Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC000069A_C04 KMC000069A_c04
(1186 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAA89019.1| cobalamine-independent methionine synthase [Sole... 303 e-120
sp|Q42662|METE_SOLSC 5-methyltetrahydropteroyltriglutamate--homo... 303 e-120
gb|AAF74983.1|AF082893_1 methionine synthase [Solanum tuberosum] 303 e-120
ref|NP_187028.1| putative methionine synthase; protein id: At3g0... 298 e-118
sp|P93263|METE_MESCR 5-methyltetrahydropteroyltriglutamate--homo... 298 e-118
>emb|CAA89019.1| cobalamine-independent methionine synthase [Solenostemon
scutellarioides]
Length = 784
Score = 303 bits (777), Expect(2) = e-120
Identities = 161/212 (75%), Positives = 173/212 (80%), Gaps = 4/212 (1%)
Frame = -1
Query: 949 LTKRPMKGMLTGPVTILNWSLVRNDQPRSETTYQIALAIKDEVEDLEKGGINVIQMDEAA 770
+T+RPMKGMLTGPVTILNWS VRNDQPR ET YQIALAIKDEVEDLEK GI VIQ+DEAA
Sbjct: 568 MTQRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAA 627
Query: 769 LREGLPLRKSEQAHYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVI 590
LREGLPLRKSE A YLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSII+MDADVI
Sbjct: 628 LREGLPLRKSEHAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIINMDADVI 687
Query: 589 TIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPKNPTY**NC*QDQQD----ACSA*E 422
TIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSP+ P+ ++ D + E
Sbjct: 688 TIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPST-----EEIADRINKMLAVLE 742
Query: 421 ERLVGEPLTVVSRPVSTAEVKPALTNMVAAAK 326
++ + AEVKPAL NMVAAAK
Sbjct: 743 TNILWVNPDCGLKTRKYAEVKPALENMVAAAK 774
Score = 151 bits (381), Expect(2) = e-120
Identities = 70/75 (93%), Positives = 73/75 (97%)
Frame = -2
Query: 1170 VELQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSR 991
V+LQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVSR
Sbjct: 494 VKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSR 553
Query: 990 PKPMTVFWSSLAQSL 946
PKPMTVFWS+ AQS+
Sbjct: 554 PKPMTVFWSTAAQSM 568
Score = 56.2 bits (134), Expect(2) = 4e-09
Identities = 25/29 (86%), Positives = 27/29 (92%)
Frame = -2
Query: 486 PRIPPTDEIADRINKMLAVLEKNVLWVNP 400
PRIP T+EIADRINKMLAVLE N+LWVNP
Sbjct: 722 PRIPSTEEIADRINKMLAVLETNILWVNP 750
Score = 27.7 bits (60), Expect(2) = 4e-09
Identities = 10/11 (90%), Positives = 11/11 (99%)
Frame = -3
Query: 401 PDCGLKTRKYS 369
PDCGLKTRKY+
Sbjct: 750 PDCGLKTRKYA 760
>sp|Q42662|METE_SOLSC 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
(Vitamin-B12-independent methionine synthase isozyme)
(Cobalamin-independent methionine synthase isozyme)
Length = 764
Score = 303 bits (777), Expect(2) = e-120
Identities = 161/212 (75%), Positives = 173/212 (80%), Gaps = 4/212 (1%)
Frame = -1
Query: 949 LTKRPMKGMLTGPVTILNWSLVRNDQPRSETTYQIALAIKDEVEDLEKGGINVIQMDEAA 770
+T+RPMKGMLTGPVTILNWS VRNDQPR ET YQIALAIKDEVEDLEK GI VIQ+DEAA
Sbjct: 548 MTQRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAA 607
Query: 769 LREGLPLRKSEQAHYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVI 590
LREGLPLRKSE A YLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSII+MDADVI
Sbjct: 608 LREGLPLRKSEHAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIINMDADVI 667
Query: 589 TIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPKNPTY**NC*QDQQD----ACSA*E 422
TIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSP+ P+ ++ D + E
Sbjct: 668 TIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPST-----EEIADRINKMLAVLE 722
Query: 421 ERLVGEPLTVVSRPVSTAEVKPALTNMVAAAK 326
++ + AEVKPAL NMVAAAK
Sbjct: 723 TNILWVNPDCGLKTRKYAEVKPALENMVAAAK 754
Score = 151 bits (381), Expect(2) = e-120
Identities = 70/75 (93%), Positives = 73/75 (97%)
Frame = -2
Query: 1170 VELQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSR 991
V+LQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVSR
Sbjct: 474 VKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSR 533
Query: 990 PKPMTVFWSSLAQSL 946
PKPMTVFWS+ AQS+
Sbjct: 534 PKPMTVFWSTAAQSM 548
Score = 56.2 bits (134), Expect(2) = 4e-09
Identities = 25/29 (86%), Positives = 27/29 (92%)
Frame = -2
Query: 486 PRIPPTDEIADRINKMLAVLEKNVLWVNP 400
PRIP T+EIADRINKMLAVLE N+LWVNP
Sbjct: 702 PRIPSTEEIADRINKMLAVLETNILWVNP 730
Score = 27.7 bits (60), Expect(2) = 4e-09
Identities = 10/11 (90%), Positives = 11/11 (99%)
Frame = -3
Query: 401 PDCGLKTRKYS 369
PDCGLKTRKY+
Sbjct: 730 PDCGLKTRKYA 740
>gb|AAF74983.1|AF082893_1 methionine synthase [Solanum tuberosum]
Length = 765
Score = 303 bits (777), Expect(2) = e-120
Identities = 161/222 (72%), Positives = 175/222 (78%), Gaps = 12/222 (5%)
Frame = -1
Query: 952 ELTKRPMKGMLTGPVTILNWSLVRNDQPRSETTYQIALAIKDEVEDLEKGGINVIQMDEA 773
E+TKRPMKGMLTGPVTILNWS VRNDQPR ET YQIALAIKDEVEDLEK GI VIQ+DEA
Sbjct: 548 EMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEA 607
Query: 772 ALREGLPLRKSEQAHYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADV 593
ALREGLPLRK+E A YL+WAVHSFRITNVG++DTTQIHTHMCYSNFNDIIHSIIDMDADV
Sbjct: 608 ALREGLPLRKAEHAFYLNWAVHSFRITNVGIEDTTQIHTHMCYSNFNDIIHSIIDMDADV 667
Query: 592 ITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPKNPTY**NC*QDQQDACSA*EERL 413
ITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSP+ P+ ++ D
Sbjct: 668 ITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPST-----EEIADR-------- 714
Query: 412 VGEPLTVVSRPV------------STAEVKPALTNMVAAAKT 323
V + L V+ + EVKPAL NMV+AAKT
Sbjct: 715 VNKMLAVLDTNILWVNPDCGLKTRKYTEVKPALQNMVSAAKT 756
Score = 150 bits (380), Expect(2) = e-120
Identities = 70/75 (93%), Positives = 72/75 (95%)
Frame = -2
Query: 1170 VELQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSR 991
V+LQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVSR
Sbjct: 475 VDLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSR 534
Query: 990 PKPMTVFWSSLAQSL 946
PKPMTVFWSS AQ +
Sbjct: 535 PKPMTVFWSSKAQEM 549
Score = 54.7 bits (130), Expect(2) = 1e-08
Identities = 23/29 (79%), Positives = 27/29 (92%)
Frame = -2
Query: 486 PRIPPTDEIADRINKMLAVLEKNVLWVNP 400
PRIP T+EIADR+NKMLAVL+ N+LWVNP
Sbjct: 703 PRIPSTEEIADRVNKMLAVLDTNILWVNP 731
Score = 27.7 bits (60), Expect(2) = 1e-08
Identities = 10/11 (90%), Positives = 11/11 (99%)
Frame = -3
Query: 401 PDCGLKTRKYS 369
PDCGLKTRKY+
Sbjct: 731 PDCGLKTRKYT 741
>ref|NP_187028.1| putative methionine synthase; protein id: At3g03780.2, supported by
cDNA: 111720., supported by cDNA: gi_14532771
[Arabidopsis thaliana]
gi|6006863|gb|AAF00639.1|AC009540_16 putative methionine
synthase [Arabidopsis thaliana]
gi|21536794|gb|AAM61126.1| putative methionine synthase
[Arabidopsis thaliana] gi|23297275|gb|AAN12930.1|
putative methionine synthase [Arabidopsis thaliana]
Length = 765
Score = 298 bits (763), Expect(2) = e-118
Identities = 157/213 (73%), Positives = 170/213 (79%), Gaps = 5/213 (2%)
Frame = -1
Query: 949 LTKRPMKGMLTGPVTILNWSLVRNDQPRSETTYQIALAIKDEVEDLEKGGINVIQMDEAA 770
+TKRPMKGMLTGPVTILNWS VRNDQPR ET YQIALAIKDEVEDLEKGGI VIQ+DEAA
Sbjct: 549 MTKRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIGVIQIDEAA 608
Query: 769 LREGLPLRKSEQAHYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVI 590
LREGLPLRK+E + YLDWAVHSFRITN GVQD+TQIHTHMCYSNFNDIIHSIIDMDADVI
Sbjct: 609 LREGLPLRKAEHSFYLDWAVHSFRITNCGVQDSTQIHTHMCYSNFNDIIHSIIDMDADVI 668
Query: 589 TIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPKNPTY**NC*QDQ-----QDACSA* 425
TIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSP+ P+ D+ +
Sbjct: 669 TIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPS------TDEIADRINKMLAVL 722
Query: 424 EERLVGEPLTVVSRPVSTAEVKPALTNMVAAAK 326
E+ ++ + EVKPAL MV AAK
Sbjct: 723 EQNILWVNPDCGLKTRKYTEVKPALKAMVDAAK 755
Score = 150 bits (379), Expect(2) = e-118
Identities = 68/75 (90%), Positives = 73/75 (96%)
Frame = -2
Query: 1170 VELQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSR 991
V++QE+LDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPP+IYGDVSR
Sbjct: 475 VDIQEDLDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPVIYGDVSR 534
Query: 990 PKPMTVFWSSLAQSL 946
PKPMTVFWSS AQS+
Sbjct: 535 PKPMTVFWSSTAQSM 549
Score = 58.5 bits (140), Expect(2) = 9e-10
Identities = 26/29 (89%), Positives = 28/29 (95%)
Frame = -2
Query: 486 PRIPPTDEIADRINKMLAVLEKNVLWVNP 400
PRIP TDEIADRINKMLAVLE+N+LWVNP
Sbjct: 703 PRIPSTDEIADRINKMLAVLEQNILWVNP 731
Score = 27.7 bits (60), Expect(2) = 9e-10
Identities = 10/11 (90%), Positives = 11/11 (99%)
Frame = -3
Query: 401 PDCGLKTRKYS 369
PDCGLKTRKY+
Sbjct: 731 PDCGLKTRKYT 741
>sp|P93263|METE_MESCR 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
(Vitamin-B12-independent methionine synthase isozyme)
(Cobalamin-independent methionine synthase isozyme)
gi|7433427|pir||T12575
5-methyltetrahydropteroyltriglutamate-homocysteine
S-methyltransferase (EC 2.1.1.14) - common ice plant
gi|1814403|gb|AAB41896.1| methionine synthase
[Mesembryanthemum crystallinum]
Length = 765
Score = 298 bits (762), Expect(2) = e-118
Identities = 160/212 (75%), Positives = 168/212 (78%), Gaps = 4/212 (1%)
Frame = -1
Query: 949 LTKRPMKGMLTGPVTILNWSLVRNDQPRSETTYQIALAIKDEVEDLEKGGINVIQMDEAA 770
+T RPMKGMLTGPVTILNWS VRNDQPR ET YQIALAIK+EVEDLEK GINVIQ+DEAA
Sbjct: 549 MTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKNEVEDLEKAGINVIQIDEAA 608
Query: 769 LREGLPLRKSEQAHYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVI 590
LREGLPLRKSE YL WAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVI
Sbjct: 609 LREGLPLRKSEHDFYLKWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVI 668
Query: 589 TIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPKNPTY**NC*QDQQDACSA*EERLV 410
TIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSP+ P ++ D L
Sbjct: 669 TIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPPT-----EELADRIRKMLAVLE 723
Query: 409 GEPLTVVS----RPVSTAEVKPALTNMVAAAK 326
L V + EV PAL+NMVAAAK
Sbjct: 724 SNVLWVNPDCGLKTRKYGEVNPALSNMVAAAK 755
Score = 150 bits (378), Expect(2) = e-118
Identities = 69/75 (92%), Positives = 73/75 (97%)
Frame = -2
Query: 1170 VELQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSR 991
V+LQEELDIDVLVHGEPERNDMVEYFGEQLSGFAF+ NGWVQSYGSRCVKPPIIYGDVSR
Sbjct: 475 VKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFSANGWVQSYGSRCVKPPIIYGDVSR 534
Query: 990 PKPMTVFWSSLAQSL 946
P PMTVFWSS+AQS+
Sbjct: 535 PNPMTVFWSSMAQSM 549
Score = 57.0 bits (136), Expect(2) = 7e-10
Identities = 25/29 (86%), Positives = 27/29 (92%)
Frame = -2
Query: 486 PRIPPTDEIADRINKMLAVLEKNVLWVNP 400
PRIPPT+E+ADRI KMLAVLE NVLWVNP
Sbjct: 703 PRIPPTEELADRIRKMLAVLESNVLWVNP 731
Score = 29.6 bits (65), Expect(2) = 7e-10
Identities = 13/17 (76%), Positives = 14/17 (81%)
Frame = -3
Query: 401 PDCGLKTRKYSRGEASP 351
PDCGLKTRKY GE +P
Sbjct: 731 PDCGLKTRKY--GEVNP 745
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,140,183,596
Number of Sequences: 1393205
Number of extensions: 28054510
Number of successful extensions: 92088
Number of sequences better than 10.0: 149
Number of HSP's better than 10.0 without gapping: 83443
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 91485
length of database: 448,689,247
effective HSP length: 125
effective length of database: 274,538,622
effective search space used: 73850889318
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
