Supplemental Data for the Publication

Asamizu E, Nakamura Y, Miura K, Fukuzawa H, Fujiwara S, Hirono M, Iwamoto K, Matsuda Y, Minagawa J, Shimogawara K, Takahashi Y, Tabata S
Establishment of Publicly Available cDNA Material and Information Resource of Chlamydomonas reinhardtii (Chlorophyta), to Facilitate Gene Function Analysis
Phycologia (2004) In press

Supplemental Data for the Publication

Table 1. Complete list of growth conditions of cells used in construction of the cDNA library

Kinds of conditions Before induction Growth conditions

Heterotrophic growth HS^a) (no acetate) TAP^b) (acetate) for 30 min and 300 min

Autotrophic growth TAP (acetate) HS (no acetate) for 30 min

Dark to light TAP (dark) TAP (150μEm^-1s^-1) for 30 min

Light to dark TAP (150μEm^-1s^-1) TAP (dark) for 30 min and 300 min

High light to dark HS (600μEm^-1s^-1) HS (dark) for 30 min

High light HS (150μEm^-1s^-1) HS (600μEm^-1s^-1) for 30 min

High light TAP (10μE/m²/s) High light (1000μE/m²/s ) in TAP for 0, 1, 2 hours

High light TAP (10μE/m²/s) High light (2000μE/m²/s ) in TAP for 1 hour

High light TAP (10μE/m²/s) High light (2000μE/m²/s ) in TAP for 2 hours

High light steady-state TAP (300μEm^-1s^-1, 5% CO₂) TAP (300μEm^-1s^-1, 5% CO₂)

Dark steady-state TAP (dark, no aeration) TAP (dark, no aeration)

High CO₂ TAP (0.04% CO₂) HS (3% CO₂) for 24 hr , 52 hr and 72 hr

High CO₂ TAP (0.04% CO₂) HS (10% CO₂) for 26 hr and 53 hr

High CO₂ HS (0.04% CO₂) HS (5% CO₂) for 30 min

Super high CO₂ HS (5% CO₂) HS (100% CO₂) for 30 min

Low CO₂ HS (5% CO₂) HS (0.04% CO₂) for 30 min

No CO₂ HS (5% CO₂) HS (100% N₂) for 30 min

N-starvation HS (+NH₄) HS (-NH₄) for 5 hr

K-starvation HS (+K) HS (-K) for 5 hr

Fe-starvation HS (+Fe) HS (-Fe) for 5 hr

Cu-starvation HS (+Cu) HS (-Cu) for 5 hr

Ca-starvation HS (+Ca) HS (-Ca) for 5 hr

P-starvation TAP(+Pi) TAP(-Pi) for 12 hr

Heat stress TAP (25℃) for 2 hr TAP (38℃) for 1 and 2 hr

Heat stress HS (28℃) HS (42℃) for 5 min and 30 min

Cold stress HS (28℃) HS (10℃) for 30 min

Remediatioin HS (28℃) to HS (10℃) for 2 hr HS (28℃) for 30 min

Salt stress 3/10 x HS 2 x HS

Hypotonic stress HS Water

Alkaline stress HS (pH 6.8) HS (pH7.5)

Acidic stress HS (pH 6.8) HS (pH5.5)

Arsenate stress TAP TAP (+1 mM arsenate) for 6 hr

Divalent metal-limiting HS (-EDTA) HS (+1.0 mM ESTA)

Deflagellation TAP Deflagellated by pH shock^c)

Gametogenesis Synchronous culture in M^d) M-N for 8 h (sampled every 1 hr)

Dedifferentiation (gamete to vegetative) Gametes in M-N +N for 8 h (sampled for every 1 hr)

Zygote formation Gametes in M-N Zygotes at 0.5, 1, 1.5, 2, 4 and 6 hr after mixing the mt+ and mt- gametes

Cell cycle 12 hr light- 12 hr dark in M 24 hr-cell cycle (sampled every 2 hr)

a) Sueoka 1960, b) Gorman and Levinev 1965, c) Witman 1986, d) Matsuda et al. 1971

Table 2. Complete list of GO categories of C. reinhardtii consensus or singleton EST sequences

G0 category Number of consensus or singleton ESTs

1,4-alpha-glucan branching enzyme activity 4

12-oxophytodienoate reductase activity 2

1-deoxy-D-xylulose-5-phosphate reductoisomerase activity 1

1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase activity 4

1-phosphatidylinositol-4-phosphate 5-kinase activity 2

2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity 3

2-isopropylmalate synthase activity 1

3' tRNA processing endoribonuclease activity 1

3-deoxy-7-phosphoheptulonate synthase activity 1

3-hydroxybutyryl-CoA dehydrogenase activity 2

3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity 2

3-oxo-5-alpha-steroid 4-dehydrogenase activity 2

4-alpha-glucanotransferase activity 1

4-diphosphocytidyl-2C-methyl-D-erythritol kinase activity 1

4-hydroxyphenylpyruvate dioxygenase activity 2

5'-3' exoribonuclease activity 2

8-amino-7-oxononanoate synthase activity 1

acetate-CoA ligase activity 5

acetolactate synthase activity 5

acetyl-CoA carboxylase activity 2

acid phosphatase activity 1

acid-D-amino acid ligase activity 1

aconitate hydratase activity 2

actin binding 90

acyl carrier activity 2

acyl-[acyl-carrier protein] desaturase activity 1

acyl-CoA binding 2

acyl-CoA oxidase activity 2

acyltransferase activity 4

adenosine kinase activity 1

adenosylmethionine decarboxylase activity 1

adenylate kinase activity 2

adenylosuccinate lyase activity 2

adenylyl-sulfate reductase activity 1

aging 2

alanine-glyoxylate transaminase activity 2

alanyl-tRNA aminoacylation 4

aldehyde dehydrogenase activity 8

aldo-keto reductase activity 5

alpha1-adrenergic receptor activity 1

amine oxidase activity 1

amino acid binding 2

amino acid metabolism 1

amino acid permease activity 1

aminoacylase activity 1

aminomethyltransferase activity 1

AMP binding 2

antioxidant activity 7

ARF small monomeric GTPase activity 6

arginine biosynthesis 3

argininosuccinate synthase activity 2

arginyl-tRNA aminoacylation 3

aromatic amino acid family biosynthesis 1

aromatic compound metabolism 1

aryl-alcohol dehydrogenase activity 2

asparagine biosynthesis 1

asparagine synthase (glutamine-hydrolyzing) activity 1

aspartate kinase activity 2

aspartate transaminase activity 1

aspartyl aminopeptidase activity 2

aspartyl-tRNA aminoacylation 2

ATP binding 37

ATP dependent DNA helicase activity 1

ATP dependent helicase activity 29

ATP phosphoribosyltransferase activity 1

ATP synthesis coupled proton transport 1

ATP:ADP antiporter activity 2

ATPase activity 25

ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism 4

ATP-binding cassette (ABC) transporter activity 30

ATP-dependent proteolysis 1

autophagy 1

auxin polar transport 2

beta-amylase activity 2

binding 2

biosynthesis 7

branched chain family amino acid biosynthesis 1

brassinosteroid biosynthesis 1

C-5 sterol desaturase activity 1

C-8 sterol isomerase activity 1

calcium ion binding 9

calcium ion sensing 2

calcium ion storage activity 1

calcium-dependent phospholipid binding 1

calcium-mediated signaling 1

calmodulin binding 10

carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity 2

carbohydrate binding 1

carbohydrate metabolism 9

carbon utilization 1

carboxy-lyase activity 2

carboxypeptidase A activity 1

carotene 7,8-desaturase activity 1

carotene biosynthesis 3

carrier activity 1

caspase activity 1

catalytic activity 24

cation transport 1

cationic amino acid transporter activity 2

cation-transporting ATPase activity 2

CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity 1

cell adhesion 2

cell adhesion receptor activity 1

cell cycle 6

cell elongation 2

cell growth and/or maintenance 8

cell plate 2

cell surface 1

cell surface receptor linked signal transduction 1

cell wall (sensu Magnoliophyta) 2

cellular morphogenesis during differentiation 2

cellular response to glucose starvation 1

cellular_component unknown 33

cellulose biosynthesis 1

chalcone isomerase activity 1

chaperone activity 13

chlorophyll binding 1

chlorophyll catabolism 1

chlorophyll synthetase activity 2

chloroplast 116

chloroplast envelope 6

chloroplast inner membrane 5

chloroplast stroma 12

chloroplast stromal thylakoid 2

chromatin 1

chromosome organization and biogenesis (sensu Eukarya) 1

cinnamyl-alcohol dehydrogenase activity 3

citrate (pro-3S)-lyase activity 2

clathrin binding 8

clathrin vesicle coat 5

cleavage/polyadenylation specificity factor activity 1

COPI vesicle coat 5

copper chaperone activity 1

copper ion binding 3

coproporphyrinogen oxidase activity 1

cortical microtubule (sensu Viridiplantae) 2

C-terminal protein amino acid methylation 1

cuticle biosynthesis 1

cyclin binding 1

cyclin-dependent protein kinase activity 2

cyclin-dependent protein kinase regulator activity 2

cystathionine beta-lyase activity 1

cysteine biosynthesis 1

cysteine protease inhibitor activity 1

cysteine synthase activity 1

cysteine-type peptidase activity 7

cysteinyl-tRNA aminoacylation 1

cytochrome biogenesis 3

cytokinesis 1

cytokinesis by cell plate formation 1

cytoplasm 9

cytoskeleton 44

cytoskeleton organization and biogenesis 1

cytosol 2

cytosolic large ribosomal subunit (sensu Bacteria) 6

damaged DNA binding 1

de novo' IMP biosynthesis 1

defense response 1

defense response to bacteria 2

defense/immunity protein activity 1

delta1-pyrroline-5-carboxylate synthetase activity 1

deoxyribonuclease activity 1

development 3

diacylglycerol kinase activity 1

dihydrolipoyllysine-residue acetyltransferase activity 4

dihydropyrimidinase activity 1

disulfide oxidoreductase activity 2

DNA binding 121

DNA dependent ATPase activity 8

DNA endoreduplication 1

DNA helicase activity 2

DNA methylation 7

DNA polymerase processivity factor activity 1

DNA recombination 3

DNA repair 2

DNA replication 7

DNA replication, priming 1

DNA unwinding 1

DNA-directed DNA polymerase activity 2

DNA-directed RNA polymerase III complex 1

double-stranded DNA binding 1

drug transporter activity 1

dTDP-glucose 4,6-dehydratase activity 3

electron carrier activity 5

electron transport 42

electron transporter activity 1

electron transporter, transferring electrons within CoQH2-cytochrome c reductase complex activity 1

embryonic development 3

embryonic development (sensu Magnoliophyta) 1

endomembrane system 82

endoplasmic reticulum 3

endoplasmic reticulum membrane 2

endosome membrane 1

endosperm development 2

enoyl-CoA hydratase activity 3

extracellular 11

farnesyltranstransferase activity 1

fatty acid beta-oxidation 1

fatty acid biosynthesis 4

fatty-acid synthase activity 2

ferredoxin reductase activity 1

ferredoxin:thioredoxin reductase activity 1

ferric iron binding 2

FK506 binding 2

flower development 3

FMN binding 1

folic acid and derivative biosynthesis 3

formaldehyde dehydrogenase (glutathione) activity 2

fructose metabolism 1

fructose-bisphosphatase activity 1

galactolipid galactosyltransferase activity 1

galactose biosynthesis 1

galactose metabolism 4

gamma-glutamyl hydrolase activity 1

GDP-mannose transporter activity 1

geranylgeranyl reductase activity 2

geranylgeranyl-diphosphate geranylgeranyltransferase activity 2

gluconeogenesis 1

glucose metabolism 1

glucose-1-phosphate adenylyltransferase activity 3

glutamate synthase (ferredoxin) activity 6

glutamate synthase (NADH) activity 2

glutamate-1-semialdehyde 2,1-aminomutase activity 1

glutamate-ammonia ligase activity 5

glutamine-tRNA ligase activity 1

glutamyl-tRNA aminoacylation 3

glutamyl-tRNA reductase activity 2

glutathione peroxidase activity 4

glutathione synthase activity 1

glutathione transferase activity 5

glutathione-disulfide reductase activity 1

glyceraldehyde-3-phosphate dehydrogenase activity 4

glycerol-3-phosphate dehydrogenase complex 2

glycerol-3-phosphate O-acyltransferase activity 1

glycine catabolism 1

glycine decarboxylation via glycine cleavage system 3

glycine transaminase activity 3

glycolysis 16

glycyl-tRNA aminoacylation 3

glyoxysome 1

glyphosate metabolism 1

GMP synthase (glutamine-hydrolyzing) activity 1

Golgi apparatus 1

Golgi stack 1

G-protein coupled receptor activity 4

G-protein coupled receptor protein signaling pathway 24

GTP binding 19

GTPase activity 1

guanosine tetraphosphate (5'-ppGpp-3') metabolism 2

guanyl-nucleotide exchange factor activity 1

heat shock protein activity 3

heme oxidation 1

heterotrimeric G-protein complex 1

high affinity iron ion transport 1

histidine biosynthesis 1

histidine phosphotransfer kinase activity 3

histidinol-phosphate transaminase activity 2

histone acetyltransferase activity 2

histone deacetylation 2

hydrogen peroxide catabolism 1

hydrogen-exporting ATPase activity, phosphorylative mechanism 1

hydrogen-translocating V-type ATPase activity 6

hydrogen-transporting ATP synthase activity, rotational mechanism 6

hydrogen-transporting ATPase activity, rotational mechanism 1

hydrolase activity 24

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds 1

hydrolase activity, acting on ester bonds 1

hydrolase activity, hydrolyzing O-glycosyl compounds 10

hydro-lyase activity 1

hydroxymethyl-, formyl- and related transferase activity 2

hypusine biosynthesis from peptidyl-lysine 1

immune response 2

inorganic anion transporter activity 1

inositol/phosphatidylinositol kinase activity 5

inositol/phosphatidylinositol phosphatase activity 1

integral to membrane 30

intracellular 5

intracellular protein transport 7

intracellular signaling cascade 1

intracellular transporter activity 1

iron-sulfur cluster assembly 1

isomerase activity 2

isopentenyl diphosphate biosynthesis, mevalonate independent 3

kinase activity 122

kinase activity 52

kinesin motor activity 11

lactoylglutathione lyase activity 1

large ribosomal subunit 13

L-ascorbate peroxidase activity 1

leaf morphogenesis 1

leucine biosynthesis 2

leucine catabolism 2

light-harvesting complex 23

lipid binding 13

lipid metabolism 5

lipid storage 3

lipoxygenase activity 1

L-serine metabolism 3

lyase activity 2

lycopene beta cyclase activity 2

lycopene epsilon cyclase activity 1

lysine biosynthesis via diaminopimelate 4

magnesium chelatase activity 3

magnesium protoporphyrin IX methyltransferase activity 1

malate dehydrogenase activity 4

malate transport 5

mannitol dehydrogenase activity 2

MAPKKK cascade 1

mature ribosome assembly 2

membrane 62

membrane fraction 3

membrane fusion 1

metabolism 35

metal ion binding 3

metal ion transport 1

metal ion transporter activity 4

metalloexopeptidase activity 4

metallopeptidase activity 3

methionine biosynthesis 3

methionine metabolism 1

methionyl aminopeptidase activity 3

methionyl-tRNA aminoacylation 1

methylcrotonoyl-CoA carboxylase activity 2

methyltransferase activity 5

microsome 1

microtubule binding 1

microtubule-based process 2

mitochondrial inner membrane 1

mitochondrial matrix 1

mitochondrial outer membrane translocase complex 1

mitochondrion 35

mitosis 1

molecular_function unknown 124

Mo-molybdopterin cofactor biosynthesis 6

motor activity 1

mRNA binding 1

mRNA catabolism, nonsense-mediated 1

mRNA polyadenylation 1

mRNA processing 39

mRNA splice site selection 1

mRNA splicing 9

myosin 1

NADH dehydrogenase (ubiquinone) activity 5

NADH dehydrogenase activity 5

negative regulation of flower development 1

negative regulation of photomorphogenesis 2

negative regulation of transcription 2

nicotinamide adenine dinucleotide biosynthesis 3

nitrate reductase activity 2

nitrogen metabolism 1

N-linked glycosylation 1

N-linked glycosylation via asparagine 2

nuclear matrix 1

nuclear membrane 2

nuclease activity 1

nucleic acid binding 16

nucleobase, nucleoside, nucleotide and nucleic acid metabolism 1

nucleobase, nucleoside, nucleotide and nucleic acid transport 1

nucleocytoplasmic transport 1

nucleolus 1

nucleoside metabolism 3

nucleosome assembly 2

nucleotide binding 29

nucleotide-sugar metabolism 3

nucleotidyltransferase activity 5

nucleus 56

nutrient reservoir activity 8

oligosaccharyl transferase activity 1

omega-3 fatty acid desaturase activity 2

omega-6 fatty acid desaturase activity 2

organic anion transporter activity 11

ornithine-oxo-acid transaminase activity 2

ossification 1

oxidoreductase activity 26

oxidoreductase activity, acting on NADH or NADPH 1

oxidoreductase activity, acting on NADH or NADPH, NAD or NADP as acceptor 3

oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor 2

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors 1

oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor 2

oxidoreductase activity, acting on sulfur group of donors, other acceptors 1

oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor 2

oxidoreductase activity, acting on the CH-CH group of donors 2

oxoglutarate dehydrogenase (succinyl-transferring) activity 2

oxygen binding 4

palmitoyl-(protein) hydrolase activity 1

pectinesterase inhibitor activity 8

peptidase activity 15

peptide deformylase activity 1

peptidyl-proline hydroxylation to 4-hydroxy-L-proline 9

peptidyl-prolyl cis-trans isomerase activity 1

peroxisomal membrane 7

peroxisome 5

phenylalanyl-tRNA aminoacylation 2

phenylpropanoid metabolism 2

phosphate transporter activity 2

phosphatidate cytidylyltransferase activity 1

phosphoadenylyl-sulfate reductase (thioredoxin) activity 1

phosphoenolpyruvate carboxylase activity 5

phosphogluconate dehydrogenase (decarboxylating) activity 3

phosphoglycolate phosphatase activity 1

phospholipase A2 activity 1

phospholipase C activity 1

phospholipid biosynthesis 2

phosphopyruvate hydratase activity 2

phosphoribosyl-AMP cyclohydrolase activity 1

phosphoribosylformylglycinamidine cyclo-ligase activity 2

phosphoric diester hydrolase activity 2

phosphoric ester hydrolase activity 1

phosphorylase kinase activity 1

phosphorylation 1

phosphoserine phosphatase activity 5

phosphotransferase activity, phosphate group as acceptor 1

photomorphogenesis 1

photosynthesis 5

photosynthetic electron transport 2

photosystem I reaction center 2

phototropism 1

phragmoplast 4

phycobilisome 2

plasma membrane 3

plastid 4

plastid ribosome 2

plastid stroma 2

plastoquinol-plastocyanin reductase activity 2

pollen tube growth 1

porphobilinogen synthase activity 1

potassium ion transport 2

potassium:hydrogen antiporter activity 1

prefoldin complex 1

pre-mRNA splicing factor activity 16

prenyltransferase activity 1

proteasome regulatory particle, base subcomplex (sensu Eukarya) 8

proteasome regulatory particle, lid subcomplex (sensu Eukarya) 6

protein amino acid dephosphorylation 2

protein amino acid phosphorylation 9

protein binding 83

protein binding, bridging 1

protein biosynthesis 71

protein degradation tagging activity 3

protein disulfide oxidoreductase activity 1

protein farnesyltransferase complex 2

protein folding 32

protein geranylgeranylation 1

protein kinase activity 9

protein kinase CK2 regulator activity 1

protein methyltransferase activity 1

protein phosphatase type 1 activity 1

protein phosphatase type 2A activity 2

protein phosphatase type 2A regulator activity 3

protein phosphatase type 2C activity 9

protein secretion 2

protein serine/threonine kinase activity 11

protein serine/threonine phosphatase activity 8

protein sumoylation 1

protein targeting 1

protein threonine/tyrosine kinase activity 1

protein translocase activity 5

protein transport 4

protein transporter activity 11

protein tyrosine/serine/threonine phosphatase activity 3

protein ubiquitination 3

protein-arginine N-methyltransferase activity 2

protein-histidine kinase activity 2

protein-methionine-S-oxide reductase activity 4

protein-tyrosine kinase activity 6

proteolysis and peptidolysis 38

protochlorophyllide reductase activity 4

proton transport 1

proton-transporting ATP synthase complex, coupling factor F(o) (sensu Bacteria) 1

pseudouridine synthase activity 1

purine base metabolism 2

purine nucleotide biosynthesis 3

purine ribonucleoside monophosphate biosynthesis 1

purine ribonucleotide biosynthesis 3

pyridine nucleotide biosynthesis 1

pyrimidine base metabolism 1

pyrophosphatase activity 2

pyruvate decarboxylase activity 1

pyruvate dehydrogenase (acetyl-transferring) activity 3

pyruvate, water dikinase activity 1

RAN protein binding 1

receptor activity 2

reductive pentose-phosphate cycle 1

regulation of flower development 1

regulation of meristem size 3

regulation of transcription, DNA-dependent 16

regulation of translational initiation 1

removal of superoxide radicals 3

response to abscisic acid stimulus 1

response to aluminum ion 1

response to ethylene stimulus 1

response to heat 7

response to pathogenic bacteria (incompatible interaction) 5

response to pathogenic fungi 7

response to stress 4

response to UV 4

retrograde (endosome to Golgi) transport 3

rhodopsin-like receptor activity 49

ribonuclease activity 2

ribonucleoside-diphosphate reductase activity 1

ribose-5-phosphate isomerase activity 2

ribosome 3

ribosome biogenesis 29

ribosome biogenesis and assembly 1

ribulose-bisphosphate carboxylase activity 6

RNA binding 55

RNA helicase activity 4

RNA methyltransferase activity 4

RNA modification 1

RNA processing 3

RNA-directed DNA polymerase activity 1

rRNA processing 1

S-adenosylmethionine biosynthesis 1

S-adenosylmethionine-dependent methyltransferase activity 2

salinity response 1

SCF ubiquitin ligase complex 1

selenium binding 1

serine-type endopeptidase activity 21

serine-type endopeptidase inhibitor activity 1

serine-type peptidase activity 4

seryl-tRNA aminoacylation 2

sex determination 1

shoot morphogenesis 1

signal transducer activity 1

signal transduction 7

signal-recognition-particle GTPase activity 3

single-stranded DNA binding 1

small ribosomal subunit 6

SNF1A/AMP-activated protein kinase activity 2

sodium ion transport 1

solute:sodium symporter activity 2

SOS response 1

sphingolipid biosynthesis 1

spliceosome complex 1

squalene monooxygenase activity 1

starch catabolism 3

steroid biosynthesis 5

steroid metabolism 2

sterol carrier activity 1

structural constituent of cell wall 59

structural constituent of cytoskeleton 3

structural constituent of ribosome 10

structural molecule activity 14

subtilase activity 2

sugar binding 1

sulfate transporter activity 1

sulfite reductase activity 2

terpenoid biosynthesis 2

thiamin biosynthesis 2

thioglucosidase binding activity 2

thiol-disulfide exchange intermediate activity 16

threonine synthase activity 2

threonyl-tRNA aminoacylation 4

thylakoid 2

thylakoid (sensu Viridiplantae) 3

thylakoid lumen (sensu Viridiplantae) 28

thylakoid membrane (sensu Viridiplantae) 4

thylakoid membrane organization and biogenesis 1

thymidylate kinase activity 1

transaldolase activity 2

transaminase activity 5

transcription 22

transcription coactivator activity 1

transcription factor activity 27

transcription regulator activity 3

transferase activity 2

transferase activity, transferring glycosyl groups 16

transferase activity, transferring phosphorus-containing groups 1

trans-Golgi network transport vesicle 1

transketolase activity 2

translation elongation factor activity 11

translation factor activity, nucleic acid binding 4

translation initiation factor activity 7

translational elongation 6

translational initiation 12

translational initiation 8

translational termination 2

transport 25

transporter activity 4

transposase activity 1

tricarboxylic acid cycle 2

triose phosphate transport 5

tRNA aminoacylation for protein translation 16

tRNA processing 1

tyrosine catabolism 1

ubiquinol-cytochrome-c reductase activity 2

ubiquitin 1

ubiquitin conjugating enzyme activity 14

ubiquitin cycle 1

ubiquitin-dependent protein catabolism 5

ubiquitin-protein ligase activity 1

ubiquitin-specific protease activity 3

UDP-glucuronate decarboxylase activity 1

UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity 1

UDPsulfoquinovose synthase activity 1

uracil salvage 1

uridine kinase activity 1

uridylate kinase activity 1

uroporphyrinogen decarboxylase activity 3

vacuolar membrane 1

vacuolar membrane (sensu Magnoliophyta) 5

vesicle-mediated transport 2

viral capsid 1

viral replication complex formation and maintenance 1

vision 7

vitamin B2 biosynthesis 1

voltage-dependent ion-selective channel activity 2

voltage-gated chloride channel activity 1

xanthine dehydrogenase activity 2

xenobiotic-transporting ATPase activity 2

X-Pro dipeptidase activity 1

zeaxanthin epoxidase activity 2

zinc ion binding 9

zinc ion transport 2

Chlamydomonas reinhardtii
Kazusa DNA Research Institute
Department of Plant Gene Research

Kinds of conditions	Before induction	Growth conditions
Heterotrophic growth	HS^a) (no acetate)	TAP^b) (acetate) for 30 min and 300 min
Autotrophic growth	TAP (acetate)	HS (no acetate) for 30 min
Dark to light	TAP (dark)	TAP (150μEm^-1s^-1) for 30 min
Light to dark	TAP (150μEm^-1s^-1)	TAP (dark) for 30 min and 300 min
High light to dark	HS (600μEm^-1s^-1)	HS (dark) for 30 min
High light	HS (150μEm^-1s^-1)	HS (600μEm^-1s^-1) for 30 min
High light	TAP (10μE/m²/s)	High light (1000μE/m²/s ) in TAP for 0, 1, 2 hours
High light	TAP (10μE/m²/s)	High light (2000μE/m²/s ) in TAP for 1 hour
High light	TAP (10μE/m²/s)	High light (2000μE/m²/s ) in TAP for 2 hours
High light steady-state	TAP (300μEm^-1s^-1, 5% CO₂)	TAP (300μEm^-1s^-1, 5% CO₂)
Dark steady-state	TAP (dark, no aeration)	TAP (dark, no aeration)
High CO₂	TAP (0.04% CO₂)	HS (3% CO₂) for 24 hr , 52 hr and 72 hr
High CO₂	TAP (0.04% CO₂)	HS (10% CO₂) for 26 hr and 53 hr
High CO₂	HS (0.04% CO₂)	HS (5% CO₂) for 30 min
Super high CO₂	HS (5% CO₂)	HS (100% CO₂) for 30 min
Low CO₂	HS (5% CO₂)	HS (0.04% CO₂) for 30 min
No CO₂	HS (5% CO₂)	HS (100% N₂) for 30 min
N-starvation	HS (+NH₄)	HS (-NH₄) for 5 hr
K-starvation	HS (+K)	HS (-K) for 5 hr
Fe-starvation	HS (+Fe)	HS (-Fe) for 5 hr
Cu-starvation	HS (+Cu)	HS (-Cu) for 5 hr
Ca-starvation	HS (+Ca)	HS (-Ca) for 5 hr
P-starvation	TAP(+Pi)	TAP(-Pi) for 12 hr
Heat stress	TAP (25℃) for 2 hr	TAP (38℃) for 1 and 2 hr
Heat stress	HS (28℃)	HS (42℃) for 5 min and 30 min
Cold stress	HS (28℃)	HS (10℃) for 30 min
Remediatioin	HS (28℃) to HS (10℃) for 2 hr	HS (28℃) for 30 min
Salt stress	3/10 x HS	2 x HS
Hypotonic stress	HS	Water
Alkaline stress	HS (pH 6.8)	HS (pH7.5)
Acidic stress	HS (pH 6.8)	HS (pH5.5)
Arsenate stress	TAP	TAP (+1 mM arsenate) for 6 hr
Divalent metal-limiting	HS (-EDTA)	HS (+1.0 mM ESTA)
Deflagellation	TAP	Deflagellated by pH shock^c)
Gametogenesis	Synchronous culture in M^d)	M-N for 8 h (sampled every 1 hr)
Dedifferentiation (gamete to vegetative)	Gametes in M-N	+N for 8 h (sampled for every 1 hr)
Zygote formation	Gametes in M-N	Zygotes at 0.5, 1, 1.5, 2, 4 and 6 hr after mixing the mt+ and mt- gametes
Cell cycle	12 hr light- 12 hr dark in M	24 hr-cell cycle (sampled every 2 hr)

G0 category	Number of consensus or singleton ESTs
1,4-alpha-glucan branching enzyme activity	4
12-oxophytodienoate reductase activity	2
1-deoxy-D-xylulose-5-phosphate reductoisomerase activity	1
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase activity	4
1-phosphatidylinositol-4-phosphate 5-kinase activity	2
2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity	3
2-isopropylmalate synthase activity	1
3' tRNA processing endoribonuclease activity	1
3-deoxy-7-phosphoheptulonate synthase activity	1
3-hydroxybutyryl-CoA dehydrogenase activity	2
3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity	2
3-oxo-5-alpha-steroid 4-dehydrogenase activity	2
4-alpha-glucanotransferase activity	1
4-diphosphocytidyl-2C-methyl-D-erythritol kinase activity	1
4-hydroxyphenylpyruvate dioxygenase activity	2
5'-3' exoribonuclease activity	2
8-amino-7-oxononanoate synthase activity	1
acetate-CoA ligase activity	5
acetolactate synthase activity	5
acetyl-CoA carboxylase activity	2
acid phosphatase activity	1
acid-D-amino acid ligase activity	1
aconitate hydratase activity	2
actin binding	90
acyl carrier activity	2
acyl-[acyl-carrier protein] desaturase activity	1
acyl-CoA binding	2
acyl-CoA oxidase activity	2
acyltransferase activity	4
adenosine kinase activity	1
adenosylmethionine decarboxylase activity	1
adenylate kinase activity	2
adenylosuccinate lyase activity	2
adenylyl-sulfate reductase activity	1
aging	2
alanine-glyoxylate transaminase activity	2
alanyl-tRNA aminoacylation	4
aldehyde dehydrogenase activity	8
aldo-keto reductase activity	5
alpha1-adrenergic receptor activity	1
amine oxidase activity	1
amino acid binding	2
amino acid metabolism	1
amino acid permease activity	1
aminoacylase activity	1
aminomethyltransferase activity	1
AMP binding	2
antioxidant activity	7
ARF small monomeric GTPase activity	6
arginine biosynthesis	3
argininosuccinate synthase activity	2
arginyl-tRNA aminoacylation	3
aromatic amino acid family biosynthesis	1
aromatic compound metabolism	1
aryl-alcohol dehydrogenase activity	2
asparagine biosynthesis	1
asparagine synthase (glutamine-hydrolyzing) activity	1
aspartate kinase activity	2
aspartate transaminase activity	1
aspartyl aminopeptidase activity	2
aspartyl-tRNA aminoacylation	2
ATP binding	37
ATP dependent DNA helicase activity	1
ATP dependent helicase activity	29
ATP phosphoribosyltransferase activity	1
ATP synthesis coupled proton transport	1
ATP:ADP antiporter activity	2
ATPase activity	25
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism	4
ATP-binding cassette (ABC) transporter activity	30
ATP-dependent proteolysis	1
autophagy	1
auxin polar transport	2
beta-amylase activity	2
binding	2
biosynthesis	7
branched chain family amino acid biosynthesis	1
brassinosteroid biosynthesis	1
C-5 sterol desaturase activity	1
C-8 sterol isomerase activity	1
calcium ion binding	9
calcium ion sensing	2
calcium ion storage activity	1
calcium-dependent phospholipid binding	1
calcium-mediated signaling	1
calmodulin binding	10
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	2
carbohydrate binding	1
carbohydrate metabolism	9
carbon utilization	1
carboxy-lyase activity	2
carboxypeptidase A activity	1
carotene 7,8-desaturase activity	1
carotene biosynthesis	3
carrier activity	1
caspase activity	1
catalytic activity	24
cation transport	1
cationic amino acid transporter activity	2
cation-transporting ATPase activity	2
CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity	1
cell adhesion	2
cell adhesion receptor activity	1
cell cycle	6
cell elongation	2
cell growth and/or maintenance	8
cell plate	2
cell surface	1
cell surface receptor linked signal transduction	1
cell wall (sensu Magnoliophyta)	2
cellular morphogenesis during differentiation	2
cellular response to glucose starvation	1
cellular_component unknown	33
cellulose biosynthesis	1
chalcone isomerase activity	1
chaperone activity	13
chlorophyll binding	1
chlorophyll catabolism	1
chlorophyll synthetase activity	2
chloroplast	116
chloroplast envelope	6
chloroplast inner membrane	5
chloroplast stroma	12
chloroplast stromal thylakoid	2
chromatin	1
chromosome organization and biogenesis (sensu Eukarya)	1
cinnamyl-alcohol dehydrogenase activity	3
citrate (pro-3S)-lyase activity	2
clathrin binding	8
clathrin vesicle coat	5
cleavage/polyadenylation specificity factor activity	1
COPI vesicle coat	5
copper chaperone activity	1
copper ion binding	3
coproporphyrinogen oxidase activity	1
cortical microtubule (sensu Viridiplantae)	2
C-terminal protein amino acid methylation	1
cuticle biosynthesis	1
cyclin binding	1
cyclin-dependent protein kinase activity	2
cyclin-dependent protein kinase regulator activity	2
cystathionine beta-lyase activity	1
cysteine biosynthesis	1
cysteine protease inhibitor activity	1
cysteine synthase activity	1
cysteine-type peptidase activity	7
cysteinyl-tRNA aminoacylation	1
cytochrome biogenesis	3
cytokinesis	1
cytokinesis by cell plate formation	1
cytoplasm	9
cytoskeleton	44
cytoskeleton organization and biogenesis	1
cytosol	2
cytosolic large ribosomal subunit (sensu Bacteria)	6
damaged DNA binding	1
de novo' IMP biosynthesis	1
defense response	1
defense response to bacteria	2
defense/immunity protein activity	1
delta1-pyrroline-5-carboxylate synthetase activity	1
deoxyribonuclease activity	1
development	3
diacylglycerol kinase activity	1
dihydrolipoyllysine-residue acetyltransferase activity	4
dihydropyrimidinase activity	1
disulfide oxidoreductase activity	2
DNA binding	121
DNA dependent ATPase activity	8
DNA endoreduplication	1
DNA helicase activity	2
DNA methylation	7
DNA polymerase processivity factor activity	1
DNA recombination	3
DNA repair	2
DNA replication	7
DNA replication, priming	1
DNA unwinding	1
DNA-directed DNA polymerase activity	2
DNA-directed RNA polymerase III complex	1
double-stranded DNA binding	1
drug transporter activity	1
dTDP-glucose 4,6-dehydratase activity	3
electron carrier activity	5
electron transport	42
electron transporter activity	1
electron transporter, transferring electrons within CoQH2-cytochrome c reductase complex activity	1
embryonic development	3
embryonic development (sensu Magnoliophyta)	1
endomembrane system	82
endoplasmic reticulum	3
endoplasmic reticulum membrane	2
endosome membrane	1
endosperm development	2
enoyl-CoA hydratase activity	3
extracellular	11
farnesyltranstransferase activity	1
fatty acid beta-oxidation	1
fatty acid biosynthesis	4
fatty-acid synthase activity	2
ferredoxin reductase activity	1
ferredoxin:thioredoxin reductase activity	1
ferric iron binding	2
FK506 binding	2
flower development	3
FMN binding	1
folic acid and derivative biosynthesis	3
formaldehyde dehydrogenase (glutathione) activity	2
fructose metabolism	1
fructose-bisphosphatase activity	1
galactolipid galactosyltransferase activity	1
galactose biosynthesis	1
galactose metabolism	4
gamma-glutamyl hydrolase activity	1
GDP-mannose transporter activity	1
geranylgeranyl reductase activity	2
geranylgeranyl-diphosphate geranylgeranyltransferase activity	2
gluconeogenesis	1
glucose metabolism	1
glucose-1-phosphate adenylyltransferase activity	3
glutamate synthase (ferredoxin) activity	6
glutamate synthase (NADH) activity	2
glutamate-1-semialdehyde 2,1-aminomutase activity	1
glutamate-ammonia ligase activity	5
glutamine-tRNA ligase activity	1
glutamyl-tRNA aminoacylation	3
glutamyl-tRNA reductase activity	2
glutathione peroxidase activity	4
glutathione synthase activity	1
glutathione transferase activity	5
glutathione-disulfide reductase activity	1
glyceraldehyde-3-phosphate dehydrogenase activity	4
glycerol-3-phosphate dehydrogenase complex	2
glycerol-3-phosphate O-acyltransferase activity	1
glycine catabolism	1
glycine decarboxylation via glycine cleavage system	3
glycine transaminase activity	3
glycolysis	16
glycyl-tRNA aminoacylation	3
glyoxysome	1
glyphosate metabolism	1
GMP synthase (glutamine-hydrolyzing) activity	1
Golgi apparatus	1
Golgi stack	1
G-protein coupled receptor activity	4
G-protein coupled receptor protein signaling pathway	24
GTP binding	19
GTPase activity	1
guanosine tetraphosphate (5'-ppGpp-3') metabolism	2
guanyl-nucleotide exchange factor activity	1
heat shock protein activity	3
heme oxidation	1
heterotrimeric G-protein complex	1
high affinity iron ion transport	1
histidine biosynthesis	1
histidine phosphotransfer kinase activity	3
histidinol-phosphate transaminase activity	2
histone acetyltransferase activity	2
histone deacetylation	2
hydrogen peroxide catabolism	1
hydrogen-exporting ATPase activity, phosphorylative mechanism	1
hydrogen-translocating V-type ATPase activity	6
hydrogen-transporting ATP synthase activity, rotational mechanism	6
hydrogen-transporting ATPase activity, rotational mechanism	1
hydrolase activity	24
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	1
hydrolase activity, acting on ester bonds	1
hydrolase activity, hydrolyzing O-glycosyl compounds	10
hydro-lyase activity	1
hydroxymethyl-, formyl- and related transferase activity	2
hypusine biosynthesis from peptidyl-lysine	1
immune response	2
inorganic anion transporter activity	1
inositol/phosphatidylinositol kinase activity	5
inositol/phosphatidylinositol phosphatase activity	1
integral to membrane	30
intracellular	5
intracellular protein transport	7
intracellular signaling cascade	1
intracellular transporter activity	1
iron-sulfur cluster assembly	1
isomerase activity	2
isopentenyl diphosphate biosynthesis, mevalonate independent	3
kinase activity	122
kinase activity	52
kinesin motor activity	11
lactoylglutathione lyase activity	1
large ribosomal subunit	13
L-ascorbate peroxidase activity	1
leaf morphogenesis	1
leucine biosynthesis	2
leucine catabolism	2
light-harvesting complex	23
lipid binding	13
lipid metabolism	5
lipid storage	3
lipoxygenase activity	1
L-serine metabolism	3
lyase activity	2
lycopene beta cyclase activity	2
lycopene epsilon cyclase activity	1
lysine biosynthesis via diaminopimelate	4
magnesium chelatase activity	3
magnesium protoporphyrin IX methyltransferase activity	1
malate dehydrogenase activity	4
malate transport	5
mannitol dehydrogenase activity	2
MAPKKK cascade	1
mature ribosome assembly	2
membrane	62
membrane fraction	3
membrane fusion	1
metabolism	35
metal ion binding	3
metal ion transport	1
metal ion transporter activity	4
metalloexopeptidase activity	4
metallopeptidase activity	3
methionine biosynthesis	3
methionine metabolism	1
methionyl aminopeptidase activity	3
methionyl-tRNA aminoacylation	1
methylcrotonoyl-CoA carboxylase activity	2
methyltransferase activity	5
microsome	1
microtubule binding	1
microtubule-based process	2
mitochondrial inner membrane	1
mitochondrial matrix	1
mitochondrial outer membrane translocase complex	1
mitochondrion	35
mitosis	1
molecular_function unknown	124
Mo-molybdopterin cofactor biosynthesis	6
motor activity	1
mRNA binding	1
mRNA catabolism, nonsense-mediated	1
mRNA polyadenylation	1
mRNA processing	39
mRNA splice site selection	1
mRNA splicing	9
myosin	1
NADH dehydrogenase (ubiquinone) activity	5
NADH dehydrogenase activity	5
negative regulation of flower development	1
negative regulation of photomorphogenesis	2
negative regulation of transcription	2
nicotinamide adenine dinucleotide biosynthesis	3
nitrate reductase activity	2
nitrogen metabolism	1
N-linked glycosylation	1
N-linked glycosylation via asparagine	2
nuclear matrix	1
nuclear membrane	2
nuclease activity	1
nucleic acid binding	16
nucleobase, nucleoside, nucleotide and nucleic acid metabolism	1
nucleobase, nucleoside, nucleotide and nucleic acid transport	1
nucleocytoplasmic transport	1
nucleolus	1
nucleoside metabolism	3
nucleosome assembly	2
nucleotide binding	29
nucleotide-sugar metabolism	3
nucleotidyltransferase activity	5
nucleus	56
nutrient reservoir activity	8
oligosaccharyl transferase activity	1
omega-3 fatty acid desaturase activity	2
omega-6 fatty acid desaturase activity	2
organic anion transporter activity	11
ornithine-oxo-acid transaminase activity	2
ossification	1
oxidoreductase activity	26
oxidoreductase activity, acting on NADH or NADPH	1
oxidoreductase activity, acting on NADH or NADPH, NAD or NADP as acceptor	3
oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor	2
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors	1
oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor	2
oxidoreductase activity, acting on sulfur group of donors, other acceptors	1
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor	2
oxidoreductase activity, acting on the CH-CH group of donors	2
oxoglutarate dehydrogenase (succinyl-transferring) activity	2
oxygen binding	4
palmitoyl-(protein) hydrolase activity	1
pectinesterase inhibitor activity	8
peptidase activity	15
peptide deformylase activity	1
peptidyl-proline hydroxylation to 4-hydroxy-L-proline	9
peptidyl-prolyl cis-trans isomerase activity	1
peroxisomal membrane	7
peroxisome	5
phenylalanyl-tRNA aminoacylation	2
phenylpropanoid metabolism	2
phosphate transporter activity	2
phosphatidate cytidylyltransferase activity	1
phosphoadenylyl-sulfate reductase (thioredoxin) activity	1
phosphoenolpyruvate carboxylase activity	5
phosphogluconate dehydrogenase (decarboxylating) activity	3
phosphoglycolate phosphatase activity	1
phospholipase A2 activity	1
phospholipase C activity	1
phospholipid biosynthesis	2
phosphopyruvate hydratase activity	2
phosphoribosyl-AMP cyclohydrolase activity	1
phosphoribosylformylglycinamidine cyclo-ligase activity	2
phosphoric diester hydrolase activity	2
phosphoric ester hydrolase activity	1
phosphorylase kinase activity	1
phosphorylation	1
phosphoserine phosphatase activity	5
phosphotransferase activity, phosphate group as acceptor	1
photomorphogenesis	1
photosynthesis	5
photosynthetic electron transport	2
photosystem I reaction center	2
phototropism	1
phragmoplast	4
phycobilisome	2
plasma membrane	3
plastid	4
plastid ribosome	2
plastid stroma	2
plastoquinol-plastocyanin reductase activity	2
pollen tube growth	1
porphobilinogen synthase activity	1
potassium ion transport	2
potassium:hydrogen antiporter activity	1
prefoldin complex	1
pre-mRNA splicing factor activity	16
prenyltransferase activity	1
proteasome regulatory particle, base subcomplex (sensu Eukarya)	8
proteasome regulatory particle, lid subcomplex (sensu Eukarya)	6
protein amino acid dephosphorylation	2
protein amino acid phosphorylation	9
protein binding	83
protein binding, bridging	1
protein biosynthesis	71
protein degradation tagging activity	3
protein disulfide oxidoreductase activity	1
protein farnesyltransferase complex	2
protein folding	32
protein geranylgeranylation	1
protein kinase activity	9
protein kinase CK2 regulator activity	1
protein methyltransferase activity	1
protein phosphatase type 1 activity	1
protein phosphatase type 2A activity	2
protein phosphatase type 2A regulator activity	3
protein phosphatase type 2C activity	9
protein secretion	2
protein serine/threonine kinase activity	11
protein serine/threonine phosphatase activity	8
protein sumoylation	1
protein targeting	1
protein threonine/tyrosine kinase activity	1
protein translocase activity	5
protein transport	4
protein transporter activity	11
protein tyrosine/serine/threonine phosphatase activity	3
protein ubiquitination	3
protein-arginine N-methyltransferase activity	2
protein-histidine kinase activity	2
protein-methionine-S-oxide reductase activity	4
protein-tyrosine kinase activity	6
proteolysis and peptidolysis	38
protochlorophyllide reductase activity	4
proton transport	1
proton-transporting ATP synthase complex, coupling factor F(o) (sensu Bacteria)	1
pseudouridine synthase activity	1
purine base metabolism	2
purine nucleotide biosynthesis	3
purine ribonucleoside monophosphate biosynthesis	1
purine ribonucleotide biosynthesis	3
pyridine nucleotide biosynthesis	1
pyrimidine base metabolism	1
pyrophosphatase activity	2
pyruvate decarboxylase activity	1
pyruvate dehydrogenase (acetyl-transferring) activity	3
pyruvate, water dikinase activity	1
RAN protein binding	1
receptor activity	2
reductive pentose-phosphate cycle	1
regulation of flower development	1
regulation of meristem size	3
regulation of transcription, DNA-dependent	16
regulation of translational initiation	1
removal of superoxide radicals	3
response to abscisic acid stimulus	1
response to aluminum ion	1
response to ethylene stimulus	1
response to heat	7
response to pathogenic bacteria (incompatible interaction)	5
response to pathogenic fungi	7
response to stress	4
response to UV	4
retrograde (endosome to Golgi) transport	3
rhodopsin-like receptor activity	49
ribonuclease activity	2
ribonucleoside-diphosphate reductase activity	1
ribose-5-phosphate isomerase activity	2
ribosome	3
ribosome biogenesis	29
ribosome biogenesis and assembly	1
ribulose-bisphosphate carboxylase activity	6
RNA binding	55
RNA helicase activity	4
RNA methyltransferase activity	4
RNA modification	1
RNA processing	3
RNA-directed DNA polymerase activity	1
rRNA processing	1
S-adenosylmethionine biosynthesis	1
S-adenosylmethionine-dependent methyltransferase activity	2
salinity response	1
SCF ubiquitin ligase complex	1
selenium binding	1
serine-type endopeptidase activity	21
serine-type endopeptidase inhibitor activity	1
serine-type peptidase activity	4
seryl-tRNA aminoacylation	2
sex determination	1
shoot morphogenesis	1
signal transducer activity	1
signal transduction	7
signal-recognition-particle GTPase activity	3
single-stranded DNA binding	1
small ribosomal subunit	6
SNF1A/AMP-activated protein kinase activity	2
sodium ion transport	1
solute:sodium symporter activity	2
SOS response	1
sphingolipid biosynthesis	1
spliceosome complex	1
squalene monooxygenase activity	1
starch catabolism	3
steroid biosynthesis	5
steroid metabolism	2
sterol carrier activity	1
structural constituent of cell wall	59
structural constituent of cytoskeleton	3
structural constituent of ribosome	10
structural molecule activity	14
subtilase activity	2
sugar binding	1
sulfate transporter activity	1
sulfite reductase activity	2
terpenoid biosynthesis	2
thiamin biosynthesis	2
thioglucosidase binding activity	2
thiol-disulfide exchange intermediate activity	16
threonine synthase activity	2
threonyl-tRNA aminoacylation	4
thylakoid	2
thylakoid (sensu Viridiplantae)	3
thylakoid lumen (sensu Viridiplantae)	28
thylakoid membrane (sensu Viridiplantae)	4
thylakoid membrane organization and biogenesis	1
thymidylate kinase activity	1
transaldolase activity	2
transaminase activity	5
transcription	22
transcription coactivator activity	1
transcription factor activity	27
transcription regulator activity	3
transferase activity	2
transferase activity, transferring glycosyl groups	16
transferase activity, transferring phosphorus-containing groups	1
trans-Golgi network transport vesicle	1
transketolase activity	2
translation elongation factor activity	11
translation factor activity, nucleic acid binding	4
translation initiation factor activity	7
translational elongation	6
translational initiation	12
translational initiation	8
translational termination	2
transport	25
transporter activity	4
transposase activity	1
tricarboxylic acid cycle	2
triose phosphate transport	5
tRNA aminoacylation for protein translation	16
tRNA processing	1
tyrosine catabolism	1
ubiquinol-cytochrome-c reductase activity	2
ubiquitin	1
ubiquitin conjugating enzyme activity	14
ubiquitin cycle	1
ubiquitin-dependent protein catabolism	5
ubiquitin-protein ligase activity	1
ubiquitin-specific protease activity	3
UDP-glucuronate decarboxylase activity	1
UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity	1
UDPsulfoquinovose synthase activity	1
uracil salvage	1
uridine kinase activity	1
uridylate kinase activity	1
uroporphyrinogen decarboxylase activity	3
vacuolar membrane	1
vacuolar membrane (sensu Magnoliophyta)	5
vesicle-mediated transport	2
viral capsid	1
viral replication complex formation and maintenance	1
vision	7
vitamin B2 biosynthesis	1
voltage-dependent ion-selective channel activity	2
voltage-gated chloride channel activity	1
xanthine dehydrogenase activity	2
xenobiotic-transporting ATPase activity	2
X-Pro dipeptidase activity	1
zeaxanthin epoxidase activity	2
zinc ion binding	9
zinc ion transport	2