGDKSDSLLYGSVDNGKKICWNEDFHSKVSEAAKRLHLKEHL 185
           Y  YRVT  +  ++PGIL+ D+  S++YGS+D GK +  +  +   +   ++ L +  H 
Sbjct: 80  YRGYRVT--AQSIIPGILERDQEQSVIYGSIDFGKTVVSHPRYLELLERTSRPLKILRHQ 137

Query: 186 VLDGSGNVFKLAAPVECKGIVGGDDRHYLLDLLRATPRDANY-SGPGS------------ 326
           VL+      +L + VECKGI+G D RHY+LDLLR  P D N+   PG             
Sbjct: 138 VLNDRDEEVELCSSVECKGIIGNDGRHYILDLLRTFPPDLNFLPVPGEELPEECARAGFP 197

Query: 327 -----RFCILRQELITAFCQVQAAETTK--SEETSTQGADNLATDS--QNGIDADKPDLT 479
                + C LRQEL+ AF + +     K  + +   Q A  L T S  +NG  +     +
Sbjct: 198 RAHRHKLCCLRQELVDAFVEHRYLLFMKLAALQLMQQNASQLETPSSLENGGPS-----S 252

Query: 480 VEEKAEDAKGHASASTE 530
           +E K+ED  G  + S E
Sbjct: 253 LESKSEDPPGQEAGSEE 269

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 566,529,852
Number of Sequences: 1393205
Number of extensions: 12156572
Number of successful extensions: 32597
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 31353
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32545
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 30552968016
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GENLf071f05 BP066193 1 530 2 MFBL038c01_f BP043166 159 583 3 GENLf004b06 BP062554 176 684

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